• Journal of Microbiology and Biotechnology
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• v.27 no.7
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• pp.1223-1232
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• 2017

Cordyceps militaris, a member of Ascomycota, a mushroom referred to as caterpillar Dong-chung-ha-cho, is commercially valuable because of its high content of bioactive substances, including cordycepin, and its potential for artificial cultivation. Cordycepin (3'-deoxyadenosine) is highly associated with the pharmacological effects of C. militaris. C. militaris is heterothallic in that two mating-type loci, idiomorph MAT1-1 and MAT1-2, exist discretely in two different spores. In this study, nine C. militaris strains were mated with each other to prepare newly bred strains that produced a larger amount of cordycepin than the parent strains. Nine strains of C. militaris were identified by comparing the internal transcribed spacer sequence, and a total of 12 single spores were isolated from the nine strains of C. militaris. After the MAT idiomorph was confirmed by PCR, 36 mating combinations were performed with six single spores with MAT1-1 and the others with MAT1-2. Eight mating combinations were successfully mated, producing stroma with perithecia. Cordycepin content analysis of all strains by high-performance liquid chromatography revealed that the KASP4-bred strain produced the maximum cordycepin among all strains, regardless of the medium and stroma parts. Finally, universal rice primer-PCR was performed to demonstrate that the bred strains were genetically different from the parental strains and new C. militaris strains. These results may be related to the recombination of genes during mating. The newly produced strains can be used to meet the industrial demand for cordycepin. In addition, breeding through mating suggests the possibility of producing numerous cordycepin-producing C. militaris strains.

• Geotechnical Engineering
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• v.11 no.2
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• pp.51-70
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• 1995

Types of foundation of high rise buildings are primarily determined by loads transmitted from super structure, soil bearing capacity and available construction technology, The use of deep foundation of the buildings considered in this study due to the fact that rock of enough bearing capacity is not found down until 90~l00m. When a concentration of high soil pressure must be distributed over the entire building area, when small soft soil areas must be bridged, and when compressible strata are located at a shallow depth, mat foundation may be useful in order to have settlement and differential settlement of variable soils be minimized. The concept of mat foundation will also demonstrate some difficulties of applications if the load bearing demand directly carried down to the load -bearing strata exceeds the load -bearing capacity. This paper introduces both the analysis and design of mat type foundation for high rise buildings as well as the method-ology of modelling of the soil foundation, especially, engineered to redistribute the stress exceeding the soil bearing capacity. This process will result in the wide spread of stresses over the entire building foundation.

• Corrosion Science and Technology
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• v.7 no.6
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• pp.338-343
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• 2008

The electrochemical properties of W-modified austenitic stainless steels containing Ta and La were evaluated in a $H_{3}PO_{4}$ type PEMFC environment. Electrochemical test was conducted in 0.05 M $H_{3}PO_{4}$ solution at $80^{\circ}C$ and electrical property was conducted by contact resistance test. XPS was conducted to analyze the chemical elements consisting of passive film. Addition of La and Ta in W-modified austenitic stainless steel shows not only better corrosion resistance but also better electrical property.

• Journal of Korean Society on Water Environment
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• v.30 no.6
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• pp.638-646
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• 2014

Soil erosion from agricultural fields leads to various environmental problems weakening the capabilities of flood control and ecosystem in water bodies. Regarding these problems, Ministry of Environment of South-Korea prepared various structural and non-structural best management practices (BMPs) to control soil erosion. However, a lot of efforts are required to monitor and develop BMPs. Thus, modeling techniques have been developed and utilized for these issues. This study estimated the effectiveness of BMPs which are a vegetation mat with infiltration roll and Roll type vegetation channel using Soil and Water Assessment Tool (SWAT) model through the adjustment of the conservation practice factors, P factors, for Universal Soil Loss Equation which were calculated by monitoring data collected at the segment plots. Each BMP was applied to the areas with slopes ranged from 7% to 13% in the Haeanmyeon watershed. As a result of simulation, the vegetation mat with infiltration roll and Roll type vegetation channel showed 55% and 59% efficiency of soil erosion reduction, respectively. Also, Vegetation mat with infiltration roll and Roll type vegetation channel showed each 11.2% and 11.8% efficiency in reduction of sediment discharge. These roll type vegetation channel showed greater efficiency of soil erosion reduction and sediment discharge. Based on these results, if roll type vegetation channel is widely used in agricultural fields, reduction of soil erosion and sediment discharge of greater efficiency would be expected.

