• Bulletin of the Korean Chemical Society
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• v.31 no.8
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• pp.2293-2298
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• 2010

Focused electrospray (FES) deposition method is presented for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. FES ion optics consists of two cylindrical focusing electrodes capped with a truncated conical electrode through which an electrospray emitter passes along the cylindrical axis. A spray of charged droplets is focused onto a sample well on a MALDI target plate under atmospheric pressure. The shape and size distributions of matrix crystals are visualized by scanning electron microscope and the mass spectra are obtained by time-of-flight mass spectrometry. Angiotensin II, bradykinin, and substance P are used as test samples, while $\alpha$-cyano-4-hydroxycinnamic acid and dihydroxybenzoic acid are employed as matrices. FES of a sample/matrix mixture produces fine crystal grains on a 1-3 mm spot and reproducibly yields the mass spectra with little shot-to-shot and spot-to-spot variations. Although FES greatly stabilizes the signals, the space charge due to matrix ions limits the detection sensitivity of peptides. To avoid the space charge problem, we adopted a dual FES/FES mode, which separately deposits matrix and sample by FES in sequence. The dual FES/FES mode reaches the detection sensitivity of 0.88 amol, enabling ultrasensitive of peptides by homogeneously depositing matrix and sample under atmospheric pressure.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2004.04a
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• pp.1-8
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• 2004

For the linearized differential algebraic equation of the nonlinear constrained system, exact initial values of the acceleration are needed to solve itself. It may be very troublesome to perform the inverse operation for obtaining the incremental quantities since the mass matrix contains the zero element in the diagonal. This fact makes the mass matrix impossible to be positive definite. To overcome this singularity phenomenon the mass matrix needs to be modified to allow the feasible application of predictor and corrector in the iterative computation. In this paper the proposed numerical algorithm based on the modified mass matrix combines the conventional implicit algorithm, Newton-Raphson method and Newmark method. The numerical example presents reliabilities for the proposed algorithm via comparisons of the 4th order Runge-kutta method. The proposed algorithm seems to be satisfactory even though the acceleration, Lagrange multiplier, and energy show unstable behaviour. Correspondingly, it provides one important clue to another algorithm for the enhancement of the numerical results.

• Analytical Science and Technology
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• v.8 no.4
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• pp.465-468
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• 1995

Matrix assisted laser desorption ionization in mass spectrometry is a fast and accurate method to determine the molecular weight of natural and synthetic polymers. Unknown peptides such as elastase inhibitor and $\small{D}$-hydantoinase were analyzed using sinapinic acid as matrix and their molecular weights were compared with the results from protein sequencer and gel filtration chomatography, respectively. Synthetic polymers such as polyethyleneglycol, polypropyleneglycol, polydimethylsiloxane, and polystyrene were analyzed using matrices such as 2,5-dihydroxybenzoic acid, 4-hdroxyazobenzenecarboxylic acid, and 2-nitrophenyl octyl ether. Average molecular weights of polystyrene were compared with molecular weights by gel permeation chromatography.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 1999.04a
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• pp.275-283
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• 1999

To predict the dynamic behavior of the cylindrical liquid storage tank subjected to seismic ground motion three dimesional analysis with liquid-structure interaction must be performed, In this study a three dimensional dynamic analysis method over the frequency domain using FE-BE coupling technique which combines the efficiency of the boundary elements for liquid with the versatility of the finite shell elements for tank. The liquid region is modeled using boundary elements which can counter the sloshing effect at free surface and the structure region the tank itself is modeled using the degenerated finite shell elements. At the beginning of the procedure the equivalent mass matrix of the liquid is generated by boundary elements procedure. Then this equivalent mass matrix is combined with the mass matrix of the structure to produce the global mass matrix in the equation of the motion of fluid-structure interaction problem In order to demonstrate the accuracy and validity of the developed method the numerical results re compared with the previous studies. Finally the effects of the fluid-structure interaction on the natural frequency and dynamic response of the system are analyzed.

• Mass Spectrometry Letters
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• v.5 no.2
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• pp.49-51
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• 2014

The effectiveness of tertiary matrices composed of the combination of three common matrices (dihydrobenzoic acid (DHB), ${\alpha}$-cyano-4-hydroxycinnamic acid (CHCA), and sinapinic acid (SA)) was compared with that of single or binary matrices in the analysis of polyethylene glycol (PEG) polymers ranging from 1400 to 10000 Da using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A tertiary matrix of 2,5-DHB+CHCA+SA was the most effective in terms of S/N ratios. CHCA and CHCA+SA produced the highest S/N ratios among the single matrices and the binary matrices, respectively. The improvement observed when using a tertiary matrix in analyses of PEG polymers by MALDI-TOF MS is believed to be due to the uniform morphology of the MALDI sample spots and synergistic effects arising from the mixture of the three matrix materials.

