• Title/Summary/Keyword: Marine resources

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Purification of Myomodulin A and Myomodulin E from the Central Nervous System of the Sea Hare, Aplysia kurodai (군소 (Aplysia kurodai)의 중추신경계로부터 myomodulin A와 E의 정제)

  • KIM Chan-Hee;SEO Hae Jeom;HWANG Eun-Young;KIM Eun Jung;GO Hye-Jin;KIM In Hye;Seo Jung-Kil;Moon Jung-Hye;HUH Min-Do;PARK Nam Gyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.3
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    • pp.279-284
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    • 2001
  • This paper reports the purification of myomodulin A (MMA) and myomodulin E (MME) from the sea hare. The central nervous systems of 500 sea hare were extracted in an acidified solvent, after which four HPLC column systems were used to obtain pure peptides, The phasic contraction bioassay using a Mytilus edulis anterior byssus retractor muscle (ABRW) was applied to monitor all collected fractions. The pure peptides were submitted to Edman degradation based automated microsequencing. Mass spectrometry and chemical synthesis confirmed the sequence. The primary structures of MMA and MME were Pro-Met-Ser-Met-Leu-Arg-Leu-$NH_2$, (847.41 Da) and Cly-Leu-Gln-Met-Leu-Arg-Leu-$NH_2$, (830.50 Da), respectively. Synthetic peptides showed a modulating activity of phasic contraction in the ABRM of Mrtilus edulis.

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Change of External Reproductive Parameters according to Sexual Maturation of the Striped Bitterling, Acheilognathus yamatsutae(Teleostei: Acheilognathinae) (줄납자루, Acheilognathus yamatsutae의 성 성숙에 따른 외부생식형질의 변화)

  • Jin, Young Guk;Kim, Chi Hong;Lee, Chul Woo;Kim, Jae Won;Lee, Jung Sick
    • Korean Journal of Ichthyology
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    • v.20 no.1
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    • pp.13-20
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    • 2008
  • Internal and external reproductive parameters of the striped bitterling, Acheilognathus yamatsutae were compared. Internal reproductive parameters was used gonadosomatic index (GSI), hepatosomatic index (HSI) and gonadal development stage. External reproductive parameters was used ovipositor in female and pearl organ, dorsal and caudal fin nuptial color in male. The GSI and HSI of the female and male showed highest in February. Gonadal development was observed mainly ripe and spawning/spent stage in February to May. The ovipositor length index (OLI) of the female showed highest in March to April. Pearl organ number and expression rate of the male showed highest in February to May. Dorsal fin nuptial color expression rate and index (NCI-DF) showed highest in February to May. Caudal fin nuptial color expression rate and index(NCI-CF) showed highest in February to April. The study suggested same change of internal and external reproductive parameters in female and male.

Seepage Characteristics of Embedded Rock Layer Under the Earth Fill (성토제 하부에 매설된 사석층의 침투특성)

  • Lee Haeng-Woo;Chang Pyoung-Wuck
    • Journal of the Korean Geotechnical Society
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    • v.21 no.8
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    • pp.63-72
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    • 2005
  • Rocks are dumped to soft marine ground in order to improve trafficability and construction conditions in the tideland reclamation construction sites. Though this rock layer under earth fill has caused in a serious seepage problems after construction, seepage behaviors of this embankment structure is not correctly investigated. Water flow through rock layers is, in general, known as Non-Darcy's flow. However, the embedded rock layer under earth fill is not known whether its flow is governed by Darcy's or Non-Darcy's law. Therefore, a numerical analysis, laboratory model test and filed investigations were performed for analyzing the those seepage characteristics in this research. Results show that there is significance of $95\%$ of confidence between observed heads and seepage rates, and the calculated ones by SAMTLE which is developed under the assumption that the water flows through the two-layer system obey the Darcy's flow. And after operating the hydraulic gradient(i) of $0.10\~0.55$ upon laboratory model, these seepage characteristics of the embedded rock layer show that Reynolds Numbers are less than 10 and the relationship between these velocities of rock layer(v) and hydraulic gradients(i) is linearly proportional with more than 0.79 of the coefficient of correlation $(R^2)$. And the Reynolds Number of the velocity calculated by the relation of v=ki in the embedded rock layer of OO sea dike is $1\~6$. It shows also laminar flow. Based on these results, it is concluded that the seepage characteristics of embedded rock layer under earth fill can be laminar and Darcy's flow.

