• Title/Summary/Keyword: Marker expansion

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Improvement of the Discrimination Capacity through the Expansion of Y Chromosomal STR Markers

  • Dong Gyu Lee;So Eun Lee;Ji Hwan Park;Si-Keun Lim;Ju Yeon Jung
    • Biomedical Science Letters
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    • v.29 no.4
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    • pp.302-313
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    • 2023
  • Y chromosomal short tandem repeat (Y-STR) markers have been developed continuously to complement forensic DNA analyses and population genetic studies. Initially, we collected data from previously reported Korean population Y-STR haplotype studies on 1133 individuals. We then conducted a marker expansion analysis using a dataset from the Y-STR Haplotype Reference Database (YHRD), covering up to 29 Y-STRs, referred to as Ymax. Additionally, we examined the impact of rapidly mutating (RM) Y-STRs included in this expanded marker set on the discrimination capacity. We observed that marker expansions both with (0.9896), and without (0.9510), RM Y-STR improved the discrimination capacity. Subsequently, we focused on 16 individuals belonging to seven distinct groups sharing identical haplotypes. These particular haplotypes had been previously identified among 476 unrelated males using 23 Y-STR markers from the PowerPlex® Y23 System. We expanded the marker panel up to Ymax to explore how discrimination improved with an expansion of Y-STR markers for these 16 individuals. Among the expanded markers, DYS627, which had high discriminatory power, had a high mutation rate (1.10 × 10-2) and high gene diversity (0.83). In contrast, DYF387S1 displayed high gene diversity (0.95) but a relatively low mutation rate (2.80 × 10-3). We propose that these findings will be valuable in the selection of suitable Y-STR markers, depending on the objectives of forensic analyses. Additionally, the presence of frequently observed Y-haplotypes in Korean population will facilitate statistical interpretation in Y-STR DNA profiling.

Marker-less Calibration of Multiple Kinect Devices for 3D Environment Reconstruction (3차원 환경 복원을 위한 다중 키넥트의 마커리스 캘리브레이션)

  • Lee, Suwon
    • Journal of Korea Multimedia Society
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    • v.22 no.10
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    • pp.1142-1148
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    • 2019
  • Reconstruction of the three-dimensional (3D) environment is a key aspect of augmented reality and augmented virtuality, which utilize and incorporate a user's surroundings. Such reconstruction can be easily realized by employing a Kinect device. However, multiple Kinect devices are required for enhancing the reconstruction density and for spatial expansion. While employing multiple Kinect devices, they must be calibrated with respect to each other in advance, and a marker is often used for this purpose. However, a marker needs to be placed at each calibration, and the result of marker detection significantly affects the calibration accuracy. Therefore, a user-friendly, efficient, accurate, and marker-less method for calibrating multiple Kinect devices is proposed in this study. The proposed method includes a joint tracking algorithm for approximate calibration, and the obtained result is further refined by applying the iterative closest point algorithm. Experimental results indicate that the proposed method is a convenient alternative to conventional marker-based methods for calibrating multiple Kinect devices. Hence, the proposed method can be incorporated in various applications of augmented reality and augmented virtuality that require 3D environment reconstruction by employing multiple Kinect devices.

The Effect of the Position Change of the Magnification Marker and the Stitching Range on the Image in the Examination of the Femur Radiography Using Stitching Technique (스티칭 기법을 이용한 대퇴골 방사선검사 시 확대 표식자 위치 및 스티칭 범위 변화가 영상에 미치는 영향)

  • Hong, Seong-Woo;Joo, Young-Cheol;Kim, Sheung-Hyuk;Go, Ye-Weon;Kim, Jong-Suk
    • Journal of radiological science and technology
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    • v.43 no.5
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    • pp.331-336
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    • 2020
  • In this study, we would like to examine the effect of the location of the magnification marker and the change in the stitching range on the magnification marker and the expansion of the anatomical structure in the examination of femoral stitching radiation using the stitching technique, and to find out the usability of the femoral stitching radiation examination in the preoperative examination for THRA. The measured values measured by 5 researchers were summed and presented through descriptive statistics. When the position of the magnification marker was changed, the average value of the measured value was in the range of 113.54 to 113.90 mm. The anatomical structures of the femur were measured in the range of 57.24 to 57.78 mm, 27.20 to 27.56 mm, and 70.11 to 70.81 mm, respectively. This showed a similar deviation from the expansion rate of previous related papers using markers. Therefore, it is considered that femoral stitching X-ray examination using magnification markers can be used for preoperative examination for THRA.

