• Clinical and Experimental Reproductive Medicine
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• v.27 no.4
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• pp.345-351
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• 2000

Objective: Hatching of the blastocyst from the zona pellucida (ZP) is a key event in mammalian implantation. In vivo, two factors have been identified as possible mediators of hatching: lysis of the ZP by substances elaborated either from the embryo or female reproductive tract and pressure exerted on the zona by expansion of the blastocyst. Two methods of zona manipulation were already in use to enhance the ability of embryos to hatch: mechanical PZD and chemical ZD by acidic Tyrode's solution. But several controversies of each method have been reported. The purpose of this study was to investigate the effect of pronase for mouse embryo hatching. Methods: Mouse embryos were obtained following ovulation induction of $F_1$ animals. Fresh and cryo-thawed morula embryos were exposed to 0.5, 1.0, 2.0, 5.0 ${\mu}g/ml$ pronase in Ham's F10 for 72 hrs. Main outcome measures were the rates of partial hatching and completely hatched blastocysts, and cell number of it. Results: In fresh and cryo-thawed group, the rates of completely hatched blastocyst were significantly higher in 5 ${\mu}g/ml$ pronase treatment group than control group. There was no difference in completely hatched blastocyst total cell number between pronase treatment group and control group. This suggest that pronase treatment did not harmful in mouse embryo development. In pronase treatment group, zona pellucida were thinner than control group. Conclusion: The addition of pronase to culture media may accelerate the hatching of embryo. So, enzymatic treatment of the zona may provide a valuable and effective assisted hatching technique for human in-vitro fertilization-embryo transfer.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.1
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• pp.51-56
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• 2001

Changes of gill tissue and body composition of grey mullet (Mugil cephalus) and Nile tilapia (Oreochromis niloticus) by the manipulation of salinity were observed in a recirculating rearing system. Salinity was increased from $0\%_{\circ}$ to $33\%_{\circ}$for 1 day and remained far 15days, thereafter salinity was decreased from $33\%_{\circ}$ to $0\%_{\circ{$ for 1 day and remained for other 15 days. Any morphological differences of gill lamella in grey mullet were not observed in seawater and freshwater. However, on day 2 in exposure to seawater, Nile tilapia showed the edema and bloodclot in gill lamella. In the case of grey mullet, mitochondria and tubular system of chloride cell were more densely packed according to the salinity increase. The whole body of grey mullet showed no significant differences in moisture content during experimental period. However, moisture content in whole body of the Nile tilapia was signincantly decreased when exposed to seawater. Protein content in whole body of grey mullet showed no significant difference between beginning and day 15 of the experiment.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.29-29
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• 2003

In order to establish effective procedures for chromosome manipulation in Haliotis gigantea and H. discus, which are of enormous aquacultural potential, temperature-dependent measures of mitotic intervals ($\tau$$_{0}$) were determined. Mitotic intervals ($\tau$$_{0}$) in these abalone were determined by averaging the duration of the first and third embryonic divisions over a range of temperatures from 8 to 26$^{\circ}C$. The relationships of each mitotic interval at two cell ($\tau$$_{I}$), four cell ($\tau$$_{II}$ ), eight cell ($\tau$$_{III}$), sixteen cell ($\tau$$_{IV}$ ) and $\tau$$_{0}$, to temperature (T in $^{\circ}C$) in H. gigantea were log $\tau$$_{I}$ : 176.1-28.3T, log $\tau$$_{II}$ : 199.5-12.4T, log $\tau$$_{III}$ = 236.2-12.2T, log $\tau$$_{IV}$ = 269.3-14.lT and log $\tau$$_{0}$ : 83.1-32.8, respectively. The relationships of each mitotic interval at $\tau$$_{I}$, $\tau$$_{II}$ , $\tau$$_{III}$, $\tau$$_{IV}$ and $\tau$$_{0}$, to temperature in H. discus were log $\tau$$_{I}$ = 104.9-13.8T, log $\tau$$_{II}$ : 138.3-10.5T, $\tau$$_{III}$ : 172.4-10.2T, log $\tau$$_{IV}$ : 211.3-12.2T and log $\tau$$_{0}$=85.6-33.3T, respectively. There were strong, negative correlations between mitotic interval and water temperatures for all ten temperatures in these two species (H. gigantea: Y = -138.75 logX + 341.25, $R^2$ = 0.97; H. discus: Y = -112.33 logX + 255.22, $R^2$ = 0.98, where Y is mitotic interval and X is temperature).d X is temperature).rature).

