• Title/Summary/Keyword: Marker Conversion

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Discovery of Performance Traits-Linked Microsatellite Markers in Channel Catfish (Ictalurus punctatus)

  • Kim, Soon-Hag
    • Journal of Aquaculture
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    • v.18 no.2
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    • pp.130-132
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    • 2005
  • Genomics research has two ultimate applied goals: to Isolate and clone genes of economic importance for bio-technology and gene-assisted selection (GAS), and to locate and use markers for marker-assisted selection (MAS) in selective breeding programs. To this end, we have identified linked markers for feed conversion efficiency growth rate, and disease resistance to enteric septicemia of catfish (ESC). Three microsatellite markers Ip266, Ip384, and Ip607 were identified to be linked to feed conversion efficiency. Similarly one marker each was identified to be linked to growth rate (Ip607) and disease resistance to ESC (Ip477). Ip607 marker linked to both growth rate and feed conversion efficiency, indicating that the QTL for both growth rate and feed conversion efficiency may either be the same or located in the same chromosomal region in the catfish genome. On phenotypic evaluation, certain traits such as growth rate can be accurately evaluated by body weight evaluation while other traits such as disease resistance can be quite complex. The linked DNA markers will be highly useful for MAS programs and for directing further efforts of genomic mapping for important quantitative traits.

Improved Tooth Detection Method for using Morphological Characteristic (형태학적 특징을 이용한 향상된 치아 검출 방법)

  • Na, Sung Dae;Lee, Gihyoun;Lee, Jyung Hyun;Kim, Myoung Nam
    • Journal of Korea Multimedia Society
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    • v.17 no.10
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    • pp.1171-1181
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    • 2014
  • In this paper, we propose improved methods which are image conversion and extraction method of watershed seed using morphological characteristic of teeth on complement image. Conventional tooth segmentation methods are occurred low detection ratio at molar region and over, overlap segmentation owing to specular reflection and morphological feature of molars. Therefore, in order to solve the problems of the conventional methods, we propose the image conversion method and improved extraction method of watershed seed. First, the image conversion method is performed using RGB, HSI space of tooth image for to extract boundary and seed of watershed efficiently. Second, watershed seed is reconstructed using morphological characteristic of teeth. Last, individual tooth segmentation is performed using proposed seed of watershed by watershed algorithm. Therefore, as a result of comparison with marker controlled watershed algorithm and the proposed method, we confirmed higher detection ratio and accuracy than marker controlled watershed algorithm.

Effect of (-)-epigallocatechin-3-gallate on lipogenesis in 3T3-L1 Cell

  • Kang, Shin-Seok;Park, Jae-Myung;Choi, Hae-Yeon;Cho, Woo-Young;Lee, Jong-In
    • Korean Journal of Veterinary Service
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    • v.26 no.4
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    • pp.339-343
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    • 2003
  • We studied the effect of epigallocatechin-3-gallate(EGCG) on the adipose conversion of 3T3-L1 cells by insulin. In the 10 days of culture with insulin, the fat cells exhibited the increased and larger intracytoplasmic lipid droplets. In contrast, the levels of triglyceride(TG), a marker of adipose conversion, were decreased. However, the levels of glucose were decreased in the adipose conversion. In addition, levels of cholesterol were decreased in the differentiated 3T3-L1 cells.

The overall structure and operation of the IJG JPEG compressor (IJG JPEG 부호기의 구조와 작동)

  • 채희중;이호석
    • Proceedings of the Korean Information Science Society Conference
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    • 1999.10b
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    • pp.262-264
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    • 1999
  • 본 논문은 IJG(Independent JPEG Group)의 JPEG 부호기의 처리 과정과 동작을 기술한다. IJG JPEG 부호기의 구조는 color conversion, downsampling, preprocessing 과정과 MCU 처리, FDCT, quantization, entropy encoding(sequential 혹은 progressive, huffman 혹은 arithmetic)의 실제적인 JPEG 압축 과정인 JPEG proper로 구성된다. 또한 이러한 모듈들외에 시스템 전체 controller, marker 생성기, 기억장소 관리, 에러 처리를 위한 모듈들을 포함하고 있다. 이에 본 논문에서는 IJG JPEG 부호기의 전체 시스템 구조 및 controller 와 주요 모듈간 인터페이스, 시스템에서 사용하는 주요 자료 구조에 대하여 분석하고자 한다.

