• Mass Spectrometry Letters
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• v.11 no.4
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• pp.82-89
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• 2020

A systematic isolation and characterization study for Cassia auriculata (CA) seeds resulted in identifying antidiabetic compounds 1,3,8-trihydroxyanthraquinone and quercetin, quercetin-3-O-rutinoside, gallic acid, caffeic acid, ferulic acid, and ellagic acid. The ultra-high-performance liquid chromatography based triple quadrupole mass spectrometry methodology was developed and validated for simultaneous identification and confirmation of these compounds from CA seeds. Multiple reaction monitoring (MRM) based quantification method was developed with MRM optimizer software for MS1 and MS2 mass analysis. The method was optimized on precursor ions and product ions with the ion ratio of each compound. The calibration curves of seven bioactive analytes showed excellent linearity (r2 ≥ 0.99). The quantitation results found precise (RSD, < 10 %) with good recoveries (84.58 to 101.42%). The matrix effect and extraction recoveries were found within the range (91.66 to 102.11%) for the CA seeds. This is the first MS/MS-based methodology applied to quantifying seven antidiabetic compounds in CA seeds and its extract for quality control purposes.

• Mass Spectrometry Letters
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• v.2 no.2
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• pp.49-52
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• 2011

Competitive binding of $C_{60}$ and $C_{70}$ to meso-substituted porphyrins was studied by mass spectrometry (MS). Electrospray ionization MS was employed to acquire the mass spectra of 1 : 1 porphyrin-fullerene complexes formed in a mixture of mesosubstituted porphyrin and fullerite to determine the ratio of complexes between $C_{60}$ and $C_{70}$. Matrix-free laser desorption ionization MS was used to obtain the mass spectra of fullerite to measure the mole fraction of $C_{60}$ and $C_{70}$. The binding constant ratio ($K_{70}$/$K_{60}$) was determined from the mass spectral data. The difference in standard Gibbs free energy change, ${\Delta}({\Delta}G^o)_{70-60}$, for the competitive binding of $C_{60}$ and $C_{70}$ was calculated from $K_{70}$/$K_{60}$. Of the five porphyrins, tetraphenyl, tetra(4-pyridyl), tetra(4-carboxyphenyl), tetra(3,5-di-tert-butylphenyl), and tetra(pentafluorophenyl) porphyrins, the first three non-bulky porphyrins yield negative values of ${\Delta}({\Delta}G^o)_{70-60}$, whereas the other two bulky porphyrins result in positive values of ${\Delta}({\Delta}G^o)_{70-60}$. This result indicates that $C_{70}$ binding to porphyrin is thermodynamically favored over $C_{60}$ binding in non-bulky porphyrins, but disfavored in bulky ones. It also suggests that the binding mode of $C_{70}$is different between non-bulky and bulky porphyrins, which is in line with previous experimental findings of the "side-on" binding to non-bulky porphyrins and the $C_{60}$-like "end-on" binding to bulky porphyrins.

• Journal of Korean Society for Atmospheric Environment
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• v.19 no.6
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• pp.719-731
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• 2003

In order to maintain and manage ambient air quality, it is necessary to identify sources and to apportion its sources for ambient particulate matters. The receptor methods were one of the statistical methods to achieve reasonable air pollution strategies. Also, receptor methods, a field of chemometrics, is based on manifold applied statistics and is a statistical methodology that analyzes the physicochemical properties of gaseous and particulate pollutant on various atmospheric receptors, identifies the sources of air pollutants, and quantifies the apportionment of the sources to the receptors. The objective of this study was 1) after obtaining results from the PMF modeling, the existing sources of air at the study area were qualitatively identified and the contributions of each source were quantitatively estimated as well. 2) finally efficient air pollution management and control strategies of each source were suggested. The PMF model was intensively applied to estimate the quantitative contribution of air pollution sources based on the chemical information (128 samples and 25 chemical species). Through a case study of the PMF modeling for the PM-10 aerosols, the total of 11 factors were determined. The multiple linear regression analysis between the observed PM-10 mass concentration and the estimated G matrix had been performed following the FPEAK test. Finally the regression analysis provided quantitative source contributions (scaled G matrix) and source profiles (scaled F matrix). The results of the PMF modeling showed that the sources were apportioned by secondary aerosol related source 28.8 ％, soil related source 16.8％, waste incineration source 11.5%, field burning source 11.0%, fossil fuel combustion source 10%, industry related source 8.3％, motor vehicle source 7.9%, oil/coal combustion source 4.4％, non-ferrous metal source 0.3％. and aged sea- salt source 0.2％, respectively.

