• 한국미생물·생명공학회지
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• 제40권4호
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• pp.303-309
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• 2012

Keratinases are of particular interest because of their action on insoluble keratins and generally on a broad range of protein substrates. Alkalophilic and neutrophilic actinomycete strains isolated from different soil samples, rich in keratinaceous substances were screened for keratinolytic activity. An alkalophilic isolate, TBG-S13A5, was found to possess good keratinolytic activity and was able to utilize feather as the sole carbon and nitrogen source. TBG-S13A5 exhibited an off-white aerial mass color with a rectus-flexibilis type of spore chain. The morphological, microscopical and biochemical characters were comparable with that of Streptomyces albidoflavus. Fatty acid methyl ester profiling (FAME) and 16S rDNA sequence analysis confirmed its identity as a strain of S. albidoflavus. Under submerged fermentation conditions, maximum protease production was recorded on the $5^{th}$ day of incubation at $30^{\circ}C$, using basal broth of pH 9.0 with 0.25% (w/v) white chicken feather. This strain could affect feather degradation when the initial pH was 8 and above and maximum protease production was recorded when the initial pH was around 10.5. The effectiveness of the crude enzyme in destaining and leather dehairing were also demonstrated.

• Journal of Microbiology and Biotechnology
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• 제19권9호
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• pp.873-880
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• 2009

A novel xylanase gene, Kxyn, was cloned from Kocuria sp. Mn22, a bacteria isolated from the deep sea of the east Pacific. Kxyn consists of 1,170 bp and encodes a protein of 390 amino acids that shows the highest identity (63%) with a xylanase from Thermohifida fusca YX. The mature protein with a molecular mass of approximately 40 kDa was expressed in Escherichia coli BL21 (DE3). The recombinant Kxyn displayed its maximum activity at $55^{\circ}C$ and at pH 8.5. The $K_m,\;V_{max}$, and $k_{cat}$ values of Kxyn for birchwood xylan were 5.4 mg/ml, $272{\mu}mol/min{\cdot}mg$, and 185.1/s, respectively. Kxyn hydrolyzed birchwood xylan to produce xylobiose and xylotriose as the predominant products. The activity of Kxyn was not affected by $Ca^{2+},\;Mg^{2+},\;Na^+,\;K^+$, ${\beta}$-mercaptoethanol, DTT, or SDS, but was strongly inhibited by $Hg^{2+},\;Cu^{2+},Zn^{2+}$, and $Pb^{2+}$. It was stable over a wide pH range, retaining more than 80% activity after overnight incubation at pH 7.5-12. Kxyn is a cellulase-free xylanase. Therefore, these properties make it a candidate for various industrial applications.

• 한국콘텐츠학회논문지
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• 제15권11호
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• pp.104-114
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• 2015

서민이라는 용어는 언론이나 정치적 논의에서 빈번하게 사용되어 왔다. 그러나 서민이 누구인지, 서민을 어떤 기준으로 정의할 수 있는지, 서민과 중산층은 차이가 있는지에 대한 구체적인 논의가 부족하였다. 이 논문은 전국 규모의 설문조사와 25명을 대상으로 심층면접을 수행하여 서민에 대한 정의와 인식을 탐색적인 수준에서 살펴보았다. 대다수의 조사응답자들은 스스로를 서민으로 인식하며, 서민을 정의하는 가장 중요한 기준은 경제적 소득이라고 하였다. 구체적으로는 서민은 고등학교 졸업, 월평균 200~300만원의 수입, 20평 전셋집에 거주하는 영세자영업자나 숙련노동자, 낮은 직급의 화이트칼라의 직업을 가진 사람들로 정의하였다. 또한 서민은 계층상승의 가능성도 있지만 계층하락의 불안감도 지니는, 빈민과 중산층 사이에 끼어 있으면서 일부는 중첩된 집단으로 인식하였다. 스스로를 서민으로 규정하는 사람들은 중산층을 부러워하거나 자신의 계층적 위치를 폄하하기 보다는 자신의 계층적 지위에 대하여 자긍심을 가지면서 미래에 대한 희망을 지니는 것으로 나타났다. 그러나 서민 스스로의 자긍심과 희망이 유지, 실현되기 위해서는 다양한 사회적 지원이 필요한 것으로 보인다. 서민을 정의하고 서민의 구체적인 특성과 정책욕구를 파악하는 보다 심층적인 분석과 연구가 필요하다.