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Development of a Sequence Characteristic Amplified Region Marker linked to the L4 Locus Conferring Broad Spectrum Resistance to Tobamoviruses in Pepper Plants

  • Kim, Hyun Jung;Han, Jung-Heon;Yoo, Jae Hyoung;Cho, Hwa Jin;Kim, Byung-Dong
    • Molecules and Cells
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    • v.25 no.2
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    • pp.205-210
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    • 2008
  • To develop molecular markers linked to the $L^4$ locus conferring resistance to tobamovirus pathotypes in pepper plants, we performed AFLP with 512 primer combinations for susceptible (S pool) and resistant (R pool) DNA bulks against pathotype 1.2 of pepper mild mottle virus. Each bulk was made by pooling the DNA of five homozygous individuals from a T10 population, which was a near-isogenic $BC_4F_2$ generation for the $L^4$ locus. A total of 19 primer pairs produced scorable bands in the R pool. Further screening with these primer pairs was done on DNA bulks from T102, a $BC_{10}F_2$ derived from T10 by back crossing. Three AFLP markers were finally selected and designated L4-a, L4-b and L4-c. L4-a and L4-c each underwent one recombination event, whereas no recombination for L4-b was seen in 20 individuals of each DNA bulk. Linkage analysis of these markers in 112 $F_2$ T102 individuals showed that they were each within 2.5 cM of the $L^4$ locus. L4-b was successfully converted into a simple 340-bp SCAR marker, designated L4SC340, which mapped 1.8 cM from the $L^4$ locus in T102 and 0.9 cM in another $BC_{10}F_2$ population, T101. We believe that this newly characterized marker will improve selection of tobamovirus resistance in pepper plants by reducing breeding cost and time.

Numerical Simulation of Fully Nonlinear Free-Surface Flow around Seawall with Slope (경사면을 갖는 월파형 구조물 주위의 비선형성 자유표면류의 수치 시뮬레이션)

  • Park, Jong-Chun;Park, Dong-In;Lee, Sang-Beom;Hong, Gi-Yong;Sun, Sung-Bu
    • Journal of Ocean Engineering and Technology
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    • v.19 no.3
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    • pp.18-24
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    • 2005
  • Wave overtopping is one of the most important processes for the design of seawalls. The term "wave overtopping" is used here to refer to the processes where waves hit a sloping structure run up the slope and, if the crest level of the slope is lower than the highest run up level, overtop the structure. Wave overtopping is dependent on the processes associated with breaking wave. A numerical model based on Navier-Stokes equation and the Marker-density function for predicting wave overtopping of coastal structures is developed in this paper. In order to evaluate the present model, two simulations are tested. One is overflow without waves at vertical seawall, and the other is wave overtopping at sloping seawalls.

Involvement of ROS in Curcumin-induced Autophagic Cell Death

  • Lee, Youn-Ju;Kim, Nam-Yi;Suh, Young-Ah;Lee, Chu-Hee
    • The Korean Journal of Physiology and Pharmacology
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    • v.15 no.1
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    • pp.1-7
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    • 2011
  • Many anticancer agents as well as ionizing radiation have been shown to induce autophagy which is originally described as a protein recycling process and recently reported to play a crucial role in various disorders. In HCT116 human colon cancer cells, we found that curcumin, a polyphenolic phytochemical extracted from the plant Curcuma longa, markedly induced the conversion of microtubule-associated protein 1 light chain 3 (LC3)-I to LC3-II and degradation of sequestome-1 (SQSTM1) which is a marker of autophagosome degradation. Moreover, we found that curcumin caused GFP-LC3 formation puncta, a marker of autophagosome, and decrease of GFP-LC3 and SQSTM1 protein level in GFP-LC3 expressing HCT116 cells. It was further confirmed that treatment of cells with hydrogen peroxide induced increase of LC3 conversion and decrease of GFP-LC3 and SQSTM1 levels, but these changes by curcumin were almost completely blocked in the presence of antioxidant, N-acetylcystein (NAC), indicating that curcumin leads to reactive oxygen species (ROS) production, which results in autophagosome development and autolysosomal degradation. In parallel with NAC, SQSTM1 degradation was also diminished by bafilomycin A, a potent inhibitor of autophagosome-lysosome fusion, and cell viability assay was further confirmed that cucurmin-induced cell death was partially blocked by bafilomycin A as well as NAC. We also observed that NAC abolished curcumin-induced activation of extracelluar signal-regulated kinases (ERK) 112 and p38 mitogen-activated protein kinases (MAPK), but not Jun N-terminal kinase (JNK). However, the activation of ERK1/2 and p38 MAPK seemed to have no effect on the curcumin-induced autophagy, since both the conversion of LC3 protein and SQSTM1 degradation by curcumin was not changed in the presence of NAC. Taken together, our data suggest that curcumin induced ROS production, which resulted in autophagic activation and concomitant cell death in HCT116 human colon cancer cell. However, ROS-dependent activation of ERK1/2 and p38 MAPK, but not JNK, might not be involved in the curcumin-induced autophagy.

