• 대한의용생체공학회:의공학회지
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• 제41권2호
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• pp.67-74
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• 2020

Brain tumors or gliomas are fatal cancer species with high recurrence rates due to their strong invasiveness. Therefore, the goal of surgery is complete tumor resection. However, the surgery is difficult to distinguish the border because tumors and blood vessels have the same color tone and shape. The fluorescein sodium is used as a fluorescence contrast agent for boundary separation. When the external light source is irradiated, yellow fluorescence is expressed in the tumor, which helps distinguish between blood vessels and tumor boundaries. But, the fluorescence expression of fluorescence sodium depends on the concentration of fluorescein sodium and such analytical data is insufficient. The unclear fluorescence can obscure the boundaries between blood vessels and tumors. In addition, reduce the efficiency of fluorescence sodium use. This paper proposes a protocol of concentration range for fluorescence expression conditions. Fluorescent expression was observed using a near-infrared (NIR) color camera with corresponding dilution using normal saline in 1 ml microtube. The flunoresence emission density range is 1.00 mM to 0.15 mM. The fluorescence emission begin to 1.00 mM and the 0.15 mM discolor. The discolor is difficult to fluorescence emission condition obserbation. Thus, the maximum density range of the bright fluoresecein is 0.15 mM to 0.30 mM. When the concentration range of fluorescein sodium is analyzed based on the gradient of fluorescence expression and the power measurement, the brightest fluorescence is expected to facilitate the complete resection of the tumor. For the concentration range protocol, setting concentration ranges and analyzing fluorescence expression image according to saturation and brightness to find optimal fluorescence concentration are important. Concentration range protocols for fluorescence expression conditions can be used to find optimal concentrations of substances whose expression pattern varies with concentration ranges. This study is expected to be helpful in the boundary classification and resection of brain tumors and glioma.

• 한국식품과학회지
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• 제4권3호
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• pp.200-205
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• 1972

Candida tropicalis KIST 351을 $2.5{\ell}$ 및 $28{\ell}$ 발효조에시 배양하여 석유 유분별자화능, 석유탄화수소기질의 즉차적 유가배양(流加培養), 폐배지(spent medium)의 재사용 및 연속배양 등 배양기술적인 기초문제를 검토한 바 그 개요는 다음과 같다. (1) 각종 경유유분(LGO, HGO, VGO, Diesel oil, SP(E), HGO-wax, L/M-wax)에 있어저 균체농도는 $11{\sim}15g/{\ell}$, 경유에 대한 수율은 $10{\sim}12%$, 소비된 n-paraffin에 대한 수율은 $77{\sim}82%$였다. (2) 동 균주를 유가배양 함으로써 균체들 배지에 대하여 $18.9g/{\ell}$, 경유에 대한 수율을 13%로 향상시킬 수 있었다. (3) 폐배지는 탄화수소를 유화시키고 증식을 촉친하는 효과를 가지고 있었으며 $Mg^{++}$를 보충하지 않었을 때는 20%, 보충하였을 때는 50%까지 회수 재 사용 할 수 있었다. (4) 균체생산을 위한 연속배양의 최적희석율은 $D=0.1{\sim}0.125\;hr^{-1}$의 범위내에 있으며, 이때의 균체농도는 약 $8g/{\ell}$였고, $D=0.125\;hr^{-1}$에서의 균체생산속도는 $1.0\;g/{\ell}/hr$로써 최대치에 달하였다. 소비된 경유 중의 n-paraffin에 대한 균체수율은 $D=0.1\;hr^{-1}$에서 0.94로써 최대치를 보였다. 그러나 희석율 $0.1\;hr^{-1}$에서 공급된 n-paraffin의 약 24%가 기질로써 소비 되지 않었으므로 이에 대한 적절한 대책이 요구된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.46-46
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• 2019

