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http://dx.doi.org/10.4014/mbl.1504.04005

Antimicrobial Efficacy of Fermented Dark Vinegar from Unpolished Rice  

Choi, Hakjoon (Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, Chosun University)
Gwak, Gyeongja (Department of Complementary and Alternative Medicine, Chosun University)
Choi, Dabin (Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, Chosun University)
Park, Jaeyoung (Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, Chosun University)
Cheong, Hyeonsook (Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, Chosun University)
Publication Information
Microbiology and Biotechnology Letters / v.43, no.2, 2015 , pp. 97-104 More about this Journal
Abstract
Vinegar is a widely used acidic seasoning and can be manufactured using various methods and bases, including cereals, wheat, and fruits. Most studies on vinegar have been conducted to evaluate its antioxidant activity. In the present study, fermented dark vinegar (FDV) produced from unpolished rice was examined for its antimicrobial activity, biochemical content, including the amounts of sugar, total soluble sugar, organic acid, and free amino acids, and pH and physiological activity. The antimicrobial efficiency of FDV was assessed using the paper disc-agar diffusion method. FDV exhibited strong antimicrobial activity against the pathogenic bacteria and yeast strains that were tested. In fact, the activity of FDV was shown to be higher than that of the commercial antibiotics carbenicillin (50 µg/ml) and tetracycline (50 µg/ml) against Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Yersinia enterocolitica, and Lodderomyces elongisporus. The antioxidant activity of FDV and ascorbic acid was evaluated. Using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, we found that FDV has the highest activity of the antioxidants. After spreading FDV onto tryptic soy broth and yeast extract-peptone-dextrose agar media, the microbial strains were isolated and characterized through physiological and biochemical analysis. Based on 16S ribosomal DNA sequence analysis, the isolated microorganisms exhibited a close similarity to Acetobacter papayae, Acetobacter pasteurianus, and Acetobacter peroxidans.
Keywords
Fermented dark vinegar; antimicrobial activity; antioxidant activity; human pathogenic microorganisms; pathogenic bacteria; 16S rDNA;
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1 Fukuyama N, Jujo S, Ito I, Shizuma T, Myojin K, Ishiwata K, et al. 2007. Kurozu moromimatsu inhibits tumor growth of Lovo cells in a mouse model in vivo. Nutrition 23: 81-86.   DOI
2 Banat IM, Samarah N, Murad M, Horne R, Banerjee S. 1991. Biosurfactant production and use in oil tank clean-up. World J. Microbiol. Biotechnol. 7: 80-88.   DOI
3 Kim S-H, Cho H-K, Shin H-S. 2012. Physicochemical properties and antioxidant activities of commercial vinegar drinks in Korea. Food Sci. Biotechnol. 21: 1729-1734.   DOI
4 Amalfi M, Yombiyeni P, Decock C. 2010. Fomitiporia in sub-Saharan Africa: morphology and multigene phylogenetic analysis support three new species from the Guineo-Congolian rainforest. Mycologia 102: 1303-1317.   DOI
5 Seki T, Morimura S, Tabata S, Tang Y, Shigematsu T, Kida K. 2008. Antioxidant activity of vinegar produced from distilled residues of the Japanese liquor shochu. J. Agric. Food Chem. 56: 3785-3790.   DOI
6 Caceres PJ, Martinez-Villaluenga C, Amigo L, Frias J. 2014. Maximising the phytochemical content and antioxidant activity of Ecuadorian brown rice sprouts through optimal germination conditions. Food Chem. 152: 407-414.   DOI
7 Castillo S, Rosales M, Pohlenz C, Gatlin DM. 2014. Effects of organic acids on growth performance and digestive enzyme activities of juvenile red drum Sciaenops ocellatus. Aquaculture 433: 6-12.   DOI
8 Diana M, Rafecas M, Quílez J. 2014. Free amino acids, acrylamide and biogenic amines in gamma-aminobutyric acid enriched sourdough and commercial breads. J. Cereal Sci. 60: 639-644.   DOI
9 Goffman FD, Bergman CJ. 2004. Rice kernel phenolic content and its relationship with antiradical efficiency. J. Sci. Food Agr. 84: 1235-1240.   DOI
10 Hashimoto M, Obara K, Ozono M, Furuyashiki M, Ikeda T, Suda Y, et al. 2013. Separation and characterization of the immunostimulatory components in unpolished rice black vinegar (kurozu). J. Biosci. Bioeng. 116: 688-696.   DOI   ScienceOn
11 Kim J-H, Kim S-G, Kim S-S, Kim J-H, Park S-H, Nam K-H, et al. 2013. Analysis of the antibiotic resistance gene in Salmonella Typhimurium isolates from diseased pigs in Gyeongbuk province. Korean J. Vet. Serv. 36: 73-78.   DOI
12 Koleva II, van Beek TA, Linssen JP, de Groot A, Evstatieva LN. 2002. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis : PCA 13: 8-17.   DOI
13 Lee SB, Cha KH, Kim SN, Altantsetseg S, Shatar S, Sarangerel O, et al. 2007. The antimicrobial activity of essential oil from Dracocephalum foetidum against pathogenic microorganisms. J. Microbiol. 45: 53-57.