• The Journal of the Korea Contents Association
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• v.12 no.8
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• pp.366-372
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• 2012

In most of construction work, especially mat foundation construction, it is very important that accidents be prevented at early aged construction stage. To reduce the risk of accidents, it is necessary to choose the curing method to provide instructions for safety forming work by proper curing of concrete after placing. Therefore, this paper has proposed a designation algorithm of curing method for representing curing type and period by analyzing the transient hydration heat solution, focusing on mat foundation construction. This has been implemented, moreover, using an object oriented programming language to develop structural analysis system taking account crack index. In addition, we present in this paper a safety curing management system developed to assess risk during forming work.

• Journal of the Korean Geosynthetics Society
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• v.4 no.1
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• pp.27-36
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• 2005

Discharge capacity of the fiber mat and the fiber drain made with natural fibers abstracted from plant source was evaluated by permeability test for fiber mat and Delft type test and composite discharge capacity test using disturbed clayey soils for fiber drain. The permeability test results for environmentally friendly coconut fiber mat prove that fiber mat has outstanding permeability in substituting permeable sand. However, discharge capacity of fiber drain evaluated by conventional Delft type discharge capacity test was relatively lower than that of plastic drain board. Nevertheless, settlement and pore pressure dissipation behaviors of fiber drain and plastic drain board installed clay soil during the composite discharge capacity test were almost similar to that of plastic drain board. It is found that the natural fiber drain satisfies requiring minimum discharge capacity in substituting the conventional plastic drain board.

• The Korea Journal of Herbology
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• v.29 no.1
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• pp.27-33
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• 2014

Objectives : This study was carried out to identify DNA markers of "Allium sativum" be circulated from Korea and China, which is difficult to discriminate from morphological characters because of fragmental materials of bulb. That is, all these studies focused on the discrimination of Allium sativum L. But these day, Chinese A. sativum was in circulated Korean A. sativum in Korean medicine markets. Therefore, the purpose of our study was to develop molecular markers for discrimination between Korean A. sativum and imports from China. Methods : Materials were collected randomly from a markets in Korea and China and be analyzed with matK, ndhF and trnL-F regions of chloroplast DNA (cpDNA). We collected 45 A. sativum individuals from Korean and Chinese medicine markets, in 2013. Results : As a results, matK and ndhF regions of cpDNA was shown to be identify, Species that grow from warm place and cold place can divide as five SNP (Single nucleotide polymorphisms) markers in matK and ndhF genes. Also, in trnL-F regions, found one SNP that can divide Korean A. sativum and Chinese A. sativum. Conclusions : From the analysis of matK and ndhF regions of cpDNA, we presumed that three markers of cpDNA were found by useful marker that can distinguish Korean, Chinese, Warm place type, and Cold place type. Individual differences of Korean and Chinese was thought that appear in geographical difference and genetic difference by environment for long hour even if same species.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2003.09a
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• pp.106-109
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• 2003

Many diverse methods have been developing in the field of biometric identification as human-friendliness has been emphasized in the intelligent system's area. One of emerging method is to use human footprint. Automated footprint-based person recognition was started by Nakajima et al.'s research but they showed relatively low recognition result by low spatial resolution of pressure sensor and standing posture. In this paper, we proposed a modified Nakajima's method to use walking footprint which could give more stable toe information than standing posture. Finally, we prove the usefulness of proposed method as 91.4tt recognition rate in 11 volunteers' test.

• Journal of Biosystems Engineering
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• v.14 no.1
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• pp.8-15
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• 1989

Agricultural materials do not react in a purely elastic manner, and their responses when subjected to stress and strain are appeared from a combination of elastic and viscous behavior. Various researchers have studied the mechanical and rheological properties of the many agricultural materials, but those properties are available mostly foreign varieties of agricultural products. Rheological properties of rice seedlings become important to formulate the principles governing their mechanical behavior. The objectives of this study were to experimentally determine the stress relaxation properties of rice seedlings such as three Japonica-type and one Indica ${\times}$ Japonica hybrid in the transplanting age. The results of this study are summarized as follows; 1. The stress relaxation behavior could be described by the generalized Maxwell model. 2. The phenomenon of stress relaxation happened abruptly just after loading and this phenomenon weakened with the loading time lapsed. 3. With increase of the initial stress, the stress relaxation intensity and residual stress increased, while the relaxation time was constant with increased, while the relaxation time was constant with increase of the level of initial stress. 4. With increase of loading rate, the stress relaxation intensity increased, while the relaxation time and residual stress decreased.

• Proceedings of the KSR Conference
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• 2003.10b
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• pp.552-556
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• 2003

Expansion joint is the absorbing structure when the upper structure of bridge have the drying shrinkage and creep from the change of temperature or construction badness. Because it has the fatigue from continuous vehicles traffic. the durability and performance is verified by the static and dynamic loading test In this study. we make the decision that T-MAT type expansion joint is applied in railway bridge and the expansion joint was tested by the criterion of Korea High Speed Rail Construction Authority.