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DETERMINATION OF MOISTURE AND NITROGEN ON UNDRIED FORAGES BY NEAR INFRARED REFLECTANCE SPECTROSCOPY(NIRS)

  • Cozzolino, D.;Labandera, M.;Inia La Estanzuela
    • Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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    • 2001.06a
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    • pp.1620-1620
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    • 2001
  • Forages, both grazed and conserved, provide the basis of ruminant production systems throughout the world. More than 90 per cent of the feed energy consumed by herbivorous animals world - wide were provided by forages. With such world - wide dependence on forages, the economic and nutritional necessity of been able to characterize them in a meaningful way is vital. The characterization of forages for productive animals is becoming important for several reasons. Relative to conventional laboratory procedures, Near Infrared Reflectance Spectroscopy (NIRS) offers advantages of simplicity, speed, reduced chemical waste, and more cost-effective prediction of product functionality. NIR spectroscopy represents a radical departure from conventional analytical methods, in that entire sample of forage is characterized in terms of its absorption properties in the near infrared region, rather than separate subsamples being treated with various chemicals to isolate specific components. This forces the analyst to abandon his/her traditional narrow focus on the sample (one analyte at a time) and to take a broader view of the relationship between components within the sample and between the sample and the population from which it comes. forage is usually analysed by NIRS in dry and ground presentation. Initial success of NIRS analysis of coarse forages suggest a need to better understand the potential for analysis of minimally processed samples. Preparation costs and possible compositional alterations could be reduced by samples presented to the instrument in undried and unground conditions. NIRS has gained widespread acceptance for the analysis of forage quality constituents on dry material, however little attention has been given to the use of NIRS for chemical determinations on undried and unground forages. Relatively few works reported the use of NIRS to determine quality parameters on undried materials, most of them on both grass and corn silage. Only two works have been found on the determination of quality parameters on fresh forages. The objectives of this paper were (1) to evaluate the use of NIRS for determination of nitrogen and moisture on undried and unground forage samples and (2) to explore two mathematical treatments and two NIR regions to predict chemical parameters on fresh forage. Four hundred forage samples (n: 400) were analysed in a NIRS 6500 instrument (NIR Systems, PA, USA) in reflectance mode. Two mathematical treatments were applied: 1,4,4,1 and 2,5,5,2. Predictive equations were developed using modified partial least squares (MPLS) with internal cross - validation. Coefficient of determination in calibration (${R^2}_{CAL}$) and standard error in cross-validation (SECV) for moisture were 0.92 (12.4) and 0.92 (12.4) for 1,4,4,1 and 2,5,5,2 respectively, on g $kg^{-1}$ dry weight. For crude protein NIRS calibration statistics yield a (${R^2}_{CAL}$) and (SECV) of 0.85 (19.8) and 0.85 (19.6) for 1,4,4,1 and 2,5,5,2 respectively, on a dry weight. It was concluded that NIRS is a suitable method to predict moisture and nitrogen on fresh forage without samples preparation.

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Thallium-201 Scan in Bone and Softtissue Sarcoma - Comparison with Tc-99m-MIBI and Tc-99m-MDP Scan - (악성 골 및 연부조직 종양에서 Tl-201 SCAN의 진단적 효능 - Tc-99m-MIBI 및 Tc-99m-MDP scan과의 비교 -)

  • Shin, Duk-Seop;Cho, Ihn-Ho;Ahn, Jong-Chul;Ahn, Myun-Hwan;Lee, Sang-Ho
    • The Journal of the Korean bone and joint tumor society
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    • v.2 no.1
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    • pp.1-7
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    • 1996
  • PURPOSE : The purpose of this study is to know the ability of detecting malignant tumor tissue by Tl-201 scan, and to compare with that of Tc-99m-MIBI and Tc-99m-MDP scan. MATERIAL AND METHODS : Between February 1994 and December 1995,38 unselected patients with various bone pathologies were studied prospectively. Eighteen had malignant bone and soft tissue pathologies, while twenty had benign. All patients were studied with Tl-201, Tc-99mMIBI and Tc-99m-MDP scan prior to surgical biopsy. PICKER Prism 2000 gamma camera with high resolution parallel hole collimator was used for scanning. To avoid the interaction of isotope, the early(30min.) and delayed phase(3hrs.) of Tl-20l scan was performed first and Tc-99m-MIBI scan was performed after 30 minutes, and then Tc-99m-MDP scan 48 hours later. The scan images were visually evaluated by a blinded nuclear medicine physician. We could find true positive, true negative, false positive and false negative by the comparison of results with those of biopsy. We calculated positive and negative predictive value(%), sensitivity(%), specificity(%) and diagnostic accuracy(%) of each scan. RESULT : The results of each scan were 85.7, 100, 100, 85, 92.1% in Tl-201, 81, 94.1, 94.4, 80, 86.8% in Tc-99m-MIBI and 50, 66.7, 88.9, 20, 52.6% in Tc-99m-MDP scan. As a conclusion, Tl-201 scan was the most specific and accurate method for detecting malignant tumor tissue. Tc-99m-MIBI scan was also good for malignant tumor searching. CONCLUSION : With our results, we can use Tl-201 scan to differentiate benign from malignant tumor, and to evaluate the response of preoperative chemotherapy or radiotherapy, and to determine the residual tumor or local recurrence. For the better result, we need to have a more detail information about false positive cases and a more objective and quantitative reading technique.