14 Kim SP, Kang MY, Nam SH, Friedman M. 2012. Dietary rice bran component gamma-oryzanol inhibits tumor growth in tumor-bearing mice. Molecul. Nutr. Food Res. 56: 935-944.   DOI   ScienceOn
15 Kitagaki H, Tsugawa M. 1999. 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging ability of sake during storage. J. Biosci. Bioeng. 87: 328-332.   DOI
16 Mateo E, Torija MJ, Mas A, Bartowsky EJ. 2014. Acetic acid bacteria isolated from grapes of South Australian vineyards. Int. J. Food Microbiol. 178: 98-106.   DOI
17 Mehni AM, Ketabchi S, Bonjar, GHS. 2014. Antibacterial activity and polyphenolic content of Citrullus colocynthesis. Int. J. Biosci. 190-196.
18 Nishidai S, Nakamura Y, Torikai K, Yamamoto M, Ishihara N, Mori H, et al. 2000. Kurosu, a traditional vinegar produced from unpolished rice, suppresses lipid peroxidation in vitro and in mouse skin. Biosci. Biotechnol. Biochem. 64: 1909-1914.   DOI
19 Tong LT, Katakura Y, Kawamura S, Baba S, Tanaka Y, Udono M, et al. 2010. Effects of Kurozu concentrated liquid on adipocyte size in rats. Lipids in Health and Disease 9: 134.   DOI
20 Shimoji Y, Kohno H, Nanda K, Nishikawa Y, Ohigashi H, Uenakai K, Tanaka T, et al. 2004. Extract of Kurosu, a vinegar from unpolished rice, inhibits azoxymethane-induced colon carcinogenesis in male F344 rats. Nutr. Cancer. 49: 170-173.   DOI
21 Vilar MJ, Virtanen S, Laukkanen-Ninios R, Korkeala H. 2015. Bayesian modelling to identify the risk factors for Yersinia enterocolitica contamination of pork carcasses and pluck sets in slaughterhouses. Int. J. Food Microbiol. 197: 53-57.   DOI
22 Shizuma T, Nagano M, Fujii A, Mori H, Fukuyama N. 2011. Therapeutic effects of four molecular-weight fractions of Kurozu against dextran sulfate sodium-induced experimental colitis. Turk J. Gastroenterol. 22: 376-381.   DOI
23 Shizuma T, Ishiwata K, Nagano M, Mori H, Fukuyama N. 2011. Protective effects of Kurozu and Kurozu Moromimatsu on dextran sulfate sodium-induced experimental colitis. Digest. Dis. Sci. 56: 1387-1392.   DOI
24 Verzelloni E, Tagliazucchi D, Conte A. 2007. Relationship between the antioxidant properties and the phenolic and flavonoid content in traditional balsamic vinegar. Food Chem. 105: 564-571.   DOI
25 Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 1991. 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 173: 697-703.   DOI
26 Woo K-S, Ko J-Y, Song S-B, Lee J-S, Oh B-G, Kang J-R, et al. 2010. Physicochemical characteristics of Korean traditional wines prepared by addition of Sorghum (Sorghum bicolor L. Moench) using different nuruks. J. Korean Soc. Food Sci. Nutr. 39: 548-553.   DOI
27 Zhang G, Malik VS, Pan A, Kumar S, Holmes MD, Spiegelman D, et al. 2010. Substituting brown rice for white rice to lower diabetes risk: a focus-group study in Chinese adults. J. Am. Diet. Assoc. 110: 1216-1221.   DOI
28 Shimoji Y, Tamura Y, Nakamura Y, Nanda K, Nishidai S, Nishikawa Y, et al. 2002. Isolation and identification of DPPH radical scavenging compounds in Kurosu (Japanese unpolished rice vinegar). J. Agric. Food Chem. 50: 6501-6503.   DOI
29 Monks JLF, Vanier NL, Casaril J, Berto RM, de Oliveira M, Gomes CB, et al. 2013. Effects of milling on proximate composition, folic acid, fatty acids and technological properties of rice. J. Food Compos. Anal. 30: 73-79.   DOI