• Journal of Fashion Business
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• v.13 no.6
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• pp.125-136
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• 2009

The main objective of this research was to understand the characteristics of complementary color scheme through the analysis of contemporary women's fashion color coordination as they appear in the 'Collections'. Data collection of 115 was done through review of 'pr$\hat{e}$t-$\grave{a}$-porter Collections' of four cities; Milan, London, New York, Paris. Statistical analysis of frequency and also qualitative interpretation of characteristics of complementary color harmony characteristics which appeared in four collections were completed. The main findings were as followed; (1) Only 115 complementary color schemes out of 4968 two color combinations appeared. Compared the comparative ratios of appearance frequency between the two color combination and the complementary color harmony, London showed the highest, and followed by New York, and Milan and Paris. (2)The combined color type of 'red+green' was the most frequently appeared, and followed by 'violet+yellow' and 'orange+blue'. For the type of tone harmony, the contrast tone showed the most, and followed by the similarity and identity. According to the type of complementary color combination, the type of tone harmony used differently. Some differences showed in the types of color harmony and tone harmony between collections. (3) The complementary color scheme which shown in four collections harmonized two opposite colors mainly through the strong tone contrast and this could cause tension along with interest in the image. The complementary colors intensified and brought out the attributes each other. More details, high contrast of two complementary colors of yellow and violet created a vibrant look especially when used at higher saturation. Sometimes, however, some tone variations of two hues neutralized the strong effect and sometimes enhanced each other. When they used in similarity tones or identity tones in light colors, the tension was reduced and became softened but still presented nice harmony. In the type of 'red+green' color harmony, the various color combinations were demonstrated, mostly through tone manipulation of green color. The similarity tone harmony, which used the most, could effect a better sense of harmony and present more sophisticated looks. When used in contrast tone harmony, some changes in its own color which have only one color of two the excessive intensity led a good harmony. The 'orange+blue' color harmony was shown the least and used three tone harmony almost the same ratio. In this color harmony, blue amplified its energy and brilliance of orange and seemed to work better when one color was at a lower intensity than the other. In harmony with a similarity and an identity tone, this color harmony produces a stable and calm image. (4) The complementary scheme appeared more frequently in the S/S collections than in A/W collections and showed some differences in the types of color harmony and tone harmony between seasons, however, no big differences between collections.

• Journal of Veterinary Clinics
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• v.31 no.3
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• pp.253-257
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• 2014

Five dogs presented with a history of pelvic limb lameness. On physical examination of the stifle joints, five dogs had pain, lameness, patellar luxation, or ligamentous instability. Craniocaudal and mediolateral radiographic projections revealed osteophytes or subchondral cystic lesions on the stifle joints. Based on a previously described Osteoarthrosis (OA) scoring technique, five dogs showed high OA scores. Combination of surgery and implantation of autologous adipose tissue derived mesenchymal stem cells (aAT-MSCs) or percutaneous injection of aAT-MSCs was determined with informed consent. $1{\times}10^6$ aAT-MSCs suspended in PBS was injected in the stifle joints. The follow-ups were completed 12 months after surgery. The follow-up information was based on physical examination by veterinarians. The lameness, pain on manipulation, and OA scores improved six or 12 months after implantation of aAT-MSCs. There was a radiographic evidence of decreased osteophytes and subchondral cystic lesions. These results suggest that implantation of aAT-MSCs can be considered an option for management of cases of OA in the stifle joints.

• Journal of Digital Convergence
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• v.14 no.5
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• pp.445-457
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• 2016

The purpose of the study is to provide frame work of efficient diagnostic and treatment protocol for the people with malpositioned pelvic which causes imbalance of body. Study subjects were grouped as experimental, comparison and control group. Each group consisted of five men and five women randomly assigned. Experimental group was to be tested with Thompson Terminal Technique, its corrective effect and the effect of maintaining the treatment. There were 43.01%p difference in effectiveness of the applied technique between Thompson Terminal Technique and Muscle Energy Technique. It indicates that Thompson Terminal Technique is more effective in treating pelvic misalignment than Muscle Energy Technique. As a result, the use of chiropractic and resistance exercises is proven to be effective for treating the imbalance of body. Also, to maximize the effect of treatment, it is preferable to apply Muscle Energy Technique after applying the Thompson Terminal Technique.

