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http://dx.doi.org/10.3795/KSME-A.2004.28.10.1598

Study on PDMS/Class Microthermostat Fabrication and Evaluation for Restriction Enzyme Reaction  

Jin Seok-Ho (한양대학교 대학원 정밀기계공학과)
Cho Yong-Jin (한양대학교 대학원 정밀기계공학과)
Ahn Yoomin (한양대학교 기계공학과, 마이크로바이오칩센터)
Publication Information
Transactions of the Korean Society of Mechanical Engineers A / v.28, no.10, 2004 , pp. 1598-1602 More about this Journal
Abstract
In this paper, we report a microthermostat using PDMS (poly-dimethylsiloxane) and glass. This PDMS/glass chip is able to maintain constant temperature that is necessary for restriction enzyme reaction. Since PDMS is the low-cost and the mass-producible material and has very good biochemical compatibility, PDMS chip has more benefit than general Si chip. Heater was made of Au wiring patterned on Pyrex glass. A reaction chamber has a capacity of about 3 ${mu}ell$. We performed a restriction enzyme reaction by using the fabricated microthermostat and conventional method. Then, with the electrophoresis, we made a comparison between the result from the micro reactor and the one from conventional method.
Keywords
Microthermostat; Restriction Enzyme Reaction; DNA;
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Times Cited By KSCI : 3  (Citation Analysis)
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