• Korean journal of communication and information
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• v.52
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• pp.48-68
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• 2010

In covering the controversies surrounding the so-called cyber defamation laws, the Korean press offered competitive frames in terms of values (security vs. freedom of speech) and attributional patterns (episodic vs. thematic attribution). By attending to core values and attributional patterns as two essential components of news frames, this study explored the cognitive and affective processes of value and attributional framing and their effects on issue opinion. According to a 3-group online experiment, first, it was found that core values increased the perceived importance of relevant beliefs, which further affected individuals' attitudes toward the laws. The affective effects of core values were also found marginally significant. The value of security increased the intensity of anger toward deviant netizens (so-called defamatory repliers), and it further increased individuals' support for the laws. It was not substantiated, however, that individualistic attribution, than social attribution, would provoke stronger anger toward defamatory repliers. Instead, episodic frames appeared to be more effective in driving issue opinion as indicated by the value frame.

• Korean journal of communication and information
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• v.52
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• pp.5-26
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• 2010

This study explores how major Korean television evening news report, interpret and evaluate the Korean Grand Canal Project(KGCP). For this research, 488 news clips regarding the KGCP are selected among the MBC, KBS and SBS daily evening news. As a result, the findings are as follows: First, the ratios of analyzing reports for MBC, brief reports for KBS, and straight news for SBS are outstanding. At the same time, the three news companies show that they are against the KGCP through the reporting attitudes. However, the most frequently broadcasted news frames is the authoritative political execution for both MBC and KBS, and the political agenda for SBS news respectively. In terms of the most frequently interviewed group for all three news is the government and ruling Grand National Party; they are the most favor of the authoritative political executions and the political agenda news frames. However, the NGO groups and the opposition parties which are against the KGCP support "Process" in terms of the frames activity. Finally, MBC speaks out the frame activity of "Process", supporting the political opposition parties and NGO groups. On the country, both KBS and SBS speak up the government and ruling party, supporting the KGCP, stressing frame activity of "Aspiration". This it concludes that both KBS and SBS are short of the social responsibilities as a social media mediator for the social disputes between the government and the NGO groups, including opposition parties.

• The Korean Journal of Mycology
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• v.15 no.1
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• pp.29-37
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• 1987

Cellulolytic mocrooganisms were isolated from the cellulose-cultural properties. Among them, Aspergillus clavatus with the highest cellulase activity was identified by the morphological characteristics and it's enzyme activities were compared on the various cultural conditions. It was found that the induction of carboxymethylcellulase(CMCase), avicelase and salicinase in CMC liquid media showed the highest enzyme acitivity on five day's cultivation at $30^{\circ}C$ and thereafter their activities were gradually decreased with time. After crude extracellular enzymes precipitated with the 70% saturated ammonium sulfate solution were dialyzed with 20 mM acetate buffer pH 6.0, each crude enzyme was examined. The optimal activities of CMCase and avicelase were both found to be at $50^{\circ}C$ and pH 6.0. Their thermal stability was appeared at the $50^{\circ}C$. CMCase and avicelase had the maxima activities with 1.5% and 2.2% substrate concentration, respectively. The concentration of 5 mM $Mg^{++}$ or $Ca^{++}$ was found to have a maximum cellulase activity and its activity was inhibited with more than 5 mM $Cu^{++}$ and $Zn^{++}$ concentration. Cellulase activity was also inhibited sensitively by the inhibitors such as 2-mercaptoethanol, iodine and sodium azide.

• Korean Journal of Microbiology
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• v.31 no.2
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• pp.105-112
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• 1993

Plesiomonas shigelloides distributed in the aquatic systems was isolated and identified in this study and compared with the c1inica] isolates in view of their physiological characteristics, Biochemica] charactristics of the isolates of P. shigelloides one sample taken from Gupo and two samples taken from Mu]gum, were studied. However, none was isolated in Haeundae, Dadaepo, Kangdong and Nakdong estuary. The isolated bacteria had an optimum growth condition in peptone water of $25~35^{\circ}C$, pH 7.5-8.0 and 1% NaCI concentration. The cell grew most properly on the selective enrichment media which were made from adding inositol to peptone water. DNase was s]owly produced and the results were different from those of other studies. The components of the fatty acid were 3% of 3-hydroxy]ated fatty acid containing $C_{12}~C_{18}$. 0-10% cyclopropane ($C_{17:0}$), 25~30% hexadecanoic acid ($C_{16:0}$), 32~43% hexadecenoic acid ($C_{16:1}$), 1~2% octadecanoic acid ($C_{16:0}$), and 9~14% octadecenoic acid ($C_{18:1}$). Bacterio]ogica] characteristics, susceptibility of antibiotics, and the components of fatty acid of the c1inica] isolates were similar to those of the strains isolated from the aquatic systems. The strains isolated from c1inica] sources degraded lactose more fast than those isolated from the aquatic systems. There existed resistant bacteria to chlorampenicol in the strains from patients, but there were no resistant bacteria in the strains from the aquatic systems. The components of fatty acid of the clinical isolates were 0~2% $C_{17:0 cyclo}$ and 2~3% $C_{18:0}$, but those of the strains from the aquatic systems were 2~10% and 1~2%, respectvely, which showed the quantitative difference between both components.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.3
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• pp.668-676
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• 2016