• 한국수산과학회지
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• 제50권3호
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• pp.302-310
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• 2017

Interferon regulatory factor 8 (IRF8) is essential for the development of B and T cells, as well as for the activity of dendritic cells and macrophages. We performed molecular characterization of IRF8 from rock fish, Sebastes schlegelii (Ss), and investigated the spatial and temporal profile of mRNA expression after challenge with lipopolysaccharide (LPS), polyinosinic:polycytidylic acid (poly I:C), or Streptococcus iniae. The full-length cDNA sequence of SsIRF8 was 1,657 bp, containing an ORF of 1,266 bp. The gene had a predicted molecular mass of 47.7 kDa and an isoelectric point of 5.99. The amino acid sequence coded by this gene showed the highest degree of identity (90.8%) and similarity (96.2%) with IRF8 from Oplegnathus fasciatus. The SsIRF8 mRNA was expressed ubiquitously, at varying levels, with the highest level of expression observed in the spleen. To confirm the role of SsIRF8 in mediating the immune response, we measured SsIRF8 mRNA expression in the splenic tissue at different time points after injection with LPS, poly I:C, or S. iniae. The qRT-PCR results showed that SsIRF8 mRNA expression in the poly I:C-injected group was highly upregulated 6 hr after exposure (P<0.05). Expression of SsIRF8 mRNA in the S. iniae-injected group peaked at 24 hr. These results suggest that SsIRF8 might be important in regulating the strength of the rockfish immune response to immunostimulatory agents.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2004년도 The 3rd Annual Conference for The Korean Society for Bioinformatics Association of Asian Societies for Bioinformatics 2004 Symposium
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• pp.265-271
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• 2004

The interaction network of protein -protein plays an important role to understand the various biological functions of cells. Currently, the high -throughput experimental techniques (two -dimensional gel electrophoresis, mass spectroscopy, yeast two -hybrid assay) provide us with the vast amount of data for protein-protein interaction at the proteome scale. In order to recognize the role of each protein in their network, the efficient bioinformatical and computational analysis methods are required. We propose a systematic and mathematical method which can analyze the protein -protein interaction network rigorously and enable us to capture the biological and physical essence of a topological character and stability of protein -protein network, and sensitivity of each protein along the biological pathway of their network. We set up a Laplacian matrix of spectral graph theory based on the protein-protein network of yeast proteome, and perform an eigenvalue analysis and apply a perturbation method on a Laplacian matrix, which result in recognizing the center of protein cluster, the identity of hub proteins around it and their relative sensitivities. Identifying the topology of protein -protein network via a Laplacian matrix, we can recognize the important relation between the biological pathway of yeast proteome and the formalism of master equation. The results of our systematic and mathematical analysis agree well with the experimental findings of yeast proteome. The biological function and meaning of each protein cluster can be explained easily. Our rigorous analysis method is robust for understanding various kinds of networks whether they are biological, social, economical...etc

• 대한의생명과학회지
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• 제12권4호
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• pp.419-425
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• 2006

Phospholipase $C-{\gamma}1\;(PLC-{\gamma}1)$ is an important signaling molecule for cell proliferation and differentiation. $PLC-{\gamma}1$ contains two pleckstrin homology (PH) domains, which are responsible for protein-protein interaction and protein-lipid interaction. $PLC-{\gamma}1$ also has two Src homology (SH)2 domains and a SH3 domain, which are responsible for protein- protein interaction. To identity proteins that specifically binds to PH domain of $PLC-{\gamma}1$, we prepared and incubated the glutathione S-transferase(GST)-fused PH domains of $PLC-{\gamma}1$ with COS7 cell lysate. We found that 90 kDa protein specifically binds to PH domain of $PLC-{\gamma}1$. By matrix-assisted laser desorption ionization time of flight-mass spectrometry, the 90 kDa protein revealed to be heat shock protein (Hsp) $90{\beta}$. Hsp $90{\beta}$ is a molecular chaperone that stabilizes and facilitates the folding of proteins that are involved in cell signaling, including receptors for steroids hormones and a variety of protein kinases. To know whether Hsp $90{\beta}$ affects on $PLC-{\gamma}1$ activity, we performed $PIP_2$ hydrolyzing activity of $PLC-{\gamma}1$ in the presence of purified Hsp $90{\beta}$ in vitro. Our results show that the Hsp $90{\beta}$ dose-dependently inhibits the enzymatic activity of $PLC-{\gamma}1$ and further suggest that Hsp $90{\beta}$ regulates cell growth and differentiation via regulation of $PLC-{\gamma}1$ activity.

• 복식
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• 제54권7호
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• pp.135-150
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• 2004