Studies on the Korean Deep Sea Fishing Industry Administration -Analysis of present situation and prospects- (한국의 해외 원양어업 경영에 관한 연구 - 현황 분석과 전망 중심으로-)

  • 김우성
    • The Journal of Fisheries Business Administration
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    • v.1 no.2
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    • pp.1-22
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    • 1970
  • Our fisheries protucts industry has developed rapidly during past 10 years ; production was about twice: fihing fleets increased twice in number and 3 times in tonnage ; export was 5 times. Govermment is trying to develop deep sea fisheries in order to surmount the depression of coast fisheries. At present more than 270 deep sea fishing boats are working with superior skill to other country at the South Pacific, the Atlantic, the Indian, and the North Pacific Ocean. Our deep sea fisheries is consisted of the tuna long line and the trawler. The tuna long line of them has 230 crafts in 1969 ana the deep sea trawler has 40 crafts, too. Comparing to 1962, the number of the deep sea fishing boats has been increased highly to 54 times, 7.71 times average per year increasing rate. The rate of the tuna long line to the trawler at the end of 1969 shows 85:15, More than half of them are 100~200 (equation omitted), if we classify them according to (equation omitted) or boat craft. 70% of them has less than 5 years ships age. The Korean Marine Industry Development Corporation has more than 1/3 fishing fleets, with 91 crafts, if We consider it according to corporation. Considering it according to the financial resources, dependence upon foreign loan is as high as 88%. Catches was 74, 450 M/T ($24, 663, 000)at the end of November in 1969 and it was increased to 113.5 times in catch amount and 118 times in value, comparing to those of 1962. Considering it according to the ocean, the order is arranged to the Pacific, the Atlantic, and the Indian Ocean. The average production amount of each craft is 250~400 S/T a year. The result of export took up 1/3 of total fisheries product export with $ 22, 398, 000 at the end of november in 1969. Employee cost of fishing coast is 8% higher than other fishing. The profit is highest in our fisheries. Most of the products except the trawler fishing are sofa at the fishing grounds to the processing company, and they lose much money. They buy most of bait from Japan, giving $8~10 for 1C/S(10kg). Fish price is $390~520 according to the kind of fish for S/T at the fishing grounds, and the rapid frozen fishes brought to Japan are about $ 800 for S/T. There is much difference. in price. Problems 1. Want of self capital. 2. To get the refrigeration boats enough. 3. International Fishing Regulation. 4. To get high price and to secure consuming grounds. 5. To get home-production of bait. 6. To exploit new fishing grounds. 7. To get larger boats. 8. To get mariner enough. When the problems mentioned above are solved, the Deep Sea Fisheries of oun courtry will be developed more largely.

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Characteristics of Korean Inshore Fishing Boats (한국(韓國) 연근해어선(沿近海漁船)의 특성(特性))

  • Keuck-Chun,Kim
    • Bulletin of the Society of Naval Architects of Korea
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    • v.8 no.1
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    • pp.67-94
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    • 1971
  • Korean fisheries industry holds 57,255 boats amounting to 269,079 tons in gross as of the end of 1967. The boats of the size under 30G.T., about sixty-two per cent of the total in tonnage, are utilized in coastal and their contribution to the total production of Korean fisheries industry is estimated at over seventy-five percent. Hull forms and construction method of them are partly in transition stage to the western tradition from the oriental tradition, which employs the chine-type straight-framed section, and tick and wide wooden planking fastened on naturally bent timber frames. And only about a half of them are mechanized. About fifty-seven per cent, 7,525 boats amounting to 91,751 gross tons, of the coastal and inshore fishing boats are those of the size ranging 5G.T. to 30G.T., which are engaged mostly in draft-and gill-netting, angling, longlining, anchovy seining, squid fishing and set-netting. The important fishing boats forming main structure of the inshore fishing fleets can be classified as 5G.T.-class multi-purpose boats, 10G.T.-class angling/longlining boats and 20G.T.-class drift-/gill-netters mostly utilized in the east-sea, 15G.T.-class anchovy seiner in the south-sea-sea, 20G.T.- and 28G.T.-class stow-netters in the west sea. Each of the three sea regions, east, south, and west, of Korean water has distinctly different characters from one another in topography, geology and sea aspects, and consequently in marine resources, fishing grounds and fishing tactics desired. Hence, the finishing boats in each sea region have also their own characters in hull form, structural features, deck design and equipments. In this report, analyzing the characteristics of the existing inshore fishing boats ranging 5G.T. to 30G.T. in size from view points of naval architecture and engineering, the author made an integrated investigation of their characteristics, covering size and principal dimensions, hull form, deck design, structural features and mechanization, in close connection with the natural circumstances of Korean water and local techno-socio-economic problems, and, finally, made some suggestions for the rational improvement or modernization of the inshore fishing boats. Further details of the characteristics of Korean inshore finishing boats are referred to the drawings of them compiled by the author and given in the reference[23] listed at the end of this report.

