• 미생물학회지
• /
• 제28권3호
• /
• pp.188-191
• /
• 1990

The streptococcal plasmid pAM$\beta_{1}$ (erythromycin resistance)was transferred via conjugation from Streptococcus faecalis DS 5 to Lactobacillus casei YIT 9018 by a filter mating method. The transfer frequency depended greatly on the type, pore size and side(front or back) of membrane filter. Water passing through a membrane under reduced pressure induced very tight contact between the cells, increased the transconjugation frequency about 1.3 to 37-fold when Millipore membrane filter (0.45.$\mu$m, front side up) was used.

• Asian-Australasian Journal of Animal Sciences
• /
• 제5권3호
• /
• pp.469-473
• /
• 1992

Data on the birth weight, 8 week body weight and ewe body weight of an unselected random mating Romney Marsh sheep flock are analyzed to study the influence of various environmental factors. The average birth weight of lambs was 5.06 kg. Sex of lamb, birth type and dam age contributed significant variation in lamb birth weight (p < 0.05). Males were significantly heavier than females (p < 0.05) and singles were significantly heavier than multiples (p < 0.05) at birth. Birth weight of lamb increased with the progress of dams' age. The overall average 8 week body weight of lambs was 20.84 kg. Effect of birth weight, sex, birth type and dam age was significant on 8 week body weight of lamb. Eight week body weight increased with the increase of lamb birth weight (b=1.285 kg). Ewes' body weight taken before tupping was affected by ewes' age, year of performance and their weight at birth. It was concluded that performance data on lamb birth weight, 8 week body weight and ewe body weight should be corrected for the above relevantly significant environmental factors in any genetic calculation in the United Kingdom Romney March sheep.

• 한국임상수의학회지
• /
• 제7권2호
• /
• pp.521-525
• /
• 1990

Microspoum canis에 감염된 개 및 고양이와 직접 접촉한 경험이 있는 5명의 환자를 조사하여 동물기생체로서의 이 진균의 중요성을 확인하였다. ring worm의 전형적인 병변은 신체의 노출된 부위에만 발생하였다. M. canis는 환자와 감염된 애완동물의 피부병변에서 분리되었으며 피부 시료로부터 직접 증명되었다. 그림에도 불구하고 비접촉군과 사물성환경으로 부터는 증명되지 않았다. 사람, 개 및 고양이에서 분리된 진균들은 육안적으로나 현미경적으로 서로 비슷한 형태를 나타내었다. 이 진균들의 유전학적 연구결과는 교배(-)형이었다. 동물기호성 표재성진균의 전염은 직접적인 접촉에 의해 자주 일어나는 것으로 사료된다. 이감염증에 대한 역학과 예방방법에 대해 고찰하였다.

• Journal of Welding and Joining
• /
• 제17권3호
• /
• pp.71-78
• /
• 1999

The welded metal bellows is commonly manufactured by welding pairs of washer-shaped discs of thin sheet metal stamped from strip stock in thickness from 0.025 to 0.254 mm. The discs, or diaphragms, are formed with mating circumferential corrugations. In this study, the diaphragms were welded by using a CW Nd: YAG laser to form metal bellows. The bellows was fixed on a jig and compressed axially, while Cu-rings were installed between belows edges for intimate contact of edges. The difference between the inner diameter of bellows and jig shaft causes an eccentricity, while the tolerance between motor shaft and jig shaft causes a wobble type motion. A vision sensor which is based on the optical triangulation was used for seam tracking. An image processing algorithm which can distinguish the image by bellows edge from that by Cu-ring was developed. The geometric relationship which describes the eccentricity and wobble type motion was modeled. The seam tracking using the image processing algorithm and the geometric modeling was performed successfully.

