• KSBB Journal
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• v.8 no.5
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• pp.478-482
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• 1993

Perfusion culture was conducted in Celligen perfusion culture system using a self-constructed hybridoma cell and low serum medium. The culture system employed hollow fiber to separate cells from the culture broth. Maximum cell density of $2.1\times10^7$ ce11s/m1, 10 times higher than in batch culture, could be achieved. Concentration of monoclonal antibody (MAb) was 4 times higher and production rate at maximum feed rate was 9 times higher than in batch culture. Glucose concentration was very important for the cell growth and MAb production. When glucose concentration was below 1g/l, i. e. 0.5~0.9g/l, specific MAb production rate decreased but cell concentration still increased. As the glucose concentration goes above 1g/l, specific MAb production rate increased and remained at maximum value at more than 1.5g glucose/l. The maximum value of the specific Mab production rate was similar to that of batch culture.

• Korean Journal of Mathematics
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• v.13 no.2
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• pp.161-176
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• 2005

We consider a model of population dynamics whose mortality function is unbounded. We note that the regularity of the solution depends on the growth rate of the mortality near the maximum age. We propose Gauss-Legendre methods along the characteristics to approximate the solution when the solution is smooth enough. It is proven that the scheme is convergent at fourth-order rate in the maximum norm. We also propose discontinuous Galerkin finite element methods to approximate the solution which is not smooth enough. The stability of the method is discussed. Several numerical examples are presented.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.317-320
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• 2000

The optimal temperature and pH for both mycelial growth and exe-biopolymer production by Cordyceps millitaris in shake flask culture were found to be $20^{\circ}C$ and 6.0, respectively. Sucrose (4%) and corn steep powder (1%) were the most suitable carbon and nitrogen source for mycelial growth and exo-biopolymer production. The maximum specific growth rate $(0.142h^{-1})$ was achieved when sucrose was used as the sole carbon source. Exo-biopolymer production was increased with the increase in C/N molar ratio concentration, probably due to the facilitated carbon uptake. Under the optimal culture conditions, the maximum mycelial growth exe-biopolymer concentration were reached to around 13.3 g dry cell weigh/l and 3.33 g/l, respectively.

• Polymer(Korea)
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• v.25 no.6
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• pp.840-847
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• 2001

The crystallization characteristics of metallocene linear low density polyethylene (m-LLDPE)/linear low density polyethylene (LLDPE) blends were investigated. The effect of blending on the induction time for crystallization, spherulites growth rate, and maximum size of spherulites was mainly considered in this study. The formation of separate crystal which is well known crystallization behavior in LLDPE/LDPE blend was not found in m-LLDPE/LLDPE blends. The blending m-LLDPE to LLDPE caused the dramatic decrease in the induction time of m-LLDPE/LLDPE blends but it seems that the blend composition shows less effect on the induction time. Lower branching number in m-LLDPE resulted in the increasing of spherulites growth rate and the maximum size of spherulites is depend upon both the induction time and spherulites growth rate of LLDPE component affected by m-LLDPE.

• Journal of Microbiology and Biotechnology
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• v.21 no.6
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• pp.652-658
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• 2011

The growth kinetics of Streptomyces noursei NRRL 5126 was investigated under different aeration and agitation combinations in a 5.0 l stirred tank fermenter. Poly-${\varepsilon}$-lysine biosynthesis, cell mass formation, and glycerol utilization rates were affected markedly by both aeration and agitation. An agitation speed of 300 rpm and aeration rate at 2.0 vvm supported better yields of 1,622.81 mg/l with highest specific productivity of 15 mg/l.h. Fermentation kinetics performed under different aeration and agitation conditions showed poly- ${\varepsilon}$-lysine fermentation to be a growth-associated production. A constant DO at 40% in the growth phase and 20% in the production phase increased the poly-${\varepsilon}$-lysine yield as well as cell mass to their maximum values of 1,992.35 mg/l and 20.73 g/l, respectively. The oxygen transfer rate (OTR), oxygen utilization rate (OUR), and specific oxygen uptake rates ($qO_2$) in the fermentation broth increased in the growth phase and remained unchanged in the stationary phase.

• Journal of the Korean Society for Precision Engineering
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• v.8 no.2
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• pp.27-35
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• 1991

We propose the crack growth rate equation which applied over three regions (threshold region, stable region, unstable region) of fatigue crack propagation. Constant stress amplitude fatigue tests are conducted for four materials under three stress ratios of R=0.05, R=0.2 and R=0.4. Materials which have different mechanical properties i.e. stainless steel, low carbon steel, medium carbon steel and aluminum alloy are used. The fatigue crack growth rate equation is given by $da/dN={\beta} (1-R)^{\delta}\({\DELTA}K-{\DELTA}K_t)^{\alpha} / (K_{cf}-K_{max})$${\alpha}, {\beta}$ , and ${\delta}$ are constants, and ${\Delta}K_t$ is stress intensity factor range at low ${\Delta}K$ region. The constants are obtained from nonlinear least square method. $K_{ef}$is critical fatigue stress intensity factor. The relation between half crack length and number of cycles obtained by integrating the crack growth rate equation is in agreement with the experimental data. It is also experimented with constant maximum stress and decreasing stress ratios, and the fatigue growth rate of each material is in accord with the proposed equation.

