• Title/Summary/Keyword: Medicinal effects

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Method Validation for the Determination of Eleutherosides and β-Glucan in Acanthopanax koreanum (탐라오가피의 Eleutheroside B, E 및 β-Glucan 함량 분석 및 분석법 검증)

  • Kim, Young-Hyun;Bae, Da-Bin;Park, Sun-Ok;Lee, Sang-Jong;Cho, Ok-Hyun;Lee, Ok-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.9
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    • pp.1419-1425
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    • 2013
  • The aim of this study was to investigate the method validation for the determination of eleutherosides (B and E) and ${\beta}$-glucan in Acanthopanax (A.) koreanum. This medicinal plant reportedly mainly included eleutherosides which exhibit the pharmacological effects, and ${\beta}$-glucan substantially enhances the function of the immune system by activating macrophages. The specificity, linearity, precision, accuracy, limit of detection (LOD, S/N=3), and limit of quantification (LOQ, S/N=10) were measured by HPLC and enzymatic methods. Our results showed that the coefficient of calibration correlation ($R^2$) for eleutheroside B and E were 0.9997 and 0.9999, respectively. The limits of detection (LOD) for eleutheroside B and E were $0.050{\mu}g/mL$ and $0.025{\mu}g/mL$, respectively. The recovery rate of eleutheroside B and E were revealed in the high range of 100.66~110.04% and 94.26~111.62%, respectively. The inter-day precision of eleutheroside B and E in the root and stem in A. koreanum were 1.4~5.0% and 1.1~2.5%, respectively. The intra-day precision of eleutheroside B and E in the root and stem in A. koreanum were 2.8~2.9% and 0.4~1.1%, respectively. Furthermore, the inter-day and intra-day precision of ${\beta}$-glucan in the stem, leaf, and fruit of A. koreanum were 1.32~5.67% and 8.01~11.76%, respectively. In conclusion, the methods were validated for the detection of eleutherosides and ${\beta}$-glucan in A. koreanum.

Plant regeneration and soil acclimatization through photoautotrophic culture from leaf explant of a rare species in Sedum tosaense Makino (희귀수종인 주걱비름(Sedum tosaense Makino)의 잎절편으로부터 기내 식물체 재분화 및 광독립배양을 통한 토양순화)

  • Ko, Myoung-Suk;Bae, Kee Hwa;Song, Gwanpil;So, In Sup
    • Journal of Plant Biotechnology
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    • v.40 no.2
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    • pp.79-87
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    • 2013
  • The aim of this study was to establish plant regeneration from leaf explants of Sedum tosaense Makino, which is globally rare and endangered species. The leaf explants of S. tosaense were cultured on the MS medium supplemented with different concentration of BA and NAA for callus induction. Callus induction was showed the highest (100%) on MS medium containing $2.0mg{\cdot}L^{-1}$ BA and $1.0mg{\cdot}L^{-1}$ NAA. The highest number of shoots were regenerated when callus were cultured on MS medium containing $2.0mg{\cdot}L^{-1}$ BA and $1.0mg{\cdot}L^{-1}$ NAA for 5 weeks. The axillary bud were cultured on the MS media supplemented with combination of BA and NAA for in vitro propagation. The highest number of adventitious shoot (7.9 per explants) formed at $1.0mg{\cdot}L^{-1}$ NAA and $2.0mg{\cdot}L^{-1}$ BA. For rooting, MS medium supplemented with or without $2.0g{\cdot}L^{-1}$ activated charcoal was tested. The optimal results were observed using MS medium supplemented with $2.0g{\cdot}L^{-1}$ activated charcoal, on which 85.7 (No. of root), 4.6 cm (length of root). 1,200 ppm $CO_2$ and 350 ppm $CO_2$ were supplied for make certain the effects of $CO_2$ on pre-acclimatization by photoautotrophic culture. 1,200 ppm $CO_2$ treatment was established higher than 350 ppm $CO_2$ treatment. Soil acclimatization of in vitro plantlets was the best in mixture soil consisted of peat moss and perlite with 100% survival rate and they showed the maximum growth.

