• 동의생리병리학회지
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• 제19권5호
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• pp.1195-1199
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• 2005

In the present study, Inhibitory effects of the ethanol extract of Saussurea lappa (S. lappa) on the growth, acid production, adhesion and water-insoluble glucan synthesis of Streptouccus mutans (S. mutans) were examined. The growth and acid production of S. mutans were Inhibited by the presence of ethanol extract of S. lappa (0.5-4 mg/ml) significantly. The ethanol extract of S. lappa (0.25-4 mg/ml) also significantly lowered the adherence of S. mutans in a dose dependent manner. In water-insoluble glucan synthesis assay, 2-4 mg/ml of the ethanol extract of S. lappa significantly inhibited the formation of water-insoluble glucan. These results suggest that S. lappa may inhibit the caries-inducing properties of S. mutans. Further studies are necessary to clarify the active constituents of S. lappa responsible for such biomolecular activities.

• 구강회복응용과학지
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• 제27권1호
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• pp.81-90
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• 2011

resin infiltrant의 초기 우식 병소에 침투를 SEM과 CLSM을 사용하여 관찰하고, resin infiltrant와 3가지 접착제(All-bond 2, Scotchbond Multi-purpose, Clearfil SE bond)의 pH와 점도를 평가하고, 영구치에 resin infiltrant 적용 후 표면 조도와 미세경도의 변화를 측정하였다. 점도(Pa.s)는 resin infiltrant($0.173{\pm}0.012$), All-bond 2($0.754{\pm}0.030$), Scotchbond Multi-purpose($1.46{\pm}0.137$), Clearfil SE bond($1.28{\pm}0.125$)로 측정되었다. pH는 resin infiltrant (4.7), All-bond 2(7.5), Scotchbond Multi-purpose(8.2), Clearfil SE bond(4.67)로 측정되었다. resin infiltrant의 적용은 적용하지 않은 시편과 비교할 때 표면 조도와 미세경도에 유의한 차이를 보이지 않았다.(p>0.05).

• 동의생리병리학회지
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• 제21권5호
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• pp.1148-1153
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• 2007

Streptococcus mutans is considered one of the primary etiologic agents of dental caries. we studied the effect of the ethanol extracts of Tribuli fructus (T. fructus) on the growth, biofilm formation, acid production, adhesion and insoluble glucan synthesis of S. mutans. The ethanol extracts of T. fructus showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.025, 0.05, 0.1, 0.2, 0.3, 0.5 mg/ml compared to the control group. In the biofilm assay, the ethanol extracts of T. fructus inhibited formation of biofilm synthesized by S. mutans at the concentration of 0.05 mg/ml. The extracts markedly inhibited S. mutans adherence to HA treated with saliva, and cell adherence was repressed by more than 50% at the concentration 0.05 mg/ml. On the activity of glucosyltransferase which synthesizes water insoluble glucan form sucrose, ethanol extract of T. fructus showed more than 10% inhibition over the concentration of 0.025 mg/ml. Hence, we conclude that T. fructus might be a candidate of anticaries agent. Further studies are necessary to clarify the active constituents of T. fructus responsible for such biomolecular activities.

• Journal of Korean Dental Science
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• 제3권2호
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• pp.20-25
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• 2010

Aim : This study was conducted to assess the oral health status of deaf and mute children attending special school. Materials and Methods : A cross-sectional descriptive survey was conducted among 137 deaf and mute children with ages ranging from 7 to 18 years. A total of 76 males (55.47%) with mean age of $14.2{\pm}4.5$ and 61 females (44.53%) with mean age of $13.8{\pm}4.2$ years and studying in a school for deaf and mute children in Warora were considered. Data were collected using a standard method recommended by WHO for the oral health survey in 1977. Oral health status was assessed using OHIS, Loe and Sinless, and CPI Index along with DMFT and DMFS Index. Gingival position was considered for measuring attachment loss. Statistical analysis was performed using the SPSS software package (version 17.0). Results : The mean DMFT was found to be $2.53{\pm}1.72$, and mean DMFS, $3.37{\pm}3.16$. The prevalence of dental caries was pegged at 35.32%, with mean OHIS score at $1.49{\pm}0.76$. Overall gingival index among deaf and mute children was $0.81{\pm}1.4$, whereas that for the upper arch and lower arch was $0.92{\pm}0.84$ and $1.19{\pm}0.95$, respectively. The mean score for the CPI Index among deaf and mute children was found to be $0.42{\pm}0.32$. Gingival clinical attachment loss was found to be $0.26{\pm}0.15mm$. Conclusion : These findings suggest that children with hearing disabilities can also have good oral hygiene comparable to normal individuals of the same age group. These results may be attributed to the fact that the study sample was taken from a single school of a private organization with a well-equipped dental setup.

