• Title/Summary/Keyword: Mechanical Restriction

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Study on PDMS/Class Microthermostat Fabrication and Evaluation for Restriction Enzyme Reaction (제한효소 반응용 PDMS/유리 마이크로 항온조 제작 및 특성평가)

  • Jin Seok-Ho;Cho Yong-Jin;Ahn Yoomin
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.28 no.10
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    • pp.1598-1602
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    • 2004
  • In this paper, we report a microthermostat using PDMS (poly-dimethylsiloxane) and glass. This PDMS/glass chip is able to maintain constant temperature that is necessary for restriction enzyme reaction. Since PDMS is the low-cost and the mass-producible material and has very good biochemical compatibility, PDMS chip has more benefit than general Si chip. Heater was made of Au wiring patterned on Pyrex glass. A reaction chamber has a capacity of about 3 ${mu}ell$. We performed a restriction enzyme reaction by using the fabricated microthermostat and conventional method. Then, with the electrophoresis, we made a comparison between the result from the micro reactor and the one from conventional method.

PDMS/Glass Based DNA Microbiochip for Restriction Enzyme Reaction and Electrophoresis Detection (DNA의 제한효소 반응 및 전기영동 검출용 PDMS/유리 마이크로바이오칩)

  • Choi Joon-Young;Ahn Yoo Min;Hwang Seung-Yong
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.30 no.1 s.244
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    • pp.26-31
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    • 2006
  • This paper reports low-cost PDMS/glass based DNA microbiochip for the restriction enzyme reaction and its products detection using the capillary electrophoresis. The microbiochip ($25mm{\times}75mm$) has the heater integrated reactor ($5{\mu}{\ell}$) for DNA restriction enzyme reaction at $37^{\circ}C$ and the microchannel ($80\;{\mu}m{\times}100\;{\mu}m{\times}58mm$) for the capillary electrophoresis detection. It is experimentally confirmed that the digestion of the plasmid ($pGEM^{(R)}-4Z$) by the enzyme (Hind III and Sca I) is performed for less than 10 min and its electrophoresis detection is able to sequentially on the fabricated microbiochip.

A Study on Gear Forming Process for High Precision Automotive Seat Recliner (자동차용 고정밀 시트 리클라이너의 기어성형 공정에 관한 연구)

  • Kang, G.S.;Park, M.J.;Chang, M.J.;Kim, B.M.;Ko, D.C.
    • Transactions of Materials Processing
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    • v.25 no.4
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    • pp.268-274
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    • 2016
  • In the automotive seat industry, the use of a fine blanking press is important for manufacturing of high precision products. Among them, a gear part which is a main component of an automotive seat recliner is generally manufactured by fine blanking press. However, the use of conventional mechanical press has been increasing in manufacture of gear part because of low productivity of fine blanking press. In this study, new forming process is suggested to fabricate the gear part with high precision by using mechanical press. The effect of flow restriction die (FRD) which has the restriction of blank edge on dimensional accuracy is investigated by FE-analysis. FE-analysis results for different conditions of FRD indicated that FRD has high dimensional accuracy with the lowest roll-over and the highest perpendicularity of gear part. After application to fabrication of the gear part using mechanical press, the measured dimensional characteristic was compared with the required specification of final product. In addition, results of the performance test showed that the product fabricated by developed process satisfied the required strength and durability. The results show that the suggested forming process by using FRD and mechanical press can replace fine blanking on the viewpoint of dimensional accuracy and productivity.

A Pantographic Study on the Mandibular Movements in Patients with Temporomandibular Joint Sounds (Pantograph를 이용한 악관절잡음자의 하악운동에 관한 연구)

  • Lee, Pung Ho;Han, Kyung Soo
    • Journal of Oral Medicine and Pain
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    • v.12 no.1
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    • pp.85-93
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    • 1987
  • The author studied on the effect of TMJ sounds to the patterns and ranges of mandibular border movements in horizontal plane with Pantograph (Denar Corp.). For study, 19 patients with TMJ sounds only and 16 students with no TM disorder were selected and classified as experimental group and control group, respectively. The subject performed right lateral movement, left lateral movement, and forward movement. Each movement were performed 3 times and the movement trajectory obtained with mechanical pantograph were observed for accordance of centric relation position, reproducibility and/or restriction of lateral movement paths, deviation of protrusive path in anterior table, restriction of protrusive condylar movement path in posterior horizontal table, presence of Fisher angle in posterior vertical table. And pantographic reproducibility Index (PRI) were obtained with pantronic by the same movement method as in the mechanical pantograph record. The obtained results were as follows : 1. In experimental group, PRI scores in those who show accordance of centric relation position were 14.4, and were 26.53 in those who did not show accordance of centric relation position. However, the PRI scores of the two subgroups show no statistically significant difference in control group. Therefore, in experimental group, the capability of accordance of centric relation position affected largely the PRI scores than in control group. 2. Deviation of protrusive path was opposite to the affected side in experimental group, and was left side in control group. 3. Restriction side of condylar movement in protrusion was ipsilateral to the deviation side in experimental group, but in control group, restriction side was not related to the deviation side. 4. PRI scores in experimental group were 23.2 (moderate dysfunction category), and in control group, were 17.8 (slight dysfunction category). The PRI scores in control group, however, implies that the evaluation of temporomandibular disorders by the PRI scores only may be unreasonable.

