• Title/Summary/Keyword: Medium composition

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Effect of Initial pH and L-Arginine on the Composition of Fatty Acids of Streptomyces viridochromogenes

  • Oh, Choong Hun;Jung, Sang Oun;Pyee Jae Ho;Kim Jae Heon
    • Journal of Microbiology
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    • v.34 no.4
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    • pp.316-319
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    • 1996
  • Mycelia of Streptomyces viridochromogenes grown under different pH were analysed for the fatty acid composition. The low relative proportion of 12-methyltetradecanoic acid and the high relative proportion of palmitic acid were characteristic for the young culture under slight acidic pH that caused delay of the aerial mycelium formation. The addition of L-arginine to the culture medium enabled an arginine auxotroph with bald phenotype to have the fatty acid composition similar to that of the wild type and to develop aerial mycelium. The ratio of 12-methyltetradecanoic acid to palmitic acid might be used as a parameter to explain the optimum grwoth in the respect of membrane fluidity.

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Chemical Analysis of Woody Resource Seperated from Municipal Soild Waste (도시 고체 폐기물에서 분리한 목질계 자원의 화학적 분석)

  • Shin, Soo-Jeong;Park, Jong-Moon;Choi, Tea-Ho;Kim, Byung-Ro;Cho, Dae-Haeng;Kim, Yong-Hwan
    • Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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    • 2011.10a
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    • pp.43-47
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    • 2011
  • In woody waste separated from municipal solid waste, medium density fiberboard was major contributors with particleboard, paper, plywood and log, with different composition based on collected period. In chemical compositional analysis of woody waste, it was similar to softwood based on carbohydrate composition analysis. Based on the carbohydrate composition, saccharified solution from MWW could be good resource for biorefinery.

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TOF-MEIS System을 이용한 Ultra Thin Film 및 Composition and the Core/Shell Structure of Quantum Dot 분석

  • Jeong, Gang-Won;Kim, Jae-Yeong;Mun, Dae-Won
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.284-284
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    • 2013
  • 중 에너지 이온 산란 분석법(Medium Energy Ion Scattering Spectrometer, MEIS)은 50~500 keV로 이온을 가속 후 시료에 입사시켜 시료의 원자와 핵간 충돌로 산란되는 일차이온의 에너지를 측정하여 시료를 분석하는 기법으로, 원자층의 깊이 분해능으로 초박막의 표면 계면의 조성과 구조를 분석 할수 있는 유용한 미세 분석기술이다. 본 실험에서 에너지 70~100 keV의 He+ 이온을 사용하여 Pulse Width 1 ns의 Pulsed ion beam을 만들어 Start 신호로 사용하고 Delay-line-detector에 검출된 신호를 End 신호를 이용한 TOF-MEIS System을 개발하였다. 활용 가능한 분석시편으로 Ultra thin film 시편으로 1, 1.5, 2, 2.5, 3, 4 nm의 HfO2, 1.8, 4nm의 SiO2 시편을 분석 하였으며 Ultra Shallow Junction 시편으로 As Doped Si, Cs Doped Si 시편 및 Composition, Core/shell 구조의 Q-dot 시편으로 CdSe, CdSe/ZnS등 다양한 분석 실험을 진행 하였다. Composition, Core/shell 구조의 Q-dot 시편은 Diamond Like Carbon(DLC)의 Substrate에 Mono-layer로 형성하여 분석하였다.

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Effect of Bentonite Type on Thermal Conductivity in a HLW Repository

  • Lee, Gi-Jun;Yoon, Seok;Cho, Won-Jin
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.19 no.3
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    • pp.331-338
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    • 2021
  • Extensive studies have been conducted on thermal conductivity of bentonite buffer materials, as it affects the safety performance of barriers engineered to contain high-level radioactive waste. Bentonite is composed of several minerals, and studies have shown that the difference in the thermal conductivity of bentonites is due to the variation in their mineral composition. However, the specific reasons contributing to the difference, especially with regard to the thermal conductivity of bentonites with similar mineral composition, have not been elucidated. Therefore, in this study, bentonites with significantly different thermal conductivities, but of similar mineral compositions, are investigated. Most bentonites contain more than 60% of montmorillonite. Therefore, it is believed that the exchangeable cations of montmorillonite could affect the thermal conductivity of bentonites. The effect of bentonite type was comparatively analyzed and was verified through the effective medium model for thermal conductivity. Our results show that Ca-type bentonites have a higher thermal conductivity than Na-type bentonites.