Discriminability of Molecular Markers Based on Muclear Ribosomal ITS Sequences of Fagopyrum esculentum and F. tataricum (단메밀과 쓴메밀의 ITS 염기서열 기반 식별마커)

  • Oh, Dae-Ju;Hyun, Ho-Bong;Lim, Tae-Joon;Yoon, Seon-A;Ham, Young-Min;Yoon, Weon-Jong;Yang, Woo-Sam;Jung, Yong-Hwan
    • Korean Journal of Organic Agriculture
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    • v.26 no.4
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    • pp.745-757
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    • 2018
  • We analyzed the nuclear ribosomal internal transcribed spacer (ITS) sequence of common buckwheat, Fagopyrum esculentum and tartary buckwheat, F. tataricum. The diversity of the nucleotides and haplotypes, Tajima's D, and Fu's Fs was analyzed and compared among the varieties of common buckwheat and tartary buckwheat. The diversity of nucleotides and haplotypes indicated that the buckwheat populations had undergone rapid population expansion but D and Fs did not support their expansion statistically. The phylogenetic analysis of ITS sequences did not clearly establish the phylogenetic relationships between the varieties of common buckwheat. The In/Del sequence of ITS-1 region could, therefore, be used as a DNA marker to distinguish raw or manufactured products derived from common buckwheat and tartary buckwheat.

Studies on the Metabolic Cooperativity between Ooccte and Cumulus Cells in Mammalian Oocyte Cumulus Complexes in vitro (포유동물 난자-난구 복합체의 Metabolic cooperativity)

  • 고선근;나철호;권혁방
    • The Korean Journal of Zoology
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    • v.31 no.2
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    • pp.81-86
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    • 1988
  • The relationship between cumulus cell expansion, cocyte maturation and metabolic cooperativitiy was investigated by using mouse and pig cocyte-cumulus complexes in vitro. Cocyte germinal vesicle breakdown (GVBD) and cumulus expansion were manipulated with hormones or reagents which increase intracellular cAMP leveL Metabolic cooperativity between oocyte and cumulus cells was assessed by determination of the fraction of radiolabelled uridine marker that was transferred from the cumulus mass to the oocyte. Uptake of uddine marker by mouse and pig cumulus mass was increased by about fourfold of basal level with the stimulation of hormones (human choriononic gonadotrophin, HCG; follicle stimulating hormone, FSH) or cyclic AMP sttmulators (3-isobutyl-1-methylxanthine, IBMX; forskolin) during culture. However, the fraction of uridine that was transferred from the cumulus mass to the cocyte (transfer ratio) was gradually decreased during culture, irrespective with the presence of hormones or stimulators. The decrease of the transfer ratio was not correlated with the state of occyte whether they have GV or not, or with the degree of cumulus expansion. In mouse complexes, HCG induced more significant reducton of transfer ratio than other treatments. These results do not support the idea that modulations of metabolic cooperativity between cumulus cells and oocytes are important for the regulation of meiotic resumption in mammals.

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A study on the Image Augmented Reality Card using Augmented Reality (증강현실을 이용한 이미지기반 AR카드 활용방안 연구)

  • Park, Ki-Deok;Chung, Jean-Hun
    • Journal of Digital Convergence
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    • v.12 no.8
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    • pp.467-474
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    • 2014
  • Augmented reality in the smart environments is the interaction between the user and the media through the expansion of a variety of experiences is provided. How to use the location information, how to use markers, utilizing real-time marker-less interaction with the real world and the virtual world, such as the mapping method intermediary situated in real-time. Expansion of the elements of experience, and augmented reality as a tool of information being utilized. Educational Edutainment AR card to take advantage in terms of content was a real-time marker-less approach to create an image map image map was constructed urbanization. Stereoscopic 3D environment, taking advantage of the object produced by combining augmented reality and way of interaction, educational content. Help in terms of active and passive involvement pra realistic interest leads to a way of giving tangible.

A case of canine maxillary gingival acanthomatous epulis

  • Kim Bum-Seok;Park Hee-Jin;Cho Jae-Woo;Camer Gerry Amor;Berzina Dace;Chekarova Irina;Ejaz Sohail;Cheon Seung-Ki;Kwon Jung-Kee;Lim Chae-Woong
    • Korean Journal of Veterinary Service
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    • v.29 no.4
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    • pp.507-511
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    • 2006
  • Acanthomatous epulis is a malignant form of gingival tumor. This is a case of epulis affecting a four-year old, intact, female Maltese dog raised as indoor pet in Iksan city. Clinical examination showed an enlarged palpable mass along the rostral maxillary gingiva. Section of the excised mass showed epithelial overgrowth and expansion from the basal portions through the tissue stroma. Post-surgical radio-graph showed displacement of the first and second maxillary incisors. Histochemical staining showed strongly positive reaction to epithelial marker cytokeratin and moderately positive reaction to connective tissue marker vimentin. This case was diagnosed as a form of maxillary gingival acanthomatous epulis. Routine clinical oral examination is suggested.

