• Title/Summary/Keyword: Maximum growth rate

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Nutrient Uptake and Growth Kinetics of Chattonella antiqua (Hada) Ono (Raphidophyceae) Isolated from Korea

  • Seo, Kyung-Suk;Lee, Chang-Kyu
    • ALGAE
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    • v.22 no.3
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    • pp.235-240
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    • 2007
  • The red tide-causing flagellate Chattonella anticfua can cause mass fish kills by their clogging in fish gills. Thisstudy examined the nutrient requirements of C. antiqua isolated from Korea. C. anticfua displayed maximum growthat the day five, followed by a decrease in cell density. Nitrate and nitrite were the preferred nitrogen sources, alonewith adenosine diphosphate for phosphorus compounds. In medium that contained ammonium, a significantdecrease in cell density was observed. Half-saturation constants, Ks, calculated from the maximum growth ratewere 4.94 U|M for NC>3 and 0.79 flM for P04. The growth of C. antiqua was not within the function of the N:P ratio (RU= 0.29). With an N:P ratio as low as 10, the increase in cell density was apparent, with a higher division rate. At lev-els above 50 fiM of NaNOg or 8 ;uM of NaHUPCU, the growth rates were somewhat decreased. Phosphate was thelimiting factor for C. antiqua growth since the starvation of phosphate had brought about a rapid decrease in celldensity in semi-continuous culture. Studies about the temporal modification of the efficiency of nitrate or phosphateuptake may be necessary to explain the bloom dynamics of C. antiaua.

Analysis of Temperature Effects on Microbial Growth Parameters and Estimation of Food Shelf Life with Confidence Band

  • Park, Jin-Pyo;Lee, Dong-Sun
    • Preventive Nutrition and Food Science
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    • v.13 no.2
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    • pp.104-111
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    • 2008
  • As a way to account for the variability of the primary model parameters in the secondary modeling of microbial growth, three different regression approaches were compared in determining the confidence interval of the temperature-dependent primary model parameters and the estimated microbial growth during storage: bootstrapped regression with all the individual primary model parameter values; bootstrapped regression with average values at each temperature; and simple regression with regression lines of 2.5% and 97.5% percentile values. Temperature dependences of converted parameters (log $q_o$, ${\mu}_{max}^{1/2}$, log $N_{max}$) of hypothetical initial physiological state, maximum specific growth rate, and maximum cell density in Baranyi's model were subjected to the regression by quadratic, linear, and linear function, respectively. With an advantage of extracting the primary model parameters instantaneously at any temperature by using mathematical functions, regression lines of 2.5% and 97.5% percentile values were capable of accounting for variation in experimental data of microbial growth under constant and fluctuating temperature conditions.

Effect of Gamma Rays on the Growth Performance of Bangladesh Clone Tea

  • Ali, M. Aslam;Samad, M. A.;Amin, M. K.
    • Korean Journal of Environmental Agriculture
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    • v.24 no.1
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    • pp.66-70
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    • 2005
  • The experiment was carried out to investigate the effects of gamma radiation on the early growth performance and physiological traits of BT2 clone tea, the most promising cultivar released by Bangladesh Tea Research Institute. The fresh shoot cuttings were irradiated with seven different levels of gamma radiation such as 0, 10, 20, 30, 40, 50 and 60 Gy from Cobalt 60Co source (Dept. of PlantBreeding, Bangladesh Institute of Nuclear Agriculture). Thereafter, the irradiated shoot cuttings were planted in polythene bags and kept under natural conditions. It was observed that callusing was initiated from 8th weeks after placement of tea shoot cuttings in the polythene bags and completed by 12th weeks. The morphological growth of tea shoot cuttings were recorded under varying levels of gamma radiation and growth stages. It was observed that the number of leaves, number of primary branches, base diameter, root length and total leaf area per plant significantly increased with the progress of time and increasing levels of gamma radiation, however, the plant height showed decreasing trend with the increasing levels of gamma radiation, which could be due to the change in chromosomal structure and genetic makeup. After 56 weeks of planting, the plant height, the number of leaves and primary branches per plant, base diameter, root length and total leaf area per plant recorded were 65.70 cm, 30.67, 7.33, 1.48 cm, 23.50 cm, and 1250.67 cm2 per plant respectively under the radiation level 60 Gy, whereas the corresponding figures of the above parameters at the control treatment were 76.21 cm, 18.33, 3.67, 0.92 cm, 17.75 cm and 778.33 cm2 per plant, respectively. A significant relationship was observed among the physiological growth parameters with the increasing levels of gamma radiation. The total dry matter gain, leaf area index, absolute growth rate and relative growth rate were significantly influenced with the rising levels of gamma radiation (up to 60 Gy), whereas the net assimilation rate of individual tea plant non-significantly responded as compared to those of control treatment. Finally after 56 weeks of planting, the maximum total dry weight gain, leaf area index, absolute growth rate, relative growth rate and net assimilation rate recorded under 60 Gay radiation level were 40.25 g/plant/week, 4.25, 1.18 g/week, 0.0621g/g/week and 17.07 g/m2/week respectively.