The experiments were performed in the Jinan (elevation: 300 meters above sea level), Jeollabuk-do. Seedlings (n = 63 per $3.3m^2$) of ginseng cultivar (Cheonpung, Yeonpung) were planted on April 10, 2015. Shading material of plastic film house was blue-white film. Before the Planting seedling, silicate (3 kg/10 a) or chitosan (40 kg/10 a) was fertilized and foliar sprayed on the leaves 1000 times dilution solution once a month from May to September every year. The growth results of 5-year old ginseng surveyed in 2018 are as follows. The average air temperature in the plastic film house was the highest at $26.6^{\circ}C$ and $26.5^{\circ}C$ in July and August, respectively, and the highest temperature was $40.5^{\circ}C$ in July. The maximum daily temperature of $35^{\circ}C$ or more was 30 days, with the average soil temperature being $24.9^{\circ}C$ in August. The chemical properties of the test soil are as follows. pH was 6.4~6.9 level and EC was 0.35~0.46 dS/m. The organic matter content was 33.5~41.4 g/kg, and available-P content was 251.9~306.8 mg/kg. Exchangeable cations contents, such as K, Ca and Mg were all the appropriate ranges. The soil microbial density surveyed by the dilution plate method was 10~50 times higher than that of control (Non-treatment) and actinomycete density was 3~6 times higher. Pathogens of the genus Fusarium by Metagenome analysis decreased 91.3% and 68.2% respectively in the foliar sprayed of chitosan and soluble-silicate. The light intensity (PAR) in the blue-white film plastic film house gradually increased until July and then decereased, with the average of light intensity in March-October was $120.3umol/m^2/s$. The growth of aerial parts such as plant height and stem length was better than non-sprayed group in silicate or chitosan treatments and Yeonpung cultivar was superior to the Cheonpung cultivar. The SPAD value was higher in Yeonpung cultivar foliar sprayed with soluble-silicate. The growth of underground parts such as root length and taproot length were better in chitosan and soluble-silicate treatment than control, especially in Yeonpung cultivar foliar sprayed with chitosan was good in taproot length and taproot diameter, and fresh weight of root was 60.1 g. Ginsenoside contents were 24.9 mg/g and 22.4 mg/g, in the Cheonpung cultivar foliar sprayed with soluble-silicate or chitosan respectively, 28% and 15% higher than control (19.5 mg/g). The incidence of disease by Alteraria panax and Botrytis cinerea was 3~9% and 4~9%, respectively. High temperature damage rate was 3~5%.

• 생태와환경
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• 제38권1호통권110호
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• pp.54-62
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• 2005

본 연구는 하절기 집중강우가 하천의 부영양화도, 이온변화, 산소요구량에 영향 평가로서 수질 변수들 간의 상호관계를 분석하였다. 용존산소(DO) 농도는 수온과 역상관 관계 (r= -0.99, p<0.001)를 보였다. 대부분 수질변이는 7 ${\sim}$ 8월에 발생 하였으며, 이들의 대부분은 하절기 집중강우와 직접적인 연관성을 보였다. pH의 경우 6.5 이하의 최소값은 최대 강우를 보인 8월에 관측되었는데, 이는 강우에 의한 수소이온농도의 희석현상에 의한 것으로 사료되었다. 전기전도도 또한 강우분포를 반영하였다. 즉, EC 값은 다른 계절보다 하절기에 좀 더 높은 값을 보였으며, 강우와의 직접적 연관성을 보였다. 연구결과에 따르면, 이온희석 현상은 강우 전의 하절기에는 높았지만, 집중강우 후 짧게는 4 ${\sim}$ 5일 길게는 1 ${\sim}$ 2주 후에 이온이 희석되는 것으로 나타나 강우와 하천수의 이온농도사이에 뚜렷한 반응시간의 지체현상을 보였다. COD의 계절적 변화 페턴은 BOD와 유사한(r= 0.55, p<0.001) 양상을 보였다. 총질소 (TN)는 총인 (TP)에 비해 변이 폭이 적었으며, 3월의 갈수기에 최소값을 보였다. 대조적으로, 총인 유입은 하절기 몬순동안에 주로 발생하였고, 총부유물(TSS)과 유사한 계절 변화 양상을 보여인(P)의 증가가 수계에서 유발되는 무기성부유물과 밀접하게 연관성을 갖는 것으로 나타났다. 총인: 총질소의 무게비는 질소 변이 (r= -0.21, p<0.01)보다는 총인(r= -0.51, p<0.01)의 변이에 의해 결정되었으며, 총인이 제한 요인으로 작용할 것으로 사료되었다. 본 계류형 하천에서 수질을 조절하는 1차 요인은 강우시기 및 강고로 사료 되었으며, 최대 변이는 하절기의 첨두강우와 일치하였다.