Optimal Culturing and Enhancement of Lipid Accumulation in a Microalga Botryococcus braunii (미세조류 Botryococcus braunii의 배양조건 최적화 및 지질축적 향상)

  • Kwon, Sung-Hyun;Lee, Eun-Mi;Cho, Dae-Chul
    • Journal of Environmental Science International
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    • v.21 no.7
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    • pp.779-785
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    • 2012
  • Several tests and experimental work have been done for identifying the best growth conditions and accumulated amount of lipid moiety in B. braunii, a microalga(UTEX 572) in terms of media composition. The specific growth rate was found to be the highest at 0.15 g/L-day when the phosphorus concentration was doubled with the other ingredients at the normal level. Experiments for varied media compositions revealed that the accumulation of lipid was the highest at 48% (dry cell weight based) in the nitrogen deficient medium and its corresponding specific growth rate was comparative to that in the normal BG 11 medium. In the bubble column experiments, carbon dioxide containing air produced four times more cell mass than air only. Light and glucose addition also enhanced cell mass with maximum, 1.8 g/L and accordingly 42% of lipid composition, which turned out to be a better strategy for higher lipid-producing microalgal culture.

Distribution and Composition of Plastic Pellets and Trashon the Beaches of the Southem Coast at Korea (우리나라 남해안 해수욕장의 미소플라스틱 분포와 조성)

  • KIM, Sam-kon;KIM, Jong-Hwa;PARK, Chang-Doo
    • Journal of Fisheries and Marine Sciences Education
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    • v.14 no.1
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    • pp.43-56
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    • 2002
  • (1) This study was carried out to obtain seasonal fluctuations of marine debris in 5 beaches near the Southern Coast of Korea during May, 1999 - April, 2000. The results are as follows: 1. The density of marine debris was remarkably highest at Namhae beach among 5 beaches. The major part of debris in the beach was styrofoam, with the density of 348.5${ea/km}^2$. But, it was Songjung where the largest amount of resin pellets existed. 2. The composition of styrofoam consisted of 51% of the marine debris, wood& papers was 11%, resin pellets & plastics were 8% and others were 16%. 3. Seasonal fluctuations irregularly change in each beach, but the amount of resin pellets were found to be large only in September. It is estimated that resin pellets are beached upon the coastal beaches by the strong warm currents(Tushima currents) during the summer season. 4. Debris fabrication materials that were found had most of parts made up of PE 71.2%, PP 21.2% and EVA 7.7% through these surveys. 5. In the polluted state, the medium one among the 3 specifications(o1d, medium and new state) occupied 88.5% or so.

Isolation of the Exopolysaccharide Producing Microorganism and their Cultural Characterisitics (다당류를 생산하는 미생물의 분리와 배양특성)

  • Son, Bong-Soo;Park, Seok-Kyu;Kang, Shin-Kwon;Lee, Sang-Won;Seong, Chi-Nam;Sung, Nack-Kie
    • Microbiology and Biotechnology Letters
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    • v.23 no.3
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    • pp.263-268
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    • 1995
  • A screening was performed to isolate exoploysaccharide-producing microorganisms, which synthesized specific exopolysaccharide for the substitutive of commercial polysaccharides, from natural sources. Soil bacterium, one of 378 mucoid isolates, was finally selected as potential producer of polysaccharides which made the culture broth very viscous and thus examined in detail for optimal medium composition. Isolated strain was identified as Xanthomonas sp. EPS- 1 from the results of morphological and biochemical characteristics. The composition of optimal medium for exopolysaccharide production was as follows: 50 g sucrose, 1.5 g peptone, 2 g KH$_{2}$PO$_{4}$, 2 g MgSO$_{4}$, -7H$_{2}$O, 3 g NaCl, 0.05 g CaCO$_{3}$, 0.07 g FeSO$_{4}$-7H$_{2}$O and 0.05 g MnSO$_{4}$-7H$_{2}$O in 1 liter of distilled water. From the experiments of temperature and pH dependence, the optimal conditions for exopolysaccharide biosynthesis seemed to be 30$\circ$C and 8.0, respectively. About 14.9 gram of maximum exopolysaccharide per liter was obtained at the initial pH 8.0, 30$\circ$C and 250 rpm in a flask culture. The exopolysaccharide EPS-1 had such potential as an emulsifying agent and a gelling agent in comparision with commercial exopolysaccharide.

