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http://dx.doi.org/10.11625/KJOA.2018.26.4.745

Discriminability of Molecular Markers Based on Muclear Ribosomal ITS Sequences of Fagopyrum esculentum and F. tataricum  

Oh, Dae-Ju ((재)제주테크노파크 생물종다양성연구소)
Hyun, Ho-Bong ((재)제주테크노파크 생물종다양성연구소)
Lim, Tae-Joon ((재)제주테크노파크 생물종다양성연구소)
Yoon, Seon-A ((재)제주테크노파크 생물종다양성연구소)
Ham, Young-Min ((재)제주테크노파크 생물종다양성연구소)
Yoon, Weon-Jong ((재)제주테크노파크 생물종다양성연구소)
Yang, Woo-Sam (제주특별자치도 농업기술원)
Jung, Yong-Hwan ((재)제주테크노파크 생물종다양성연구소)
Publication Information
Korean Journal of Organic Agriculture / v.26, no.4, 2018 , pp. 745-757 More about this Journal
Abstract
We analyzed the nuclear ribosomal internal transcribed spacer (ITS) sequence of common buckwheat, Fagopyrum esculentum and tartary buckwheat, F. tataricum. The diversity of the nucleotides and haplotypes, Tajima's D, and Fu's Fs was analyzed and compared among the varieties of common buckwheat and tartary buckwheat. The diversity of nucleotides and haplotypes indicated that the buckwheat populations had undergone rapid population expansion but D and Fs did not support their expansion statistically. The phylogenetic analysis of ITS sequences did not clearly establish the phylogenetic relationships between the varieties of common buckwheat. The In/Del sequence of ITS-1 region could, therefore, be used as a DNA marker to distinguish raw or manufactured products derived from common buckwheat and tartary buckwheat.
Keywords
common buckwheat; In/Del marker; Internal Transcribed Spacer (ITS); tartary buckwheat;
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