Dynamic Expansion of Semantic Dictionary for Topic Extraction in Automatic Summarization (자동요약의 주제어 추출을 위한 의미사전의 동적 확장)

  • Choo, Kyo-Nam;Woo, Yo-Seob
    • Journal of IKEEE
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    • v.13 no.2
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    • pp.241-247
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    • 2009
  • This paper suggests the expansion methods of semantic dictionary, taking Korean semantic features account. These methods will be used to extract a practical topic word in the automatic summarization. The first is the method which is constructed the synonym dictionary for improving the performance of semantic-marker analysis. The second is the method which is extracted the probabilistic information from the subcategorization dictionary for resolving the syntactic and semantic ambiguity. The third is the method which is predicted the subcategorization patterns of the unregistered predicate, for the resolution of an affix-derived predicate.

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A Review of Extended STR Loci and DNA Database

  • Cho, Yoonjung;Lee, Min Ho;Kim, Su Jin;Park, Ji Hwan;Jung, Ju Yeon
    • Biomedical Science Letters
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    • v.28 no.3
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    • pp.157-169
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    • 2022
  • DNA typing is the typical technology in the forensic science and plays a significant role in the personal identification of victims and suspects. Short tandem repeat (STR) is the short tandemly repeated DNA sequence consisting of 2~7 bp DNA units in specific loci. It is disseminated across the human genome and represents polymorphism among individuals. Because polymorphism is a key feature of the application of DNA typing STR analysis, STR analysis becomes the standard technology in forensics. Therefore, the DNA database (DNA-DB) was first introduced with 4 essential STR markers for the application of forensic science; however, the number of STR markers was expanded from 4 to 13 and 13 to 20 later to counteract the continuously increased DNA profile and other needed situations. After applying expanded STR markers to the South Korean DNA-DB system, it positively affected to low copy number analysis that had a high possibility of partial DNA profiles, and especially contributed to the theft cases due to the high portion of touch DNA evidence in the theft case. Furthermore, STR marker expansion not only contributed to the resolution of cold cases but also increased kinship index indicating the potential for improved kinship test accuracy using extended STR markers. Collectively, the expansion of the STR locus was considered to be necessary to keep pace with the continuously increasing DNA profile, and to improve the data integrity of the DNA-DB.

The effects of blastocyst morphological score and blastocoele re-expansion speed after warming on pregnancy outcomes

  • Yin, Huiqun;Jiang, Hong;He, Ruibing;Wang, Cunli;Zhu, Jie;Li, Yang
    • Clinical and Experimental Reproductive Medicine
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    • v.43 no.1
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    • pp.31-37
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    • 2016
  • Objective: The aim of this study was to investigate associations between the morphology score of blastocysts and blastocoele re-expansion speed after warming with clinical outcomes, which could assist in making correct and cost-effective decisions regarding the appropriate time to vitrify blastocysts and to transfer vitrified-warmed blastocysts. Methods: A total of 327 vitrified-warmed two-blastocyst transfer cycles in women 38 years old and younger were included in this retrospective study. Results: The clinical pregnancy rate (CPR) and implantation rate (IR) of transfers of two good-morphology grade 4 blastocysts vitrified on day 5 (64.1% and 46.8%, respectively) were significantly higher than the CPR and IR associated with the transfers of two good-morphology grade 3 blastocysts vitrified on day 5 (46.7% and 32.2%, respectively). No significant differences were found in the CPR and IR among the transfers of two good-morphology grade 4 blastocysts regardless of the day of cryopreservation. Logistic regression analysis showed that blastocoele reexpansion speed after warming was associated with the CPR. Conclusion: The selection of a good-morphology grade 4 blastocyst to be vitrified could be superior to the choice of a grade 3 blastocyst. Extending the culture of grade 3 blastocysts and freezing grade 4 or higher blastocysts on day 6 could lead to a greater likelihood of pregnancy. Since re-expansion was shown to be a morphological marker of superior blastocyst viability, blastocysts that quickly re-expand after warming should be prioritized for transfer.