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Application of SP Survey and Numerical Modeling to the Leakage Problem of Irrigation facilities (수리시설물 누수탐지에 대한 자연전위법 적용 및 수치 해석)

  • Song Sung-Ho;Kwon Byung-Doo;Yang Jun-Mo;Chung Seung-Hwan
    • Geophysics and Geophysical Exploration
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    • v.5 no.4
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    • pp.257-261
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    • 2002
  • We have carried out integrated research including field survey and numerical modeling to appraise the applicability of SP method to the leakage problems of irrigation facilities. The leakage pattern of the dike studied here can be classified into the three categories: leakage through the abutment, leakage by piping through dike, and leakage due to the composite effects of landslide and distortion of the dike structure. for the numerical modeling to interpret quantitatively SP survey results acquired at dike, we have modified the computer code proposed by Sill (1983) to apply to the leakage problems. The numerical studies match the characteristic patterns of SP anomalies according to the leakage types and appear to be very useful to interpret the leakage zone and path. The SP monitoring results were also well coincided with tidal variations observed at every embankment so we found the SP method is quite effective not only to detect the leakage zone but also to determine the leakage trend. The numerical modeling results also reproduced the SP anomalies due to seawater leakage in the embankment.

The Comparison of Extraction Process for Enhancement of Immunomodulating Activities of Ulva pertusa kjellman (구멍갈파래의 면역활성 증진을 위한 추출방법 비교)

  • Han, Jae-Gun;Ha, Ji-Hye;Choi, Yeong-Beom;Go, Jeong-Lim;Kang, Do-Hyung;Lee, Hyeon-Yong
    • Korean Journal of Food Science and Technology
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    • v.41 no.4
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    • pp.380-385
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    • 2009
  • The purpose of this study was to investigate the immunomodulatory effect of Ulva pertusa kjellman extract after undergoing a low temperature and high-pressure extraction process. First, the extracts obtained under the extraction conditions of 150 MPa and $80^{\circ}C$showed a relatively high antioxidant activity, with 90% super oxide radical activity compared to the extracts from conventional extraction process with water at $100^{\circ}C$. This extract also improved the growth of both human immune B and T cells up to $14.5{\times}10^4$ cells/mL and $14.2{\times}10^4$ cells/mL compared to $9.1{\times}10^4$ cells/mL in adding the extracts from conventional processes. It was found that the extracts obtained at 100 MPa and $60^{\circ}C$ showed better activities in NK cell growth and NO production from macrophage as $11.8{\times}10^2$ cells/mL and 30.0 ${\mu}M$. Overall, the extracts from high pressure and low temperature extraction process had relatively higher immune activation activity, possibly because the low temperature and high pressure extraction process may have higher yields of active compounds and have less damage to useful ingredients from relatively weak marine natural resources, such as Ulva pertusa kjellmann than that from the conventional extraction system.

Preliminary Study on the Paleoenvironment Reconstruction using a Bivalve from Sangmori Shell Mound (상모리패총에서 산출되는 이매패류 패각을 이용한 고환경 복원 예비연구)

  • Kim, Jeong-Suk;Woo, Kyung-Sik;Hong, Wan;Kang, Chang-Hwa
    • The Korean Journal of Quaternary Research
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    • v.24 no.2
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    • pp.35-48
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    • 2010
  • The purpose of this study is to investigate paleoenvironmental conditions of the shallow sea around Jeju Island during the Late Holocene using geochemical contents of the bivalve (Glycymeris albolineata) collected from the Sangmori Shell Mound. The bivalve shell used shows the archaeological age of 2,300 yr BP. Stable carbon and oxygen isotope compositions show that growth rates decreased with aging. Coeval trends of both isotope compositions can be observed: heavier values during winters and lighter values summers except for their young and old growth stages. The seasonality of bivalve shell appear to reflect seasonal variations of paleotemperature as well as paleosalinity. Especially China Coastal Water with low salinity was transported into the southern Jeju Strait from Changjiang River during summer periods. Heavier carbon isotope values during winter indicate higher productivity, and this is supported by high density of phytoplanktons and higher chlorophyll contents during winter time. For accurate interpretation, monitoring of present-day conditions of shallow marine water as well as additional geochemical analysis of the same Recent bivalve may be necessary.

