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http://dx.doi.org/10.7847/jfp.2015.28.2.099

Genomic identification and spatial expression analysis of Rab-5C-like gene identified from rock bream(Oplegnathus fasciatus)  

Mothishri, M.S. (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University)
Umasuthan, Navaneethaiyer (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University)
Thulasitha, William Shanthakumar (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University)
Whang, Ilson (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University)
Lee, Jehee (Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University)
Publication Information
Journal of fish pathology / v.28, no.2, 2015 , pp. 99-107 More about this Journal
Abstract
Despite its economic importance as an aquaculture species, the molecular and genetic information regarding physiologically important elements in rock bream (Oplegnathus fasciatus) is not completely understood. Rab proteins play a vital role in cellular mechanisms and immunity as one of the key regulators of membrane trafficking. In this investigation, a Rab gene, named as RbRab-5C-like, was identified from Oplegnathus fasciatus. RbRab-5C-like protein exhibited high homology with Rab proteins of other species and possessed signature characteristics of Rab proteins with four conserved cysteine residues. Phylogenetic analysis showed that RbRab-5C-like clustered with other fish counterparts. The RbRab-5C-like genomic sequence possesses six exons and five introns. Transcriptional analysis revealed that RbRab-5C-like was ubiquitously expressed in all examined tissues with the highest expression occurring in the liver. While the structural and homologic characteristics of RbRab-5C-like suggest a strong conservation of this element in different species, its mRNA distribution implies a wide range of biological significance in rock bream.
Keywords
Rab-5C-like; Membrane trafficking; Phylogenetic analysis; Transcriptional analysis;
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