• Journal of Korean Dental Science
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• 제16권1호
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• pp.9-22
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• 2023

Xerostomia is defined as the subjective complaint of dry mouth with or without hyposalivation, which is insufficient salivary secretion from salivary gland. Xerostomia can lead to multiple oral symptoms such as dental caries, halitosis, burning mouth syndrome, and oral candidiasis, which can significantly impact the well-being of patients, especially in geriatric patients who may already have compromised health. Clinical findings of xerostomia include decreased salivary flow and alterations in salivary composition. These changes can lead to various oral health problems such as dental caries, periodontitis, swallowing and speaking difficulties, taste disturbances, halitosis, mucosal diseases, and burning mouth syndrome. Recognizing these clinical manifestations is essential for early diagnosis and appropriate management. Although several reasons and risk factors have been suggested for xerostomia such as aging, chemo-radiation therapy, systemic disease, and Sjögren's syndrome, the polypharmacy is recently highlighted especially in elderly patients. Understanding the etiology and risk factors associated with xerostomia is crucial for effective management. To manage xerostomia patients, a multidisciplinary guideline should be established beyond dental care. Through this literature review, we summarized consideration for diagnostic, therapeutic, nursing essentials for the clinical guideline. By addressing the underlying causes and implementing appropriate treatment strategies, healthcare professionals can improve the quality of life for individuals suffering from xerostomia.

• 동의생리병리학회지
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• 제30권1호
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• pp.27-32
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• 2016

Streptococcus mutans plays a virtal role in trigering dental caries establishment due to its ability to synthesize two significant factors. The two factors are organic acids and glucans. The former demineralized dental enamel and the latter mediates the attachment of bacteria to tooth surface. It is believed that demineralization of dental enamel and attachment of bacteria are the crucial events that indicate and develop dental caries. For this reason, we studied the effect of the ethanol extracts of Radix Pulsatillae on the growth and acid production of S. mutans. Ethanol extracts of the Radix Pulsatillae showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition compared to the control groups (p<0.05). The extracts inhibited S. mutans adherence to hydroxyapatite treated with saliva, and cell adherence was repressed by Radix Pulsatillae. the ethanol extract of Radix Pulsatillae showed remarkable inhibition of glucosyltransferase, which synthesizes water insoluble glucan form sucrose. Phytochemical analysis showed Radix Pulsatillae contained major components such as phenolic compounds, glycosides, steroids, terpenoid, and saponin. These results suggest that Radix Pulsatillae may have anti-cariogenic properties, which may be related with major components such as phenolic compounds, glycosides, steroids, terpenoid, and saponin.

• 동의생리병리학회지
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• 제29권1호
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• pp.27-32
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• 2015

Streptococcus mutans triggers dental caries establishment by two major factors. One is synthesis of organic acids which demineralize dental enamel and the other is synthesis of glucans which mediate the attachment of bacteria to the tooth surface. In the present study, we evaluated the effect of the ethanol extracts of Aconitum koreanum (A. koreanum ) on the growth and acid production of S. mutans. Ethanol extracts of the A. koreanum showed concentration dependent inhibitory activity against the growth and acid production of S. mutans, and produced significant inhibition at the concentration of 0.016 mg/ml compared to the control groups (p<0.05). The extracts inhibited S. mutans adherence to hydroxyapatite treated with saliva, and cell adherence was repressed by 50%, 54% at the concentration of 0.063, 0.125 mg/ml. On the study of activation of glucosyltransferase which synthesizes water insoluble glucan form sucrose, the ethanol extract of A. koreanum showed remarkable inhibition over the concentration of 0.016, 0.031, 0.063 and 0.125 mg/ml (p<0.05). Especially on the concentration of 0.063, 0.125 mg/ml, the extracts suppressed the glucan synthesis by 100%. We analyzed the component of the extracts of A. koreanum. The results showed that the extract of A. koreanum had strong phenolic compound, glycosides and organic acids. These results suggest that A. koreanum may inhibit the caries-inducing properties of S. mutans, and which may be related with strong phenolic compound, glycosides and organic acids.

