• Journal of Soil and Groundwater Environment
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• v.14 no.6
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• pp.1-18
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• 2009

A series of three-dimensional numerical simulations using a generalized multidimensional hydrodynamic dispersion numerical model is performed to simulate effectively and to evaluate quantitatively impacts of urbanization on density-dependent groundwater flow and salt transport in a coastal aquifer system, Suyeong-Gu, Busan, Korea. A series of steady-state numerical simulations of groundwater flow and salt transport before urbanization with material properties of geologic formations, which are established by numerical modeling calibrations considering all the urbanization factors, is performed first without considering all the urbanization factors. A series of transient-state numerical simulations of groundwater flow and salt transport after urbanization is then performed considering the urbanization factors individually and all together. Finally, the results of both numerical simulations are compared with each other and analyzed. The results of the numerical simulations show that density-dependent groundwater flow, salt transport, and seawater intrusion in the coastal aquifer system are intensively and extensively impacted by the urbanization factors. Especially, these urbanization factors result in the changes of the total groundwater volume and salt mass in the coastal aquifer system. However, such impacts of each urbanization factor are not spatially uniform but locally different.

• Journal of the Korea Concrete Institute
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• v.15 no.2
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• pp.314-322
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• 2003

Pipe cooling method is widely used for reduction of hydration heat and control of cracking in mass concrete structures. However, in order to effectively apply pipe cooling systems to concrete structures, the coefficient of flow convection relating the thermal transfer between inner stream of pipe and concrete must be estimated. In this study, a device measuring the coefficient of flow convection was developed. Since a variation of thermal distribution caused by pipe cooling has a direct effect on internal forced flows, the developed testing device is based on the internal forced flow concept. Influencing factors on the coefficient of flow convection are mainly flow velocity, pipe diameter and thickness, and pipe material. Using experimental results from the developed device, the coefficient of flow convection was calculated. Finally, a general prediction model was proposed by theoretical procedures. The proposed prediction model is able to estimate the coefficient of flow convection with flow velocity and material properties of pipe. From comparison with experimental results, the coefficient of flow convection by this model was well agreed with those by experimental results.

• Journal of the Korea Concrete Institute
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• v.20 no.6
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• pp.797-807
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• 2008

For this study, the semi-adiabatic temperature rising test is accomplished by using ternary system cement (OPC, BFS, FA) reducing temperature crack. Two tests are conducted; one is for the paste test, and the other is for the concrete test. As the results of paste tests, using fly ash is better to reduce hydration temperature than doing blast furnace slag. In the case of the paste mixed ternary system cement, the more fly ash is mixed and the less blast furnace slag is used, the lower the temperature is. The less the mixture ratio of blast furnace slag is and the more the mixture ratio of fly ash is, the later the temperature rising velocity and descending velocity are. Besides, the temperature is lower if water/binder ratio is high. The use of ternary system cement has the retardation effect of temperature rising because the time to reach the maximum temperature is in the order of OPC100, binary system cement, and ternary system cement. From the test, the maximum temperature of concrete used ternary system cement is $8{\sim}11^{\circ}C$ lower than that of concrete used OPC100. Moreover, temperatures rising velocity and descending velocity of ternary system cement range $47{\sim}51%$ and $37{\sim}42%$ compared with OPC100. The specimen of concrete shows remarkable low internal temperature and slow temperature rising velocity and descending velocity compared with the specimen of paste because it is that temperature loss of concrete is much more than paste specimen according to aggregates.

• BMB Reports
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• v.35 no.3
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• pp.313-319
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• 2002

