• Journal of Animal Science and Technology
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• v.57 no.5
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• pp.18.1-18.8
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• 2015

Background: Quantitative real time reverse transcription PCR (qRT-PCR) is one of the most important techniques for gene-expression analysis in molecular based studies. Selecting a proper internal control gene for normalizing data is a crucial step in gene expression analysis via this method. The expression levels of reference genes should be remained constant among cells in different tissues. However, it seems that the location of cells in different tissues might influence their expression. The purpose of this study was to determine whether the source of mesenchymal stem cells (MSCs) has any effect on expression level of three common reference genes (GAPDH, ${\beta}$-actin and ${\beta}2$-microglobulin) in equine marrow- and adipose-derived undifferentiated MSCs and consequently their reliability for comparative qRT-PCR. Materials and methods: Adipose tissue (AT) and bone marrow (BM) samples were harvested from 3 mares. MSCs were isolated and cultured until passage 3 (P3). Total RNA of P3 cells was extracted for cDNA synthesis. The generated cDNAs were analyzed by quantitative real-time PCR. The PCR reactions were ended with a melting curve analysis to verify the specificity of amplicon. Results: The expression levels of GAPDH were significantly different between AT- and BM-derived MSCs (p < 0.05). Differences in expression level of ${\beta}$-actin (P < 0.001) and B2M (P < 0.006.) between MSCs derived from AT and BM were substantially higher than GAPDH. In addition, the fold change in expression levels of GAPDH, ${\beta}$-actin and B2M in AT-derived MSCs compared to BM-derived MSCs were 2.38, 6.76 and 7.76, respectively. Conclusion: This study demonstrated that GAPDH and especially ${\beta}$-actin and B2M express in different levels in equine AT- and BM-derived MSCs. Thus they cannot be considered as reliable reference genes for comparative quantitative gene expression analysis in MSCs derived from equine bone marrow and adipose tissue.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.8 no.3
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• pp.181-188
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• 2010

Metal surface decontamination characteristics were investigated by using a laser ablation method. A second harmonic generation of a Q-switched Nd:YAG laser with a wave length of 532 nm, a pulse energy of 150 mJ and a pulse width of 5 ns was employed to assess the decontamination performance for metal surfaces contaminated with $CsNO_3$, $Co(NH_4)_2(SO_4)_2$, $Eu_2O_3$ and $CeO_2$. The ablation behavior was investigated for the decontamination variables such as a number of laser shots, laser fluence and an irradiation angle. Their optimum values were found to be 8, 13.3 J/$cm^2$ and $30^{\circ}$, respectively. The decontamination efficiency was different depending on the kinds of the contaminated ions, due to their different melting and boiling points and was in the order: $CsNO_3>Co(NH_4)_2(SO_4)_2>Eu_2O_3>CeO_2$. We also evaluated a correlation between the metal ablation thickness and the number of laser shots for the different laser fluences.

• Resources Recycling
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• v.18 no.1
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• pp.38-43
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• 2009

In this study, binderless consolidation processes of ultra foe Si powder, by-products of making high purity poly-Si in the current method, were systematically investigated for use as economical solar-grade feedstock. The average diameter of the silicon powder was $7.8{\mu}m$. The main contaminants of the fine silicon powder were $SiO_2$ type oxide and humidity. The chemical pretreatment using the HF solution was observed to be effective for the improvement of the compactability of the silicon powder and the density ratio and the strength of the silicon powder compacts. The yield of the binder-free consolidation process increased by 20% under a vacuum condition. In as-received state, the silicon powder were not pure enough to be used as solar grade feed-stock material. After the dry chemical treatments, a sufficiently high purity above solar-grade was able to be achieved.

• Proceedings of the Korean Vacuum Society Conference
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• 2000.02a
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• pp.113-113
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• 2000

