The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

The Study of DNA markers to identify of Allium sativum L.

한약재 마늘(Allium sativum L.)의 식별을 위한 유전자 감식연구

  • Son, OGyeong (Department of Biology, College of Science, Yeungnam University) ;
  • Seo, Bu-II (Department of Herbology, College of Oriental medicine, Daegu Haany University) ;
  • Lee, Seon-Ha (College of Health, Kyungwoon University) ;
  • Park, Seon-Joo (Department of Biology, College of Science, Yeungnam University)
  • Received : 2013.12.13
  • Accepted : 2014.01.20
  • Published : 2014.01.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study was carried out to identify DNA markers of "Allium sativum" be circulated from Korea and China, which is difficult to discriminate from morphological characters because of fragmental materials of bulb. That is, all these studies focused on the discrimination of Allium sativum L. But these day, Chinese A. sativum was in circulated Korean A. sativum in Korean medicine markets. Therefore, the purpose of our study was to develop molecular markers for discrimination between Korean A. sativum and imports from China. Methods : Materials were collected randomly from a markets in Korea and China and be analyzed with matK, ndhF and trnL-F regions of chloroplast DNA (cpDNA). We collected 45 A. sativum individuals from Korean and Chinese medicine markets, in 2013. Results : As a results, matK and ndhF regions of cpDNA was shown to be identify, Species that grow from warm place and cold place can divide as five SNP (Single nucleotide polymorphisms) markers in matK and ndhF genes. Also, in trnL-F regions, found one SNP that can divide Korean A. sativum and Chinese A. sativum. Conclusions : From the analysis of matK and ndhF regions of cpDNA, we presumed that three markers of cpDNA were found by useful marker that can distinguish Korean, Chinese, Warm place type, and Cold place type. Individual differences of Korean and Chinese was thought that appear in geographical difference and genetic difference by environment for long hour even if same species.

Keywords

References

  1. Lee TB. Illustrated Flora of Korea. Seoul : Hyangmoonsa. 1979 : 701.
  2. The textbook compilation committee of Korean pharmacognosy. Herbal medicine. Seoul : Academy Book. 2006 : 882-5.
  3. Kim SK, Kyung KH, Ko BK, Kim SS, Park JH. Dictionary of food science and technology. Paju : Kyomunsa. 2012 : 196.
  4. Kwon SK. Organosulfur Compounds from Allium sativum and Physiological Activities. Biomol Ther. 2003 ; 11(1) : 8-32.
  5. Kim KJ, Do JR, Kim HK. Antimicrobial, Antihypertensive and Anticancer Activities of Garlic Extracts. Korean J Food Sci Technol. 2005 ; 37(2) : 228-323.
  6. Cha BC, Lee EH. Inhibition activity of Garlic on a drug metabolizing enzyme CYP3A4. Kor J Pharmacogn. 2006 ; 37(2) : 97-102.
  7. Jeong CH, Bae YI, Lee JH, Roh JG, Shin CS, Choi JS, Shim KH. Chemical Components and Antimicrobial Activity of Garlics from Different Cultivated Area. J Agric Life Sci. 2009 ; 43(1) : 51-9.
  8. Shin JH, Lee SJ, Jung WJ, Kang MJ, Sung NJ. Physicochemical characteristics of Garlic (Allium sativum L.) on collected form the different regions. J Agric Life Sci. 2011 ; 45(2) : 103-14.
  9. Jeong JY, Woo KS, Hwang IG, Yoon HS, Lee YR, Jeong HS. Effects of heat treatment and antioxidant activity of aroma on garlic harvested in different cultivation areas. J Korean Soc Food Sci Nutr. 2007 ; 36(12) : 1637-42. https://doi.org/10.3746/jkfn.2007.36.12.1637
  10. Lee YK, Yoon YH, Chung IS, Lee JS, Lim SJ, Song IG, Kim DU. Analysis of Genetic Relationship by RAPD Technique in Allium sativum L. Korean J Breed. Sci. 1996 ; 28(3) : 332-41.
  11. Choi HY, Kim DW, Kim DE, Suh YB, Ham IH. The Relative Identification of C. officinale and L. chuanxiong by PCR-Mediated Fingerprinting. Kor J Herbology 2005 ; 20(4) : 151-61.
  12. Kim MK, Jigden B, Sun H, Yang DC. Phylogenetic analysis of Ji-Mo (Anemarrhena asphodeloides) on the basis of trnL-F sequences. Korean J Medicinal Crop Sci. 2008 ; 16(1) : 20-6.
  13. Kim JH., Kim dohg Kap Kim, Foest F, Fay MF, Chase MW. Molecular phylogenetics of Ruscaceae sensu lato and related families (Asparagales) based on plastid and nuclear DNA sequens. Ann Bot. 2010 ; 106 : 775-90. https://doi.org/10.1093/aob/mcq167
  14. Doyle J, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 1987 ; 19 : 11-5.
  15. YU J, XUE JH, ZHOU SL. New universal matK primers for DNA barcoding angiosperms. J Syst Evol. 2011 ; 49(3) : 176-81. https://doi.org/10.1111/j.1759-6831.2011.00134.x
  16. Clark LG, Zhang W, Wendel JF. A phylogeny of the grass family (Poaceae) based on ndhF sequence data. Syst Bot. 1995 ; 20(4) : 436-60. https://doi.org/10.2307/2419803
  17. Taberlet P, Gielly L, Pautou G, Bouvet J. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol. 1991 ; 17 : 1105-09. https://doi.org/10.1007/BF00037152
  18. Drummond AJ, Ashton, Buxton S, Cheung M, Cooper A, Duran C, Field M, Heled J, Kearse M, Markowitz S, Moir R, Stones-Havas S, Sturrock S, Thierer T, Wilson A. Geneious v6.1.6, Available from http://www.geneious.com/. 2013.
  19. Swofford DL. PAUP* : Phylogenetic analysis using parsimony, ver. 4.10b. Sinauer Associates, Sunderland, MA. USA. 2002.
  20. Felsenstein J. Confidence limits on phylogenies : an approach using the bootstrap. Evolution. 1985 ; 39 : 783-91. https://doi.org/10.2307/2408678
  21. Farris JS, Albert VA, Kallersjo M, Lipscomb D, Kluge AG. Parsimony jackknifing outperforms neighborjoining. Cladistics 1996 ; 12 : 99-124. https://doi.org/10.1111/j.1096-0031.1996.tb00196.x
  22. Kimura M. A simple method for estimating evolutionary rates of base substitution through comparative studies of nucleotide sequences. J Mol Evol. 1980 ; 16 : 111-20. https://doi.org/10.1007/BF01731581
  23. Saitou N, Nei M. The neighbor-joining method : A new for reconstructing phylogenetic trees. Mol Biol Evol. 1987 ; 4 : 406-25.
  24. Hwang WI, Lee SD, Son HS, Baik NG, Ji RH. Effect of Fresh Garlic Extract on the tumor cell growth and immunopotentiating activity. J Korean Soc Food Nutr. 1990 ; 19(5) : 494-508.
  25. Knowles LM, Milner JA. Allyl sulfides modify cell growth. Drug Metabol Drug Interact. 2000 ; 17 : 81-107.
  26. Sheo HJ, Seo YS. A Study on the Antidotal Effects of Dietary Garlic Juice on Lead Poisoning Rats. J Korean Soc Food Sci Nutr. 2005 ; 34(3) : 342-50. https://doi.org/10.3746/jkfn.2005.34.3.342