• Journal of Embryo Transfer
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• v.19 no.3
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• pp.191-199
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• 2004

This study was conducted to examine in vitro development of porcine embryos constructed by the microinjection of cultured fetal fibroblast cells into porcine oocytes matured in vitro. Single fetal donor cells were deposited into the perivitelline space of enucleated oocytes, followed by electrical fusion and activation. Activated embryos were cultured in NCSU-23 medium supplemented with 5％ FBS, at 38.5$^{\circ}C$ for 6 to 8 days in 5％ $CO_2$ and air. In experiment 1, fusion rates of nuclear transfer embryos did not differ for fetal fibroblast cells incubated in 5％ FBS + NCSU-23 or 5％ FBS + TL Heaps medium, nor did fusion rates of donor cells differ between 1-8 hr incubation durations. Fusion rates for the four treatment subclasses ranged from 72.1％ to 78.0％. In experiment 2, Pre-synchronization in medium containing 0.1 $\mu\textrm{g}$/m Hoechst 33342 an increase from 0 and 8 versus 15 h culture an increased percentage of porcine fibroblast cells in G2/M at the end of the synchronization period (12.4％, 17.5％ and 47.6％). Neither an increase in the concentration of H 33342 (0.2-1.6 $\mu\textrm{g}$/$m\ell$) nor a longer exposure time (12h, 18h and 24h) increased the proportion of porcine G2/M fibroblasts. In experiment 3, fusion rates did not differ significantly far nuclear transfer embryos constructed using donor cells cultured in 5％ FBS + NCSU-23 medium for 1-2, 6-8 or 12-14 days (60.0％, 73.3％ and 62.5％), respectively. The cleavage rate for nuclear transplant embryos using fetal fibroblast cells cultured for 1-2 days was 44.0％, significantly less than 56.7％ and 50.0％. for 6-8 or 12-14 days duration of culture, respectively. In experiment 4, the proportions of nuclear transfer embryos that developed to the $\geq$2 cell and to the blastocyst stage were not affected significantly by culture medium (5％ FBS + NCSU-23 or 5％ FBS + TL-Heaps) or by $O_2$ concentration of the culture (5％ vs 10％). Rates of development to the $\geq$2 cell stage ranged from 65.9％ to 70.1％, and development rates to the blastocyst stage ranged from 9.8％ to 12.5％ for the four treatment subclasses. Developmental rate was highest for embryos cultured in 5％ FBS + NCSU-23 under a gas atmosphere of 5％ $O_2$ in air.

• Journal of the Korean Society of Costume
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• v.62 no.5
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• pp.125-137
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• 2012

This study examines the stage costumes of the creative musical 'Hyecho', which was performed six times in the Chung Ang Art Center hosted by Chung-Ang University's performance & media agency from December 20 to 22 in 2006. The stage costumes were made based on these basic design elements of stage costumes that were dyed : line, silhouette, material, and color. First, the creative musical 'Hyecho' presents a new form of fusion theatre, which blends Korean traditional music and dance with videos. Thereby, the musical has opened a new phase in Korea's creative performing arts. Second, in musical performances that combines dance and songs, stage costumes should be made not only to attract the audience's eyes, but also not to interrupt actors' vocalizations and movements by taking their physical features into account. Also, costumes should be made in consideration of their relationships with visual elements, including stage settings and lighting. Third, the musical features fusion-style stage costumes, which combines Hanbok, the Korean traditional costume, and Indian traditional costumes with modern costumes. For the line and silhouette, costumes show the beauty of curves through the curves of Hanbok and India's traditional costumes and also through irregular pleats of pants. Also, by using cotton materials, which is easy to dye and not readily deformed, costumes feature colors that are found in nature through a gradation dyeing technique. In doing so, it offers visual amusement to the audience by making stage costumes look like a beautiful painting. Fourth, the stage costumes of the chorus feature costumes that use lining and pleated skirts using belts, and various accessories, including necklaces that use strings in order to express evil spirits. Since there is not much time to change costumes during a performance, using such items are helpful to show the unique characteristics of actors effectively during the limited time. Also, coordinating with the lighting director allows the costume designer to make better costumes for the chorus and make the performance more dramatic. Finally, it was not necessary to wash the costumes of the chorus of the fusion musical Hyecho 2006 since it was performed only six times. However, when using dyed costumes for the long-term performance, it might cause problems such as bleaching that result from the washing of costumes and low durability that can result in the deformation of costumes. As performing arts are made in various forms and are diversified, it is needed for stage costumes to change accordingly through new attempts and various ways of expression.

