• Title/Summary/Keyword: Medium effect

검색결과 5,966건 처리시간 0.031초

톨 페스큐의 종자배양에 있어서 식물체 재부노하에 미치는 몇가지 요인 (Factors Affecting Plant Regeneration from Seed-Derived Calli in Tall Fescue (Festuca arundinacea Schreb).)

  • 이효신;이승민;권용삼;이병현;조진기
    • 한국초지조사료학회지
    • /
    • 제21권2호
    • /
    • pp.45-48
    • /
    • 2001
  • In an effort to optimize tissue culture responses of tall fescue, the effects of basic medium and carbon sources on seed culturability and genotypic difference of plant regenerability were investigated. The MS basal medium was superior to $N_{5}$ 6/ and $B_{medium}$ in enhancing callus growth and plant regeneration. To determine the effect of carbon sources on plant regeneration, the seeds were cultured on medium with 30 g/L sucrose and maltose, respectively. Medium supplemented with 30 g/L sucrose resulted in regeneration of shoots from 50% of the calli. The genotypic difference in plant regenerability was obvious among five cultivars of tall fescue tested. \\`KY31\\` and \\`Hokuryo\\` showed to have higher regenerability with the frequency of 33% and 51%, respectively.

  • PDF

무혈청 배지로부터 scu-PA의 생산을 위한 배지의 재사용 공정에 관한 연구 (Medium Recycle Process for the Production of scu-PA from Serum Free Medium)

  • 김영남;박경유
    • KSBB Journal
    • /
    • 제8권1호
    • /
    • pp.17-22
    • /
    • 1993
  • $1.96{\times}10^{-5}$(IU/cell/hr) of specific scu-PA production rate was obtained from HEK cells in maintaining ca. $8{\times}10^{5}$(cells/ml) of maximum roll density at 10(ml/hr) of perfusion rate with recycling 20% serum free conditioned media. It can be compared to $4{\times}10^{6}$(cells/ml) of maximum cell density and $4.56{\times}10^{-4}$(IU/cell/hr) of specific production rate in cultivating cells with 1% serum containing medium. Thc conversion ratio of scu-PA to tc-UK increased up to 55% as the recycling ratio increased; however, recycling the used medium seemed to have least negative effect on cell growth. It also showed that the recycling process had definitive advantage of using serum free medium in perfusion cultivation of HEK cell line.

  • PDF

Effect of Supplement nutrition on the Mycelial Growth of Lentinus edodes

  • Yang, Jae-Kyung;Kim, Tae-Hong;Lim, Bu-Kug
    • Journal of the Korean Wood Science and Technology
    • /
    • 제31권6호
    • /
    • pp.60-66
    • /
    • 2003
  • Mycelial growth of L. edodes by supplement nutrition of softwood was studied on a sawdust medium. The sawdust used was from the following softwood species : Larix leptolepis, Pinus densiflora and Pinus koraiensis. The added nutritions consisted of carbon nutritions(sucrose, active carbon, xylose, glucose, paper pellet), nitrogen nutritions(potassium nitrate, ammonium chloride, asparagine, glutamic acid) and vegetable oil(rice bran oil). The sawdust medium was a mixture of 76% sawdust, 20% rice bran, 3% carbon nutrition, 0.4% nitrogen nutrition and 0.6% calcium carbonate. Following addition of carbon and nitrogen nutritions on the sawdust medium proved most suitable : L. leptolepis (glucose, glutamic acid), P. densiflora (active carbon, asparagine) and P. koraiensis (xylose, glutamic acid). The highest mycelial growth was obtained from sawdust medium of optimum condition with 97% of L. leptolepis, 110% of P. densiflora and 98% of P. koraiensis. This study has provided useful preliminary information for the cultivation of L. edodes.

Effect of two temperature on isotropic modified couple stress thermoelastic medium with and without energy dissipation

  • Lata, Parveen;Kaur, Harpreet
    • Geomechanics and Engineering
    • /
    • 제21권5호
    • /
    • pp.461-469
    • /
    • 2020
  • The objective of this paper is to study the deformation in a homogeneous isotropic modified couple stress thermoelastic medium with and without energy dissipation and with two temperatures due to thermal source and mechanical force. Laplace and Fourier transform techniques are applied to obtain the solutions of the governing equations. The displacement components, stress components, conductive temperature and couple stress are obtained in the transformed domain. Isothermal boundary and insulated boundary conditions are used to investigate the problem.The effect of two temperature and GN theory of type-II and type-III has been depicted graphically on the various components. Numerical inversion technique has been used to obtain the solutions in the physical domain. Some special cases of interest are also deduced.

네크라인과 헤어스타일이 얼굴 이미지 및 형태 지각에 미치는 영향 (Effect of Neckline-Hairstyle Combinations on the Perception of Face Image and Type)

  • 이영미;서미아
    • 복식문화연구
    • /
    • 제6권4호
    • /
    • pp.13-25
    • /
    • 1998
  • This study focuses on the visual effects of various neckline-haristyle combinations on the perception of face image and type. The study employed a model with oval face and examined 35 combinations made up of five necklines and seven hairstyles. Looking at various face images depending upon different necklines, in case of round, V, boat square, and high necklines, long wave hair and medium wave hair produced a feminine image of marked individuality; long straight hair that covers the forehead and medium straight hair gave an image of charm and purity as well as an image of neatness; and long straight hair short cut hair showed an intellectual image. Regarding the perceptual type of face depending upon the different necklines of round, V, square, and high, the long straight hair covering the forehead and medium wave hair had the effect of an optical illusion that made the face look short and round; and short cut hair made the face line look distinct and the face look oval and slender.