• Restorative Dentistry and Endodontics
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• 제38권1호
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• pp.36-42
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• 2013

Objectives: This study evaluated the antibacterial effect and mechanical properties of composite resins ($L_{CR}$, $M_{CR}$, $H_{CR}$) incorporating chitosan with three different molecular weights (L, Low; M, Medium; H, High). Materials and Methods: Streptococcus (S). mutans 100 mL and each chitosan powder were inoculated in sterilized 10 mL Brain-Heart Infusion (BHI) solution, and was centrifuged for 12 hr. Absorbance of the supernatent was measured at $OD_{660}$ to estimate the antibacterial activities of chitosan. After S. mutans was inoculated in the disc shaped chitosan-containing composite resins, the disc was cleansed with BHI and diluted with serial dilution method. S. mutans was spread on Mitis-salivarius bacitracin agar. After then, colony forming unit (CFU) was measured to verify the inhibitory effect on S. mutans biofilm. To ascertain the effect on the mechanical properties of composite resin, 3-point bending and Vickers hardness tests were done after 1 and 3 wk water storage, respectively. Using 2-way analysis of variance (ANOVA) and Scheffe test, statistical analysis was done with 95% significance level. Results: All chitosan powder showed inhibition effect against S. mutans. CFU number in chitosan-containing composite resins was smaller than that of control resin without chitosan. The chitosan containing composite resins did not show any significant difference in flexural strength and Vickers hardness in comparison with the control resin. However, the composite resin, $M_{CR}$ showed a slightly decreased flexural strength and the maximum load than those of control and the other composite resins $H_{CR}$ and $L_{CR}$. Conclusions: $L_{CR}$ and $H_{CR}$ would be recommended as a feasible antibacterial restorative due to its antibacterial nature and mechanical properties.

• 한국미생물·생명공학회지
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• 제8권1호
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• pp.55-60
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• 1980

높은 유산생성 능력을 가진 lactobacillus bulgaricus NLS-4주를 대창 배양하기 위하여 배양조건을 개선하였다. 이제까지 보고된 많은 배지중에서 7개를 선정하고 흔히 사용되고 있는 11% 탈지유 배지를 대조군으로 하여 사용 균주의 증식과 유산 생성능을 비교한 결과 TIP배지를 최종 선정하였다. 이 배지를 이용하여 38$^{\circ}C$에서 통기없이 200rpm의 속도로 교반해 주면서 IN KOH용액을 사용하여 PH를 6.5로 자동 조절해준 결과 18시간 동안의 회분배양에서 얻은 균체수는 생균수로 $m\ell$ 당 1.0$\times$$10^{9}$ 이었으며 이를 다시 연속 배양한 결과 희석 배율 D=0.17$hr^{-1}$에서 $m\ell$당 총 2.3$\times$$10^{9}$ 의 균체를 얻을 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제13권1호
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• pp.29-34
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• 2003

A Pseudomonas sp. strain NGK1 (NCIM 5120) capable of utilizing salicylate was immobilized in alginate and polyurethane foam (PUF). The degradation rate of salicylate by freely suspended cells was compared with the degradation rate by immobilized cells. In an initial 20 and 40 mM salicylate, free cells ($2{\times}10^{11}\;cfu\;ml^{-1}$) degraded to 16 and 14 mM, alginate-entrapped cells degraded to 18 and 26 mM, and PUF-entrapped cells degraded to 20 and 32 mM salicylate, respectively, in batch cultures. The alginate-and PUF-entrapped cells were used in repeated batch and continuous culture systems. The efficiency of both the immobilized systems f3r the degradation of salicylate was compared. It has been observed that the PUF-entrapped cells could be reused for more than 20 cycles whereas alginate-entrapped cells could be reused for a maximum of only 12 cycles, after which a decrease in degradation rat was observed with the initial 20 and 40 mM salicylate. The continuous degradation of sallcylate by freely suspended cells showed a negligible degradation rate of salicylate when compared with immobilized cells. With the immobilized cells in both alginate and polyurethane foam, the degradation rate increased with an increase in the dilution rate up to $2\;h^{-1}$ for 20 mM, and $1.5\;h^{-1}$ for 40 mM salicylate. The results revealed that PUF-entrapped cells were more efficient for the degradation of salicylate than alginate-entrapped cells and freely suspended cells.

