• 한국의류학회지
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• 제26권9호
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• pp.1308-1319
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• 2002

The purpose of this is to analyze the aesthetics characteristics of our modern sports fashion, and thereby, discuss them in the light of the overall mentality or the 20th century, and thus, present the conditions or sports fashion design meeting modem people's divers aesthetics values and desired. Modern sports fashion sues can be categorized into futurist sports style, erotic sports style and American sheet sports style. And the 20th century mentality characterized by changes of lifestyle, identity and aspiration has influenced the aesthetic features of such sports fashion sues, which can be summed up as follows; first, the futurist sports style applies the functional items and details of active sportswear to design, while heralding a positive and hopeful message of technology and future by using the material of hi-tech functions and senses. This sports style was affected much by shift from social status, attraction and wealth to demonstration of state-of-the-art science, pursuit of functionality in terms of shapes and materials, convenience through See combinations of sportswear items or design elements. Second, the erotic sports style based on minimalism attempted to express the erotic body beauty indirectly by exposing some parts of body or using the material pressed against the body. This sports style was closely related with the changes of sexual identity such as neutral sexualism, bi-sexualism and homo-sexualism. Lastly, the American street sports style was born from black Americarns' sports and dances. This spors style pursues “youth” beyond TPO concept As mass media and commercial sports developed, the young generation copied sports stars' uniforms or fashions to share honor, wealth and youth with them. In sort, the American sheet sports style was affected much by the so-called “heroism”. Such a changed object of aspiration influenced the aesthetic characteristics of American shot sports style directly.

• BMB Reports
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• 제44권1호
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• pp.52-57
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• 2011

Termites play an important role in the degradation of dead plant materials and have acquired endogenous and symbiotic cellulose digestion capabilities. The feruloyl esterase enzyme (FAE) gene amplified from the metagenomic DNA of Coptotermes formosanus gut was cloned in the TA cloning vector and subcloned into a pET32a expression vector. The Ft3-7 gene has 84% sequence identity with Clostridium saccharolyticum and shows amino acid sequence identity with predicted xylanase/chitin deacetylase and endo-1,4-beta-xylanase. The sequence analysis reveals that probably Ft3-7 could be a new gene and that its molecular mass was 18.5 kDa. The activity of the recombinant enzyme (Ft3-7) produced in Escherichia coli (E.coli) was 21.4 U with substrate ethyl ferulate and its specific activity was 24.6 U/mg protein. The optimum pH and temperature for enzyme activity were 7.0 and $37^{\circ}C$, respectively. The substrate utilization preferences and sequence similarity of the Ft3-7 place it in the type-D sub-class of FAE.

• 한국산학기술학회논문지
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• 제16권10호
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• pp.6715-6721
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• 2015

최근 실종자, 변사자, 미아 등의 유전자 분석 자료는 지속적으로 증가하고 있으나, 현재 친자확인을 위한 통계학적 계산은 대부분 수기에 의하거나 엑셀을 통해서 이루어지고 있다. 따라서 유전자 분석 자료 중 상염색체 Short Tandem Repeat (STR)을 체계적으로 관리하고 효과적으로 분석할 수 있는 소프트웨어의 개발이 필요하다. 친자관계 및 혈연관계를 다양한 옵션 하에서 용이하게 분석하는 웹 기반 유전자자료 분석시스템이 광범위한 테스트 없이 약 20개월의 연구를 통해서 개발되었다. 친자관계 분석을 위해서 부계지수 계산 알고리즘을 사용하였고, 혈연관계 분석을 위해서 Identity by descent (IBD) 공식을 사용하였다. 이 시스템은 실제 데이터를 기반으로 혈연관계지수와 친자확률이 검증됨으로써 신뢰성이 확보됨은 물론, 대량 재난 재해 시 발생될 유전자 분석 자료의 관리 및 분석에 효과적으로 이용될 수 있을 것이다. 이 외에도 본 시스템은 데이터베이스와 알고리즘의 통합 환경, 사용자 중심 인터페이스, 프로세스 자동화 등 고급기능을 포함한다.

• Applied Biological Chemistry
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• 제31권3호
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• pp.274-283
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• 1988

Aqualysin I is an alkaline serine protease which is secretet into the culture medium by Thermus aquaticus YT-1, an extreme thermophile. Aqualysin I was purified, and its partial amino acid sequence was determined. The gene encoding aqualysin I was cloned into E. coli using synthetic oligodeoxyribonucleotides as hybridization probes. The nucleotide sequence of the cloned DNA was determined. The primary structure of aqualysin I, deduced from the nucleotide sequenc, agreed with the determid amino acid sequences, including the $NH_2-$ and COOH terminal sequence of the tryptides derived from aqualysin I. Aqualysin I comprised 281 amino acid residues and its molecular mass was determined to be 28350. On alignment of the whole amino acid sequence, aqualysin I showed high sequence homology with the subtilisin type serine protease, and 43% identity with proteinase K, 37-30% with subtilisins and 34% with thermitase. Extremely high sequence identity was observed in the regions containing the active-site residues, corresponding to Asp32, His64 and Ser221 of subtilisin BPN'. Aqualysin I contains two disulfide bonds, Cys67-Cys99 and Cys163-Cys194, and these disulfide bonds seem to contribute to the heat stability of the enzyme. The determined positions of the twe disulfide bonds of aqualysin I agreed with those predicted previously on the basis of computer graphics of the crystallographic data for subtilisin BPN'. Therefore, these findings sugests that the three-dimensional structure of aqualysin I is similar to that of subtilisin BPN' Aqualysin I is produced as a lage precursor, which contains $NH_2-$ and COOH- terminal portions besides the mature protease sequence.