Medium Recycle Process for the Production of scu-PA from Serum Free Medium (무혈청 배지로부터 scu-PA의 생산을 위한 배지의 재사용 공정에 관한 연구)

  • 김영남;박경유
    • KSBB Journal
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    • v.8 no.1
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    • pp.17-22
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    • 1993
  • $1.96{\times}10^{-5}$(IU/cell/hr) of specific scu-PA production rate was obtained from HEK cells in maintaining ca. $8{\times}10^{5}$(cells/ml) of maximum roll density at 10(ml/hr) of perfusion rate with recycling 20% serum free conditioned media. It can be compared to $4{\times}10^{6}$(cells/ml) of maximum cell density and $4.56{\times}10^{-4}$(IU/cell/hr) of specific production rate in cultivating cells with 1% serum containing medium. Thc conversion ratio of scu-PA to tc-UK increased up to 55% as the recycling ratio increased; however, recycling the used medium seemed to have least negative effect on cell growth. It also showed that the recycling process had definitive advantage of using serum free medium in perfusion cultivation of HEK cell line.

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Estimation of Protein Deposition Rate of Growing-Finishing Pigs Reared in Commercial Conditions in Korea

  • Kim, J.H.;Sohn, K.S.;Hynn, Y.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.8
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    • pp.1147-1153
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    • 2000
  • A total of 9,540 pigs were evaluated for their growth performance to provide information on the development of different feeding strategies to support maximum rate of protein deposition (PD). Large variations in growth performance and protein deposition rate were found in the population used in this study (ADG from 701 to 974 g/day; ADFI from 1,726 to 2,498 g/day; Feed/gain from 2.10 to 2.90; Backfat thickness from 12.4 to 20.5 mm and PD rate from 103 to 153 g/day). It was found that ADG was positively correlated to PD ($R^2=0.9362$, p<0.0001) while FCR was negatively correlated to PD ($R^2=0.4031$, p<0.0001). Backfat thickness was negatively correlated to PD ($R^2=0.7024$, p<0.0001) and to ADG ($R^2=0.5096$, p<0.0001). The estimated lysine requirement based on PD rate also showed large variation (12.37 to 18.38 g/day true ileal digestible lysine on average between 25 and 100 kg), thus strongly indicated the need of separate feeding strategies for each group of pigs. When pigs were divided into three categories according to estimated whole body PD rate, the group of pigs with the highest PD rate grew faster by 6.3 and 13.9% than pigs with intermediate and low PD rate, respectively. Feed utilization was also more efficient in pigs with a high PD rate. It appeared that pigs with high PD rate maintained higher PD rate especially in the later stage of their life. Pigs with high PD rate require an extra amount of 1.2 and 2.4 g/true digestible lysine per day and 0.4 and 0.8% more lysine in the diet than pigs with intermediate and low PD rate during the growing-finishing period respectively. Results of this study suggest that there is a need for separate feeding strategies for individual group of pigs with different PD rate. It should be noted that average value for each group presented in this report is not the adequate amount for an animals potential for maximum PD rate. With recent development in growth modeling and access to computer technologies to facilitate computation, pork producers can easily estimate pigs protein deposition rate and thus can make their own feeding strategies.

Effects of inert gas (Ne) on thermal convection of mercurous chloride system of $Hg_2Cl_2$ and Ne during physical vapor transport

  • Choi, Jeong-Gil;Lee, Kyong-Hwan;Kim, Geug-Tae
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.18 no.6
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    • pp.225-231
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    • 2008
  • For an aspect ratio (transport length-to-width) of 5, Pr=1.13, Le=1.91, Pe=4.3, Cv=1.01, $P_B=20\;Torr$, the effects of addition of inert gas Ne on thermally buoyancy-driven convection ($Gr=2.44{\times}10^3$) are numerically investigated for further understanding and insight into essence of transport phenomena in two dimensional horizontal enclosures. For $10K{\leq}{\Delta}T{\leq}50\;K$, the crystal growth rate increases from 10 K up to 20 K, and then is slowly decreased until ${\Delat}T=50\;K$, which is likely to be due to the effects of thermo-physical properties stronger than the temperature gradient corresponding to driving force for thermal convection. The dimensional maximum velocity gratitude reflecting the intensity of thermal convection is directly and linearly proportional to the temperature difference between the source and crystal regions. The rate is first order-exponentially decreased for $2{\leq}Ar{\leq}5$. This is related to the finding that the effects of side walls tend to stabilize convection in the growth reactor. In addition, the rate is first order exponentially decayed for $10{\leq}P_B{\leq}200\;Torr$.

