• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2001.04a
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• pp.66-69
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• 2001

A subsurface containment system which is constructed by pumping a gelling liquid (Colloidal Silica) into the unsaturated medium is investigated by developing a mathematical model and conducting numerical simulations. The proposed model is verified by comparing experimentally and numerically determined hydraulic conductivities of gel-treated soil columns at different Colloidal Silica (CS) injection volumes. The numerical experiments indicate that an impermeable gel layer is formed within the time period twice the gel-point. At the Same normalized time, the CS solutions with lower NaCl concentrations result ill further migration and poor Performance in plugging the pore space.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.03a
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• pp.791-800
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• 2010

This paper presents the construction status and the conceptual designs of midium and high level radioactive waste disposal facilities from all around world. For the midium radioactive waste, a shallow disposal using trench or a deep depth disposal are adopted. However, these are rather focusing on the social and cultural point of view than the technical. Meanwhile, the high level radioactive waste is basically disposed in the deep underground. The corresponding ground conditions are usually dense and composed of sedimentary and crystalline rocks mainly with low permeability. A barrier system is made of canister which consists of copper, titanium, and tin. The inner and outer side of the canister are composed of different materials respectively.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2003.09a
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• pp.391-394
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• 2003

The applicability of in situ microbial filter or biobarrier technology for the remediation of soil and groundwater contaminated with chlorinated solvents was investigated. The efficiency and rates of reductive dechlorination of chlorinated solvents are known to be highly dependent on hydrogen concentration. In this study, the effect of electron donors on the reductive dechlorination of PCE was investigated using vermicompost (worm casting) and peat as permeable reactive barrier medium The effect of organic acids (lactate, butyrate and benzoate), yeast extract and vitamin $B_{l2}$ on the reductive dechlorination was investigated. Compared to the control (no electron donor added), addition of electron donors stimulated the dechlorinated rate. Among the electron donor treatments, lactate/benzoate amendment exhibited the highest dechlorination rate. Since vermicompost and peat are inexpensive and biodegradable and have high sorption capacity, they could be successfully used as biobarrier media, especially when electron donors (for example, lactate/benzoate) are added.d.

• Proceedings of the Korean Environmental Health Society Conference
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• 2001.11a
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• pp.11-16
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• 2001

If microorganisms are injected into porous medium such as soils along with appropriate substrate and nutrients, soil pore size and shape are changed from the initial condition as a result of biofilm formation, which make hydraulic conductivity reduced. In this research, hydraulic conductivity reduction was measured after specific bacterium or fungus was inoculated into soil pore. Hydraulic conductivity was decreased to 10 % ∼ 1 % and maintained constant while substrate was provided. Under the adverse conditions such as no substrate, chemical solution permeation, and freeze-thaw cycles, hydraulic conductivity was increased 30∼50%. Hydraulic conductivity decrease of fungus-soil mixture was faster than that of bacterium-soil mixture. Fungus-soil mixture, however, was more sensitive to the adverse conditions.

• Computational Structural Engineering
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• v.7 no.4
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• pp.127-136
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• 1994

For two-and three-dimensional problems, the vibration isolation effect of barriers in the travel path of waves has been studied using a boundary element method in the frequency domain. The soil is modelled as a half-space or a layered medium. The results indicate that the effectiveness of a wave barrier is strongly dependent on its location and depth with respect to the vibration source and that the optimum values of these two parameters are sensitive to vibration frequencies.

• Journal of Periodontal and Implant Science
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• v.29 no.3
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• pp.483-509
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• 1999

