• Asian-Australasian Journal of Animal Sciences
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• 제26권11호
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• pp.1598-1608
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• 2013

An optimum dietary carbohydrate content is important for maximum fish growth. In this study, we fed Wuchang bream (Megalobrama amblycephala) with either control diet (30.42%) or high carbohydrate diet (52.92%) for 90 d. Fish were fed to apparent satiation three times daily in an aquarium with automatic temperature control and circulated water. Growth performance, plasma biochemical parameters, hepatic morphology and enzyme activities were determined. It was shown that compared to fish fed control diet, fish fed high carbohydrate diet had higher plasma triglyceride and cortisol levels for d 90, and lower alkaline phosphatase level for d 45, lower hepatic superoxide dismutase and total antioxidative capacity for d 90, higher malondialdehyde for d 45 and glycogen content for d 45 and 90 (p<0.05). Histological and transmission electron microscopy studies showed that hepatocytes of fish fed high carbohydrate diet contained large lipid droplets, causing displacement of cellular organelles to periphery of hepatocytes. The relative level of hepatic heat shock protein 70 (HSP70) mRNA of Wuchang bream fed high carbohydrate diet was significantly higher than that of fish fed the control diet for 90 d (p<0.05). These changes led to decreased specific growth rate and increased feed conversion ratio (p<0.05). Upon hypoxia challenge, fish fed high carbohydrate diet had higher cumulative mortality than those fed the control diet (p<0.05). These results suggested that high dietary carbohydrate (52.92%) was detrimental to the growth performance and health of Wuchang bream.

• 한국어류학회지
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• 제30권3호
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• pp.144-154
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• 2018

서울시 한강 어류군집 특성 분석을 위해 2016년 6월부터 2017년 3월까지 조사하였다. 조사기간 동안 5개 조사지점에서 출현한 어종은 총 17과 56종이었다. 기수역에 주로 서식하는 웅어 (Coilia nasus), 밀자개 (Leiocassis nitidus), 점농어 (Lateolabrax maculata), 강주걱양태(Repomucenus olidus), 꺽정이 (Trachidermus fasciatus), 숭어 (Mugil cephalus), 가숭어 (Chelon haematocheilus), 풀망둑 (Synechogobius hastus), 민물두줄망둑 (Tridentiger bifasciatus), 황복 (Takifugu obscurus) 등이 출현하였다. 회유성 어종인 뱀장어 (Anguilla japonica), 웅어, 은어(Plecoglossus altivelis), 빙어(Hypomesus nipponensis), 황복 등이 출현하였다. 한반도 고유종은 11종 (17.9%)로 매우 낮은 비율을 차지하였다. 국외에서 도입된 어종은 유럽잉어 (Cyprinus carpio nudus), 떡붕어 (Carassius cuvieri), 백련어 (Hypophthalmichthys molitrix), 단두어 (Megalobrama amblycephala), 블루길(Lepomis macrochirus), 배스 (Micropterus salmoides) 등 6종 (10.7%)이었다. 각 조사지점별 우점종은 가시납지리 (Acheilognathus gracilis, St. 1)와 누치 (Hemibarbus labeo, St. 2, 3, 4, 5)이었다. 수질 내성도 guild에서 민감종의 상대풍부도는 3종 (7.1%), 중간종은 24종 (42.9%), 내성종은 28종 (50.0%)로 내성종이 매우 높았다. 섭식특성 gulid에서 충식성은 20종 (35.7%), 잡식성 18종 (32.1%), 육식성 14종(25.0%), 초식성 4종(7.1%)으로 충식성과 잡식성 어종이 매우 풍부하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제28권2호
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• pp.207-214
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• 2015

The effects of high carbohydrate diet on growth, serum physiological response, and hepatic heat shock protein 70 expression in Wuchang bream were determined at $25^{\circ}C$ and $30^{\circ}C$. At each temperature, the fish fed the control diet (31% CHO) had significantly higher weight gain, specific growth rate, protein efficiency ratio and hepatic glucose-6-phosphatase activities, lower feed conversion ratio and hepatosomatic index (HSI), whole crude lipid, serum glucose, hepatic glucokinase (GK) activity than those fed the high-carbohydrate diet (47% CHO) (p<0.05). The fish reared at $25^{\circ}C$ had significantly higher whole body crude protein and ash, serum cholesterol and triglyceride, hepatic G-6-Pase activity, lower glycogen content and relative levels of hepatic growth hormone (GH) gene expression than those reared at $30^{\circ}C$ (p<0.05). Significant interaction between temperature and diet was found for HSI, condition factor, hepatic GK activity and the relative levels of hepatic GH gene expression (p<0.05).

• Journal of Animal Science and Technology
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• 제57권12호
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• pp.44.1-44.9
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• 2015

Background: Heat shock proteins play an important role in protection from stress stimuli and metabolic insults in almost all organisms. Methods: In this study, computational tools were used to deeply analyse the physicochemical characteristics and, using homology modelling, reliably predict the tertiary structure of the blunt snout bream (Ma-) Hsp70 and Hsc70 proteins. Derived three-dimensional models were then used to predict the function of the proteins. Results: Previously published predictions regarding the protein length, molecular weight, theoretical isoelectric point and total number of positive and negative residues were corroborated. Among the new findings are: the extinction coefficient (33725/33350 and 35090/34840 - Ma-Hsp70/ Ma-Hsc70, respectively), instability index (33.68/35.56 - both stable), aliphatic index (83.44/80.23 - both very stable), half-life estimates (both relatively stable), grand average of hydropathicity (-0.431/-0.473 - both hydrophilic) and amino acid composition (alanine-lysine-glycine/glycine-lysine-aspartic acid were the most abundant, no disulphide bonds, the N-terminal of both proteins was methionine). Homology modelling was performed by SWISS-MODEL program and the proposed model was evaluated as highly reliable based on PROCHECK's Ramachandran plot, ERRAT, PROVE, Verify 3D, ProQ and ProSA analyses. Conclusions: The research revealed a high structural similarity to Hsp70 and Hsc70 proteins from several taxonomically distant animal species, corroborating a remarkably high level of evolutionary conservation among the members of this protein family. Functional annotation based on structural similarity provides a reliable additional indirect evidence for a high level of functional conservation of these two genes/proteins in blunt snout bream, but it is not sensitive enough to functionally distinguish the two isoforms.