  • PDF

중소기업의 혁신활동과 기법이 혁신성과에 미치는 영향에 관한 연구 (A Study on the Effect of Innovation Performance Through Business Innovation Activities and Techniques for the Small & Medium Size Company)

  • 문희영;장석주
    • 품질경영학회지
    • /
    • 제43권2호
    • /
    • pp.151-168
    • /
    • 2015
  • Purpose: The purpose of this study was to investigate the effect of the innovation performance through business innovation activities by small and medium size company as well as to test the moderating effects on the innovation techniques'understanding and conformance. Methods: The data for the empirical analysis was collected from 132 companies mainly located in Jeolla and Gyeonggi Province. Cronbach's alpha factor analysis, multiple regression analysis and hierarchical multiple regression were used for statistical methods. Results: The Statistical analysis results show that innovation performance through business innovation techniques and activities was effected by CEO leadership, company capability and training for employees. Conclusion: Through this study we tried to give some suggestions for improving innovation performance through business innovation activities for the small and medium size company.

작약의 화분배양에 의한 캘러스 및 배발생 (Callus Induction and Embryogenesis Through Pollen Culture in Paeonia albiflora PALL)

  • 김영숙;이병기
    • 식물조직배양학회지
    • /
    • 제22권1호
    • /
    • pp.13-17
    • /
    • 1995
  • 작약의 화분배양에 의한 반수체 식물을 유기시킬 목적으로 화분배양을 실시하여 배양중 소포자의 변화를 관찰하고 캘러스 및 배발생에 미치는 저온전처리(5$^{\circ}C$/10 days)의 효과와 배양화분의 생존율을 조사하였다. 작약의 화분배양시 캘러스 및 배발생에는 저온전처리의 효과가 다소 인정되었으나 약배양의 효과에는 미치지 못하였다. 화분를 첨가한 고체배지에 이식했을 때 구형배가 형성되었다. 화분의 생존율에는 저온전처리 및 배지조성간 의 영향이 미치지 않았으며 배양기간이 지남에 따라 화분의 생존율도 저하되었다.

  • PDF

Acoustic Characteristics of Sand Sediment Slab with Water- and Air-filled Pore

  • Roh Heui-Seol;Lee Kang Il;Yoon Suk Wang
    • 한국음향학회:학술대회논문집
    • /
    • 한국음향학회 2001년도 추계학술발표대회 논문집 제20권 2호
    • /
    • pp.223-226
    • /
    • 2001
  • Acoustic pressure transmission coefficient and phase velocity are measured as the functions of water porosity and air porosity in sand sediment slabs with water- and air-filled pores. Pores in the sand sediment slab we modeled as the structure of circular cylindrical tube shape filled with water and air. The first kind(fast) wave and second kind (slow) wave, identified by Biot, in the solid and fluid mixed medium are affected by the presence of water and air pores. Acoustic characteristics of such porous medium in water are also theoretically investigated in terms of the modified Biot-Attenborough (MBA) model, which uses the separate treatment of viscosity effect and thermal effect in non-rigid porous medium with water- and air-filed pores. The information on the fast waves introduces new concepts of the generalized tortuosity factor and dynamic shape factor.

  • PDF

연초의 근권환경이 뿌리와 지상부의 생육에 미치는 영향 (The effect of root zone environment on the growth of shoot and root of tobacco plant)

  • 이부경
    • 한국연초학회지
    • /
    • 제11권2호
    • /
    • pp.197-202
    • /
    • 1989
  • This experiment was conducted to investigate the effect of growth medium in pots composed of upland soil(S), rice straw manure(M), carbonated rice hull(CRH), and their mixture on growth of tobacco cv. NC 82. The growth of shoot and root was vigorous in order of medium S+M+CRH>M>CRH>S. In M+S medium, root growth in the part of manure was superior to upland soil. But root growth of upland soil part in M+S plot was more vigorous than that in upland soil only. It is possibly due to be influenced by manure in M+S plot. Total length and weight of root, number of roots, and especially for development of adventitious root were closely related to shoot growth. Roots grown in upland soil part was brownish gray in color, while the roots in manure part was milky white. The milky white colored roots had longer life than others. It was concluded that root zone environments derived from several media in pots closely related to shoot growth and disease tolerance of tobacco plant.

  • PDF

Effect of Carboplatin in Combination with Hyperthermia on Cell Death in Human Retinoblastoma Cell Lines

  • Park, H.J.;Park, S.R.;Park, E.K.;Chung, H.S.;H.E.Ahn;Y.H.Rhee;Ha, S.W.;Lim, B.U.
    • 한국생물물리학회:학술대회논문집
    • /
    • 한국생물물리학회 2001년도 학술 발표회 진행표 및 논문초록
    • /
    • pp.60-60
    • /
    • 2001
  • We have investigated the effect of environmental acidity on the induction of apoptosis caused by heat and carboplatin alone or combined. The degree of apoptosis after heating at 42.5$^{\circ}C$ for 1h in pH 6.6 medium was greater than that in pH 7.5 medium in WERI human retinobalstoma cells. When heated in the same pH medium, more apoptosis occurred in the WERI cells than in the Y79 human retinoblastoma cells.(omitted)

  • PDF