The Effect of Residual Stress on Stress Intensity Factor and Fatigue Crack Growth Rate (잔류응력이 응력세기계수와 피로균열성장율에 미치는 영향)

  • Kang-Yong,Lee;Hong-Key,Kim
    • Bulletin of the Society of Naval Architects of Korea
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    • v.21 no.1
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    • pp.43-47
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    • 1984
  • The purpose of this paper is to investigate theoretically the effect of residual stress due to welding in stress intensity factor of a plate containing the Model I Crack in different crack size and location, and on fatigue crack growth rate. The initiation of crack is found to be possible only in the region of tensile residual stress. The most dangerous crack has the values of d/b and a/b equal to about 0.6 and 1.0, respectively, where d/b is the ratio of distance from the crack to welding bead and the width of tensile residual stress region and a/b is the ratio of crack length and tensile residual stress region. The crack perpendicular to and on the line of welding bead and with a/b equal to about 0.6 has maximum stress intensity factor. The theoretical fatigue crack growth rate under residual stress and applied stress, which is obtained from Forman's Law by stress superposition, is relatively in good agreement with Glinka's[8] experimental value. The fatigue crack growth is shown to be retarded due to residual stress distribution.

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Kinetics of producing ${\beta}$-carotene from Dunaliella salina by Light Limited Turbidostat Cultivation (Dunaliella salina 의 광 제한 현탁 연속배양에 의한 ${\beta}$-carotene 의 생산)

  • Park, Young-Shik;You, Ho-Keum;Ohh, Shang-Jip;Lee, Hyeon-Yong
    • Microbiology and Biotechnology Letters
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    • v.21 no.4
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    • pp.342-347
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    • 1993
  • It was proved that the cell growth followed a photo-inhibition model in light-limited turbidostat cultivation, having 1.06 (1/h) of maximum specific growth rate and 0.00094(kcal/$cm^2$/h) and 0.063 (kcal/$cm^2$/h) as half saturation and light inhibition constants, repectively. ${\beta}$-carotene production showed a growth related porcess. And the activation energy of Dunaliella salina was roughly estimated as 12.36 (kcal/mole) in employing Arrhenius relationship. It should also point out that relatively much porduction of ${\beta}$-carotene was observed at hight light intensity with yieding 1.04 (mg-carotene/g-dry cell/day) of specific product production rate while the cell growth was decreased. The optimal conditions of producing ${\beta}$-carotene in turbiodostat cultivation were as follows: $7.5{\times}10^{-3}$(kcal/$cm^2$/h)of light intensity, 2 (mM) and 50(mM) of nitrate and sodium bicarbonate concentrations and 100(ml/h) of $CO_2$ flow rate.

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Mycelial Growth Enhancement in Liquid Cultivation of Hericium erinaceus

  • Kim, Min-Seok;Seo, Jeong-Sik;Hong, Eok-Gi
    • 한국생물공학회:학술대회논문집
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    • 2003.04a
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    • pp.236-239
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    • 2003
  • Erinacine which is produced from Hericium erinaceus mycelia is known to have a stimulating activity of nerve growth factor(NGF) synthesis. Thus, this work was concentrated on the maximum production of Hericium erinaceus mycelia. In order to maximize cell growth, the cultivation was performed with varying the agitation rate and applying the additional medium. As the results, the mycelium concentration was 24.2g/L.

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Thermoelastic Finite Element Analysis of Double horizontal Subsurface Cracks Due to Sliding Surface Traction (마찰열을 고려한 미끄럼 접촉시 내부 복수 수평균열 전파해석)

  • 이진영;김석삼;채영훈
    • Tribology and Lubricants
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    • v.18 no.3
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    • pp.219-227
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    • 2002
  • A linear elastic fracture mechanics analysis of double subsurface cracks propagation in a half-space subjected to moving thermomechanical surface traction was performed using the finite element method. The effect of frictional heat at the sliding surface on the crack growth behavior is analyzed in terms of the thermal load and peclet number. The crack propagation direction is predicted in light of the magnitudes of the maximum shear and tensile stress intensity factor ranges. When moving thermomechanical surface traction exists, subsurface horizontal cracks are propagation in-plane crack growth rate at the beginning but they are propagation out-of-plane crack growth rate by the frictional heat which is occurrence by the repeated sliding contact.