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http://dx.doi.org/10.5478/MSL.2013.4.1.38

Enhanced Detection of Glycans by MALDI-TOF Mass Spectrometry Using a Binary Matrix of 2,5-Dihydroxybenzoic Acid and 2,6-Dihydroxybenzoic Acid  

Kim, Yunjin (Department of Chemistry, Chungnam National University)
Kim, Taehee (Department of Chemistry, Chungnam National University)
Lee, Jihyeon (Department of Chemistry, Chungnam National University)
Im, Haeju (Department of Chemistry, Chungnam National University)
Kim, Jeongkwon (Department of Chemistry, Chungnam National University)
Publication Information
Mass Spectrometry Letters / v.4, no.2, 2013 , pp. 38-40 More about this Journal
Abstract
Glycans released from ovalbumin by PNGase F were analyzed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry using three different dihydroxybenzoic acid (DHB) matrix systems: 2,5-DHB, 2,6-DHB, and a 2,5-DHB/2,6-DHB binary matrix. Relative to the results obtained with the single-component matrices (2,5-DHB or 2,6-DHB), the 2,5-DHB/2,6-DHB binary matrix boasted lower background noise and higher sensitivity. A total of 16 glycan peaks were observed using the 2,5-DHB/2,6-DHB binary matrix, while only 10 and 9 glycan peaks were observed using the 2,5-DHB and 2,6-DHB matrices, respectively.
Keywords
Glycans; MALDI; PNGase F; ovalbumin; oligosaccharides; dihydroxybenzoic acid;
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