• Fisheries and Aquatic Sciences
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• v.25 no.5
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• pp.264-275
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• 2022

A non-destructive environmental DNA protocol for the genetic analysis of sea cucumber (Apostichopus japonicus) resources DNA was established. Among the several commercial DNA extraction kits, the DNeasy^® Plant Mini Kit was selected as the best choice to obtain the high-quality genomic DNAs from the mucous sea cucumber. As the temperature and incubation time increased, the amount of extracted environmental DNA was also large, but it was judged that the increased amount did not affect as much as 2-3 times. Therefore, these conditions were not considered to be the main factors to consider in actual environmental DNA extraction. However, the amount of seawater relative to the size of the sample was judged as a major consideration, and a sufficient amount of environmental DNA for analysis was secured when stored within 1 min while stirring the volume of seawater corresponding to the total sea cucumber weight (g). In securing the environmental DNA of sea cucumbers, the mortality rate of sea cucumbers in all experiments was 0, and it was judged that the effects of sea cucumbers were not significant through this treatment. Through the results of this study, sea cucumber DNA research, which has been conducted in a destructive method, can be conducted non-destructively through environmental DNA analysis. Through this study, we have secured a standard protocol that can successfully extract the sea cucumber DNA through environmental DNA. It is not only excellent in terms of time and cost of traditional DNA analysis method currently used, but it is completely non-destructive in the ecosystem of the survey area. It is believed that the system can be transformed in a way that does not affect it. However, it is thought that various standard protocols should be established considering the characteristics of each type.

• Korean Journal of Ichthyology
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• v.32 no.2
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• pp.97-102
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• 2020

The feeding habits of larval Ammodytes japonicus were examined. A total of 208 individuals were collected by RN80 Net from January to February, 2019 in the coastal waters of Gujora and Mangchi, Geoje, Korea. Range of notochord length of larval A. japonicus was 1.46~8.00 mm and fed mainly on copepods that constituted 99.9% in %IRI. The results of analysis in ontogenetic changes, divided with 3 classes (≤2 mm, 3~4 mm, ≥5 mm, Notochord length), was all classes mainly fed copepods. An opposite relationship was found between the calanoida and cyclopoida. When percentage of calanoida of copepods increased, cyclopoida of copepods decreased.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.56 no.2
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• pp.94-104
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• 2020

An ecological study for species composition and abundance of the fish assemblages in the coastal waters of Gujora and Mangchi of Geoje, Korea was conducted using monthly larval collection by an RN80 net from June, 2018 to May, 2019. A total of the 5 Orders, 15 Families, 21 species were collected. The dominant species were Ammodytes japonicus and Engraulis japonicas. Gobiidae was the most dominant species in June, Spratelloides gracilis in July, Chelidonichthys sp. in August, Gobiidae in September, Repomucenus beniteguri in October, Sebastiscus marmoratus in November, Sebastiscus marmoratus and Hexagrammos otakii in December of 2018, Pleuronectes yokohamae in January, Ammodytes japonicus in Febraury, Engraulis japonicus in March, Sebastes sp. and Gobiidae in April and Engraulis japonicus in May of 2019. The highest number of species and individuals were found in July and the smallest in August. Thus, the diversity index was the highest in July and the lowest in August. As a result of the similarity by month was divided into Group I in January and February and Group II in April, June and September. The most dominant species in the Group I were the Ammodytes japonicus and Sebastes inermis, and the most dominant species in the Group II was Gobiidae.

• Proceedings of the IEEK Conference
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• 2009.05a
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• pp.316-319
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• 2009

Currently for exploring marine resources, After confirming the location of the resources by controlling search equipment and sensor module in research vessel, the method which collects sample of searched resources and analyzes the quality and a contents of the resources in research vessel is been applying. This search method is structure which analyzes an ingredient in ship and decides the quality of the resources. And real-time processing for an effective resources search is been demanding. Therefore in this paper, we have developed a resources identification algorithm that can display the type feature of resources by real-time 3D Graphic.

• Ocean Science Journal
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• v.43 no.2
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• pp.101-106
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• 2008

A single specimen of Albula leptocephalus (55.7 mm SL) was collected from the southern coastal waters of Korea using an aquatic lamp. It is characterized by having a ribbon-like body with a small head and a well-forked caudal fin. Although the general appearance was similar to the leptocephalus of A. vulpes including myomere counts and fin ray counts, the melanophore deposition was different from that of A. vulpes. This leptocephalus specimen was confirmed with A. forsteri using the cytochrome b mtDNA (Cytb) analysis. The genetic distance of Cytb between the present leptocephalus and A. forsteri is 0.006-0.038, which falls into the cutoff point separating Albula species into eight deep lineages including the four valid species. Its genetic characteristic have more similarities to those of Fiji than those of Hawaii and the Northern territory of Australia.

• Proceedings of the Korean Quaternary Association Conference
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• 2004.06a
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• pp.20-23
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• 2004

• 한국지구물리탐사학회:학술대회논문집
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• 2009.10a
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• pp.128-128
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• 2009

• Proceedings of the Korean Quaternary Association Conference
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• 2004.06a
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• pp.8-10
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• 2004

• 한국지구물리탐사학회:학술대회논문집
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• 2004.06a
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• pp.170-175
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• 2004

• The Korean Journal of Petroleum Geology
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• v.2 no.2 s.3
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• pp.101-104
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• 1994