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Analysis for the Squareness Measurement using Laser Interferometer (레이저 간섭계를 이용한 직각도 측정에 관한 분석)

  • Lee, Dong-Mok;Lee, Hoon-Hee;Yang, Seung-Han
    • Journal of the Korean Society for Precision Engineering
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    • v.29 no.8
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    • pp.863-872
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    • 2012
  • The squareness measurement of driving axes of a machine tool is very important to evaluate the performance of the machine. Laser interferometer measurement system is one of the most reliable equipment to measure the squareness. However, squareness measurement using laser system with an optical square result in restriction of straightness optics setup and Abbe's offset. This offset combines with angular errors during the motion of an axis to cause Abbe's error. In addition, the difficulty in optical square setup causes restriction of other optics and limitation of measurable range. In this paper, mathematical approaches are presented to eliminate the Abbe's error and to estimate squareness for full range by using the best fit of straightness data measured without an optical square. Experiments for squareness measurement of 3 axis machine tool were conducted and the proposed techniques were used for squareness evaluation with elimination of Abbe's error and squareness estimation for the full travel range.

Frequency Domain Properties of EALQR with Indefinite Weighting Matrix

  • Seo, Young-Bong;Park, Jae-Weon
    • Journal of Mechanical Science and Technology
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    • v.17 no.3
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    • pp.329-335
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    • 2003
  • EALQR (Linear Quadratic Regulate. design with Eigenstructure Assignment capability) has the capability of exact assignment of eigenstructure with the guaranteed margins of the LQR for MIMO (Multi-Input Multi-Output) systems. However, EALQR undergoes a restriction on the state-weighting matrix Q in LQR to be indefinite with respect to the region of allowable closedloop eigenvalues. The definiteness of the weighting matrix is closely related to the robustness property of a given system. In this paper, we derive a relation between the indefinite weighting matrix Q and the robustness property for EALQR. The modified frequency domain inequality, that could be guaranteed by EQLQR with an indefinite weighting matrix, is presented.

Performance Evaluation Test for Applying the Mechanical Development of the High-Strength Reinforcing Bars to the Nuclear Power Plant Structures (고강도철근 기계적정착의 원전구조물 적용을 위한 성능평가실험)

  • Lee, Byung-Soo;Bang, Chang-Joon;Lim, Sang-Joon;Kim, Seok-Cheol
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2012.11a
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    • pp.197-198
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    • 2012
  • If the mechanical development be applied to the Nuclear Power Plant Structures instead of the standard hook development, the problem of overcrowding re-bars in the anchorage zone can be solved and the construction quality of the concrete work will be improved. But there are some problems in applying it to the NPP structures because of the restriction on the re-bar yield strength and diameter. After the performance evaluation test for the mechanical development, we can develop the new design equation of the mechanical development length in order to solve the limitation and apply it to NPP structures.

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Investigation for the Restriction of the Stiffness and Mechanical Impedance of the Shipboard Floor and Foundation Considering Dynamic Stiffness of the Anti-Vibration Mount (방진 마운트의 동적 강성을 고려한 선체 바닥 및 받침대의 강성과 임피던스 규제에 대한 고찰)

  • Han, Hyung-Suk;Son, Yoon-Jun
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2009.04a
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    • pp.511-517
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    • 2009
  • The mechanical impedance and stiffness of the foundation of shipboard equipments and hulls supported by anti-vibration mount are very important so that the anti-vibration mount can accomplish its performance effectively. But, it is frequently argued how much stiffness and mechanical impedance are necessary for those foundations and hulls. In this research, it is discussed by evaluating the dynamic stiffness of the commercial anti-vibration mounts used in a naval vessel. Consequently, in this research, the minimum level of the mechanical impedance and stiffness of the foundation of shipboard equipments and hulls are suggested considering the dynamic stiffness of the mount which varies as frequency.

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Investigation for the Restriction of the Stiffness and Mechanical Impedance of the Shipboard Floor and Foundation Considering Dynamic Stiffness of the Anti-vibration Mount (방진 마운트의 동적 강성을 고려한 선체 바닥 및 받침대의 강성과 임피던스 규제에 대한 고찰)

  • Han, Hyung-Suk;Son, Yoon-Jun
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.19 no.3
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    • pp.320-326
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    • 2009
  • The mechanical impedance and stiffness of the foundation of shipboard equipments and hulls supported by anti-vibration mount are very important so that the anti-vibration mount can accomplish its performance effectively. But, it is frequently argued how much stiffness and mechanical impedance are necessary for those foundations and hulls. In this research, it is discussed by evaluating the dynamic stiffness of the commercial anti-vibration mounts used in a naval vessel. Consequently, in this research, the minimum level of the mechanical impedance and stiffness of the foundation of shipboard equipments and hulls are suggested considering the dynamic stiffness of the mount which varies as frequency.