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The Effect of the Compound of Tomato Extract to the Prostatic Cancer Cell and the Prostate of the Rat Model of Benign Prostatic Hyperplasia (토마토 추출액 복합체가 전립선 암 세포와 전립선 비대증에 미치는 영향)

  • Kang, Han-Saem;Kim, Gwang-Yun;Jung, Il;Oh, Sung-Dug;Kim, Chang-Hoon;Shim, Bong-Sup;Park, Keun-Hyung;Oh, Suk-Jung
    • Korean Journal of Pharmacognosy
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    • v.38 no.2 s.149
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    • pp.197-203
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    • 2007
  • Benign prostatic hyperplasia (BPH) is one of the common disease in elderly men. Recently old-age population is increased and we are growing more and more interested in clinical importance of BPH. In this study, the effect of PLX, which was the mixture of tomato extract (including 2% of lycopene) and chitooligosaccharide, on prostatic cancer cell and testosterone-induced BPH in adult rats of the Sprague Dawley strain was determined. The cell viability was evaluated by MTT method using L929 and LNCaP cell line, pretreated with various concentrations of PLX. The expression of prostatic specific antigen (PSA) and 5${\alpha$}$-reductase genes were evaluated by realtime PCR using LNCaP cell line and compared various concentrations of PLX with 50 ${\mu}$M of finasteride. An experimental prostatic hyperplasia was induced in male Sprague Dawley rats by giving testosterone for 8 weeks. After 2 weeks from start of giving testosterone, PLX and finasteride were administered orally once a day. The results were analyzed with prostate weight per body weight at 8 weeks. Cell viability of L929 cell line decreased specifically at the concentration of 2000 ${\mu}$g/mf of PLX. The cytotoxicity of PLX to the LNCaP cell line was shown at above 500 ${\mu}$g/ml of PLX. The inhibitory effect of PLX to the expression of PSA and 5${\alpha$}$-reductase genes in LNCaP cell line increased with the concentration of PLX. In vivo study, the results of PLX and finasteride administered group were 3.75${\pm}$0.60 and 3.49${\pm}$0.49 prostate weight ${\times}10^3$/body weight, which were lower than the result of BPH induced group (4.74${\pm}$0.58). These results suggested that PLX may be an effective material in BPH by having the role of the 5a-reductase inhibitor.

Variation of the Overall Heat Transfer Coefficient of Plastic Greenhouse Covering Material (플라스틱온실 피복재의 관류열전달계수 변화)

  • Lee, Hyun-Woo;Diop, Souleymane;Kim, Young-Shik
    • Journal of Bio-Environment Control
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    • v.20 no.2
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    • pp.72-77
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    • 2011
  • The objective of the present study is to provide the basic data necessary for estimating the overall heat transfer coefficient of commercial plastic greenhouse. The heat flow through covering of greenhouses was measured and the variation of overall heat transfer coefficient was analyzed. Because the inside-outside temperature difference of greenhouse to indicate the stabilized overall heat transfer coefficient was different depending on the number of covering layers, the actual overall heat transfer coefficient should be decided in range of inside-outside temperature difference to make the coefficient constant for each covering method. The variation trend of the overall heat transfer coefficient according to the inside-outside temperature difference corresponded with the existing research results, but the specific values of temperature difference to present the stabilized overall heat transfer coefficient were different each other. The increase rates of overall heat transfer coefficient with wind speed were quite dissimilar among several research results and the quantity of heat loss through covering according to the wind speed in the double layers covered or curtained greenhouse was less than that in the single layer covered greenhouse. Because there was large variations among the values of overall heat transfer coefficient for the polyethylene film greenhouses, it was required to establish the standardized environmental condition for experiment measuring heat flow through covering in commercial greenhouse.