• Journal of the Korea Computer Graphics Society
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• v.14 no.4
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• pp.35-39
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• 2008

A computer supported collaborative system provides with a shared virtual workspace over the Internet where its remote users cooperate in order to achieve their goals by overcoming problems caused by distance and time. VRMMS (Virtual Reality Molecular Modeling System) [1] is a VR based collaborative system where biologists can remotely participate in and exercise molecular modeling tasks such as viewing three dimensional structures of molecular models, confirming results of molecular simulations and providing with feedbacks for the next simulations. Biologists can utilize VRMMS in executing molecular simulations. However, first-time users and beginners need to spend some time for studying and practicing in order to skillfully manipulate molecular models and the system. The best way to resolve the problem is to have a face-to-face session of teaching and learning VRMMS. However, it is not practically recommended in the sense that the users are remotely located. It follows that the learning time could last longer than desired. In this paper, we propose to use Second Life [2] combining with VRMMS for removing the problem. It can be used in building a shared workplace over the Internet where molecular simulations using VRMMS can be exercised, taught, learned and practiced. Through the web, users can collaborate with each other using VRMMS. Their avatars and tools of molecular simulations can be remotely utilized in order to provide with senses of 'being there' to the remote users. The users can discuss, teach and learn over the Internet. The shared workspaces for discussion and education are designed and implemented in Second Life. Since the activities in Second Life and VRMMS are designed to realistic, the system is expected to help users in improving their learning and experimental performances.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.20 no.10
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• pp.1031-1041
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• 2009

A small microstrip monopole antenna for macro-micro frequency tuning over multiple bands is presented. The meander-shape antenna is fabricated on a conventional printed circuit board(FR-4, $\varepsilon_r=4.4$ and tan $\delta=0.02$). The antenna operates over WiBro(2.3~2.4 GHz) and WLAN a/b(2.4~2.5 GHz/5.15~5.35 GHz) service bands with an essentially constant antenna gain within each service band. Two diodes, a PIN diode and a varactor, are embedded into the antenna for frequency reconfiguration. The PIN diode is used for frequency switching(macro-tuning) between 2 GHz and 5 GHz bands while the varactor is used for frequency tuning(micro-tuning) within the service bands, 2.3~2.5 GHz and 5.15~5.35 GHz. Unwanted resonances between the two frequency bands(2 GHz and 5 GHz) are suppressed by filling up the gaps between the meander lines. The antenna gain is essentially constant and higher than 2 dBi within each service band. The measured performance of the proposed antenna system suggests the macro-micro frequency tuning techniques be useful in reconfigurable wireless communication systems.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.3
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• pp.395-402
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• 2001

A fusant FG-21 was selected on the basis of higher ${\gamma}-GTP$ activity following fusion process between SM-2 and SM-10 of Bacillus subtilis mutants. ${\gamma}-GTP$ activity of the mutant FG-21 was increased up to 612 U/mL when grown for 36 hr at $37^{\circ}C$ in culture media containing 1% glycerol 1% glycerol, 1% peptone, 0.1% citric acid, 5 mM $K_2HPO_4$, 1 mM $FeCl_3$, 1 mM $MgCl_2$, 1 mM $NH_4Cl$, pH 7.0. In fusnat FG-21, the ratio of protein to total sugar contents for biopolymer A was 38 to 59. for biopolymer B from parental strains it was 19 to 78. Fructose contents determined by HPLC were $573.7\;\mu\textrm{g}/mg\;and\;764.4\;\mu\textrm{g}/mg$ for biopolymer A and B, respectively. And glutamic acid content were $163.7\;\mu\textrm{g}/mg\;and\;94.6\;\mu\textrm{g}/mg$ for biopolymer A and B, respectively. In fusant FG-21, the ratio of fructose to glutamic acid contents for biopolymer A was 78 to 22. For biopolymer B from parental strains it was 89 to 11.

• Journal of Broadcast Engineering
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• v.16 no.1
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• pp.14-22
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• 2011

The conventional satellite image fusion methods usually add the same amount of higher frequency components extracted from the panchromatic image to all the multispectral images. However, it is noted that each of multispectral images has different amount of overlap with the panchromatic image in terms of its spectrum, and also has different intensities. Thus giving the same amount of high frequency contents to all the spectral bands does not match with this observation, which causes color distortion in the fused image. In this paper, we propose a new wavelet-domain satellite image fusion algorithm that can compensate for these differences in intensity and spectrum overlap. For the compensation of intensity differences, we first estimate the high resolution multispectral images from P, considering the relative intensity ratios. For the compensation of the amount of spectral overlap, their wavelet coefficients are appended to the conventional wavelet-domain method where the coefficients for the addition is determined by the amount of spectrum overlap. Experiments are conducted for the IKONOS satellite images whose spectrums are well known, and the results show that the proposed algorithm gives higher PSNR and correlation coefficients compared to the conventional methods.