Design and Implementation of Frontal-View Algorithm for Smartphone Gyroscopes (스마트폰 자이로센서를 이용한 Frontal-View 변환 알고리즘 설계 및 구현)

  • Cho, Dae-Kyun;Park, Seok-Cheon
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.12 no.6
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    • pp.199-206
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    • 2012
  • Attempt to use as a marker of natural objects directly in the real world, but there is a way to use the accelerometer of the smartphone, to convert the Frontal-View virtual, because it asks only the pitch of the camera, from the side there is a drawback that can not be converted to images. The proposed algorithm, to obtain the rotation matrix of axis 3 pitch, roll, yaw, we set the reference point of the yaw of the target image. Then, to compensate for the rotation matrix to determine Myon'inji any floor, wall, the ceiling of the target image. Finally, to obtain the homography matrix for obtaining the Frontal-View to account for the difference between the gyro sensor coordinate system and image coordinate system, so we can get the Frontal-View from the captured images through the projection transformation was designed. Was tested to convert Frontal-View the picture was taken in an environment smartphone environment surrounding floor, walls and ceiling in order to evaluate the conversion program Frontal-View has been implemented, in this paper, design and The conversion algorithm implementation, it was confirmed that to convert a regular basis Frontal-View footage taken from multiple angles.

Measurement of Fractional Exhaled Nitric Oxide in Adults: Comparison of Two Different Analyzers (NIOX VERO and NObreath)

  • Kang, Sung-Yoon;Lee, Sang Min;Lee, Sang Pyo
    • Tuberculosis and Respiratory Diseases
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    • v.84 no.3
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    • pp.182-187
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    • 2021
  • Background: Fractional exhaled nitric oxide (FeNO) is a non-invasive marker for eosinophilic airway inflammation and a good predictor of response to corticosteroids. There is a need for a reliable and accurate measurement method, as FeNO measurements have been widely used in clinical practice. Our study aimed to compare two FeNO analyzers and derive a conversion equation for FeNO measurements in adults. Methods: We included 99 participants who had chief complaints of chronic cough and difficulty in breathing. The participants underwent concurrent FeNO measurement using NIOX VERO (Circassia AB) and NObreath (Bedfont). We compared the values of the two devices and analyzed their correlation and agreement. We then formulated an equation to convert FeNO values measured by NObreath into those obtained by NIOX VERO. Results: The mean age of the participants was 51.2±17.1 years, with a female predominance (58.6%). Approximately 60% of the participants had asthma. The FeNO level measured by NIOX VERO (median, 27; interquartile range [IQR], 15-45) was significantly lower than that measured by NObreath (median, 38; IQR, 22-58; p<0.001). There was a strong positive correlation between the two devices (r=0.779, p<0.001). Additionally, Bland-Altman plots and intraclass correlation coefficient demonstrated a good agreement. Using linear regression, we derived the following conversion equation: natural log (Ln) (NObreath)=0.728×Ln (NIOX VERO)+1.244. Conclusion: The FeNO values of NIOX VERO and NObreath were in good agreement and had positive correlations. Our proposed conversion equation could help assess the accuracy of the two analyzers.