• 제목/요약/키워드: Medicinal crop

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이상기온에 따른 2년생 인삼 신품종 및 계통간 조기발뇌 특성 비교 (Comparison of Early Budding Characteristics in New Varieties and Lines 2-Year-Old Ginseng(Panax ginseng C. A. Meyer) by Regional Unusual Temperature in Cheorwon)

  • 정햇님;허수정;김성일;조윤상;안문섭;김영창;김동휘;차선우;성봉재;한승호;김현호
    • 한국약용작물학회:학술대회논문집
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    • 한국약용작물학회 2012년도 심포지엄 및 춘계학술발표회
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    • pp.165-166
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    • 2012

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Thermotolerant Transgenic Ginseng (Panax ginseng C.A. Meyer) by Introducing Isoprene Synthase Gene through Agrobacterium tumefaciens-mediated Transformation

  • Kim, Ok-Tae;Hyun, Dong-Yun;Bang, Kyong-Hwan;Jung, Su-Jin;Kim, Young-Chang;Shin, Yu-Su;Kim, Dong-Hwi;Kim, Swon-Won;Seong, Nak-Sul;Cha, Seon-Woo;Park, Hee-Woon
    • 한국약용작물학회지
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    • 제15권2호
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    • pp.95-99
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    • 2007

  • The cost of conventional cultivation of ginseng (Panax ginseng C.A. Meyer) is very expensive, because shadow condition should be maintained during cultivation periods owing to inherently weak plant for high-temperature. Therefore, application of plant biotechnology may be possible to overcome these difficulties caused by conventional breeding of ginseng. Transgenic plants were produced via Agrobacterium tumefaciens Gv3101, both carrying the binary plasmid pBI121 mLPISO with nptII and Iso (isoprene synthase) gene. Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using nptII primers and Iso primers. RT-PCR result also demonstrated the foreign isoprene synthase gene in three transgenic plant lines (T1, T3, and T5) which was expressed at the transcriptional level. When whole plants of transgenic ginseng were exposed to high temperature at $46^{\circ}C$ for 1 h, a non-transformed plant was wilted from heat shock, whereas a transgenic plant appeared to remain healthy. We suggest that the introduction of exogenous isoprene synthase is considered as alternative methods far generating thermotolerance ginseng.

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Factors Affecting Agrobacterium tumefaciens-mediated Transformation of Panax ginseng C.A. Meyer

  • Kim, Ok-Tae;Jung, Su-Jin;Bang, Kyong-Hwan;Kim, Young-Chang;Shin, Yu-Su;Sung, Jung-Sook;Park, Chun-Geon;Seong, Nak-Sul;Cha, Seon-Woo;Park, Hee-Woon
    • 한국약용작물학회지
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    • 제15권2호
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    • pp.100-104
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    • 2007

  • A protocol for the production of transgenic Panax ginseng C.A. Meyer was established via Agrobacterium tumefaciens-mediated genetic transformation of direct somatic embryos. A number of conditions related to the co-cultivation were tested with respect to maximizing transformation efficiency. The results showed that pH of the co-cultivation medium (5.7), the bacterial growth phase (optical density; $OD_{600}$ = 0.8), co-cultivation period (3 days), and acetosyringone concentration $(100\;{\mu}M)$ had positive effects on transformation. Selected plantlets were cultured on the medium at an elevated hygromycin level(30 mg/l). Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using hpt primers and by Southern hybridization using hpt-specific probe. The transgenic plantlets were obtained after 3-month cultivation and did not show any detectable variation in morphology or growth characteristics compared to wild-type plants.

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