• ALGAE
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• 제37권3호
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• pp.239-247
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• 2022

Enzyme-assisted hydrolysis is frequently used as a cost-effective and efficient method to obtain functional ingredients from bioresources. This study involved the enzyme-assisted hydrolyzation and purification of fucoidan from Ecklonia maxima stipe and the investigation of its anti-inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Fucoidans of Viscozyme-assisted hydrolysate from E. maxima (EMSFs) harvested in Jeju, Korea. Structural and chemical characterizations were performed using fourier transform infrared spectroscopy, scanning electron microscope, and monosaccharide analysis. Among fucoidans, EMSF6 was rich in fucose and sulfate and had a similar structural character to commercial fucoidan. EMSF6 showed a strong inhibitory effect on nitric oxide generation in LPS-induced RAW 264.7 cells and significantly decreased the production of LPS-induced pro-inflammatory cytokines, including interleukin-6, interleukin-1β, and tumor necrosis factor α. The anti-inflammatory potential of EMSF6 was mediated through the downregulation of inducible nitric oxide synthase and cyclooxygenase-2 expression. Thus, fucoidans from E. maxima stipe are promising candidates for functional food products.

• 한국수산과학회지
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• 제39권2호
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• pp.85-93
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• 2006

This study was conducted to evaluate the optimal processing conditions of smoke-dried powdered oysters and to determine their shelf-life during storage for development of a natural oyster flavoring substance. The optimal conditions for processing of smoke-dried oyster powder with freshy oyster were as follows. Raw shelled oysters were rinsed with 3% saline solution, drained, boiled for 10 minutes at $98^{\circ}C$, and then smoked for 1 hour at $50^{\circ}C$, followed by drying for 4 hours at $80^{\circ}C$ Smoke-dried oyster powder with oyster scraps were prepared as flavoring material. The smoked oyster scraps were submerged in oyster sauce far 10 minutes at room temperature and then dried with hot air for 5 hours at $50^{\circ}C$. The smoke-dried oysters and smoke-dried oyster scraps were then pulverized to 50 mesh and packed in tea bags or vacuum-packed in laminated plastic film bags (PE/PVDC/CPP, $12{\mu}m/15{\mu}m/50{\mu}m$). Compared to non smoke-dried powdered oysters, the smoking and dipping in oyster sauce enhanced the flavor and prevented lipid oxidation of the smoke-dried powdered oyster product. Shelf-life tests indicated that the vacuum-packaging method preserved the quality of smoke-dried powdered oysters stored for 150 days at room temperature.

• 한국융합학회논문지
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• 제13권1호
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• pp.189-199
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• 2022

현대의 일러스트레이션 디자인은 독창성을 필요로 하는데, 해양생물의 형태와 색채는 일러스트레이션 창작에 풍부한 소재를 제공한다. 일러스트레이션은 디자인적 창조 가치가 매우 높다. 본 연구는 해양생물을 거울삼아 색채학적 지식과 시각적 형태설계를 통해 이를 밝히고, 일러스트레이션 디자인과 해양생물을 주요 연구대상으로 하였다. 본 연구목적은 ① 해양생물의 전형적 특징을 색과 형태 중심으로 정리, ② 이를 해양생물을 대표하는 그래픽 기호로 도출하여, ③ 일러스트레이션으로 접목할 수 있음을 해양생물 일러스트레이션 제작으로 사례를 제시하였다. 연구를 통해서 결론을 도출하고, 해양생물과 육지생물을 구분하는 전형적인 특징 중 하나로 곡선적인 특징을 부각시켰다. 열대어 색채 현상에 대한 분류 연구와 비교분석을 통해 도출한 열대어류의 색채 특성을 바탕으로 해수 열대어의 색채를 추출 및 가공하여 일러스트레이션 디자인에 활용할 수 있다.

• Fisheries and Aquatic Sciences
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• 제17권3호
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• pp.275-290
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• 2014

The marine ecosystem represents a vast and dynamic array of bio-resources attributed with its huge diversity and considered as potential untapped reservoirs for the development of functional foods for future health markets. Basically, marine microorganisms, sponges, algae, invertebrates such as crustaceans and mollusks along with marine fish species can be considered as marine bio-resources, which can be utilized to obtain different health benefits for humans, directly or after processing. Most of the bio-molecular components, such as lipids and proteins from these marine bio-resources, which can be extracted in large scale using the modern and advanced biotechnological approaches, are suitable drug candidates for the pharmaceutical industry as well as functional food ingredients for the food industry. Moreover, the furtherance of high throughput molecular biological techniques has already been incorporated with identification, mining and extraction of molecular components from marine bio-resources. In this review, potential marine bio-resources with respect to their extractable bio-molecules were described in details, while explaining the present and prospective methods of identification and extraction, which are integrated with advanced techniques in modern biotechnology. In addition, this provides an overview of future trends in marine biotechnology.