• Journal of Ocean Engineering and Technology
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• v.26 no.4
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• pp.86-91
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• 2012

A new identification method for a nonproportional damping matrix using the finite element (FE) model updating technique is proposed. Mass and stiffness matrices of the undamped system are identified by FE model updating method. Sensitivity analysis is used to update the FE model, and zero frequencies are considered as design parameters to supplement the information of vibration characteristics. The nonproportional damping matrix is identified through the proposed method. A numerical example is considered to verify the performance of the proposed method. As a result, the damping matrix of the nonproportional system is estimated accurately.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.23 no.1
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• pp.85-91
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• 2009

This paper proposes a simulation method of the internal winding fault to calculate the short-circuit current, electromagnetic force and vibration mode in a distribution power transformer by using FEM program(FLUX2D) and analytic algorithm. A usage of the Transfer matrix method is also presented for the vibration mode analysis of the cable-type winding of power transformer. The equations of the winding are approximated by the series expansions of the distributed mass mode and Timoshenko's beam theory. The simulation examples are provided for the cable type winding of the transformer(22.9[kV]/220[V], 1,000[kVA]) to verify the method. The proposed Transfer Matrix Method is also verified by the ANSYS program for the vibration mode of the transformer winding. The method presented may serve as one of the useful tools in the electromagnetic force and vibration analysis of the transformer winding under the short circuit condition.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2009.10a
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• pp.656-660
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• 2009

Various dynamic models of seated posture human body have been developed because the importance about the ride comfort assessment of vehicles is highly emphasized from day to day. The dynamic models of human body make possible the simulation of ride comfort assessment by applied to the vehicle dynamic model. Recently, the importance of ride comfort is also regarded to working vehicles such as excavators and the research of the ride comfort assessment for working vehicle is required. Only vertical vibration dominantly occurs on the seat of the private car driving with constant velocity. In contrast, vertical/fore-and-aft/pitch vibration seriously occurs on the seat of the working excavator. So, the dynamic models of seated human body applied to working vehicles should describe the dynamic characteristics for vertical/fore-and-aft/pitch direction. In this paper, the dynamic characteristics of seated human body are represented as apparent inertia matrix. The apparent inertia matrix is obtained by the vertical/fore-and-aft/pitch excitation of seated human body. 6 resonance frequencies are observed in apparent inertia matrix. This result can be applied to develop the dynamic model for seated posture human body.

• KSBB Journal
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• v.29 no.5
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• pp.380-384
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• 2014

Inhibition effects of peptide hydrolysates from Astragalus membranaceus Bunge. on the expression of the matrix metalloproteinases (MMPs) in human dermal fibroblasts were evaluated in vitro. Crude peptides were obtained by the hydrolysis of proteins extracted from A. membranaceus. Peptides were purified partially by the basis on the molecular weight using 40% polyacrylamide gel electrophoresis before treatment with human dermal fibroblasts. Basis on the doseeffect experiments, expressions of MMPs including MMP-1, MMP-3, MMP-8, MMP-13 in human dermal fibroblasts were evaluated. Expressions of MMP-1, MMP-3, MMP-8 and MMP-13 were reduced in 43%, 5%, 22% and 57% respectively. The mass spectrometric analysis of partially purified peptides from A. membranaceus, which strongly inhibit expressions of MMPs, indicated that the peptides were composed of molecules below 1500 Da.

• Analytical Science and Technology
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• v.7 no.1
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• pp.1-10
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• 1994

A survey was made on detection limit, reproducibility, matrix effect, linear dynamic range and the memory effect of yttrium and europium in order to analyze rare earth elements which exist as impurities in the rare earth oxide which is raw materials of fluorescent substance. When analysing a certain amount of thulium quantitatively using inductively coupled plasma mass spectrometry, it was found that the analysis was interfered with $EuO^+$ which is one of polyatomic ions caused by plasma. As the intensity of thulium linearly proportional to the europium concentration, it was possible to the determine the actual concentration of thulium.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.3
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• pp.463-472
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• 1994

A method of stochastic response analysis of base-isolated fluid-filled pool structures subject to random ground excitations is studied. Fluid-structure interaction effects between the flexible walls and contained fluid are taken into account in the form of added mass matrix derived by FEM modeling of the contained fluid motion. The stationary ground excitation is represented by Modified Clough-Penzien spectral model and the nonstationary one is obtained by imposing an envelope function on the stationary one. The stationary and nonstationary response statistics of the two different isolation systems are obtained by solving the governing Lyapunov covariance matrix differential equations.

• Korean Institute of Interior Design Journal
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• v.14 no.1
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• pp.37-44
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• 2005

Currently, the space design has been expressed the space in kinetic design by digital technology. To look into the concept of digital design, there is the tendency to pursue the harmony of the nature. The digital space designer, Greg Lynn who has been paid attention by international researchers. To compared with the reputation of his works, the information about him has been limited to us. The purpose of this study was to investigate the Greg Lynn's digital design matrix toward the design process in his representative 11 works in his website; www.glform.com. The contents analyses methods were used in this study. Greg Lynn's internet website survey was carried out in the respects of thinking method, space formative language and animate form. The major results of this study are as follows: \circled1 Lynn's design concept and digital methodology were affected by Paolo Soleri and Peter Eisenman: natural architectural concept and digital animate form \circled2 Lynn's space formative languages were 10 items; blob, blob, fold, strand, shred, flower, skin, teeth, branch and lattice \circled3 Lynn's digital design matrix was divided into 3 types; MS(Mass + Structure), PC(Path + Circulation) and FD(Form + Detail) \circled4 According to the analysis of longitudinal, his works have been changed from the MS and PC to FD. This research will be a basic reference to understand digital space design.