Studies on the Shelf-life Extension of Jeotkal, Salted and Fermented Seafood (젓갈류의 유통기한 연장을 위한 연구)

  • Cho, Hak-Rae;Park, Uk-Yeon;Chang, Dong-Suck
    • Korean Journal of Food Science and Technology
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    • v.34 no.4
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    • pp.652-660
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    • 2002
  • To develop natural food preservatives for extending the shelf-life of jeotkal (salted and fermented seafood), antimicrobial substances were extracted from 32 types of medicinal herbs and edible plants using 95% ethanol. Among the extracts, Glycyrrhizae radix, Curcumae domestica, Galla rhois, and Resina pini showed relatively high inhibitory effects on the growth of the microorganisms isolated from the deteriorated jeotkal. We selected and tested the extract from Recina pini as a natural jeotkal preservative. This ethanol extract was purified partially by adding equal quantity of water, through which 77% of insoluble materials were removed as impurities. In manufacturing modified jeotkal using squid, sucrose and starch syrup were substituted with sorbitol, $glucono-{\delta}-lactone$ was added instead of vitamin C and lactic acid, and sterilized hot pepper was used instead of natural one. The shelf-life of modified jeotkal was prolonged by 4 days compared with the control jeotkal when stored at $20^{\circ}C$, while that of modified jeotkal containing 1.0% partially purified Recina pini extract was prolonged by 6 days compared to the control. The same tests were conducted for the changran (stomach and intestine of Alaska pollack) jeotkal preservation. The shelf-life of the control jeotkal was 24 days, whereas the modified jeotkal and the Resina pini extract-containing modified jeotkal maintained their qualities without changes in microbial and chemical characteristics for 90 days at $20^{\circ}C$ storage.

Antioxidant Activity of Extracts from Smilax china Root (청미래덩굴(Smilax china) 뿌리 추출물의 항산화활성 효과)

  • Song, Hee-Sun;Park, Yeon-Hee;Jung, Sae-Heung;Kim, Dong-Pil;Jung, Yong-Hee;Lee, Mi-Kyung;Moon, Ki-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.9
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    • pp.1133-1138
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    • 2006
  • Smilax china root has been used as traditional medicinal remedy in China and Korea and reported to have various biological activities such as anti-inflammatory, antimutagenic and antimicrobial activities. In this study, the possibility of development as natural antioxidants of Smilax china root extracts was investigated. For the evaluation of antioxidant activity, aqueous- and 25% EtOH extract from Smilax china root were prepared and six different evaluation assay methods, i.e., measurement of total phenolics, radical scavenging effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO) and nitrite $(NO_2)$, reducing power, and inhibitory effect on tyrosinase activity, were used. The total phenolics content of two extracts was high as the level of 36 mg of gallic acid equivalent per 1 g of dried sample tested. The radical scavenging activities of ethanol extract toward DPPH and NO were better than those of aqueous extract (p<0.05). The $NO_2$ scavenging activity of both extracts showed the highest value at pH 1.2 (98%). Especially, the $NO_2$ scavenging activities of EtOH extract were significantly stronger than those of aqueous one at pH 4.2 (51%) and pH 6.0 (32%), respectively. In the reducing power test, both extracts revealed higher ferric ion reducing activity than known antioxidant, vitamin C at the level of $0.05\sim0.1mg/mL$ (p<0.01). The 1 mL of aqueous and 25% EtOH extract showed effective inhibition activity on tyrosinase activity as 45% and 53%, respectively. Therefore, these results suggest that two extracts from Smilax china root may serve as useful natural antioxidants.

