• 한국수산과학회지
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• 제50권2호
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• pp.175-182
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• 2017

We examined the effects of environmental factors on zoospore release and germling growth of the green tide alga Cladophora albida under various conditions of temperature${\times}$irradiance (zoospore release), temperature${\times}$irradiance${\times}$ nutrient (germling growth), and a single factor test of salinity. Zoospore release was maximized at $30^{\circ}C$ and $100{\mu}mol\;photons\;m^{-2}s^{-1}$ in the temperature irradiance experiment and at 34 psu in the salinity experiment. Maximum germling growth was observed at $25^{\circ}C$ with $100{\mu}mol\;photons\;m^{-2}s^{-1}$ and PES (Provasoli's Enriched Seawater) in the temperature irradiance nutrient experiment, and at 34 psu in the salinity experiment. Germlings grew faster at higher irradiances for a given temperature level, and also grew faster as salinity increased over the range of 5-34 psu. Overall, optimal environmental conditions for zoospore release were $30^{\circ}C$, $100{\mu}mol\;photons\;m^{-2}s^{-1}$ and 34 psu. Maximal germling growth occurred at $25^{\circ}C$, $100{\mu}mol\;photons\;m^{-2}s^{-1}$, PES, and 34 psu. C. albida blooms are most likely to occur under these optimal environmental conditions, as plentiful zoospore release and rapid germling growth lead to population growth.

• ALGAE
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• 제21권3호
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• pp.317-321
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• 2006

The effects pH and salinity on the brown alga Sargassum fulvellum were investigated in the context of the removal of two major associated animals, Caprella scaura and Gammaropsis utinomi. Optimum quantum yield (Fv/Fm) of S. fulvellum was also examined in the same experimental conditions as an index of stress. Experiments on pH and salinity tolerances of the two animal species indicated that mortality was more than 80% at extremes of pH (2, 3, 4, 10, 11, 12) and salinity (0, 3.5, 7, 10, 44 psu) after a 5 min treatment. Lethal time (LT50) from pH 2 to pH 4 was less than 90 sec in C. scaura, and less than 70 sec in G. utinomi. From 0 to 10 psu, LT50 was less than 20 sec in C. scaura and less than 60 sec in G. utinomi. The quantum yield of S. fulvellum was not significantly different from controls within the pH range 4~10, and within the salinity range of 7-40 psu. When exposed to pH and salinity conditions outside these ranges, the effect of these factors on the removal of two animal species was higher, but quantum yield was highly reduced. These results indicate that the optimal conditions for removing the animal species without affecting optimum quantum yield were pH 4-10, and salinities 7-10 psu and 44 psu.

• 한국수산과학회지
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• 제32권1호
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• pp.117-120
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• 1999

해산 녹조류 참흩파래, Monostroma nitidum의 엽체를 효소처리하여 다량의 원형질체를 분리하였다. 최적 효소액의 조합은 $4\%$ R-10+$3\%$ Macerozyme R-10+$3\%$ Abalone acetone power로서 생체조직 300mg 당 $4.41\times10^6$개의 원형질체가 분리되었다. 원형질체의 수율은 효소처리 270분에 최대였다. 분리 직후의 원형질체는 구형으로 직경 $13\~33\mu$m의 크기였다. 분리된 원형질체는 0.4M mannitol을 함유한 f/2배지에서 배양한 후 매주 mannitol이 함유되지 않은 f/2 배지로 절반씩 교환함으로서 분화율을 높일 수 있었다. f/2배지를 사용한 적정 배양조건에서 원형질체는 배양 3일 후 새로운 세포벽을 형성하였으며 10일 후 발아하기 시작하여 엽체로 발달하였다. 항생물질의 배지내 첨가는 배양체의 분화를 저해하였다.