• 한국실내디자인학회논문집
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• 제15권3호
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• pp.92-101
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• 2006

This study examines placeness of the living space on the basis of Schulz's existential space and inquires into the expressional features of placeness by analyzing cases. Results of this study have shown that placeness of living space is formed by three factors as follows. First, the living space with placesness maintains inner order which structuralizes surroundings. Second, it expresses its identity through innate shape that reflects surroundings. Third, inner space has innate identity and it is much related to characteristics personality of a resident, environmental and psychological factors. It is as follows that concrete features of existential placeness shown in analyzing cases of Botta and Moore's works. There are concrete expressional features of placeness in the housing of Botta, and one is to keep order of inner space the horizontal and vertical axis reflected surroundings. Another is to show existence feeling as the shape of a stable singular mass with surroundings and regional properties. The third is to value innate features of each space inside housing and particularly to acquire placeness as combining phenomenological characteristic of light. There are concrete expressional features of placeness in the housing of Moore, and first, strong centrality formed in the inside is emphasized as extending to outside environment. Second, existence feeling is acquired as familiar form using the shape and material considered surroundings. Third, the personality of a resident is positively reflected in the design. Besides, placeness is acquired by goods and furniture as positively considering environmental and psychological sides.

• Journal of Applied Biological Chemistry
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• 제62권1호
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• pp.73-79
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• 2019

A novel esterase gene (est7S) was cloned from an enriched metagenomic library derived from an oil-spill area. The gene encoded a protein of 505 amino acids, and the molecular mass of the Est7S was estimated to be 54,512 Da with no signal peptide. Est7S showed the highest identity of 40% to an esterase from a sludge metagenome compared to the characterized enzymes with their properties, although it showed 99% identity to a carboxylesterase in the genome sequence of Alcanivorax borkumensis SK2. Est7S had catalytic triad residues, Ser183, Glu312, and His420, and the GESAG motif in most family VII lipolytic enzymes. Est7S was purified from the crude extract of clone SM7 using Sephacryl S-200 HR and HiTrap Q column chromatographies. The purified Est7S was optimally active at $50^{\circ}C$ and pH 10.0. Est7S showed a high specific activity of 366.7 U/mg protein. It preferred short length esters, particularly p-nitrophenyl acetate, efficiently hydrolyzed R- and S-enantiomers of methyl-3-hydroxy-2-methylpropionate, and glyceryl tributyrate. These properties of Est7S may provide potential merits in biotechnological applications such as detergent and paper processing under alkaline conditions.

• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.272-279
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• 2021

Two genes encoding probable α-ʟ-arabinofuranosidase (E.C. 3.2.1.55) isozymes (ABFs) with 92.3% amino acid sequence identity, ABF51A and ABF51B, were found from chromosomes 3 and 5 of Saccharomycopsis fibuligera KJJ81, an amylolytic yeast isolated from Korean wheat-based nuruk, respectively. Each open reading frame consists of 1,551 nucleotides and encodes a protein of 517 amino acids with the molecular mass of approximately 59 kDa. These isozymes share approximately 49% amino acid sequence identity with eukaryotic ABFs from filamentous fungi. The corresponding genes were cloned, functionally expressed, and purified from Escherichia coli. SfABF51A and SfABF51B showed the highest activities on p-nitrophenyl arabinofuranoside at 40~45℃ and pH 7.0 in sodium phosphate buffer and at 50℃ and pH 6.0 in sodium acetate buffer, respectively. These exoacting enzymes belonging to the glycoside hydrolase (GH) family 51 could hydrolyze arabinoxylo-oligosaccharides (AXOS) and arabino-oligosaccharides (AOS) to produce only ʟ-arabinose, whereas they could hardly degrade any polymeric substrates including arabinans and arabinoxylans. The detailed product analyses revealed that both SfABF51 isozymes can catalyze the versatile hydrolysis of α-(1,2)- and α-(1,3)-ʟ-arabinofuranosidic linkages of AXOS, and α-(1,2)-, α-(1,3)-, and α-(1,5)-linkages of linear and branched AOS. On the contrary, they have much lower activity against the α-(1,2)- and α-(1,3)-double-substituted substrates than the single-substituted ones. These hydrolases could potentially play important roles in the degradation and utilization of hemicellulosic biomass by S. fibuligera.