• Genomics & Informatics
• /
• 제4권4호
• /
• pp.147-160
• /
• 2006

GATA transcription factors are widespread eukaryotic regulators whose DNA-binding domain is a class IV zinc finger motif in the form $CX_{2}CX_{17-20}CX_{2}C$followed by a basic region. In fungi, they act as transcriptional activators or repressors in several different processes, ranging from nitrogen source utilization to mating-type switching. Using an in-house bioinformatics portal system, we surveyed 50 fungal and 9 out-group genomes and identified 396 putative fungal GATA transcription factors. The proportion of GATA transcription factors within a genome varied among taxonomic lineages. Subsequent analyses of phylogenetic relationships among the fungal GATA transcription factors, as well as a study of their domain architecture and gene structure, demonstrated high degrees of conservation in type IVa and type IVb zinc finger motifs and the existence of distinctive clusters at least at the level of subphylum. The SFH1 subgroup with a 20-residue loop was newly identified, in addition to six well-defined subgroups in the subphylum Pezizomycotina. Furthermore, a novel GATA motif with a 2f-residue loop ($CX_{2}CX_{21}CX_{2}C$, designated 'zinc finger type IVc') was discovered within the phylum Basidiomycota. Our results suggest that fungal GATA factors might have undergone multiple distinct modes of evolution resulting in diversified cellular modulation in fungi.

• Mycobiology
• /
• 제49권4호
• /
• pp.406-420
• /
• 2021

Gloeostereum incarnatum has edible and medicinal value and was first cultivated and domesticated in China. We sequenced the G. incarnatum monokaryotic strain GiC-126 on an Illumina HiSeq X Ten system and obtained a 34.52-Mb genome assembly sequence that encoded 16,895 predicted genes. We combined the GiC-126 genome with the published genome of G. incarnatum strain CCMJ2665 to construct a genetic linkage map (GiC-126 genome) that had 10 linkage groups (LGs), and the 15 assembly sequences of CCMJ2665 were integrated into 8 LGs. We identified 1912 simple sequence repeat (SSR) loci and detected 700 genes containing 768 SSRs in the genome; 65 and 100 of them were annotated with gene ontology (GO) terms and KEGG pathways, respectively. Carbohydrate-active enzymes (CAZymes) were identified in 20 fungal genomes and annotated; among them, 144 CAZymes were annotated in the GiC-126 genome. The A mating-type locus (MAT-A) of G. incarnatum was located on scaffold885 at 38.9 cM of LG1 and was flanked by two homeodomain (HD1) genes, mip and beta-fg. Fourteen segregation distortion markers were detected in the genetic linkage map, all of which were skewed toward the parent GiC-126. They formed three segregation distortion regions (SDR1-SDR3), and 22 predictive genes were found in scaffold1920 where three segregation distortion markers were located in SDR1. In this study, we corrected and updated the genomic information of G. incarnatum. Our results will provide a theoretical basis for fine gene mapping, functional gene cloning, and genetic breeding the follow-up of G. incarnatum.

• 한국버섯학회지
• /
• 제20권4호
• /
• pp.199-207
• /
• 2022

국내 시장에서는 Wolfiporia hoelen의 균핵 생산량을 늘리기 위해 새로운 균주 육종을 요구되고 있다. 이에 본 연구는 국내에서 수집한 31개의 야생 균주와 12개의 재배 균주에 대해, 최근 보고된 교배형 연관 유전자의 RPB2의 단일염기다형성(single nucleotide polymorphism, SNP)을 적용해 단핵균사와 이핵균사를 구분할 수 있는지 확인함으로써 SNP 정보가 육종에 유용한지 알아 보고자 수행되었다. 이를 위해 해당 정보를 효율적으로 얻을 수 있는 프라이머도 개발하였다. 분석 결과, 국내 야생 균주들은 동형접합인 경우가 중국 균주보다 많아 기존 SNP의 한계를 확인하였다. 이를 보완하고자 RPB2 유전자에서 3개의 SNP를 추가로 발견하였으며, 이를 통해 단핵균사와 이핵균사의 구분 능력을 높였다. 나아가 4개의 SNP를 기존에 육성한 교잡균주와 교잡에 사용한 단포자 균주에 적용함으로써 육종에서의 활용 가능성이 있음을 확인하였다.