• 한국미생물·생명공학회지
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• 제31권2호
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• pp.165-170
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• 2003

본 실험은 해수 미세 조류인 Chlorella capsuiata로부터 신경세포에 대한 활성을 증가시키는 기능성물질을 분리하여 해수자원의 생체 조절자원으로서의 가능성을 제시하고자 실시하였다. 선별된 해수 Chlorella를 이용하여 활성물질을 분리한 뒤, 그 물질의 신경활성을 탐색하였다. C. capsulata의 물 추출물로부터 분리된 분획물(CCE)의 분자량은 약 45KDa(data not shown)으로 기존에 연구된 60~100 KDa보다 더 낮은 범위에서 물질이 분리되었으며, 이는 현재 발표된 많은 연구결과에서 주장하는 물질들과는 다른 종류의 물질임을 제시하고 있어 이에 보다 심층적인 연구가 수행되어져야 한다고 생각된다. 실험 결과를 통해 볼 때 활성을나타낸 주된 물질은 C. capsulata의 수용성 성분으로 생각되어지며, 260 nm에서 최대 흡광도를 나타내는 물질로 C. capsulate에 존재하는 단백질이 열 변성에 의해 탄수화물과 결합한 glycoprotein의 형태로 존재하는 것으로 추측되지만 일부의 연구결과에서 280nm에 최대 흡광도를 보이는 활성물질이 glycoprotein이라고 주장하고 있어 이 분획물(CCE)에 대한 좀더 심도 깊은 연구가 수행되어져야 한다고 생각된다. 이는 최근에 발표된 Chiorella의 기능성과 관련한 논문들에서 언급한 내용들과 유사한 결과를 나타내지만 대부분이 담수조류에 대한 활성 탐색의 결과임을 감안한다면 본 실험을 통해 해수 미세조류로부터 분리된 물질의 새로운 활성물질로서의 가능성을 제시한 것이라 하겠다. 또한, 다른 유기용매를 통해 활성물질을 분리하는 것과 달리 순수한 물을 통해 Chlorella의 수용성 성분을 추출하는 것이 좀더 신경세포의 활성을 증가시킨다는 것을 확인하였다. 앞으로 이 수용성 물질에 대찬 면역활성과 in vivo 실험을 통해 좀더 깊은 연구가 수행되어지고, 나아가 대량배양기술, 분리정제 기술이 뒷받침된다면, 고비용의 동물세포를 이용한 신경활성물질을 대체할 새로운 신경 활성물질 개발은 물론 다방면에 걸친 생체 조절기능을 가진 기능성 소재로서 활용 범위가 점차 확되지 않을까 생각한다.

• 한국해양학회지
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• 제10권2호
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• pp.51-56
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• 1975

Continued to the previous papers, the present investigation is carried out to clarify cesium-137 activities and potassium contents among 54 samples of edible marine algae collected along the coast of Korea during September, 1973 and April, 1974. The potassium contents are variable, 1.5-29.6%, and 11.36% on an average, while most of the members contain them about 10-16% generally. Among the algal phyla, they are 13.49% in green, 10.82% in brown and 16.46% in red algae, respectively. Cesium-137 activities are variable, 0.19-8.13 pCi$\^$137/ Cs/g K, and 2.35 pCi$\^$137/Cs/g K on an average in the samples investigated. Among the algal phyla they are 4.35 in green, 2.17 in brown and 0.89 pCi $\^$137/Cs/g K in red algae, respectively. Green algae contain especially higher activities compared with the other two groups. The brown alga, Myelophycus caespitosus shows the highest activities, 8.13 pCi$\^$137/Cs/g K, among the members investigated. Considering the concentration factor, this plant would be an indicator plant of cesium-137 among the marine algae along the coast of Korea.