직접천이형 wide band gap(3.4eV) 반도체중의 하나인 GaN를 청색 및 자외선 laser diode, 고출력 전자장비 등으로 응용하기 위해서는 낮은 접합저항을 갖는 Ohmic contact이 선행되어야 한다. 그러나 만족할만한 p-type GaN의 Ohmic contact은 아직 실현되고 있지 못하며, 이는 GaN와 접합 금속과의 구체적인 반응의 연구를 필요로 한다. 본 연구에서 앞서 Pt, Pt, Ni등의 late transition metal을 p-GaN에 접합시킨 결과 이들은 접합 당시 비교적 평탄하나 후열 처리과정에서 비교적 낮은 온도에서 기판과 열팽창계수의 차이로 인하여 평탄성을 잃어버리면서 barrier height가 증가한다는 사실을 확인하였다. 따라서 본 연구에서는 이러한 열적 불안정성을 극복하기 위하여 Ni과 Pd를 차례로 증착하고 가열하면서 interfacial reaction, film morphology, Fermi level의 움직임을 monchromatic XPS(x-ray photoelectron spectroscopy) 와 SAM(scanning Auger microscopy) 그리고 ex-situ AFM을 이용하여 밝히고자 하였다. 특히 후열처리에 의한 계면 반응에 수반되는 구성 금속원소 간의 합금현상과 금속 층의 평탄성이 밀접한 관계가 있다는 것을 확인하였다. 이러한 합금과정에서 나타나는 금속원소들의 중심 준위의 이동을 체계적으로 규명하기 위해서 Pd1-xNix와 Pd1-xGax 합금들의 표준시료를 arc melting method로 만들어 농도에 따른 금속원소들의 중심 준위의 이동을 측정하여, Pd/Ni/p-GaN 및 Ni/Pd/p-GaN 계에서 열처리 온도에 따른 interfacial reaction을 확인하였다. 그 결과 두 계가 상온에서 nitride 및 alloy를 형성하지 않고 고르게 증착되고, 열처리 온도를 40$0^{\circ}C$에서 $650^{\circ}C$까지 증가시킴에 따라 계면반응의 부산물인 metallic Ga은 증가하고 있으마 nitride는 여전히 형성되지 않는 것을 확인하였다. 증착당시 Ni이 계면에 있는 Pd/Ni/p-GaN의 경우에는 52$0^{\circ}C$까지의 열처리에 의하여 Ni과 Pd가 골고루 섞이고 그 평탄성도 유지되고 barier height의 변화도 없었다. 더 높은 $650^{\circ}C$ 가열에 의해서는 surface free energy가 작은 Ga의 활발한 편석 현상으로 인해 표면은 Ga이 풍부한 Pd-Ga의 합금층으로 덮이고, 동시에 작은 pinhole들이 발생하며 barrier height도 0.3eV 가량 증가하게 된다. 반면에 증착당시 Pd이 계면에 있는 Ni/Pd/p-GaN의 경우에는 40$0^{\circ}C$의 가열까지는 두 금속이 그들 계면에서부터 섞이나, 52$0^{\circ}C$의 가열에 의해 이미 barrier height가 0.2eV 가량 증가하기 시작하였다. 더 높은 $650^{\circ}C$가열에 의해서는 커다란 pinhole, 0.5eV 가량의 barrier height 증가, Pd clustering이 동시에 관찰되었다. 따라서 Ni과 Pd의 일함수는 물론 thermal expansion coefficient가 거의 같으며 surface free energy도 거의 일치한다는 점을 감안하면, 이렇게 뚜렷한 열적 안정성의 차이는 GaN와 contact metal과의 반응시작 온도(disruption onset temperature)의 차이에 기인함을 알 수 있었다. 즉 계면에서의 반응에 의해 편석되는 Ga에 의해 박막의 strain이 이완되면, pinhole 등의 박막결함이 줄어 들고, 이는 계면의 N의 out-diffusion을 방지하여 p-type GaN의 barrier height 증가를 막게 된다.

• Resources Recycling
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• v.21 no.1
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• pp.30-40
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• 2012

Electric arc furnace slag is made in ironworks during steel refining, it is been increasing chemical and physical resistibility using ageing method of unstable state of melting steel slag for using concrete's fine aggregates. Which is been changing stable molecular structure of aggregates, it restrains moving of ion and molecule. In Korea, KS F 4571 has been prepared for using the electric arc furnace oxidizing slag to concrete aggregates(EFS). In this study, Electric arc furnace oxidizing slag is used in the PRCC(Polymer Rapid setting Cement Concrete) which is applied a bridge pavement of rehabilitation, largely. The results showed that the increment of compressive strength development by 10- 20%. The flexural strength of EFS-Con increased greatly as the electric arc furnace oxidizing slag changed. The compressive strength and flexural strength developed enough for opening the overlayed EFS-Con to the traffic after 4 hours of EFS-Con placement. The permeability of EFS-Con was evaluated as negligible due to its very low charge passed. Thus, EFS-Con could be used at repairing or overlaying the concrete at fast-track job sites.