Development of New Natural Antioxidants for Cosmeceuticals (천연물 유래 항산화 기능성 화장품 신소재 개발)

  • Yoo, Ick-Dong;Kim, Jong-Pyung;Kim, Won-Gon;Yun, Bong-Sik;Ryoo, In-Ja
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.31 no.4 s.54
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    • pp.349-357
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    • 2005
  • New antioxidative substances for cosmeceuticals were screened from natural resources such as microbial metabolites, mushrooms, and medicinal plants. Four antioxidants were isolated from the fungal metabolite of Eupenicillium shearii and their structures were determined to be new phenolic compounds. The compounds were designated as melanocins A, B, C, and D. Melanocins $A{\sim}D$ exhibited free radical scavenging activity on DPPH and superoxide with $EC_{50}$ values of $21{\sim}94\;and\;7{\sim}84{\mu}M$, respectively, which were stronger activity than those of ${\alpha}-tocopherol$ and BHA. Melanocin A showed anti-wrinkle effects on the UV-irrated hairless mouse skin. A novel hispidin antioxidative compound designated as inoscavin A was isolated from the fruiting body of the mushroom, Inonotus xeranticus. Inoscavin A scavenged superoxide radical with $EC_{50}$ values of $0.03{\mu}g/mL$, and inhibited rat liver microsomal lipid peroxidation with $EC_{50}$ values of $0.3{\mu}g/mL$. Benzastatins $A{\sim}G$, the novel antioxidants isolated from the culture of Streptomyces nitrosporeus showed potent lipid peroxidation inhibitory activity with $EC_{50}$ values of $3{\sim}30{\mu}M$. A cyclopentene compound with strong hypopigmentary effect was isolated from the fungal metabolite of Penicillium sp. and identifed as terrein. Terrein significantly reduced melanin levels in a melanomacyte cell line, Mel-Ab. It showed 10 times stronger activity than kojic acid, but exhibited no cytotoxic effect even in $100{\mu}M$. It was suggested that terrein reduced melanin synthesis by reducing tyrosinase production by MITF down-regulation.

Isolation and Characterization of Elastase Inhibitor from Areca catechu (빈랑으로부터 Elastase 저해물질의 분리 및 특성조사)

  • 조중제;이건국;조병기;최정도
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.26 no.1
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    • pp.163-186
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    • 2000
  • We have previously screened 150 medicinal plants on the inhibition of elastase and found a significant inhibitory effects of the extracts of Areca catechu L. on the aging and inflammation against the skin tissues. To isolate and identify the compounds having biological activity, we was further purified by each of the solvent fractions, silica gel column chromatography, preparative TLC and reversed-Phase HPLC. Peak in HPLC, which coincided with the inhibitory activity against elastase, was identified as Phenolic substance using various colorimetric methods, UV, and IR. $IC_{50}$/ values of phenolic substance purified from Areca catechu were 26.9 $\mu\textrm{g}$/$m\ell$ for porcine pancreatic elastase (PPE) and 60.8 $\mu\textrm{g}$/$m\ell$ for human neutrophil elastase (HNE). This Phenolic substance showed more potent activity than those of reference compounds, oleanolic acid (76.5 $\mu\textrm{g}$/$m\ell$ for PPE, 219.2 $\mu\textrm{g}$/$m\ell$ for HNE) and ursolic acid (31.0 $\mu\textrm{g}$/$m\ell$ for PPE, 118.6 $\mu\textrm{g}$/$m\ell$ for HNE). According to the Lineweaver-Burk Plots, the inhibition against both PPE and HNE by this phenolic substance was competitive with substrate. Phenolic substance from Areca catechu exhibited high free radical scavenging effect ($SC_{50}$/ : 6 $\mu\textrm{g}$/$m\ell$) and inhibited effectively hyaluronidase activity ($IC_{50}$/: 210 $\mu\textrm{g}$/$m\ell$). These results suggest that the Phenolic substance Purified from Areca catechu showed anti-aging effect by protecting connective tissue proteins.