• 한국지역지리학회지
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• 제14권1호
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• pp.40-53
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• 2008

이 연구는 국제결혼이주여성이 노동자에서 배우자, 어머니, 시민이라는 사회적 위치의 변화 과정에 따라 자신들의 정체성과 주체성도 달라지고 있음을 구미지역에 거주하는 8명의 이주여성을 대상으로 심층 인터뷰 방법으로 분석하였다. 본 연구는 이주여성들의 생애사적 체험을 토대로 개인적인 삶을 재구성하고 해석하는 과정을 중심으로 살펴보았다. 사례분석 결과, 이주여성들은 미혼여성노동자, 배우자, 어머니, 시민이라는 자신들이 처한 사회적 위치성에 따라 자신의 정체성이 유동되어짐을 보여주었다. 사회적 위치성은 이주라는 공간이동을 통하여 발생하는 새로운 영역과 사회적 관계를 동시에 보여준다. 본국의 과거성에서 출발하는 자신의 정체성은 정착국의 상황, 조건에 따라 변화하는 모습을 보이고 있었다. 결혼이주여성이 정책의 대상이나 매스컴의 대상으로 부각되면서 상대적으로 이주여성을 어려운 생활에 있는 피해자나 빈곤한 나라에서 온 무지한 자로 자신들을 상정하는 것에 결혼이주여성은 강한 거부감을 표시하였다. 이것은 주체적으로 선택한 국제결혼에 대한 이중적인 감정이 나타나고 있음을 보여준다. 그러나 정부정책으로 인한 결혼이민자가족지원센터라는 공간의 형성은 이들에게 집단적 여성주체로 설 수 있는 단초를 제공해 주었다. 그 결과 국제결혼이주여성을 결혼하기 위해 이주해 온 단일그룹으로서가 아닌 행위 주체로서의 이주여성들에 대한 다양하고 복잡한 상황들을 반영할 수 있는 연구들이 요구된다.

• 한국언론정보학보
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• 제62권
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• pp.30-50
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• 2013

이 연구는 젠더연구에서 몸과 테크놀로지가 가지는 의미를 고찰함으로써, '여성'이라는 범주가 고정된 것이 아니라 '여성되기'의 진행적인 과정임을 제시한다. 신자유주의 시대에 젠더 정체성은 여성과 테크놀로지의 관계를 재설정해야 할 여러 가지 문제들을 제시하고 있으며, 이 관계의 역동성을 이해하기 위해서는 '몸'의 문제를 살펴볼 필요가 있다. 이를 위해서 본 연구는 먼저 페미니즘 연구와 젠더 연구 전반에서 몸에 대한 담론이 진행되어온 과정과 의미를 탐색했다. 이어서 몸과 젠더의 관계에서 테크놀로지가 갖는 위치를 살펴보고, 몸이 젠더 정체성을 주체적으로 수행하는 동시에 정체성이 구성되기도 하는 담론의 장이자 물질적 장소임을 주장했다. 마지막으로는 몸과 젠더와 테크놀로지의 삼자관계에 집중한 사이버페미니즘의 논의를 구체적으로 살펴봄으로써, '여성되기'에서 테크놀로지가 갖는 역할과 의미를 재고해야 할 필요성에 대해 논의했다. 결국 여성되기에 있어서 테크놀로지가 갖는 의미를 살펴보는 일은, 일상의 경험 세계에서 몸이 지니는 담론적이고 실천적인 의미에 대한 숙고를 통해 이루어져야 함을 제시했다. '여성되기'의 가능성은, 몸-젠더-테크놀로지의 접합방식과 과정에 대한 탐구를 통해 젠더의 (재)구성을 살펴볼 때만 상상될 수 있을 것이다.

• BMB Reports
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• 제40권5호
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• pp.832-838
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• 2007

A novel inhibitory protein against blood coagulation factor Va (FVa) was purified from muscle protein of granulated ark (Tegillarca granosa, order Arcoida, marine bivalvia) by consecutive FPLC method using anion exchange and gel permeation chromatography. In the results of ESI-QTOF tandem mass analysis and database research, it was revealed that the purified T. granosa anticoagulant protein (TGAP) has 7.7 kDa of molecular mass and its partial sequence, HTHLQRAPHPNALGYHGK, has a high identity (64%) with serine/threonine kinase derived from Rhodopirellula baltica (order Planctomycetales, marine bacteria). TGAP could potently prolong thrombin time (TT), corresponding to inhibition of thrombin (FIIa) formation. Specific factor inhibitory assay showed that TGAP inhibits FVa among the major components of prothrombinase complex. In vitro assay for direct-binding affinity using surface plasmon resonance (SPR) spectrometer indicated that TGAP could be directly bound with FVa. In addition, the binding affinity of FVa to FII was decreased by addition of TGAP in dose-dependant manner ($IC_{50}$ value = 77.9 nM). These results illustrated that TGAP might interact with a heavy chain of FVa ($FVa_H$) bound to FII in prothrombin complex. The present study elucidated that non-cytotoxic T. granosa anticoagulant protein (TGAP) bound to FVa can prolong blood coagulation time by inhibiting conversion of FII to FIIa in blood coagulation cascade. In addition, TGAP did not significantly (P < 0.05) show fibrinolytic activity and cytotoxicity on venous endothelial cell line (ECV 304).