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Submerged Culture of Phellinus linteus for Mass Production of Polysaccharides

  • Lee, June-Woo;Baek, Seong-Jin;Kim, Yong-Seok
    • Mycobiology
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    • v.36 no.3
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    • pp.178-182
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    • 2008
  • In order to increase the mycelial production of Phellinus linteus, which exhibits potent anticancer activity, some ingredients of the medium used to culture P. linteus were investigated. The optimal medium composition for the production of Phellinus linteus was determined to be as follows: fructose, 40 g/l; yeast extract, 20 g/l; $K_2HPO_4$, 0.46 g/l; $K_2HPO_4$, 1.00 g/l; M$MgSO_4\cdot7H_2SO$, 0.50 g/l; $FeCl_2\cdot6_2O$, 0.01 g/l; $MnCl_2\cdot4H_2O$, 0.036 g/l; $ZnCl_2$, 0.03 g/l; and $SuSO_4\cdot7H_2O$, 0.005 g/l. The optimal culture conditions were determined to be as follows: temperature, 28$^{\circ}C$; initial pH, 5.5; aeration, 0.6 vvm; and agitation, 100 rpm, respectively. Under optimal composition and conditions, the maximum mycelial biomass achieved in a 5 l jar fermentor was 29.9 g/l.

Fabrication and Evaluation of Powders Containing Calcium Silicate for Solid Self-emulsifying System of Oil (오일 성분의 고형 자가 유화 시스템을 위한 규산칼슘 함유 분말의 제조 및 평가)

  • Sung Giu, Jin
    • Journal of Powder Materials
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    • v.29 no.6
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    • pp.499-504
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    • 2022
  • The objective of this study is to assess the impact of spray drying conditions on medium-chain triglyceride (MCT) loading, solubility, and release of an MCT-loaded solid self-emulsifying system in a water-insoluble oily substance. MCT-loaded solid self-emulsifying systems are prepared by spray drying with SDS and calcium silicate. The effects of inlet temperature (60, 80, or 100℃) and feed solution composition (0, 10, 50, 90, or 100% ethanol) on physicochemical properties of MCT-loaded solid self-emulsifying systems are studied. The inlet temperature significantly affects the water solubility of MCT. Moreover, the feed solution composition significantly affects water solubility, release rate, and MCT loading. The MCT-loaded solid self-emulsifying system obtained at 60℃ using 90% ethanol feed solution shows the best physicochemical properties among the synthesized products and exhibits better water solubility (4.43 ± 0.44 vs. 0 ㎍/mL) and release (94.4 ± 1.6 vs. 32.8 ± 7.4%, 60 min) than a commercial product. Furthermore, the MCT-loaded solid self-emulsifying system shows an excellent emulsion droplet size (approximately 230 nm).

Medium Composition Affecting In Vitro Masspropagation and Morphogenesis in Prothalli of Pteris cretica 'Wilsonii' (Pteris cretica 'Wilsonii'의 전엽체 기내 대량번식 및 형태형성에 미치는 배지 구성물질 및 배양 방법)

  • Shin, So Lim;Hwang, Ju Kwang;Lee, Cheol Hee
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.2
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    • pp.114-120
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    • 2009
  • The most effective conditions of in vitro culture were studied for mass propagation of Pteris cretica 'Wilsonii'. Spores of the species germinated within 7 weeks. The greatest proliferation was obtained with Knop and Hyponex media, but growth was more effective in Hyponex medium. MS medium induced necrosis of prothalli in all strength of nitrogen and sucrose except in case of 0% sucrose. Hyponex medium supplemented with 1% sucrose and 0.6% agar promoted propagation and growth of prothalli. In Hyponex medium, optimal inoculation method was homogenization, but in MS medium dividing colonies of prothalli was more effective. Culturing on solid medium was more effective than liquid culture method. Liquid culture induced necrosis of prothalli. Shaking cultured prothalli showed good growth, but propagation was inhibited compared to those cultured on solid medium.