• 한국산학기술학회논문지
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• 제15권6호
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• pp.3370-3375
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• 2014

MILD(Moderate and Intense Low Oxygen Dilution) 연소는 열효율 향상과 유해배출가스 저감의 상반된 관계를 해결하기 위한 하나의 각광받는 기술이다. 연소가스의 재순환을 이용하여 고온 연소시에 질소산화물을 낮게 유지함과 동시에 연소로 내부온도를 균일화함으로써 열효율을 향상시킬 수 있는 기술이다. 본 연구는 실험실 규모의 노에서 원추형 MILD 연소기의 연소특성을 나타내고 있다. 연구의 조건은 공기의 유량은 일정하게 하면서 가스 연료 유량을 변화시켜 당량비를 변화시켰다. 이 결과 노 내에서 MILD 연소영역이 잘 구현되었고, 당량비 0.69~0.83의 범위에 걸쳐서 노(爐)내에서의 온도와 배출가스의 농도가 각각 예측되었다. 이 당량비 구간에서 최고화염온도 영역과 주 반응영역에서의 온도차가 약 $300^{\circ}C$의 안정적인 화염 영역의 존재를 확인하였다.

• KSBB Journal
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• 제27권2호
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• pp.97-102
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• 2012

In the present study, the biomass productivity of two green microalgae (Chlorella sp. and Dunaliella salina DCCBC2) were assessed in a 12 L tubular photobioreactor under optimum culture conditions. In the batch culture optimization process, the Chlorella sp. biomass was obtained as 1.2 g/L under atmospheric air as a sole $CO_2$ source and other culture conditions as follows: light intensity, temperature, pH, $NH_4Cl$ and $K_2HPO_4$ were 100 ${\mu}E/m^2/s$, $27^{\circ}C$, 7.0, 20.0 mM and 2.0 mM, respectively. On the other hand, 2.9 g/L of D. salina DCCBC2 biomass production was observed under the following conditions: light intensity, temperature, pH, $KNO_3$ and $K_2HPO_4$were 80 ${\mu}E/m^2/s$, $27^{\circ}C$, 8.0, 3.0 mM and 0.025 mM, respectively. At 1% $CO_2$ supply to the reactor, the Chlorella sp. production was reached 1.53 g/L with 25% increment under the same operating conditions. In addition, the maximum D. salina DCCBC2 biomass was observed as 3.40 g/L at 3% $CO_2$ concentration. Based on the aforementioned optimized conditions, the dilution rate and maximal biomass productivity of Chlorella sp. and D. salina DCCBC2 in the continuous cultivation were 0.4/d and 0.6 g/L/d and 0.6/d and 1.5 g/L/d, respectively.

• 동력기계공학회지
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• 제19권3호
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• pp.16-21
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• 2015

Nitrous oxide($N_2O$) concentration in the atmosphere has been constantly increased by the human activities with industrial growth after the industrial revolution. One of factors to increase $N_2O$ concentration in the atmosphere is the $N_2O$ emission caused by the combustion of marine fuel. Especially, a sulfur component included in marine fuel oils is known as increasing the $N_2O$ formation in diesel combustion. Form this point of view, $N_2O$ emission from a ship is not negligible. On the other hand, Exhaust gas recirculation(EGR) that have thermal, chemical and dilution effect is effective method for reducing the NOx emission. In this study, an author investigated $N_2O$ reduction by using EGR on a direct injection diesel engine. The test engine was a 4-stroke diesel engine with maximum output of 12 kW at 2600rpm, and operating condition of the engine was a fixed load of 75%. The experimental oil was a blend-fuel that were adjusted with sulfur ratio of 3.5%, and EGR ratio of 0%, 10%, 20% and 30%. In conclusion, diesel fuel that contained 3.5% sulfur component increased $SO_2$ emission in exhaust gas, and increment of EGR ratio reduced NO emission. Moreover, $N_2O$ emission was decreased as over 50% at EGR ratio of 10% and reduced 100% at EGR ratio of 30% compared with $N_2O$ emission of 0% EGR ratio.