• Korean Journal of Optics and Photonics
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• v.18 no.4
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• pp.246-255
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• 2007

The floating image system (FIS) is a device to display input source in the space between fast surface of the display and an observer and it provides pseudo 3D depth to an observer when input source as real object or 2D image was displayed through the optical lens system in the FIS. The Advanced floating image system (AFIS) was designed to give more effective 3D depth than existing FIS by adding front and rear depth cues to the displayed stereogram, which it was used as input source. The magnitude of disparity and size of stereogram were strongly related each other and they have been optimized for presenting 3D depths in a non-optical lens systems. Thus, if they were used in optical lens system, they will have reduced or magnified parameters, leading to problem such as providing incorrect 3D depth cues to an observer. Although the size of stereogram and disparity were demagnified by total magnifying power of optical system, the viewing distance (VD) from the display to an observer and base distance (BD) for the gap between the eyes were fixed. For this reason, the quantity of disparity in displayed stereogram through the existing FIS has not kept the magnifying power to the total optical system. Therefore, we proposed the methods to provide correct 3D depth to an observer by compensating quantity of disparity in stereogram which was satisfied to keep total magnifying power of optical lenses system by AFIS. Consequently, the AFIS provides a good floating depth (pseudo 3D) with correct front and rear 3D depth cues to an observer.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.45 no.4
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• pp.50-58
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• 2008

In this paper, we propose a color transient improvement (CTI) algorithm based on image fusion to improve the color transient in the television(TV) receiver or in the MPEG decoder. Video image signals are composed of one luminance and two chrominance components, and the chrominance signals have been more band-limited than the luminance signals since the human eyes usually cannot perceive changes in chrominance over small areas. However, nowadays, as the advanced media like high-definition TV(HDTV) is developed, the blurring of color is perceived visually and affects the image quality. The proposed CTI method improves the transient of chrominance signals by exploiting the high-frequency information of the luminance signal. The high-frequency component extracted from the luminance signal is modified by spatially adaptive weights and added to the input chrominance signals. The spatially adaptive weight is estimated to minimize the ${\iota}_2-norm$ of the error between the original and the estimated chrominance signals in a local window. Experimental results with various test images show that the proposed algorithm produces steep and natural color edge transition and the proposed method outperforms conventional algorithms in terms of both visual and numerical criteria.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.11 no.5
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• pp.608-614
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• 2018

In addition to the diffusion of ICT technology, various protocols of short range wireless communication technology are being applied for efficient information operation. However, due to limitations of short-range wireless communication, communication is not smooth in places where frequency environment is poor, such as frequency confusion and warehouse type factory. When an alternative is needed. The development of LED technology and expansion of infrastructure through LED based visible light communication is attracting attention as an alternative and spreading the usage in wide range now a days. In addition, the infrastructure has been expanded with solar panels in response to the development of smarthome built-in with renewable energy. In this situation, visible light communication using PD has been limitedly applied in a near environment where the receiving angle of the PD and the ambient light ensure the LoS and the influence of the ambient light is small. In order to solve this problem, we have implemented visible light communication using LED lighting with large current infrastructure and solar panel with large receiving area, and proposed a circuit for restoring accurate data even in ambient light. Through this study results, it is expected that visible light communication can be more widely used and this result used as the base data for visible light communication research using the solar panel as the receiver.

• Cartoon and Animation Studies
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• s.46
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• pp.207-231
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• 2017

Printmaking is a field of fine arts and is placed on a vague boundary that is perceived as a commercial product with a popular character due to the speciality of editions. Advances of modern science and technology has developed a new technique of printmaking, and the fusion of printmaking and computer has shown the possibility of reproduction art. Reproduction printmaking has been heavily influenced by photography and extended areas, and the various forms of printing have brought about many changes and attempts by stimulating the possibilities of indirect art at various angles. As the history of printmaking and technology closely relate, the development of computer makes widespread expansion of plural artistry, technological and artistic change. A new conceptual shape can be created on the copied image simply by placing the material of the print on the smoothly flat surface expressed in digital form. The process and the result of such work show the area of unique work which is different from the value of the $\grave{a}$ la carte art or the characteristics of the material given by the print. The deprecated perception of reproducibility evaluated the value of the work in a direct sense of printmaking. It is undeniable that it is devalued by a bundle of works regardless of the value of each edition. However, the physical properties of the prints on the paper are brought up with hand drawings drawn on the canvas by hand. And it becomes an opportunity to show new aspect and change through the process of combining digital print information on paper. The diversity of media is sometimes a controversy of identity between art and technology. In the future, it should be discussed how the limit of the media which can be enjoyed in the field of art can be set as a standard.