Change of Diaphragmatic Level and Movement Following Division of Phrenic Nerve (횡격막 신경 차단 후 횡격막 위치 및 운동의 변화)

  • 최종범;김상수;양현웅;이삼윤;최순호
    • Journal of Chest Surgery
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    • v.35 no.10
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    • pp.730-735
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    • 2002
  • Diaphragm is innervated by phrenic nerve and lower intercostal nerves. For patients with avulsion injury of brachial plexus, an in situ graft of phrenic nerve is frequently used to neurotize a branch of the brachial plexus. We studied short-term and mid-term changes of diaphragmatic level and movement in patients with dissection of phrenic nerve for neurotization. Material and Method : Thirteen patients with division of either-side phrenic nerve for neurotization of musculocutaneous nerve were included in this study. With endoscopic surgical procedure, the intrathoracic phrenic nerve was entirely dissected and divided just above the diaphragm. The dissected phrenic nerve was taken out through thoracic inlet and neck wound and then anastomosed to the musculocutaneous nerve through a subcutaneous tunnel. With chest films and fluoroscopy, levels and movements of diaphragm were measured before and after operation. Result : There was no specific technical difficulty or even minor postoperative complications following endoscopic division of phrenic nerve. After division of phrenic nerve, diaphragm was soon elevated about 1.7 intercostal spaces compared with the preoperative level, but it did not show paradoxical motion in fluoroscopy. More than 1.5 months later, diaphragm returned downward close to the preoperative level (average level difference was 0.9 intercostal spaces; p=NS). Movement of diaphragm was not significantly decreased compared with the preoperative one. Conclusion : After division of phrenic nerve, the affected diaphragm did not show a significant decrease in movement, and the elevated diaphragm returned downward with time. However, the decreased lung volumes in the last spirometry suggest the decreased inspiratory force following partial paralysis of diaphragm.

A Study on the Sediment Transport using Radioisotope Tracer (방사성동위원소 추적자를 이용한 표사이동 추적실험)

  • Choi Byung-Jong;Jung Sung-Hee;Kim Jong-Bum;Lee Jong-Sup
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.16 no.3
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    • pp.162-170
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    • 2004
  • On the basis of the radiotracer technology and the related equipments which have been developed for its industrial application through the nuclear long-term research project, a radiotracer study on sediment transport was carried out as a part of the development of the radiotracer technology for a coastal environment. The crystalline material doped with iridium having a similar composition and specific gravity as those of the bedload sand collected from the research area was produced by the oxide-route method. A radioisotope container was specially designed to inject the radiotracer from 1 m above the sea bedload without radioactive contamination during the transport from the nuclear reactor at KAERI. The position data from the DGPS and the radiation measurement data were collected concurrently and stored by means of the application software programmed with the LabVIEW of the National Instrument. The position data was reprocessed to represent the real position of the radiation probe under water and not that of the DGPS antenna on board. The time dependency of the spatial distribution of the sediment was studied in the area through three tracking measurements after the iridium glass was injected. This trial application showed the potential of the radiotracer technology as an important role for maintaining and developing the coastal environment in the future.

Analysis of children's Reaction in Facial Expression of Emotion (얼굴표정에서 나타나는 감정표현에 대한 어린이의 반응분석)

  • Yoo, Dong-Kwan
    • The Journal of the Korea Contents Association
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    • v.13 no.12
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    • pp.70-80
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    • 2013
  • The purpose of this study has placed its meaning in the use as the basic material for the research of the person's facial expressions, by researching and analyzing the visual reactions of recognition of children according to the facial expressions of emotion and by surveying the verbal reactions of boys and girls according to the individual expressions of emotion. The subjects of this study were 108 children at the age of 6 - 8 (55 males, 53 females) who were able to understand the presented research tool, and the response survey conducted twice were used in the method of data collection by individual interviews and self administered questionnaires. The research tool using in the questionnaires were classified into 6 types of joy, sadness, anger, surprise, disgust, and fear which could derive the specific and accurate responses. Regarding children's visual reactions of recognition, both of boys and girls showed the high frequency in the facial expressions of joy, sadness, anger, surprise, and the low frequency in fear, disgust. Regarding verbal reactions, it showed the high frequency in the heuristic responses either to explore or the responds to the impressive parts reminiscent to the facial appearances in all the joy, sadness, anger, surprise, disgust, fear. And it came out that the imaginary responses created new stories reminiscent to the facial expression in surprise, disgust, and fear.