This study reviewed the planning characteristics of contemporary Japanese middle school architecture on which related studies are insufficient, aiming to obtain new ideas for planning Korean middle school facilities. Fourteen case schools built after 1990s were selected and analyzed. They were divided into learning-living space and other major spaces. The planning characteristics of the case schools are summarized as follows 1) The case schools were classified into two categories, departmentalized classroom type (D type) and usual with variation type (UV type) by school system. These categories can also be the classification standard for basic architectural characteristics in learning and living space of case schools. 2) D type case schools have departmentalized classrooms, home base, media space and teacher's space for learning-living space. D type case schools are divided into 'attached-to-classroom type' and 'separate type' depending on the adjacency of the home base and departmentalized classroom. 3) UV type case schools have multipurpose space around the classroom for learning-living space and can be divided into two types, i.e., 'directly adjacent' and 'separate', depending on the connectivity to classroom of multipurpose room. 4) Specialized classrooms are designed to have the openness to the public and the own characteristics of school subjects strengthened and show the spatial differentiation with connected ancillary spaces. 5) Libraries are designed as complex zones grouped with computer labs, audio visual rooms and multipurpose halls not as a single room and as open plan not with a closed wall. 6) The gymnasium is the basic sports facility with a martial arts room and outdoor pool, which are for after-school activities as well as physical education class. 7) The terrace, balcony and outdoor stairs are frequently used architectural vocabularies as diverse outdoor spaces with a variety of functions.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.6
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• pp.802-808
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• 2013

Here we tried to uncover the potential anti-lipogenic effect and the underlying mechanism of Phaseolus angularis seed in a cellular model of nonalcoholic fatty liver disease (NAFLD) induced in HepG2 cells. Ethanol extract of Phaseolus angularis seed (JSD) was prepared. HepG2 cells were incubated in palmitate containing media to induce intracellular lipid accumulation, and co-treated with JSD for 16 hrs before examine intracellular lipid content. In control group, the cells were not co-treated with JSD. We measured the effects of JSD on liver X receptor ${\alpha}$ ($LXR{\alpha}$) and sterol regulatory element-binding transcription factor-1c (SREBP-1c) expression, transcription level of lipogenic genes, including acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-CoA desaturase-1 (SCD-1), and AMP-activated protein kinase (AMPK) activation in HepG2 cells. JSD markedly reduced palmitate-induced intracellular lipid accumulation in HepG2 cells. JSD suppressed $LXR{\alpha}$/SREBP-1c expression, and SREBP-1c mediated induction of ACC, FAS, and SCD-1. Furthermore, JSD activated AMPK, which plays a major role in the control of hepatic lipid metabolism. Taken together, it is suggested that JSD has a potential to alleviate hepatic steatosis, at least in part, by suppressing $LXR{\alpha}$/SREBP-1c mediated induction of lipogenic genes. In addtion, the anti-lipogenic potential may be associated with activation of AMPK. Therefore, the Phaseolus angularis seed could be applied as a potential therapeutics for NAFLD with additional clinical studies.

• Journal of Broadcast Engineering
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• v.14 no.4
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• pp.450-462
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• 2009

So far, many methods for segmenting single images or video have been proposed, but few methods have dealt with multiple images with analogous content. These images, which we term consistent scene images, include concurrent images of a scene and gathered images of a similar foreground, and may be collectively utilized to describe a scene or as input images for multi-view stereo. In this paper, we present a method to segment these images with minimum user input, specifically, manual segmentation of one image, by iteratively propagating information via multi-level cues with adaptive confidence depending on the nature of the images. Propagated cues are used as the bases to compute multi-level potentials in an MRF framework, and segmentation is done by energy minimization. Both cues and potentials are classified as low-, mid-, and high- levels based on whether they pertain to pixels, patches, and shapes. A major aspect of our approach is utilizing mid-level cues to compute low- and mid- level potentials, and high-level cues to compute low-, mid-, and high- level potentials, thereby making use of inherent information. Through this process, the proposed method attempts to maximize the amount of both extracted and utilized information in order to maximize the consistency of the segmentation. We demonstrate the effectiveness of the proposed method on several sets of consistent scene images and provide a comparison with results based only on mid-level cues [1].