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Isolation and Degradation Activity of a TBTCl (Tributyltin Chloride) Resistant Bacteriain Gwangyang Bay (광양만에서 TBTCl (Tributyltin Chloride) 내성세균의 분리 및 분해활성)

  • Jeong, Seong-Yun;Son, Hong-Joo;Jeoung, Nam-Ho
    • Korean Journal of Environmental Agriculture
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    • v.30 no.4
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    • pp.424-431
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    • 2011
  • BACKGROUND: Tributyltin chloride is among the most toxic compounds known for aquatic ecosystems. Microorganisms are responsible for removal of TBTCl. Nevertheless, only a limited number of marine bacteria were investigated for biodegradation of TBTCl in Korea. METHODS AND RESULTS: The number of TBTCl resistant bacteria ranged from $2.5{\times}10^3$ to $3.8{\times}10^3$ cfu/mL in the seawater, and ranged from $3.2{\times}10^5$ to $9.1{\times}10^5$ cfu/g in the surface sediment, respectively. The morphological, physiological, and biochemical characteristics of TBTCl resistant bacteria were investigated by API 20NE and other tests. The most abundant species of TBTCl resistant bacteria were Vibrio spp. (19.2%), Bacillus spp. (16.2%), Aeromonas spp. (15.2%), and Pseudomonas spp. (13.1%), etc. Eleven TBTCl resistant isolates also had a resistance to heavy metals (Cd, Cu, Hg, and Zn). Among them, isolate T7 showing the strong TBTCl-resistance was selected. This isolate was identified as the genus Pantoea by 16S rRNA gene sequencing and designated as Pantoea sp. T7. In addition, this bacterium was cultivated up to the growth of 50.7% after 60 hrs at TBTCl concentration of $500{\mu}M$. TBTCl-degrading activity of Pantoea sp. T7 was measured by GC-FPD analysis. As a result of biological TBTCl-degradation at TBTCl concentration of $100{\mu}M$, TBTCl-removal efficiency of Pantoeasp. T7 was 62.7% after 40 hrs. CONCLUSION(S): These results suggest that Pantoea sp. T7 is potentially useful for the bioremediation of TBT contamination.

One-dimensional XRD-Pattern Calculations of Clay Minerals: I. Dioctahedral and Trioctahedral Fe-rich Smectites (粘土鑛物의 一次元的 X-線 回折圖形의 計算: I. Dioctahedral 과 Trioctahedral Fe-rich 스멕타이트)

  • Ahn, Jung-Ho
    • Journal of the Mineralogical Society of Korea
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    • v.4 no.1
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    • pp.11-21
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    • 1991
  • XRD patterns of biedellite-nontronite and saponite-iron saponite series were investigated using one-dimensional pattern simulation method. Ethylene-glycolate smectites show stronger 002 and 003 reflections than hydrated specimens do. The intensities of the 002 and 003 reflections change systematically as a function of Fe enrichment in both types of smectites. The intensity ratio of 002/003 increases with increasing. Fe in both dioctahedral to the higher scattering factor of Fe than those of Al and Mg, and the scattering power of various smectites can be compared quantitatively by calculating the scattering factors of octahedral cations. Interlayer cations cause less effect on XRD profile than octahedral cations as Fe do. Although 001 reflections provide informations about the overall scattering power of the octahedral sheet, some ferrous dioctahedtal smectite cannot be distinguished unambiguously from trioctahedral smectites on the basis of XRD profile. Simulation showed that heterogeneous smectites exhibit 001 intensity distribution that is almost identical to that of homogeneous smectites having the average composition of heterogeneous ones. The broadening of 001 reflections may not be useful in evaluating the degree of heterogeneity of smectite unless other factors affecting the broadening are well known.

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