• International Journal of Industrial Entomology and Biomaterials
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• 제10권2호
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• pp.79-86
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• 2005

Molecular markers have increasingly been used in plant genetic analysis, due to their obvious advantages over conventional phenotypic markers, as they are highly polymorphic, more in number, stable across different developmental stages, neutral to selection and least influenced by environmental factors. Among the PCR based marker techniques, ISSR is one of the simplest and widely used techniques, which involves amplification of DNA segment present at an amplifiable distance in between two identical microsatellite repeat regions oriented in opposite direction. Though ISSR markers are dominant like RAPD, they are more stable and reproducible. Because of these properties ISSR markers have recently been found using extensively for finger printing, pohylogenetic analysis, population structure analysis, varietal/line identification, genetic mapping, marker-assisted selection, etc. In mulberry (Morus spp.), ISSR markers were used for analyzing phylogenetic relationship among cultivated varieties, between tropical and temperate mulberry, for solving the vexed problem of identifying taxonomic positions of genotypes, for identifying markers associated with leaf yield attributing characters. As ISSR markers are one of the cheapest and easiest marker systems with high efficiency in generating polymorphism among closely related varieties, they would play a major role in mulberry genome analysis in the future.

• International Journal of Advanced Culture Technology
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• 제4권2호
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• pp.1-6
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• 2016

Impervious surfaces are important indicators for urban development monitoring. Accurate mapping of urban impervious surfaces with observational satellites, such as VNREDSat-1, remains challenging due to the spectral diversity not captured by an individual PAN image. In this article, five multi-resolution image fusion techniques were compared for the task of classifting urban impervious surfaces. The result shows that for VNREDSat-1 dataset, UNB and Wavelet tranformation methods are the best techniques in reserving spatial and spectral information of original MS image, respectively. However, the UNB technique gives the best results when it comes to impervious surface classification, especially in the case of shadow areas included in non-impervious surface group.

• 생태와환경
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• 제43권4호
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• pp.477-491
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• 2010

Benthic macroinvertebrates were collected two times from 116 sites at the $1^{st}{\sim}4^{th}$ order streams in forest areas in Gyungsang province in late spring and late summer. The sample sites belonged to slightly and moderately polluted states. When communities were classified by the Self-Organizing Map (SOM), the gradient was observed according to degree of pollution. Within clusters of slightly polluted sites, however, seasonality was further observed. Scrapers, gatherer-collectors, and filterer-collectors were abundantly observed in late spring while shredders appeared more in late summer. The number of predator species increased in late summer. Behavior types were mostly clingers in two seasons. Community compositions at the moderately polluted sites were not much differentiated in different seasons. Gatherer-collectors and burrowers were dominantly collected in both seasons.

• 한국산림과학회지
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• 제95권5호
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• pp.580-584
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• 2006

This study was conducted to clarify the characteristics of breeding bird communities between deciduous and coniferous forests from April to June 2005 in Mt. Namsan, Seoul, Korea. Two 10ha areas were selected for territory mapping of breeding bird communities. Number of breeding bird species, pairs,density and bird species diversity index were higher in deciduous forest with increasing amount of foliage in the forest profile or as forest structures developed compared with coniferous forest. The number of in coniferous forest. The differences in habitat structure between both study areas are very likely to have influenced how breeding birds used the available habitat. Forest structure and its interactions with birds should be consideration in forest management for birds and their habitat.

• Asian-Australasian Journal of Animal Sciences
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• 제14권6호
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• pp.880-884
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• 2001

Rumen microbiology research has undergone several evolutionary steps: the isolation and nutritional characterization of readily cultivated microbes; followed by the cloning and sequence analysis of individual genes relevant to key digestive processes; through to the use of small subunit ribosomal RNA (SSU rRNA) sequences for a cultivation-independent examination of microbial diversity. Our knowledge of rumen microbiology has expanded as a result, but the translation of this information into productive alterations of ruminal function has been rather limited. For instance, the cloning and characterization of cellulase genes in Escherichia coli has yielded some valuable information about this complex enzyme system in ruminal bacteria. SSU rRNA analyses have also confirmed that a considerable amount of the microbial diversity in the rumen is not represented in existing culture collections. However, we still have little idea of whether the key, and potentially rate-limiting, gene products and (or) microbial interactions have been identified. Technologies allowing high throughput nucleotide and protein sequence analysis have led to the emergence of two new fields of investigation, genomics and proteomics. Both disciplines can be further subdivided into functional and comparative lines of investigation. The massive accumulation of microbial DNA and protein sequence data, including complete genome sequences, is revolutionizing the way we examine microbial physiology and diversity. We describe here some examples of our use of genomics- and proteomics-based methods, to analyze the cellulase system of Ruminococcus flavefaciens FD-1 and explore the genome of Ruminococcus albus 8. At Illinois, we are using bacterial artificial chromosome (BAC) vectors to create libraries containing large (>75 kbases), contiguous segments of DNA from R. flavefaciens FD-1. Considering that every bacterium is not a candidate for whole genome sequencing, BAC libraries offer an attractive, alternative method to perform physical and functional analyses of a bacterium's genome. Our first plan is to use these BAC clones to determine whether or not cellulases and accessory genes in R. flavefaciens exist in clusters of orthologous genes (COGs). Proteomics is also being used to complement the BAC library/DNA sequencing approach. Proteins differentially expressed in response to carbon source are being identified by 2-D SDS-PAGE, followed by in-gel-digests and peptide mass mapping by MALDI-TOF Mass Spectrometry, as well as peptide sequencing by Edman degradation. At Ohio State, we have used a combination of functional proteomics, mutational analysis and differential display RT-PCR to obtain evidence suggesting that in addition to a cellulosome-like mechanism, R. albus 8 possesses other mechanisms for adhesion to plant surfaces. Genome walking on either side of these differentially expressed transcripts has also resulted in two interesting observations: i) a relatively large number of genes with no matches in the current databases and; ii) the identification of genes with a high level of sequence identity to those identified, until now, in the archaebacteria. Genomics and proteomics will also accelerate our understanding of microbial interactions, and allow a greater degree of in situ analyses in the future. The challenge is to utilize genomics and proteomics to improve our fundamental understanding of microbial physiology, diversity and ecology, and overcome constraints to ruminal function.