• 치위생과학회지
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• 제13권1호
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• pp.45-52
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• 2013

본 연구에서는, 구강 내 세균에 대한 항세균제로서의 이용가치를 확인하고, 치아우식 예방 및 치료제로 활용될 수 있는 가능성이 있는 물질을 탐색하고자 64종의 한약재 추출물을 사용하여 치아우식증의 원인이 되는 6개 균주를 대상으로 항균활성을 검색하였다. 1. 구강 내 상주균인 S. oralis, 치아우식증에 가장 큰 영향을 미치는 S. mutans, 치면열구우식증에 연관성이 높은 L. casei와 초기 평활면우식증에 영향을 주는 S. sanguinis에 모두 항균활성을 보인 한약재 추출물은 64종 가운데 황금과 소목이었다. 2. 치아우식증 원인균 중 하나인 S. oralis에 대하여 한약재 추출물의 항균 활성을 알아본 결과 64종 가운데 22개의 한약재가 항균 활성을 보였다. 항균활성이 가장 우수한 한약재 는 소목(40 mm)>감국(27 mm)>황금(19 mm)>지부자(17 mm), 대풍자(17 mm) 순이었다. 그 외에 괴화(14 mm), 오가피(9 mm), 목단피(14 mm), 단삼(10 mm), 비자(10 mm), 고삼(10 mm), 시호(11 mm), 인동(10 mm), 음양곽 A (13 mm), 음양곽 B (11 mm), 대황(13 mm)이 항균 활성을 보였다. 3. 소와열구 및 상아질 우식에 관여하는 L. acidophilus는 64종 한약재 추출물 가운데 소목만이 항균성을 보였다. 치아우식증 원인세균인 S. salivarius는 천마(9 mm), 소목(20 mm)의 생육 저해환의 직경을 측정 할 수 있었다. 4. 결론적으로, 치아우식증의 원인이 되는 6종 균주에 대해 가장 우수한 활성을 보인 한약재는 소목으로 최대 40 mm의 생육 저해환이 측정되었다. 이상의 결과를 볼 때, 한약재 추출물은 구강 내 우식유발균주의 생육을 억제하는 효과가 있어, 향후 구강 내 우식원성 세균의 치료물질로 활용을 제시한다.

• 대한소아치과학회지
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• 제34권1호
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• pp.130-139
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• 2007

다운증후군 환자의 치아우식증과 구강내 여러 요인들과의 연관성을 평가하고자 9-18세의 다운증후군 환자 25명과 정상인 63명을 대상으로 치아우식경험지수, 치태지수를 조사하고 효소면역분석법을 이용하여 타액의 면역항체 IgA와 뮤탄스 연쇄상구균-특이 IgA농도를 측정하고, Dentocult 키트를 이용하여 뮤탄스 연쇄상구균과 유산간균의 수, 타액의 완충능을 측정하여 두 군의 각 값들을 독립 t-test와 카이제곱 검정을 시행하였다. 다운증후군 환자에서 유치우식경험은 정상인에 비해 유의하게 낮았으나(p<0.001), 영구치우식경험은 유의한 차이를 보이지 않았다. 치태지수와 타액의 IgA의 농도는 두 군간에 차이를 보이지 않았으며, 뮤탄스 연쇄상구균 특이-IgA 농도와 타액 완충능은 다운증후군환자에서 낮았다(p<0.05). 뮤탄스 연쇄상구균 수와 유산간균 수는 두 군간에 유의한 차이는 없었다. 9-11세 군에서 뮤탄스 연쇄상구균의 수는 다운증후군 환자에서 정상인보다 낮았으며(p<0.001), 뮤탄스 연쇄상구균 특이-IgA는 다운증후군 환자에서 유의하게 낮았다(p<0.05). 유치의 치아우식경험치면지수는 완충능, 뮤탄스 연쇄상구균의 수와 유의한 상관관계를 보였다.

• 농촌의학ㆍ지역보건
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• 제45권2호
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• pp.79-88
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• 2020

연구목적: 이 연구는 지역사회 치면열구전색 사업의 치아우식 예방효과를 평가하고자 시행되었다. 연구방법: 연구 대상자는 2005년부터 2008년까지 김천시에 소재한 34개 초등학교에 재학 중인 9,001명 중 2005년 최초 구강검사 당시 상·하악 제1대구치에 치아우식이 없는 치아와 학생 4,768명을 최종 대상자로 선정하였다. 2005년도에 치면열구전색을 실시한 1,478명에 대해 3년간 추적조사가 이루어졌고, 2006년에 치면열구전색을 실시한 999명에 대해 2년간 추적조사를 하였으며, 2007년에 치면열구전색을 실시한 458명에 대해 1년간 추적조사 후 기술통계 및 교차분석을 시행하였다. 연구결과: 치면열구전색 실시군의 DMFT rate는 1년 추적 후 1.4%에서 3년 추적 후 4.1%, DMFT index는 1년 추적 후 0.18개에서 3년 추적 후 0.70개로 추적기간이 길어질수록 높게 나타났다. 1일 평균 칫솔질 횟수는 DMFT rate와 DMFT index에 영향을 미쳤다. 즉 치면열구전색을 실시한 대상자라도 1일 평균 칫솔질 횟수가 적으면 DMFT rate와 DMFT index는 높게 나타났다. 결론: 치면열구전색을 실시한 대상자에서도 올바른 칫솔질에 대한 구강보건교육이 선행되어야 한다.