Biodegradable barrier membrane has been demonstrated to have guided bone regeneration capacity on the animal study. The purpose of this study is to evaluate the effects of cultured calvarial cell inoculated on the biodegradable barrier membrane for the regeneration of the artificial bone defect. In this experiment 35 Sprague-Dawley male rats(mean BW 150gm) were used. 30 rats were divided into 3 groups. In group I, defects were covered periosteum without membrane. In group II, defects were repaired using biodegradable barrier membrane. In group III, the defects were repaired using biodegradable barrier membrane seeded with cultured calvarial cell. Every surgical procedure were performed under the general anesthesia by using with intravenous injection of Pentobarbital sodium(30mg/Kg). After anesthesia, 5 rats were sacrificed by decapitation to obtain the calvaria for bone cell culture. Calvarial cells were cultured with Dulbecco's Modified Essential Medium contained with 10% Fetal Bovine Serum under the conventional conditions. The number of cell inoculated on the membrane were $1{\times}10^6$ Cells/ml. The membrane were inserted on the artificial bone defect after 3 days of culture. A single 3-mm diameter full-thickness artificial calvarial defect was made in each animal by using with bone trephine drill. After the every surgical intervention of animal, all of the animals were sacrificed at 1, 2, 3 weeks after surgery by using of perfusion technique. For obtaining histological section, tissues were fixed in 2.5% Glutaraldehyde (0.1M cacodylate buffer, pH 7.2) and Karnovsky's fixative solution, and decalcified with 0.1M disodium ethylene diaminetetraacetate for 3 weeks. Tissue embeding was performed in paraffin and cut parallel to the surface of calvaria. Section in 7${\mu}m$ thickness of tissue was done and stained with Hematoxylin-Eosin. All the specimens were observed under the light microscopy. The following results were obtained. 1 . During the whole period of experiment, fibrous connective tissue was revealed at 1week after surgery which meant rapid soft tissue recovery. The healing rate of defected area into new bone formation of the test group was observed more rapid tendency than other two groups. 2 . The sequence of healing rate of bone defected area was as follows ； test group, positive control, negative control group. 3 . During the experiment, an osteoclastic cell around preexisted bone was not found. New bone formation was originated from the periphery of the remaing bone wall, and gradually extended into central portion of the bone defect. 4 . The biodegradable barrier membrane was observed favorable biocompatibility during this experimental period without any other noticeable foreign body reaction. And mineralization in the newly formed osteoid tissue revealed relatively more rapid than other group since early stage of the healing process. Conclusively, the cultured bone cell inoculated onto the biodegradable barrier membrane may have an important role of regeneration of artificial bone defects of alveolar bone. This study thus demonstrates a tissue-engineering the approach to the repair of bone defects, which may have clinical applications in clinical fields of the dentistry including periodontics.

• Nutrition Research and Practice
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• v.5 no.5
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• pp.396-403
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• 2011

Ceramides (Cer) comprise the major constituent of sphingolipids in the epidermis and are known to play diverse roles in the outermost layers of the skin including water retention and provision of a physical barrier. In addition, they can be hydrolyzed into free sphingoid bases such as $C_{18}$ sphingosine (SO) and $C_{18}$ sphinganine (SA) or can be further metabolized to $C_{18}$ So-1-phosphate (S1P) and $C_{18}$ Sa-1-phosphate (Sa1P) in keratinocytes. The significance of ceramide metabolites emerged from studies reporting altered levels of SO and SA in skin disorders and the role of S1P and Sa1P as signaling lipids. However, the overall metabolism of sphingoid bases and their phosphates during keratinocyte differentiation remains not fully understood. Therefore, in this study, we analyzed these Cer metabolites in the process of keratinocyte differentiation. Three distinct keratinocyte differentiation stages were prepared using 0.07 mM calcium (Ca$^{2+}$) (proliferation stage), 1.2 mM Ca$^{2+}$ (early differentiation stage) in serum-free medium, or serum-containing medium with vitamin C (50 ${\mu}L$/mL) (late differentiation stage). Serum-containing medium was also used to determine whether vitamin C increases the concentrations of sphingoid bases and their phosphates. The production of sphingoid bases and their phosphates after hydrolysis by alkaline phosphatase was determined using high-performance liquid chromatography. Compared to cells treated with 0.07 mM Ca$^{2+}$, levels of SO, SA, S1P, and SA1P were not altered after treatment with 1.2 mM Ca$^{2+}$. However, in keratinocytes cultured in serum-containing medium with vitamin C, levels of SO, SA, S1P, and SA1P were dramatically higher than those in 0.07- and l.2-mM Ca$^{2+}$-treated cells; however, compared to serum-containing medium alone, vitamin C did not significantly enhance their production. Taken together, we demonstrate that late differentiation induced by vitamin C and serum was accompanied by dramatic increases in the concentration of sphingoid bases and their phosphates, although vitamin C alone had no effect on their production.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.13 no.1
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• pp.105-117
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• 2018