• 정보관리연구
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• 제41권3호
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• pp.155-173
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• 2010

학문의 발전과 주제의 다양화로 인하여 각계의 연구자들은 자신에게 필요한 정보를 정확하게 찾을 필요성이 커지고 있다. 그리하여 본 논문에서는 효율적인 참고문헌 추출 방법으로 중복된 참고문헌을 비교 분석하여 자동으로 매핑해주는 시스템을 구축하고, 한의학 사전을 통한 한자의 오타를 교정할 수 있는 방법을 연구하였다. 이러한 방법을 적용함으로써 참고문헌의 중복입력과 한자오류를 개선할 수 있었다.

• 드라이브 ㆍ 컨트롤
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• 제17권3호
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• pp.33-46
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• 2020

We introduce a piece of special-purpose equipment for responding to disasters that has a dual-arm manipulator consisting of six-axis multi joints, and a master-slave operating system controlled by a wearable joystick for intuitive and convenient operation. However, due to the complexity and diversity of a disaster environment, training and suitable training means are needed to improve the interaction between the driver and equipment. Therefore, in this paper, a system that can improve the operator's immersion in the training simulation is proposes, this system is implemented in a virtual environment. The implemented system consists of a cabin installed with the master-slave operation system, a motion platform, visual and sound systems, as well as a real-time simulation device. This whole system was completed by applying various techniques such as a statistical mapping method, inverse kinematics, and a real-time physical model. Then, the implemented system was evaluated from a point of view of the appropriateness of the mapping method, inverse kinematics, the feasibility for real-time simulations of the physical environment through some task mode.

• 한국환경생태학회지
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• 제22권4호
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• pp.347-355
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• 2008

본 연구에서는 1990$\sim$2005년 사이에 일본전국의 109개의 주요하천유역에서 조사된 어류, 식물, 조류 데이터를 이용하여, 각 생물종의 종수와 기후 및 지형의 환경인자와의 관계를 분석하고 모델링을 수행하였다. 그 결과, 어류, 식물, 조류의 분포는 각각 연평균 기온, 유역면적, 연간 강수량에 의해서 가장 많은 부분이 설명되었다. 또한, 광범위 스케일에서의 환경인자데이터를 이용한 각 생물종에 대한 일반화가법모델을 이용하여 일본전국의 하천유역의 각 생물 종수의 값을 예측하고 그 값을 지도화하였다. 예측값을 지도화하는 것은 관리자가 생물종의 다양성을 확보하기 위해서 보호해야할 지역을 새롭게 설정하거나, 생물다양성 보호지역의 유효성을 평가하는데 활용될 수 있다.

• Mycobiology
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• 제45권2호
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• pp.105-109
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• 2017

Sixteen genomic DNA simple sequence repeat (SSR) markers of Lentinula edodes were developed from 205 SSR motifs present in 46.1-Mb long L. edodes genome sequences. The number of alleles ranged from 3-14 and the major allele frequency was distributed from 0.17-0.96. The values of observed and expected heterozygosity ranged from 0.00-0.76 and 0.07-0.90, respectively. The polymorphic information content value ranged from 0.07-0.89. A dendrogram, based on 16 SSR markers clustered by the paired hierarchical clustering' method, showed that 33 shiitake cultivars could be divided into three major groups and successfully identified. These SSR markers will contribute to the efficient breeding of this species by providing diversity in shiitake varieties. Furthermore, the genomic information covered by the markers can provide a valuable resource for genetic linkage map construction, molecular mapping, and marker-assisted selection in the shiitake mushroom.

• 제어로봇시스템학회논문지
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• 제15권6호
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• pp.619-625
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• 2009

FastSLAM which uses the Rao-Blackwellized particle filter is one of the famous solutions to SLAM (Simultaneous Localization and Mapping) problem that estimates concurrently a robot's pose and surrounding environment. However, the particle depletion problem arises from the loss of the particle diversity in the resampling process of FastSLAM. Then, the performance of FastSLAM degenerates over the time. In this work, DIR (Density Information-based Resampling) technique is proposed to solve the particle depletion problem. First, the cluster is constructed based on the density of each particle, and the density of each cluster is computed. After that, the number of particles to be reserved in each cluster is determined using a linear method based on the distance between the highest density cluster and each cluster. Finally, the resampling process is performed by rejecting the particles which are not selected to be reserved in each cluster. The performance of the DIR proposed to solve the particle depletion problem in FastSLAM was verified in computer simulations, which significantly reduced both the RMS position error and the feature error.