• Mass Spectrometry Letters
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• v.6 no.1
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• pp.21-25
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• 2015

In this paper, I describe the importance of matrix spraying conditions in imaging mass spectrometry (IMS) to obtain successful imaging results. My developed matrix application methodology, which is a "two-step matrix application" sequentially combined with matrix sublimation and spraying matrix solution can provide high reproducibility and high ion yield compared with a conventional direct spraying method. However, insufficient IMS results were obtained occasionally despite the two-step method. Therefore, I wanted to characterize the methodology to continuously provide high quality data. According to my results, the sublimation time was not a strict parameter, and the most important step was the first spraying condition. This means that the extraction conditions from the tissue section and co-crystallization of the matrix were the most important factors.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.21 no.2
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• pp.288-296
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• 1997

Curved beam elements with two nodes based on shallow beam geometry and strain interpolations are employed in eigenvalue analysis. In these elements, the displacement interpolation functions and mass matrices are consistent with strain fields. To assess the quality of the element mass matrix in free vibration problems, several numerical experiments are performed. In these analysis, both the inconsistent mass matrices using linear displacement interpolation function and the consistent mass matrices are used to show the difference. The numerical results demonstrate that the accuracy is closely related to the property of the mass matrix as well as that of the stiffness matrix and that the mass matrix consistent with strain fields is very beneficial to eigenvalue analysis. Also, it is proved that the strain based elements are very efficient in a wide range of element aspect ratios and curvature properties.

• Transactions of the Korean Society of Mechanical Engineers
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• v.15 no.2
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• pp.445-454
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• 1991

In this thesis, Mindlin plate element with nine nodes and three degrees-of-freedom at each node is formulated and is employed in eigen-analysis of a rectangular plates in order to alleviate locking phenomenon of eigenvalues. Eigenvalues and their modes may be locked if conventional $C_{0}$-isoparametric element is used. In order to reduce stiffness locking phenomenon, two methods (1, the general reduced and selective integration, 2, the new element that use of modified shape function) are studied. Additionally in order to reduce the error due to mass matrix, two mass matrixes (1, Gauss-Legendre mass matrix, 2, Gauss-Lobatto mass matrix) are considered. The results of eigen-analysis for two models (the square plate with all edges simply-supported and all edges built-in), computed by two methods for stiffness matrix and by two mass matrixes are compared with theoretical solutions and conventional numerical solutions. These comparisons show that the performance of the two methods with Gauss-Lobatto mass matrix is better than that of the conventional plate element. But, by considering the spurious rigid body motions, the element which employs modified shape function with full integration and Gauss-Lobatto mass matrix can elevate the accuracy and convergence of numerical solutions.

• Bulletin of the Korean Chemical Society
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• v.35 no.12
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• pp.3482-3488
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• 2014

Matrix-induced mass bias and its effect on the accuracy of isotope ratio measurements have been examined for a quadrupole-based inductively coupled plasma-mass spectrometer (Q ICP-MS). Matrix-induced mass bias effect was directly proportional to % mass difference, and its magnitude varied for element and nebulizer flow rate. For a given element and conditions in a day, the effect was consistent. The isotope ratio of Cd106/Cd114 under $200{\mu}g\;g^{-1}$ U matrix deviated from the natural value significantly by 3.5%. When Cd 111 and Cd114 were used for the quantification of Cd with isotope dilution (ID) method, the average of differences between the calculated and measured concentrations was -0.034% for samples without matrix ($0.076{\mu}g\;g^{-1}$ to $0.21{\mu}g\;g^{-1}$ for the period of 6 months). However, the error was as large as 1.5% for samples with $200{\mu}g\;g^{-1}$ U. The error in ID caused by matrix could be larger when larger mass difference isotopes are used.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.19 no.10
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• pp.1021-1027
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• 2009

Finite element models of dynamic systems can be updated in two stages. In the first stage, mass and stiffness matrices are updated neglecting damping. In the second stage, a damping matrix is estimated with the mass and stiffness matrices fixed. Methods to estimate a damping matrix for this purpose are proposed in this paper. For a system with proportional damping, a damping matrix is estimated using the modal parameters extracted from the measured responses and the modal matrix calculated from the mass and stiffness matrices from the first stage. For a system with non-proportional damping, a damping matrix is estimated from the impedance matrix which is the inverse of the FRF matrix. Only one low or one column of the FRF matrix is measured, and the remaining FRFs are synthesized to obtain a full FRF matrix. This procedure to obtain a full FRF matrix saves time and effort to measure FRFs.