• Journal of the Korean Society for Precision Engineering
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• v.22 no.1
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• pp.143-151
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• 2005

This paper study the effect of open cracks on the dynamic behavior of simply supported Timoshenko beam with a moving mass. The influences of the depth and the position of the crack in the beam have been studied on the dynamic behavior of the simply supported beam system by numerical method. Using Lagrange's equation derives the equation of motion. The crack section is represented by a local flexibility matrix connecting two undamaged beam segments i.e. the crack is modeled as a rotational spring. This flexibility matrix defines the relationship between the displacements and forces on the crack section and is derived by the applying fundamental fracture mechanics theory. As the depth of the crack is increased the mid-span deflection of the Timoshenko beam with the moving mass is increased. And the effects of depth and position of crack on dynamic behavior of simply supported beam with moving mass are discussed.

• The Korean Journal of Cytopathology
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• v.8 no.2
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• pp.174-178
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• 1997

Matrix producing carcinoma of the breast is a variant of heterologous metaplastic carcinoma which is defined as "overt carcinoma with direct transition to a cartilaoenous and/or osseous stromal matrix without an intervening spindle cell zone or osteoclastic cells". This tumor is very rare, occuring in less than 0.2% of total breast carcinoma, but the prognosis is better than other metaplastic carcinoma. We experienced a case of fine needle aspiration(FNA) cytologic finding of matrix producing carcinoma of the breast. A 75-year old woman, who presented a right huge breast mass$(9{\times}8cm)$ during 10months, was examined. Mammography reveals right lateral mass with even density without calcification. Breast ultrasonography shows multifocal hypoechogenic cystic change in the huge mass, suggesting resolving hematoma or carcinoma or sarcoma with necrosis. On cytologic finding of FNA, myxoid matrix was the dominant feature and the rest of the material was composed of scanty isolated atypical cells with large irregular nuclei. The histologic finding was moderately differentiated adenocarcinoma with abundant cartilagenous matrix and focal squamous metaplasia.

• Structural Engineering and Mechanics
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• v.19 no.5
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• pp.551-566
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• 2005

Using two different, but related approaches, an exact dynamic stiffness matrix for a two-part beam-mass system is developed from the free vibration theory of a Bernoulli-Euler beam. The first approach is based on matrix transformation while the second one is a direct approach in which the kinematical conditions at the interfaces of the two-part beam-mass system are satisfied. Both procedures allow an exact free vibration analysis of structures such as a plane or a space frame, consisting of one or more two-part beam-mass systems. The two-part beam-mass system described in this paper is essentially a structural member consisting of two different beam segments between which there is a rigid mass element that may have rotatory inertia. Numerical checks to show that the two methods generate identical dynamic stiffness matrices were performed for a wide range of frequency values. Once the dynamic stiffness matrix is obtained using any of the two methods, the Wittrick-Williams algorithm is applied to compute the natural frequencies of some frameworks consisting of two-part beam-mass systems. Numerical results are discussed and the paper concludes with some remarks.

• International Journal of Fluid Machinery and Systems
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• v.5 no.3
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• pp.126-133
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• 2012

The transfer matrix and unsteady cavitation characteristics, cavitation compliance and mass flow gain factor, of cavitating inducer were evaluated by CFD using commercial software. Quasi-steady values of cavitation compliance and mass flow gain factor were obtained first by using steady calculations at various flow rate and inlet cavitation number. Then unsteady calculations were made to determine the transfer matrix and the cavitation characteristics. The results are compared with experiments to show the validity of calculations.

• Mass Spectrometry Letters
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• v.1 no.1
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• pp.33-36
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• 2010

Wood charcoal was investigated to determine its potential as an alternative matrix for matrix-assisted laser desorption/ionization of various samples. Wood charcoal was an effective matrix for analyzing glucose, sucrose, arginine, and polyethylene glycols (PEGs), with detection levels of 100 pmol for glucose, 1 nmol for sucrose, 100 pmol for arginine, 100 pmol for PEG 400, 1 pmol for PEG 1540, and 10 pmol for PEG 3350. No analyte signal was observed for peptides or proteins.