• Journal of Ginseng Research
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• v.19 no.2
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• pp.181-187
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• 1995

Cylindvocarpon destmtalns isolate CY-92-01, pathogen of root-rot of Panax ginseng showed t the maximum mycelial growth on the Czapek solution agar among the thirteen kinds of media. Five isolates (Isolate CY-92-01, CY-92-03, CY-92-07, CY-94-01, CY-94-02) of C. destructan from various growth stages of p. ginseng recovered from several geographical sites also showed maximum growth in the Czapek-Dox broth compared with potato dextrose broth and V-8 juice broth. Rapid growth rate was maintained until 12 days after inoculation on the Czapek-Dox broth and mycelial weight was somewhat constant until 20 days. After 30 days of incubation, the mycelial weight began to decrease. The fungal growth occurred from 5$^{\circ}C$ to $25^{\circ}C$ and optimum temperature for growth was 2$0^{\circ}C$. Mycelial weight orderly decreased at 15, 25, 10, and 5$^{\circ}C$. Quantitative measurement was impossible at 5$^{\circ}C$. No fungal growth was occurred at the temperature higher than 3$0^{\circ}C$. Growth was observed at all tested pH ranges from 2.8 to 8.0. Optimum pH for growth was 4.0~5.0 followed by pH 3.3~3.5 and 5.4~6.0. The least growth occurred at pH 2.8.

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.55 no.6
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• pp.328-333
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• 2006

All the plants on earth live under an electric and magnetic field because the earth is a magnet and there is an electric field between the charged cloud and the ground. It has been reported that electromagnetic fields influence both the activation of ions and polarization of dipoles in living cells of seeds and plants, though the mechanism of these actions is still poorly understood. In this paper, the effects of the electric and magnetic fields and exposure times to the germination of several vegetable seeds and its early growth have been investigated experimentally to find out the feasibility of a plant factory for mass production of clean and unpolluted vegetables. The germination rate and the growth rate of some seeds under the fields exposed were analysed and compared with those of unexposed ones. It is found that the germination rate and its early growth rate of exposed seeds under the fields were accelerated about 1.1-1.4 and 1.7-2.2 times in maximum compared with those of unexposed ones. But, however, an inhibitory effect on germination and plant early growth were shown in the case of the higher electric and magnetic fields.

• Fire Science and Engineering
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• v.32 no.1
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• pp.16-23
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• 2018

In a closed-compartment with various configurations, the correlation that can predict the maximum heat release rate (HRR) with the changes in internal volume and fire growth rate was investigated numerically. The volume of the compartment was controlled by varying the length ratio based on the bottom surface shape of the ISO 9705 fire room, where the ceiling height was fixed to 2.4 m. As a main result, the effect of a change in ceiling height on the maximum HRR was examined by a comparison with a previous study that considered the change in ceiling height. In addition, a more generalized correlation equation was proposed that could predict the maximum HRR in closed-compartments regardless of the changes in ceiling height. This correlation had an average error of 7% and a maximum error of 19% for various fire growth rates when compared with the numerical results. Finally, the applicability of the proposed correlation to representative fire compartments applied to the domestic performance-based design (PBD) was examined. These results are expected to provide useful information on predicting the maximum HRR caused by flashover in closed-compartments as well as the input information required in a fire simulation.

• Transactions of the Korean Society of Mechanical Engineers
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• v.19 no.10
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• pp.2657-2666
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• 1995

In this study, the effects of design factors of finned-tube heat exchanger, such as fin spacing and fin array on the frost growth and heat exchanger performance are investigated under a frosting condition. The results show that the amount of frost, frost density and blockage ratio of air flow passage increase with decreasing fin spacing. Heat transfer rate increases momentarily at the initial stage of frosting and then decreases. After that heat transfer rate continues to increase again to reach a maximum value and then decreases dramatically. It is shown that the time required for heat transfer rate to reach a maximum value becomes shorter with decreasing fin spacing, and after a maximum value, heat transfer rate decreases very fast. The maximum allowable blockage ratio is introduced to determine the operation limit of a finned-tube heat exchanger operating under frosting condition and is obtained as a function of fin spacing. It is also shown that heat transfer rate of heat exchanger with staggered fin array increases about 17% and the amount of pressure drop of air increases about 1~2 mmH$_{2}$O, compared with those of in-line type heat exchanger under frosting condition.