• Economic and Environmental Geology
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• v.53 no.2
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• pp.213-220
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• 2020

When CO₂ foam is injected into the saline aquifer, the relative permeability of CO₂ decreases and its viscosity increases, thereby reducing mobility in porous media and ultimately improving CO₂ storge with enhanced sweep efficiency. In general, surfactants were used to fabricate CO₂ foam. Recently, nanoparticles have been used to form stable foam than surfactant. This paper introduces CO₂ storage technology using nanoparticle stabilized CO₂ foam. If the surface of the hydrophilic nanoparticles is partially modified into a CO₂-philic portion, the particles have an affinity for CO₂ and water, thus forming a stable CO₂ foam even in deep saline aquifers under high temperature and high salinity conditions, thereby it can be stored in the pores of the rock. In terms of economics, injection method using nanopaticle-stabilized CO₂ foam is more expensive than the conventional CO₂ injection, but it is estimated that it will have price competitiveness because the injection efficiency is improved. From an environmental point of view, it is possible to inject chemical substances such as surfactants and nanomaterials into aquifers or reservoirs for specific purposes such as pollutant removal and oil production. However, some studies have shown that nanoparticles and surfactants are toxic to aquatic animals, so environmentally proven substances should be used. Therefore, further research and development will be needed to study the production and injection of nanoparticle-stabilized CO₂ foam that are environmentally safe and economically reasonable.

• Microbiology and Biotechnology Letters
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• v.43 no.2
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• pp.97-104
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• 2015

Vinegar is a widely used acidic seasoning and can be manufactured using various methods and bases, including cereals, wheat, and fruits. Most studies on vinegar have been conducted to evaluate its antioxidant activity. In the present study, fermented dark vinegar (FDV) produced from unpolished rice was examined for its antimicrobial activity, biochemical content, including the amounts of sugar, total soluble sugar, organic acid, and free amino acids, and pH and physiological activity. The antimicrobial efficiency of FDV was assessed using the paper disc-agar diffusion method. FDV exhibited strong antimicrobial activity against the pathogenic bacteria and yeast strains that were tested. In fact, the activity of FDV was shown to be higher than that of the commercial antibiotics carbenicillin (50 µg/ml) and tetracycline (50 µg/ml) against Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Yersinia enterocolitica, and Lodderomyces elongisporus. The antioxidant activity of FDV and ascorbic acid was evaluated. Using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, we found that FDV has the highest activity of the antioxidants. After spreading FDV onto tryptic soy broth and yeast extract-peptone-dextrose agar media, the microbial strains were isolated and characterized through physiological and biochemical analysis. Based on 16S ribosomal DNA sequence analysis, the isolated microorganisms exhibited a close similarity to Acetobacter papayae, Acetobacter pasteurianus, and Acetobacter peroxidans.

• Journal of Microbiology and Biotechnology
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• v.19 no.5
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• pp.502-510
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• 2009

Although the Escherichia coli heat-labile enterotoxin B subunit (LTB) has already been expressed in several different systems, including prokaryotic and eukaryotic organisms, studies regarding the synthesis of LTB into oligomeric structures of pentameric size in the budding yeast Saccharomyces cerevisiae have been limited. Therefore, this study used a functional signal peptide of the amylase 1A protein from rice to direct the yeast-expressed LTB towards the endoplasmci reticulum to oligomerize with the expected pentameric size. The expression and assembly of the recombinant LTB were confirmed in both the cell-free extract and culture media of the recombinant strain using a Western blot analysis. The binding of the LTB pentamers to intestinal epithelial cell membrane glycolipid receptors was further verified using a GM1-ganglioside enzyme-linked inmmunosorbent assay (GM1-ELISA). On the basis of the GM1-ELISA results, pentameric LTB proteins comprised approximately 0.5-2.0% of the total soluble proteins, and the maximum quantity of secreted LTB was estimated to be 3 mg/l after a 3-day cultivation period. Consequently, the synthesis of LTB monomers and their assembly into biologically active aligomers in a recombinant S. cerevisiae strain demonstrated the feasibility of using a GRAS microorganism-based adjuvant, as well as the development of carriers against mucosal disease.