The purpose of this research was intended to investigate drag queens' and drag kings' gender identity not only as a comic and desexualized drag borrowing external characteristics of the opposite sex but also as the subject visualizing and performing' the third sex'. It also aimed to examine formative aesthetic characteristics and aesthetical value of drag queen's and drag king's fashion and to confirm the functions of drag queen's and drag kins's fashion to establish, visualize and Perform the discordant sex. For this process, research steps were as follows : 1. Understanding drag, drag queen and drag king in social, psychological context as well as in gender context. 2. Finding visualized forms which drag performance interchange with mass media 3. Analyzing similarities and differences between drag queen's fashion and drag king's fashion. 4. Examining the aesthetical characteristics and the value of drag queen's and drag king's fashion. The results of characteristics of drag queen's fashion could be categorized into stereotype, mimicry, kitch, inconsistency and commercialism. And the characteristics of drag king's fashion could be divided into reality, self-consciousness, mimicry, inconsistency, subversion and multiplicity. Drag queens create plausible impressions of feminity through the use of wigs, dresses, jewelry, makeup, hormones and through &role Playing&. Similarly drag kings produce a plausible masculinity taking gay male aesthetic using suits, crotch stuffers, facial hair, and greased hair. Male and female impersonation produce very different notions of gender performance for male and female embodiment. Drag kings' performance of masculinity demands authentic property of bodies so rather nonperformative, while drag queens' performance of femininity depends on more visible and theatrical fashion.

• 대한기계학회논문집B
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• 제35권4호
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• pp.353-360
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• 2011

액적 충돌은 물방울 형성 및 분무 유동 등의 현상을 예측하는데 있어 매우 중요하다. 이러한 액적 충돌은 액적 속도, 충돌 파라미터, 액적 크기비에 영향을 받아, 충돌 후 거동 특성이 결정된다. 충돌 후 액적은 반사, 합일, 스트레칭 분리, 리플렉시브 분리와 같은 거동 특성을 갖는다. 본 연구에서는 레벨셋 방법을 사용하여 충돌 후 액적 거동 특성에 대한 이상유동 해석을 수행하였다. 정면충돌 현상에 대한 2차원 축대칭 해석으로부터 합일 및 리플렉시브 분리 현상을, 비중심충돌 현상에 대한 3차원 해석으로부터 합일, 리플렉시브 분리, 스트레칭 분리 현상을 예측할 수 있었다. 이러한 해석 결과는 기존 실험 및 이론적 연구 결과와 일치하는 결과를 보였다. 또한, 초기 액적의 부피비에 대한 수송 방정식을 사용하여 충돌하는 두 액적의 성분을 추적하였다. 이로부터 크기가 다른 두 액적의 정면충돌에 대한 액적 성분 추적을 통해 액적 거동 및 액적 성분에 대해 분석하였다.

• Toxicological Research
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• 제11권2호
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• pp.295-302
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• 1995

Menadione-ghitathione conjugate (MEN-SG), a metabolite of menadione, is known to be a redoxcycler in rat hepatocyte subcellular fraction. Therefore, it was assumed that MEN-SG could exert cytotoxlclty to ral platelets, another target tissue of menadione. We first synthesized MEN-SG, the identity of which was verified by mass, $^1{H}$-NMR and UV-visible spectra. In addition, the stability of MEN-SG was investigated in biological assay system. MEN-SG was degraded in a time-dependent manner in DMSO which had been used as a vehicle and thus, tris-HCl buffer was used as a vehicle of MEN-SG despite the low solubility in it. Perchloric acid as well as platelets itself did not affect the stability of MEN-SG. Our next attempt was the evaluation of cytotoxicity of MEN-SG in rat platelets. MEN-SG did not induce cytotoxicity to rat platelets measured by two different methods, LDH release and turbidity changes. The extents of oxygen consumption by MEN-SG in intact platelets were significantly lower than those by menadione, though it had been observed that oxygen consumptions by menadione and MENSG were similar in subcellular fractioas of platelets. These results suggest that MEN-SG is not toxic to rat platelets despite its redox cycling capacity and glutathione conjugation reaction of menadione could be regarded as a detoxification process.

• Journal of Ginseng Research
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• 제42권1호
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• pp.21-26
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• 2018

Background: Furosine (${\varepsilon}$-N-2-furoylmethyl-L-lysine, FML) is an amino acid derivative, which is considered to be an important indicator of the extent of damage (deteriorating the quality of amino acid and proteins due to a blockage of lysine and a decrease in the digestibility of proteins) during the early stages of the Maillard reaction. In addition, FML has been proven to be harmful because it is closely related to a variety of diseases such as diabetes. The qualitative analysis of FML in fresh and processed ginsengs was confirmed using HPLC-MS. Methods: An ion-pair reversed-phase LC method was used for the quantitative analysis of FML in various ginseng samples. Results: The contents of FML in the ginseng samples were 3.35-42.28 g/kg protein. The lowest value was observed in the freshly collected ginseng samples, and the highest value was found in the black ginseng concentrate. Heat treatment and honey addition significantly increased the FML content from 3.35 g/kg protein to 42.28 g/kg protein. Conclusion: These results indicate that FML is a promising indicator to estimate the heat treatment degree and honey addition level during the manufacture of ginseng products. The FML content is also an important parameter to identity the quality of ginseng products. In addition, the generation and regulation of potentially harmful Maillard reaction products-FML in ginseng processing was also investigated, providing a solid theoretical foundation and valuable reference for safe ginseng processing.