• 한국해양바이오학회지
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• 제7권1호
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• pp.28-34
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• 2015

We examined cell regeneration efficiency of brown marine algae living in Jeju coast for search of a novel therapeutic device with cutaneous wound healing materials. The five algae were collected and compared with epidermal growth factor (EGF) as a positive control in the assays of cell proliferation and cell migration of NIH3T3 fibroblast cells. Among the 80% methanol extracts of these brown algae, the two algal extracts from Ishige foliacea and Colpomenia bullosa showed the proliferative effects of the cells similar to the effect of EGF. Besides it was found that Colpomenia bullosa extract significantly enhanced cell migration of NIH3T3 cell. In the study, therefore, we confirmed that the Colpomenia bullosa extract improved proliferation of NIH3T3 cell and a potential candidate for cultaneous wound healing.

• 한국어병학회지
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• 제28권2호
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• pp.99-107
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• 2015

Despite its economic importance as an aquaculture species, the molecular and genetic information regarding physiologically important elements in rock bream (Oplegnathus fasciatus) is not completely understood. Rab proteins play a vital role in cellular mechanisms and immunity as one of the key regulators of membrane trafficking. In this investigation, a Rab gene, named as RbRab-5C-like, was identified from Oplegnathus fasciatus. RbRab-5C-like protein exhibited high homology with Rab proteins of other species and possessed signature characteristics of Rab proteins with four conserved cysteine residues. Phylogenetic analysis showed that RbRab-5C-like clustered with other fish counterparts. The RbRab-5C-like genomic sequence possesses six exons and five introns. Transcriptional analysis revealed that RbRab-5C-like was ubiquitously expressed in all examined tissues with the highest expression occurring in the liver. While the structural and homologic characteristics of RbRab-5C-like suggest a strong conservation of this element in different species, its mRNA distribution implies a wide range of biological significance in rock bream.

• Natural Product Sciences
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• 제13권2호
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• pp.132-134
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• 2007

An analog of antibacterial sulfated alkene against Bacillus subtilis was isolated from a species of tunicate (Mideoduck), Styela clava, cultured at Jindong Bay, Korea. The structure was determined as 4,8-dimethyl-3-nonenyl sulfate by MS and spectral analysis such as UV IR and NMR.

• 한국어류학회지
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• 제34권4호
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• pp.253-261
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• 2022

부유사가 어류에 미치는 생리학적 특성을 알아보기 위해 대조구(0 mg/L), 100 mg/L, 250 mg/L, 500 mg/L, 1,000 mg/L인 농도 구간에 넙치와 조피볼락을 노출시킨 후 AST/GOT, ALT/GPT, ALP와 cortisol을 분석하였다. 분석 결과 부유사 농도 구간에서 AST/GOT, AL T/GPT, ALP와 cortisol 값이 대조구에서 보다 높게 나타났다. 부유사에 노출된 넙치에는 S. parauberis, 조피볼락에는 V. harveyi를 인위감염하였을 때 생존율은 부유사 농도 구간에서보다 대조구에서 더 높게 나타났다. 이러한 결과는 이전에 연구되었던 부유사가 어류에 악영향을 미친다는 결과와 연관되어지며, 어류의 건강도에 부유사가 영향을 주는 것으로 시사된다.

• 한국식품위생안전성학회지
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• 제36권6호
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• pp.455-473
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• 2021

이 총설에서는 해양 연체동물 내 독소 분석을 하는 연구자들의 이해를 돕기 위하여, 국내에서 생산되거나 유통되는 수산물 중 해양 생물독소에 오염될 수 있는 이매패류와 복족류 종들의 공식 명칭과 생태학적 특성을 소개하였다. 또한 대표적인 생물군의 해부도를 통한 각 기관들의 위치와 공식적인 명칭을 알리고자 하였다. 향후 해양생물독소 분석 관련 식품공전, 학술논문 및 보고서에 실험생물의 정확한 종명과 분석 기관의 명칭에 대한 통일 및 규격화가 필요하다.

• ALGAE
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• 제34권1호
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• pp.45-56
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• 2019

Ecklonia cava (EC) has been widely utilized as an ingredient in commercial products such as functional foods and cosmeceuticals. Recently it has been found that Sargassum horneri (SH) has been invading on Jeju Island coast area by its huge blooming. Moreover, both seaweeds are considering as important ingredients in traditional medicine specifically in East-Asian countries (China, Japan, and Korea). In the present study, we attempted to compare anti-inflammatory properties of 70% ethanolic extracts of EC (ECE), SH (SHE), and their different combinations on lipopolysaccharide (LPS)-activated RAW 264.7 cells. Results indicated that 8 : 2 combinations of ECE : SHE significantly inhibited LPS-activated inflammatory responses (cytokines, protein, and gene expression) in RAW 264.7 macrophage cells compared to the respective extracts and other combinations. The synergistic effect of ECE and SHE was found to be prominent than the effects of ECE or SHE alone. These observations provide useful information for the industrial formulation of functional materials (functional foods and cosmeceuticals) using these two particular seaweeds in Jeju Island of South Korea.