Adsorption Characteristics of Sulfonamide Antibiotic Compounds in GAC Process (GAC 공정에서의 Sulfonamide계 항생물질 흡착특성)

  • Son, Hee-Jong;Jung, Jong-Moon;Roh, Jae-Soon;Yu, Pyung-Jong
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.4
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    • pp.401-408
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    • 2008
  • Adsorption performance of sulfonamide antibiotic compounds such as sulfadimethoxine(SDM), sulfachloropyridazine(SCP), sulfamethazine(SMT), sulfathiazole(STZ) and sulfamethoxazole(SMX) on granular activated carbon(GAC) was evaluated in this study. The coal-based activated carbon was found to be more effective than other carbons in adsorption of sulfonamide antibiotic compounds. The wood-based activated carbon was less effective than coconut- and coal-based carbon in adsorption nevertheless having larger pore volume and specific surface area than others carbons. The maximum adsorption capacities(X/M) of coal-based activated carbon for the five sulfonamide species was 1.3$\sim$1.5 and 1.8$\sim$2.1 times larger than coconut- and wood-based activated carbon, respectively. Carbon usage rates (CUR) of coal-, coconut- and wood-based activated carbons for SCP were 3.55 g/day, 4.29 g/day and 6.47 g/day, respectively. Similar results were obtained in the adsorption of the rest four sulfonamide species. It is concluded that coal-based activated carbon could removed the sulfonamide antibiotic compounds better than other material-based activated carbons.

The Influence of Oxygen Gas Flow Rate on Growth of Tin Dioxide Nanostructures (이산화주석 나노구조물의 성장에서 산소가스 유량이 미치는 영향)

  • Kim, Jong-Il;Kim, Ki-Chul
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.10
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    • pp.1-7
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    • 2018
  • Tin dioxide, $SnO_2$, is applied as an anode material in Li-ion batteries and a gas sensing materials, which shows changes in resistance in the presence of gas molecules, such as $H_2$, NO, $NO_2$ etc. Considerable research has been done on the synthesis of $SnO_2$ nanostructures. Nanomaterials exhibit a high surface to volume ratio, which means it has an advantage in sensing gas molecules and improving the specific capacity of Li-ion batteries. In this study, $SnO_2$ nanostructures were grown on a Si substrate using a thermal CVD process with the vapor transport method. The carrier gas was mixed with high purity Ar gas and oxygen gas. The crystalline phase of the as-grown tin oxide nanostructures was affected by the oxygen gas flow rate. The crystallographic property of the as-grown tin oxide nanostructures were investigated by Raman spectroscopy and XRD. The morphology of the as-grown tin oxide nanostructures was confirmed by scanning electron microscopy. As a result, the $SnO_2$ nanostructures were grown directly on Si wafers with moderate thickness and a nanodot surface morphology for a carrier gas mixture ratio of Ar gas 1000 SCCM : $O_2$ gas 10 SCCM.

Effects of Activated Carbon Types and Service Life on Adsorption of Tetracycline Antibiotic Compounds in GAC Process (활성탄 재질 및 사용연수에 따른 Tetracycline계 항생물질 흡착특성)

  • Son, Hee-Jong;Jung, Jong-Moon;Hwang, Young-Do;Roh, Jae-Soon;Yu, Pyung-Jong
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.9
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    • pp.925-932
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    • 2008
  • Adsorption performance of tetracycline antibiotic compounds such as tetracycline(TC), oxytetracycline(OTC), chlortetracycline (CTC) and minocycline(MNC) on granular activated carbon(GAC) was evaluated in this study. The coal-based activated carbon was found to be more effective than other carbons in adsorption of tetracycline antibiotic compounds. The wood-based activated carbon was less effective than coconut- and coal-based carbon in adsorption nevertheless having larger pore volume and specific surface area than others carbons. The maximum adsorption capacities(X/M) of coal-based activated carbon for the four tetracycline species was 1.27$\sim$1.36 and 1.69$\sim$1.84 times larger than coconut- and wood-based activated carbon, respectively. Carbon usage rates(CUR) of coal-, coconut- and wood-based activated carbons for tetracycline(TC) were 2.96 g/day, 3.40 g/day and 4.53 g/day, respectively. Similar results were obtained in the adsorption of the rest three tetracycline species. It is concluded that coal-based activated carbon could removed the tetracycline antibiotic compounds better than other material-based activated carbons.