In this study, we identify the obstacles that occur through the relationship between I-U cooperation and look for factors that can overcome them in the 'university administration's efforts' and 'Trust between I-U'. In the study of existing I-U cooperation, the relationship between industry and university has accumulated experiences and various channels of bilateral cooperation by sustaining interactions and absorbing capacity of knowledge by path dependence. However, as cooperation increases, 'I-U cooperation barrier' are inevitable, which is explained by two perspectives: 'Difference in mutual recognition' and 'Institutional barriers'. In order to induce the achievement of effective I-U cooperation, it is necessary to overcome these obstacles stemming from mutual relations, and it will be possible to maintain the relationship of continuous I-U cooperation. The researchers conducted research on companies participating in the I-U cooperation technology development project of the 'Ministry of Small and Medium Venture Business', which is a representative I-U cooperation program in Korea. This project will be promoted in the 'Small & Medium Business I-U cooperation Center', an administration-dedicated organization of the university. The researchers measure 'University administration's efforts' and 'Trust between I-U'to overcome'I-U cooperation barrier' In order to clarify the data of the research sample, a questionnaire survey of organizational units was conducted for all companies participating in the 'I-U cooperation technology development projects' of the SMEs and Startups between 2011 and 2015, and the responses of 356 organizations were drawn. The results showed that the higher the level of 'University administration's efforts' and Trust between I-U', the lower 'Difference in mutual recognition' and 'Institutional barriers'. Particularly, it showed higher explanatory power to overcome 'Institutional barriers' among obstacles. Therefore, it should be accompanied by the interest, implementation and institutional support of I-U-R subjects to raise the level of these two factors that can overcome 'I-U cooperation barrier'.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.2
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• pp.123-129
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• 2005

In situ permeable reactive barrier (PRB) technologies have been proposed to reductively remove organic contaminants from the subsurface environment. The major reactive material, zero valent iron ($Fe^0$), is oxidized to ferrous iron or ferric iron in the barriers, resulting in the decreased reactivity. Iron-reducing bacteria can reduce ferric iron to ferrous iron and iron reduced by these bacteria can be applied to dechlorinate chlorinated organic contaminants. Iron reduction by iron reducing bacteria, Shewanella algae BrY, was observed both in aqueous and solid phase and the enhancement of TCE removal by reduced iron was examined in this study. S. algae BrY preferentially reduced Fe(III) in ferric citrate medium and secondly used Fe(III) on the surface of iron oxides as an electron acceptor. Reduced iron formed reactive materials such as green rust ferrihydrite, and biochemical precipitation. These reactive materials formed by the bacteria can enhance TCE removal rate and removal capacity of the reactive barrier in the field.

• Journal of Soil and Groundwater Environment
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• v.11 no.2
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• pp.22-37
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• 2006

The applicability of biobarrier or in situ microbial filter technology for the remediation of groundwater contaminated with chlorinated solvent was investigated through batch microcosm study. The efficiency and rates of reductive dechlorination of tetrachloroethylene (PCE) are known to be highly dependent on hydrogen concentration. In this study, the effect of electron donors on the reductive dechlorination of PCE was investigated using vermicompost (or worm casting) and peat as a biobarrier medium. The effect of organic acids (lactate, butyrate and benzoate), yeast extract and vitamin $B_{12}$ on the reductive dechlorination was investigated. In the absence of biobarrier medium (adsorbent), addition of electron donors stimulated the dechlorination rate of PCE compared to the control experiment (i.e., no electron donor added). Among the treatments, addition of lactate or lactate/benzoate as hydrogen donor exhibited the highest dechlorination rate ($k_1=0.0260{\sim}0.0266\;day^{-1}$). In case of using vermicompost as a biobarrier medium, amendment of lactate/benzoate exhibited the highest dechlorination rate following with a pseudo-first-order degradation rate constant of $k_1=0.0849\;day^{-1}$. In contrast, when Pahokee peat was used as a biobarrier medium, either butyrate or lactate addition exhibited the highest dechlorination rate with $k_1$ values of 0.1092 and $0.1067\;day^{-1}$, respectively. The results of this study showed the potential applicability of in situ biobarrier technology using vermicompost or peat as a barrier material for the remediation of groundwater contaminated with chlorinated solvent.