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Effects of Selenium(Se) on Growth and Se Content of Mungbean (셀레늄 시용에 따른 녹두 생육 및 Se 함량 변화)

  • Kim, Dong-Kwan;Jung, Byung-Joon;Son, Dong-Mo;Chon, Sang-Uk;Lee, Kyung-Dong;Kim, Kwan-Su;Rim, Yo-Sup
    • Korean Journal of Plant Resources
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    • v.20 no.5
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    • pp.383-388
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    • 2007
  • This study examined the effective treatment method of selenium and translocation characteristics of selenium in order to produce mungbean containing selenium. The foliar application of selenium at 3.5, 7, 14, and $28mg/{\ell}$ during the flowering period, yielded a relatively high record of seeds containing $0.41{\sim}3.96mg/kg$ and $0.27{\sim}2.38mg/kg$ of selenium, from the first and second harvesting. However, seeds from the first harvesting contained $52{\sim}71%$ more selenium than the seeds from the second harvesting. On the other hand, seeds from first and second harvesting of the non-treatment group had the same amount of selenium at 0.02mg/kg only. When the foliar application of selenium at $7mg/{\ell}$ was conducted two or three times, seeds from the first to third harvesting contained just the equal amount of selenium. However, when it was conducted only once, seeds from the first harvesting contained 56% and 67% more than seeds from the second and third harvesting, respectively. In seeds of mungbean containing 2.05mg/kg of selenium, cotyledon had 2.99mg/kg of selenium, which was 38% more than seed coat per unit weight. When mungbean sprout was produced, selenium content was 5.51mg/kg, but seed coats by-product of sprouts had 0.78mg/kg of selenium. The growth and quantity of mungbean was not significantly different according to the concentration and the frequency of foliar application of selenium used for in study.

Changes in the constituents and UV-photoprotective activity of Astragalus membranaceus caused by roasting (황기의 볶음 조건에 따른 성분 및 자외선 광보호 활성 변화)

  • Park, Jeong-Yong;Lee, Ji Yeon;Kim, Hyung Don;Jang, Gwi Yeong;Seo, Kyung Hye
    • Journal of Nutrition and Health
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    • v.52 no.5
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    • pp.413-421
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    • 2019
  • Purpose: Astragalus membranaceus (AM) is an important traditional medicinal herb. Pharmacological research has indicated that AM has various physiological activities such as antioxidant, anti-inflammatory, immunoregulatory, anticancer, hypolipidemic, antihyperglycemic, and hepatoprotective activities. The bioactive substances responsible for the physiological activities in AM, including many antioxidant substances, change during the roasting process. This study investigated and compared the changes in the antioxidant constituents of AM caused by roasting. Methods: DPPH (1,1-diphenyl-2-picryl hydrazyl) and $ABTS^+$ (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging activities and their total phenolic content (TPC) were measured. High-performance liquid chromatography (HPLC) analysis was performed to confirm any changes in the isoflavonoids of roasted AM (R-AM),. The cell viability of UVB-induced HDF (Human dermal fibroblast) cells treated with AM and R-AM extracts was investigated. The comet assay was used to examine the inhibitory effects of R-AM extracts on DNA damage caused by oxidative stress. Results: The DPPH and $ABTS^+$ radical scavenging activities were $564.6{\pm}20.9$ and $108.2{\pm}3.1$ ($IC_{50}$ value) respectively, from the 2R-AM. The total phenol content was $47.80{\pm}1.40mg$ GAE/g from the 1R-AM. The values of calycosin and formononetin, which are the known isoflavonoid constituents of AM, were $778.58{\pm}2.72$ and $726.80{\pm}3.45{\mu}g/g$ respectively, from the 2R-AM. Treatment of the HDF cells with R-AM ($50{\sim}200{\mu}g/mL$) did not affect the cell viability. Furthermore, the R-AM extracts effectively protected against UVB-induced DNA damage. Conclusion: The findings of this study indicate that R-AM increases its isoflavonoid constituents and protects against UVB-induced DNA damage in HDF cells.

