• 폴리머
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• 제38권1호
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• pp.93-97
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• 2014

약물방출 고분자 코팅 스텐트는 수술후 재협착을 획기적으로 줄였지만, 약물방출이 균일한 구조체를 제작하는 것이 어렵고 체내에 구조체를 영구적으로 남겨야 하는 부담을 여전히 가지고 있다. 이를 해결하는 방안으로 생분해성 고분자로 스텐트를 제작하는 방법들이 활발하게 연구되고 있다. 본 연구에서는 조형가공기술(solid freeform fabrication, SFF)의 하나인 쾌속조형기법(rapid prototyping technique)의 3차원 플로팅(3D plotting) 기술을 이용하여 파크리탁셀(PTX) 약물을 함유한 폴리카프로락톤(PCL) 3차원 구조체를 제작하였고, 생분해성 PCL 고분자로부터 PTX의 방출거동과 스텐트 제작 가능성을 고찰하였다. 약물을 포함한 구조체의 표면특성을 SEM으로 확인한 결과 굴곡이 자연스럽고 매끄러운 표면을 가지고 있었다. FTIR을 통해서 약물이 성공적으로 구조체에 포함되었음을 확인하였고, NMR과 HPLC를 통해서 PCL 구조체 중의 PCL함량과 PTX의 서서히 방출됨을 확인되었다. 또한 세포실험을 통해 구조체에서 방출된 약물이 생물학적으로 활성을 유지하고 있으며, 반복제작된 구조체에서도 균일한 활성의 약물이 방출됨을 확인하였다. 이와같은 쾌속조형기법을 이용하여 약물을 포함하는 구조체를 제작하고 분석함으로써, 생분해성 고분자 스텐트로서의 적용가능성을 제시하였다.

• Molecules and Cells
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• 제21권1호
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• pp.104-111
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• 2006

Gene therapy with nonviral vectors using the suicide gene/prodrug activating system of herpes simplex virus type-1 thymidine kinase (HSV1-TK)/ganciclovir (GCV) is inefficient in killing malignant tumor cells due to two major factors: (a) an unsatisfactory bystander effect; (b) short-lived expression of the protein. To study the capacity of the protein transduction domain (PTD) of HIV-1 TAT protein to enhance HSV1-TK/GCV cancer gene therapy, we constructed three fusion proteins TAT-TK, TK-TAT and TK. TAT-TK retained as much enzyme activity as TK, whereas that of TK-TAT was much lower. TAT-TK can enter HepG2 cells and much of it is translocated to the nucleus. The transduced HepG2 cells are killed by exogenously added GCV and have bystander effects on untransduced HepG2 cells. Most importantly, the introduced recombinant protein is stable and remains functional for several days at least, probably because nuclear localization protects it from the cytoplasmic degradation machinery and provides access to the nuclear transcription machinery. Our results indicate that TAT fusion proteins traffic intercellularly and have enhanced stability and prodrug cell killing activity. We conclude that TAT has potential for enhancing enzyme prodrug treatment of liver cancers.

• Asian Pacific Journal of Cancer Prevention
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• 제15권23호
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• pp.10299-10306
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• 2015

The esophageal squamous cell carcinoma (ESCC) is thought to develop through a multi-stage process. Epigenetic gene silencing constitutes an alternative or complementary mechanism to mutational events in tumorigenesis. Posttranscriptional regulation of human leukocyte antigen class I (HLA-I) and antigen processing machinery (APM) proteins expression may be associated with novel epigenetic modifications in cancer development. In the present study, we determined the expression levels of HLA-I antigen and APM components by immunohistochemistry. Then by a bisulfite-sequencing PCR (BSP) approach, we identified target CpG islands methylated at the gene promoter region of APM family genes in a ESCC cell line (ECa109), and further quantitative analysis of CpG site specific methylation of these genes in cases of Kazakh primary ESCCs with corresponding non-cancerous esophageal tissues using the Sequenom MassARRAY platform. Here we showed that the development of ESCCs was accompanied by partial or total loss of protein expression of HLA-B, TAP2, LMP7, tapasin and ERp57. The results demonstrated that although no statistical significance was found of global target CpG fragment methylation level sof HLA-B, TAP2, tapasin and ERp57 genes between ESCC and corresponding non-cancerous esophageal tissues, there was significant differences in the methylation level of several single sites between the two groups. Of thesse only the global methylation level of LMP7 gene target fragments was statistically higher ($0.0517{\pm}0.0357$) in Kazakh esophageal cancer than in neighboring normal tissues ($0.0380{\pm}0.0214$, p<0.05). Our results suggest that multiple CpG sites, but not methylation of every site leads to down regulation or deletion of gene expression. Only some of them result in genetic transcription, and silencing of HLA-B, ERp57, and LMP7 expression through hypermethylation of the promoters or other mechanisms may contribute to mechanisms of tumor escape from immune surveillance in Kazakh esophageal carcinogenesis.