• Mass Spectrometry Letters
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• v.4 no.2
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• pp.38-40
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• 2013

Glycans released from ovalbumin by PNGase F were analyzed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry using three different dihydroxybenzoic acid (DHB) matrix systems: 2,5-DHB, 2,6-DHB, and a 2,5-DHB/2,6-DHB binary matrix. Relative to the results obtained with the single-component matrices (2,5-DHB or 2,6-DHB), the 2,5-DHB/2,6-DHB binary matrix boasted lower background noise and higher sensitivity. A total of 16 glycan peaks were observed using the 2,5-DHB/2,6-DHB binary matrix, while only 10 and 9 glycan peaks were observed using the 2,5-DHB and 2,6-DHB matrices, respectively.

• BMB Reports
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• v.53 no.7
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• pp.349-356
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• 2020

Mass spectrometry (MS) is an ideal tool for analyzing multiple types of (bio)molecular information simultaneously in complex biological systems. In addition, MS provides structural information on targets, and can easily discriminate between true analytes and background. Therefore, imaging mass spectrometry (IMS) enables not only visualization of tissues to give positional information on targets but also allows for molecular analysis of targets by affording the molecular weights. Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) MS is particularly effective and is generally used for IMS. However, the requirement for an organic matrix raises several limitations that get in the way of accurate and reliable images and hampers imaging of small molecules such as drugs and their metabolites. To overcome these problems, various organic matrix-free LDI IMS systems have been developed, mostly utilizing nanostructured surfaces and inorganic nanoparticles as an alternative to the organic matrix. This minireview highlights and focuses on the progress in organic matrix-free LDI IMS and briefly discusses the use of other IMS techniques such as desorption electrospray ionization, laser ablation electrospray ionization, and secondary ion mass spectrometry.

• Journal of Photoscience
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• v.8 no.2
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• pp.83-86
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• 2001

Structural analyses of oligosaccharide-malononitrile derivatives were conducted by matrix-assisted laser desorption/ionization post-source decay (MALDI-PSD) analysis in positive ion mode. The malononitrile derivatives of oligosaccharides, which were developed for highly sensitive detection of multi-component oligosaccharides by negative ion electrospray ionization mass spectrometry (ESI MS), were detected by positive-ion MALDI with the detection limit of 2 pmol level from the crude derivatization sample. The used matrix affected drastically the analytical results of oligosaccharide-malononitrile derivative by matrix-assisted laser desoprtion/ionization mass spectrometry (MALDI MS). The malononitrile derivatization of oligosaccharide also affect the patterns of MALDI-PSD spectra and give much more structural information than the free oligosaccharide.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.15 no.4 s.97
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• pp.483-491
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• 2005

In this paper a dynamic behavior of a double-cracked cantilever pipe with the tip mass and a moving mass is presented. Based on the Euler-Bernoulli beam theory, the equation of motion is derived by using Lagrange's equation. The influences of the moving mass, the tip mass and double cracks have been studied on the dynamic behavior of a cantilever pipe system by numerical method. The cracks section are represented by the local flexibility matrix connecting two undamaged beam segments. Therefore, the cracks are modelled as a rotational spring. This matrix defines the relationship between the displacements and forces across the crack section and is derived by applying fundamental fracture mechanics theory. We investigated about the effect of the two cracks and a tip mass on the dynamic behavior of a cantilever pipe with a moving mass.

• Ceramist
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• v.21 no.3
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• pp.249-269
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• 2018

In conventional MALDI-TOF mass spectrometry, analyte molecules are known to be ionized by mixing with organic matrix molecules. As the organic matrix molecules are made into small fragments, they generate unreproducible mass peaks such that MALDI-TOF mass spectrometry is nearly impossible in the low mass-to-charge (m/z) range (< 1000). Additionally, the dried sample mixed with matrix were made as inhomogeneous crystal on metal plate. When the laser radiation was made on the sample crystal, the amount of generated sample ion was observed to be quite different according to the radiation point. Therefore, the quantitative analysis was very difficult even for the sample spots at the same concentration for the conventional MALDI-TOF mass spectrometry. In this work, we present laser desorption/ionization (LDI) mass spectrometry based on solid-matrices for the quantitative analysis of small molecules in the low m/z range by using MALDI-TOF mass spectrometry: (1) Carbon based nanostructures; (2) Semiconductor based nanomaterials; (3) Metal based nanostructures.