Whitening and Antioxidant Effects of Extracts from Angelica gigas Nakai Thin Root (참당귀 세미 추출물의 미백 및 항산화 효과)

  • Lee, Sang-Hoon;Park, Jeong-Yong;Seo, Kyung Hye;Choi, Jang Nam;Lee, Yoon-Jeong;Moon, Youn-Ho;Hur, Mok;Park, Woo Tae;Huh, Yun Chan;Chang, Jae-Ki;Koo, Sung Cheol
    • Korean Journal of Plant Resources
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    • v.34 no.1
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    • pp.37-43
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    • 2021
  • Angelica gigas Nakai (AGN) is a perennial plant belonging to the family Apiaceae. Its root has been utilized as a traditional medicine especially in Korea. This study was carried out to evaluate the potential use of extracts from AGN root parts as a cosmetic material. The dried AGN roots are divided into body (B), thick root (TkR), medium root(MR) and thin root (TnR) according to their diameter before cutting into medicine. B, TkR and MR of AGN are combined and used as medicinal herbs (MH). The extracts from AGN each root part (B, TkR, MR, TnR, MH) were used to test the effect on cell viability using MTS assay and to examine inhibitory effect on melanin accumulation in B16F10 melanoma cells. All extracts (50 - 200 ㎍/mL) from the each root part did not affect the cell viability. And inhibitory effect of all root extracts (200 ㎍/mL) on melanin accumulation was 12-19%. Especially, TnR showed similar inhibitory effect on melanin accumulation to MH. In addition, DPPH and ABTS free radical scavenging activity were higher in the TnR extract compared to MH. This study showed that the TnR extract exhibit high inhibitory effect on melanin accumulation and antioxidant activity compared to MH, suggesting that TnR extract has potential as a cosmetic ingredient.

Effects of Temperature, Light Intensity and Soil Moisture on Growth, Yield and Essential Oil Content in Valerian(Valeriana fauriei var. dasycarpa Hara) (쥐오줌풀의 생육 및 수량과 정유성분에 미치는 온도, 광도, 토양수분의 영향)

  • Cho, Chang-Hwan;Lee, Jong-Chul;Choi, Young-Hyun;Han, Ouk-Kyu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.1
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    • pp.22-32
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    • 1997
  • This experiment was conducted to obtain information for the cultivation of Korean valerian(Valeriana lauriei var. dasycarpa Hara) which will be useful for medicinal and aromatic resources. The effect of different temperature conditions, light intensities and soil water conditions on growth, yield and component of essential oil of V. fauriei were measured at the Dankook University, Cheonan, and a study on the shading treatment was at Umsung, Chungchongbukdo, and Jinbu, Kangwondo, in 1995. V. laudei was planted at five different temperature conditions, 10, 15, 20, 25 and 3$0^{\circ}C$, eight light intensity conditions, 1, 000, 2, 500, 5, 000, 20, 000, 30, 000, 40, 000, 50, 000 and 60, 000lux, six soil water contents, 30, 45, 55, 70, 80 and 90% of the saturated soil, during growth stage. Shading treatment was three conditions, 0, 25 and 50%, during the daytime in field conditions. Photosynthesis had a highly significant relationship with temperature conditions in a quadratic regression model, from which the temperature for the plant growth was estimated to be 17.7$^{\circ}C$. A highly significant quadratic regression was noted between temperature and leaf width or root weight of V. fauriei. It was estimated from the regression equation that the optimum temperature for root growth was 20.3$^{\circ}C$. The content of essential oil and extract rate of root was the highest in the 15~2$0^{\circ}C$. Photosynthesis also was significantly affected by light intensity in a quadratic regression model, from which the optimum light intensity for the growth was estimated to be 40, 000lux. Root yield was more produced in Jinbu than that of in Umsung. The root yield was increased by the shading treatment in Umsung, whereas it was decreased by the shading treatment in Jinbu. The content of essential oil was not affected by the shading treatment of plants during the cultivation, while the compositions of components of essential oil were related to the growing locations. As soil water content was higher, the growth and content of root extract were increased. The optimum soil moisture for the growth of V. fauriei was 80~90% of the saturated soil. In summary, the results indicated that the growth, yield and component of essential oil in V. fauriei were affected by environmental factors as well as soil moisture.

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