N-Acetyl-$\beta$-D-hexosaminidase ($\beta$-HexNAc'ase) (EC 3.2.1.52) was purified from rice seeds (Oryza sative L. var. Dongjin) using ammonium sulfate (80%) precipitation, Sephadex G-150, CM-Sephadex, and DEAE-Sephadex chromatography, sequentially. The activities were separated into 7 fractions($F_1-F_7$) by CM-Sephadex chromatography. Among them, F6 was further purified to homogeneity with a 13.0% yield and 123.3 purification-fold. The molecular mass was estimated to be about 52 kDa on SDS-PAGE and 37.4 kDa on Sephacryl S-300 gel filtration. The enzyme catalyzed the hydrolysis of both p-nitrophenyl-N-acetyl-$\beta$-D-hexosaminide (pNP-GlcNAc) and p-nitrophenyl-N-acetyl-$\beta$-D-hexosaminide (pNP-GalNAc) as substrates, which are typical properties of $\beta$-HexNAc'ase. The ratio of the pNP-GlcNAc'ase activity to the pNP-GalNAc'ase activity was 4.0. However, it could not hydrolyze chitin, chitosan, pNP-$\beta$-glucopyranoside, or pNP-$\beta$-glucopyranoside. The enzyme showed $K_m$, $V_{max}$ and $K_{cat}$ for pNP-GlcNAc of 1.65 mM, $79.49\;mM\;min^{-1}$, and $4.79{\times}10^6\;min^{-1}$, respectively. The comparison of kinetic values for pNP-GlcNAc and pNP-GalNAc revealed that the two enzyme activities are associated with a single binding site. The purified enzyme exhibited optimum pH and temperature for pNP-GlcNAc of 5.0 and $50^{\circ}C$, respectively. The enzyme activity for pNP-GlcNAc was stable at pH 5.0-5.5 and $20-40^{\circ}C$. The enzyme activity was completely inhibited at a concentration of 0.1 mM $HgCl_$ and $AgNO_3$, suggesting that the intact thiol group is essential for activity. Chloramine T completely inhibited the activity, indicating the possible involvement of methionines in the mechanism of the enzyme.

• BMB Reports
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• v.36 no.6
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• pp.545-551
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• 2003

A cDNA of bovine brain glutamate dehydrogenase (GDH) was isolated from a cDNA library by recombinant PCR. The isolated cDNA has an open-reading frame of 1677 nucleotides, which codes for 559 amino acids. The expression of the recombinant bovine brain GDH enzyme was achieved in E. coli. BL21 (DE3) by using the pET-15b expression vector containing a T7 promoter. The recombinant GDH protein was also purified and characterized. The amino acid sequence was found 90% homologous to the human GDH. The molecular mass of the expressed GDH enzyme was estimated as 50 kDa by SDS-PAGE and Western blot using monoclonal antibodies against bovine brain GDH. The kinetic parameters of the expressed recombinant GDH enzymes were quite similar to those of the purified bovine brain GDH. The $K_m$ and $V_{max}$ values for $NAD^+$ were 0.1 mM and $1.08\;{\mu}mol/min/mg$, respectively. The catalytic activities of the recombinant GDH enzymes were inhibited by ATP in a concentration-dependent manner over the range of 10 - $100\;{\mu}M$, whereas, ADP increased the enzyme activity up to 2.3-fold. These results indicate that the recombinant-expressed bovine brain GDH that is produced has biochemical properties that are very similar to those of the purified GDH enzyme.

• Journal of Korean Tunnelling and Underground Space Association
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• v.9 no.2
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• pp.133-141
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• 2007

The excavation damaged zone (EDZ) is an area around an excavation where in situ rock mass properties, stress condition, displacement, groundwater flow conditions have been altered due to the processes induced by the excavation. Various studies have been carried out on EDZ, but most studies have focused on the mechanical bahavior of EDZ by in situ experiment. Even though the EDZ could potentially form a high permeable pathway of groundwater flow, only a few studies were performed on the analysis of groundwater flow in EDZ. In this study, the 'hydraulic EDZ' was defined as the rock zone adjacent to the excavation where the hydraulic aperture has been changed due to the excavation by using H-M coupling analysis. Fundamental principles of distinct element method (DEM) were used in the analysis. In the same groundwater level, the behavior of hydraulic aperture near the cavern was analyzed for different stress ratios, initial apertures, fracture angles and fracture spacings by using a two-dimensional DEM program. We evaluate the excavation induced hydraulic aperture change. Using the results of the study, hydraulic EDZ was defined as an elliptical shape model perpendicular to the joint.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.6
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• pp.828-834
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• 2016

The aim of this study was to evaluate the physicochemical properties and antioxidant and anti-proliferative activities of different plant parts of Elaeagnus umbellata Thunb. extracted with ethanol (EtOH). EtOH extract of stems presenting the highest content of polyphenols showed the strongest 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity ($EC_{50}=54.04{\mu}g/mL$). The total content of free amino acids decreased in the order of leaves (6,179.12 mg/100 g)> stems (1,211.69 mg/100 g)> fruits EtOH extract (378.88 mg/100 g), and asparagine (1,907.57 mg/100 g), ${\gamma}-aminobutyric$ acid (300.17 mg/100 g), and proline (233.48 mg/100 g) were the major free amino acid in leaves, stems, and fruits, respectively. Five phenolic compounds in each extract were measured by using liquid chromatography- tandem mass spectrometry, and gallic acid (98.95 mg/100 g) and (+)-catechin (1,575.99 mg/100 g) were present as major components in leaves and stems, respectively. EtOH extract of leaves showed the highest anti-proliferative activity against HepG2 cells as measured by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazoliumbromide and lactate dehydrogenase assay but had no effects on Chang liver cells.