• The Plant Pathology Journal
• /
• 제22권3호
• /
• pp.211-214
• /
• 2006

In 2004 and 2005, Phytophthora rot on Luffa cylindrica which had not been reported in Korea occurred in the experimental field at Gyeongsangnam-do Agricultural Research and Extension Services. The disease initiated on leaves and fruits of the plant with small watersoaked dark brown spots and progressed rapidly. The causal pathogen isolated from diseased tissues was identified as a Phytophthora sp. because of aseptate mycelia and zoospores released directly from sporangia. The fungus grew well on PDA and 10% V-8 juice agar showing an arachnoid or rosaceous colony pattern. Sporangia formed abundantly in water and were conspicuously papillate, noncaducous, ovoid to globose, and sized $26\sim62\times19\sim38{\mu}m$. The fungus was heterothallic as producing sexual reproduction structures only when mated with only A2 standard mating type strain. Oogonia and oospores were spherical, smooth walled, and measured as $20\sim28{\mu}m\;and\;16\sim24{\mu}m$, respectively. Oospores were aplerotic and antheridia were amphigynous, unicellula and spherical. Chlamydospores were globose and $20\sim38{\mu}m$ in diameter. Optimum temperature for growth was around $28\sim30^{\circ}C$. The fungus caused similar symptoms on artificially inoculated plant and could be re-isolated thereby proving Koch's postulation. Based on the mycological criteria investigated in this study, the causal fungus of Luffa sylindrica rot was identified as Phytophthora nicotianae. This is the first report of Phytophthora rot of Luffa cylindrica caused by P. nicotianae in Korea.

• 한국임상수의학회지
• /
• 제13권2호
• /
• pp.198-203
• /
• 1996

일반적인 치료에 반응을 나타내지 않는 호흡곤란증에 걸린 4년령의 Shepherd개에서 피부, 눈, 림프절의 병변을 동반한 치명적인 파종성 크립토콕스병이 진단되었다. 세포학적검사에 의해 난원형 내지 구형이고, 두꺼운 협막을 갖고 있는, 형태학적으로 Cryptococcus neoformans와 일치하는 효모균이 증명되었다. 이 병원체는 혈액, 오줌, 콧물, 견갑전림프절 흡인물, 피부생검 시료, 피부 면봉 시료 등을 25$\circ $C의 Pal씨 배지에 접종하여 용이하게 분리되었다 배양물을 PHOL염색액으로 염색하여 현미경으로 검사한 결과 얇은 막에 싸인 발아를 나타내거나. 나타내지 않는 구형 내지 난원형의 효모균이 증명되었다. 이 개는 ketaconazole 로 치료를 시작 한 후 6일만에 폐사하였다. 공기, 흙, 비둘기 배설물, 톱밥 등을 Pal씨 배지에 접종하여 C.neoformans가 배양됨으로써 역학적으로 환경이 병원소 역할을 한 것으로 판단되었다. 환축과 환경으로부터 분리된 균주는 세밀한 동정결과 neoformans (serotype AD)에 속하며, Filobasidiella neoformans "alpha" mating type을 나타내었다. 이 연구결과, Pal씨 배지가 크립토콕스병의 조기진단과 역학적 조사에 훌륭한 감별배지라는 것이 입증되었다. 개량된 Pal씨 배지는 C. neoformans의 genetic crossing을 판단하는데 성공적으로 사용할 수 있었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제26권11호
• /
• pp.1536-1544
• /
• 2013

A least squares regression interval mapping model was derived to detect quantitative trait loci (QTL) with a unique mode of genomic imprinting, polar overdominance (POD), under a breed cross design model in outbred mammals. Tests to differentiate POD QTL from Mendelian, paternal or maternal expression QTL were also developed. To evaluate the power of the POD models and to determine the ability to differentiate POD from non-POD QTL, phenotypic data, marker data and a biallelic QTL were simulated on 512 F2 offspring. When tests for Mendelian versus parent-of-origin expression were performed, most POD QTL were classified as partially imprinted QTL. The application of the series of POD tests showed that more than 90% and 80% of medium and small POD QTL were declared as POD type. However, when breed-origin alleles were segregating in the grand parental breeds, the proportion of declared POD QTL decreased, which was more pronounced in a mating design with a small number of parents ($F_0$ and $F_1$). Non-POD QTL, i.e. with Mendelian or parent-of-origin expression (complete imprinting) inheritance, were well classified (>90%) as non-POD QTL, except for QTL with small effects and paternal or maternal expression in the design with a small number of parents, for which spurious POD QTL were declared.