• Korean Journal of Remote Sensing
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• v.35 no.5_1
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• pp.625-636
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• 2019

Soil moisture is a representative factor that plays a key role in hydrological cycle. It is involved in the interaction between atmosphere and land surface, and is used in fields such as agriculture and water resources. Advanced Microwave Scanning Radiometer 2 (AMSR2), Advanced SCATterometer (ASCAT), and European Space Agency Climate Change Initiative (ESACCI) data were used to analyze the applicability and uncertainty of satellite soil moisture product in the Korean peninsula. Cumulative distribution function (CDF) matching and triple collocation (TC) analysis were carried out to investigate uncertainty and correction of satellite soil moisture data. Comparisons of pre-calibration satellite soil moisture data with the Automated Agriculture Observing System (AAOS) indicated that ESACCI and ASCAT data reflect the trend of AAOS well. On the other hand, AMSR2 satellite data showed overestimated values during the freezing period. Correction of satellite soil moisture data using CDF matching improved the error and correlation compared to those before correction. Finally, uncertainty analysis of soil moisture was carried out using TC method. Clearly, the uncertainty of the satellite soil moisture, corrected by CDF matching, was diminished in both freezing and thawing periods. Overall, it is expected that using ASCAT and ESACCI rather than AMSR2 soil moisture data will give more accurate soil moisture information when correction is performed on the Korean peninsula.

• The Plant Pathology Journal
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• v.35 no.1
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• pp.11-18
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• 2019

Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined with three programs-geNorm, Norm Finder, and Best Keeper. $TUB{\beta}$ was identified as the most stable reference gene. Furthermore, the exopolygalacturonase gene (ExoPG) was selected to verify the reliability of $TUB{\beta}$ expression. The expression profile of ExoPG assessed using $TUB{\beta}$ agreed with the results of digital gene expression analysis by RNA-Seq. This study is the first systematic exploration of the optimal reference genes in the infection process of Gaeumannomyces graminis var. tritici.

• Economic and Environmental Geology
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• v.51 no.6
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• pp.485-492
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• 2018

About 300 thousand tones of oyster shells are produced annually and, thus, their massive recycling methods are required. Recently, a method, utilizing them as wet desulfurization materials after removal of organic matters and changing $CaCO_3$ phase into CaO through calcination, is under consideration. This study investigates the mineralogical changes (specific surface area, phase changes, surface state, etc.) of oyster shells by calcination and their characteristics were compared with those of limestone. Uncalcined oyster shells showed the higher specific surface area than limestone because the former are composed of platy and columnar structures. In contrast, investigated limestone showed a dense structure. The phase change of oyster shells occurred at lower temperature than that of limestone. The specific surface area of oyster shell decreased significantly after calcination while limestone depicted a drastic increase. Small amount of Na contained in oyster shell was suggested as the cause of this phenomenon; in that, it acted as a flux causing melting and sintering of oyster materials at lower temperature. Because of this, an additional phenomenon was observed that a part of shell materials remained untransformed even at higher calcination temperature and after longer treatment period due to the sintered surface, which covers the rest parts. Further studies investigating the effect of this phenomena from the perspective of desulfurization is required.

• Design & Manufacturing
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• v.13 no.2
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• pp.17-21
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• 2019

In this study, selective laser sintered 3D printing mold core and metal core were used to investigate the difference of the thickness shrinkage from the gate of the injection molded part at a constant interval. SLS 3D printing mold core was made of nylon-based PA2200 powder and the metal core was manufactured by conventional machining method. As the PA2200 powder material has low strength, thermal conductivity and high specific heat characteristics compared with metal, molding conditions were set with the consideration of molten temperature and injection pressure. Crystalline resin(PP) and amorphous resin(PS) with low melting temperature and viscosity were selected for the injection molding experiment. Cooling time for processing condition was selected by checking the temperature change of the cores with a cavity temperature sensor. The cooling time of the 3D printing core was required a longer time than that of the metal core. The thickness shrinkage of the molded part compared to the core depth was measured from the gate by a constant interval. It was shown that the thickness shrinkage of the 3D printing core was 2.02 ~ 4.34% larger than that of metal core. In additions, in the case of metal core, thickness shrinkage was increased with distance from the gate, on the contrary, in the case of polymer core showed reversed aspect.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.12 no.4
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• pp.336-342
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• 2019

With the entry of an aging society, the average life span of accreditation has been extended. Therefore, interest in the appearance of men and women in modern society has increased. It is the wrinkles of the face that can judge the most outwardly. People tend to have various kinds of treatments to have a clean, wrinkle-free and resilient healthy skin regardless of sex. There is a lot of practice of lifting procedures in one of the procedures. A suture using a melting thread is a method of lifting by squeezing it into the skin as a non-incision type centering on a region where the thread can be fixed in the skin by injecting it into the subcutaneous fat layer. To evaluate the lifting efficacy of Cog - type suture for the improvement of skin wrinkles, preclinical experiments were conducted. We developed a wrinkle evaluation program using Labview. Data from preclinical experiments were used at 8 weeks after suturing. The average wrinkle depth was 415.6 mm in the control group. At 8 weeks, the depth of wrinkles was deepened to 888.3mm due to the aging process of the control group. On the other hand, the depth of the wrinkles before surgery was 640.3 mm in the suture group. It was confirmed that the depth of wrinkles decreased to 566.5mm at 8th week after the suture operation.