• Polymer(Korea)
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• v.30 no.1
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• pp.75-79
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• 2006

Silica nanoparticles for polymeric dental restorative composites were prepared by Stober method, and then the effects of surface treatment of silica particles with Lmethacrylofpropyltrimethofsilane $(\gamma-MPS)$ on the dispersity of the silica particles in the organic matrix was investigated. Particles having various average size were prepared by using controlled amounts of tetraethylorthosilicate(TEOS), water, and catalyst and by changing solvent used for reaction. The site of particles prepared by using methanol as solvent was smaller than that prepared by using ethanol as solvent. In addition, the size of particles was increased by decreasing amounts of water and by increasing amounts of TEOS and catalyst. Hydrophobic silica nanoparticles was prepared by reacting hydrophilic nanoparticles with $\gamma-MPS$ to improve interfacial properties with organic matrix. Amounts of $\gamma-MPS$ per unit mass of the particles was increased by decreasing particle size. even though the amount of $\gamma-MPS$ per specific surface area were nearly the same regardless of the particle size. The dispersity of the silica particles in the organic matrix was improved when the surface treated silica particles were used for preparing the polymeric dental restorative composites.

• Polymer(Korea)
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• v.27 no.5
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• pp.470-476
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• 2003

In order to obtain high refractive plastic materials, 1,2 -ethylenedisulfanylbis(2-mercaptomethyl-1-ethanthiol) (ESTT) was newly prepared in good yield by the reaction of 1,2-ethylenedisulfanylbis(2-bromomethyl-1-ethanthiol) (ESTB) with thiourea followed by hydrolysis using an aqueous ammonia solution and characterized by $^1$H-NMR (-SH at 1.7 ppm), $\^$13/C-NMR(-CH$_2$SH at 28.4 ppm) and FT-IR (-SH at 2540 cm$\^$-1/) spectroscopy, etc. Polythiourethanes (PTU) were obtained from the combinations of ESTT with each of 4,4＇-methylenebis(phenylisocyanate) (MDI), tolyene 2,4-diisocyanate (TDI), isophorone diisocyanate (IPDI), mxylene diisocyanate (XDI), and 1,6-diisocyanatohexane (HMDI) in the presence of dibutyltin dilaurylate as a catalyst, in a casting mold, and characterized by FT-IR (existence of N=C=O) spectroscopy and elemental analyzer (sulfur content). Accordingly, their thermal, mechanical and optical properties were investigated by using DSC, TGA, hardness tester and refractometer: both the melting point on DSC and crystallinity on X -ray diffraction (XRD) for specimens of PTUs were not observed. PTUs with T$\_$g/s above 110 $^{\circ}C$ showed good hardness (Shore D) in the range of 86 to 89. Thermal stabilities of PTUs obtained by using ESTT and each of diisocyanates containing aromatic rings were especially good. Also, the optical transmittances of amorphous PTUs through UV-visible source in the range of 400 to 600 nm were good. PTUs showed refractive indexes above 1.60, and their refractive indexes gradually increased with increase of sulfur contents.

• Membrane Journal
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• v.30 no.5
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• pp.319-325
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• 2020

In this study, we developed mixed matrix membranes by blending thermoplastic elastomer, i.e. polystyreneblock-polybutadiene-block-polystyrene (SBS) block copolymer with the synthesized UiO-66 particles for CO₂/N₂ gas separation. To investigate the effect of UiO-66 particles in the SBS matrix, we prepared different mixed matrix membranes (MMMs) by varying the mass ratio of SBS and UiO-66 in the blend. To fabricate well-dispersed UiO-66, the SBS/UiO-66 mixture was sonicated and stirred thoroughly. The physico-chemical properties of prepared membranes were characterized by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM). The gas separation performance was measured by time-lag method. The permeability of the MMMs increased significantly as the content of UiO-66 increased, but the CO₂/N₂ selectivity did not decrease significantly. The membranes containing 20% of UiO-66 particles showed the best performance with the CO₂ permeability and CO₂/N₂ selectivity of 663.8 barrer and 13.3, respectively. This result showed performance closer to upper bound than pure SBS membrane in the Robeson plot, as the added UiO-66 particles did not significantly sacrifice selectivity and more than doubled gas permeability.