• 수산해양교육연구
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• 제29권2호
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• pp.552-559
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• 2017

To assess the effect of LED lights on marine microalgae growth in the laboatory, Tetraselmis suecica, Chaetoceros simplex and Isochrysis galbana were cultured under $20{\pm}1^{\circ}C$, Walne's medium and aeration using 3.6 L glass vessels. The LED light sources were Blue, Red, Blue+Red, CoolWhite and WarmWhite. The experiments were conducted three times. The density of microalgae was shown as the counted number of cells per day, and the specific growth rate was calculated by using the density. The statistical analysis was performed by analysis of variance using the SPSS 20.0 program. T. suecica culture was the highest density under the Blue LED light source, so the light source was the most effective for the growth of this alga. C. simplex and I. galbana culture had the highest density under the Blue+Red LED light source, therefore this light source was the most effective for the growth of these algae. The result of analysis of variance showed significant between groups.

• 한국해양바이오학회지
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• 제9권2호
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• pp.22-29
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• 2017

A unicellular green alga was axenically isolated from the Port of Jeongja, Ulsan, Korea. Morphological, molecular, and biochemical analyses revealed that the isolate belonged to Heterochlorella luteoviridis. This is the first report of this species in Korea. The microalgal strain was named as H. luteoviridis MM0014 and its growth, lipid composition, and biomass properties were investigated. The strain thrived over a wide range of temperatures ($5-30^{\circ}C$) and withstood up to 0.5 M NaCl. The results of gas chromatography/mass spectrometry analysis showed that the isolate was rich in nutritionally important polyunsaturated fatty acids. Its major fatty acids were linoleic acid (35.6%) and ${\alpha}$-linolenic acid (16.2%). Thus, this indigenous marine microalga is a potential alternative source of ${\omega}3$ and ${\omega}6$ polyunsaturated fatty acids, which are currently obtained from fish and plant oils. Ultimate analysis indicated that the gross calorific value was $19.7MJ\;kg^{-1}$. In addition, the biomass may serve as an excellent animal feed because of its high protein content (51.5%). Therefore, H. luteoviridis MM0014 shows promise for applications in the production of microalgae-based biochemicals and biomass feedstock.

• Fisheries and Aquatic Sciences
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• 제9권3호
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• pp.115-117
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• 2006

A micro algal growth-enhancing polysaccharide compound was isolated from the green alga Monostroma nitidum using water extraction, molecular fractionation, a DEAE-cellulose column, and fast protein liquid chromatography using a Superose-12 column. The yield of the compound from the seaweed powder was 8.3$\times$l0$^{-3}$%. At 2 mg/mL concentration, the polysaccharide enhanced Tetraselmis suecica cell growth in f/2 medium by approximately 160%.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권4호
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• pp.221-224
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• 2002

Biomass of non-viable and dried brown marine algae Ecklonia radiata Turn. was used to examine Its cadmium uptake capability. Twelve different pretreatments on the algal biomass were prepared. Among these pretreatments, the algal biomass, which treated with 0.1 M NaOH and kept in water. bath (100$\^{C}$, 18 h) followed by washing with distilled water and squeezing, showed the highest amount of cadmium uptake as 1634 $\pm$ 195 mg/g dry biomass at pH 4.0 and 50$\^{C}$. Adsorption temperatures and pH levels played some Important role In cadmium uptake. However, cadmium uptake decreased dramatically at a lower pH than 4.0. Freundlich adsorption isotherm showed potent cadmium uptake capacity of the non-viable biomass. Pretreatments on the non-viable algal biomass shown in this study nay enhance the Eadmium removal in the industrial wastewater.

• 수산해양교육연구
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• 제23권1호
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• pp.126-129
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• 2011

Vegetative morphological information of the red alga Neorhodomela larix (Turner) Masuda (Rhodomelaceae) is given. This species is characterized by thalli up to 15 cm high, brownish black in color, pericentral cells with transverse division, the first order subulate branches with determinate growth, numerous adventitious branches produced at axils and absence of vegetative trichoblasts. Particularly, N. larix is distinct from other Korean Neorhodomela species by the lack of vegetative trichoblasts, which seems to be caused by their delayed production until reproductive structures are formed. This is the first record of N. larix in Korea.