• 한국정보전자통신기술학회논문지
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• 제10권3호
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• pp.225-231
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• 2017

본 연구는 과수원에서 높은 곳에서의 작업을 진행함에 있어 안정적인 고소작업 기계차의 제작을 위하여 진행하였다. 과수원의 경우 비포장도로 및 경사진 지면이 많아 고소작업 기계차가 과수원에서 작업함에 있어 전복이 되거나 안전성에 관하여 문제가 되고 있는 부분을 해결하기 위함이다. 보다 안정적인 작업을 위하여 Auto leveling system을 설계 하였고 구조해석을 하는 도중 우리가 원하는 기준에 부합하지 않아 수평센서를 추가적으로 설치하였다. INVENTOR를 바탕으로 3D 모델링을 통해 구조해석을 한 결과 작업자가 작업할 수 있는 최대 기울어짐 정도는 좌측 116.49Mpa, 우측 118.26Mpa로 나타났다.

• 대한기계학회논문집A
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• 제38권12호
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• pp.1379-1385
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• 2014

제품의 수명시험 전에 소요될 시험시간을 대강이라도 예측할 수 있으면 비용을 측정하여 시험을 어떻게 진행할지 판단하는데 도움이 된다. 기계부품의 경우의 신뢰도예측은 시스템의 고장 혹은 열화 메커니즘이 복잡하고 보편적인 데이터베이스가 존재하지 않기 때문에 수행이 난해하다. 본 연구는 유압 액추에이터와 공압 액추에이터에 각각 설치되는 탄성 U 형 씰을 대상으로 수명예측을 NSWC에서 제안하는 고장물리를 고려한 고장률 모형과 현장 데이터베이스를 활용하여 수행하였다. 그 결과들을 검증하기 위해 예측된 수명과 시험된 수명데이터들을 비교하였다. 본 연구는 고장률 모형에 포함된 각종 계수 값들을 결정하는 과정과 씰의 수명에 영향을 주는 인자들의 개별 민감도를 분석하고 그 미비점을 고찰하였다.

• Tribology and Lubricants
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• 제31권2호
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• pp.35-41
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• 2015

A water-pump located in the cooling area of a car circulates cooling water. A particular bearing element, known as a water-pump bearing, installed in the rotating part carries the entire load. The failure of this water-pump bearing has a direct impact on the failure of the automobile engine, and so securing its reliability is crucial. Several researchers have examined the design principles of the water-pump bearing, but there are no reports on the life characteristic of the bearing yet. Herein, we report the construction of test equipment to reproduce the spalling of the roller contact, which is the main failure mode of the chosen water-pump bearing. We chose the radial load as an accelerated stress factor and validated the failure mode by monitoring the surface defects. We conducted the accelerated life test after determining the accelerated stress level through a combination of finite element analysis and a preliminary test. In the life tests, we used an accelerometer to perform failure diagnosis. In the last stage of this study, we present a statistical reliability analysis. Thus, we fully estimated the shape parameter of the water-pump bearing, accelerating level on the load , and the lifetime (MTTF and B₁₀ life) under real use conditions, and finally proposed an interval estimation value considering the uncertainty of the estimated value.

• 성인간호학회지
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• 제20권3호
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• pp.365-374
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• 2008

Purpose: This study was conducted to evaluate quality of sleep and to assess the factors that influence quality of sleep in surgical ICU. Methods: The subject of the study were consisted 109 adult patients who admitted to surgical ICU. The data were collected from May 20 to December 10, 2007 by structured questionnaires. The data were analyzed with descriptive analysis, paired t-test, Pearson correlation coefficient and stepwise multiple regression. Results: The score of quality of sleep was 4.57 point. The main sleep disturbance factors related to quality of sleep in surgical ICU inpatient were sleep time, machinery alarm and noise(adjusted $R^2$=33.2). Conclusion: Based on the finding of this study, it is needed to develop a nursing intervention program that including to promote quality of sleep and to decrease machinery alarm and noise in surgical ICU.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1995년도 추계학술대회
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• pp.47-51
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• 1995

• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 제39회 하계학술대회
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• pp.729-730
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• 2008

Recently, contactless power transfer system is widely used in many industry applications such as automated guided vehicles and medical applications. In this paper, we derived electrical parameters and analyzed output characteristics of contactless power transfer device with resonant converter for levitation / propulsion system. And, the characteristics analysis results are satisfied with output required specifications of analysis model.

• Journal of Biosystems Engineering
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• 제32권2호
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• pp.115-120
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• 2007

The adhesive performance of marine mussel extract was compared with that of the commercial medical adhesives for fastening biotissue (SIS) bandages. Joints bonded with mussel extract showed stronger strength than those bonded with other commercial adhesives. The bonding strength was much improved when the moisture in the Joint was reduced using superporous hydrogel regardless of curing time under humid condition ($37^{\circ}C$ and 91% relative humidity). Water inflow into the SIS joint seemed to degrade the performance of mussel extracts adhesive especially for the joints of short period of curing time. The strength of SIS joints coated with surgical sealant was well maintained after being exposed to water inflow.