• Spatial Information Research
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• v.20 no.2
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• pp.1-11
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• 2012

In the current construction planning and designing process, an architectural miniature model was designed to verify the interior or exterior spatial sense of a building structure, but building of the miniature model is demand much more effort and time; in addition to this it has limitation to identify interior information of the building. For a complement of it, CAD would be used in the existing planning and designing process to visualize the building information, but its visualization is not satisfactory for the 3D volume which could be easily verified with the miniature model. CAD is the specific software for designing building structures and the 3D results are usually rendered on 2D monitor screen. Therefore, there is a shortage of cognitive immersion for the 3D space. In this paper, we introduce the conversion process of BIM shape data into the Augmented Reality contents by using a series of softwares. As a result of modification on construction plan or design we reduced the cost and time to reconstruct the final visualization. We have shown that the interior or exterior information of the building structures are easily visualized with BIM shape data on augmented reality environment. Several proposed interaction methods, such as rem oval of building components, and slice-cut operation, provide the user for the effective manipulation of models on the augmented reality environment.

• Journal of Acupuncture Research
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• v.28 no.3
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• pp.131-142
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• 2011

Objectives : The primary purpose of this study was to examine the effect of manual acupuncture at the $LI_4$, $ST_{36}$ and $LR_3$ on normal human EEG (Electroencephalogram). And the secondary purpose of this study was to analyze the relationships between the change of EEG wave form using correlation analysis. Methods : 32 channel EEG measurement was carried out in 25 healthy participants (23 males and 2 females). EEG was measured for 21 minutes including 15 minutes of retention time. In this study, power spectrum was used as a measure of complexity. Statistical analysis was performed using Spearman correlation. Results : The results were as follows; 1. In C4, O1, Fz, Pz, Cz, FCz, CP4, FC4, TP7, FT7 among 32 electrodes, ${\delta}$, $\theta$ and ${\beta}$ wave increased during 0-3 time intervals, and especially in case of TP7, FT7 ${\alpha}$ wave spontaneously decreased during 12~15 time intervals in manipulation group. 2. Between $\theta$ and ${\beta}$ wave and $\theta$ and ${\delta}$ wave, there was statistically significant positive correlation in C4, O1, Fz, Pz, Cz, FCz, CP4, FC4, TP7, FT7 according to Spearman correlation analysis. 3. Between ${\alpha}$ and ${\beta}$ wave, there was statistically significant positive correlation in TP7, FT7 according to Spearman correlation analysis. Conclusions : These results suggest that TP7 and FT7 could be typical electrodes representing change of EEG after manipulated acupuncture at the $LI_4$, $ST_{36}$ and $LR_3$. The relationships between the change of EEG wave form appear to have statistically significant positive correlation between $\theta$ and ${\beta}$ wave, $\theta$ and ${\delta}$ wave and ${\alpha}$ and ${\beta}$ wave.

• Journal of Digital Convergence
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• v.18 no.6
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• pp.451-457
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• 2020

In this study, the degree of motion sickness displayed according to actions in virtual reality is measured based on the SSQ tool, a measuring tool, and factors and symptoms affecting cyber motion sickness are investigated through comparative analysis. In the first experiment, the operation method experiment, the simple operation method is measured to be highly affected by the Nausea factor. As symptoms of this, nausea, burp and headache symptoms were developed. In the second experiment, the larger the body rotation radius, the higher the motion sickness was measured, and the greater the influence of Nausea factors. Symptoms of this were the symptoms of burping, headaches, and a full head. In the third experiment, the physical mobility experiment, motion sickness was measured highly in the non-action controller. It was measured to be greatly affected by the Nausea factor. Symptoms of this include fever, headache, and a full head. Through this study, we found that the more fixed and simple the body is operated in virtual reality, the more sensitive the user is to motion sickness, and the larger the radius of rotation, the more sensitive it is to motion sickness. This study is meaningful in identifying factors and symptoms that affect motion sickness and VR manipulation, and is expected to be used by developers in the future to recognize the degree and symptoms of motion sickness of users and to develop content.

• Korean Journal of Environment and Ecology
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• v.19 no.3
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• pp.279-286
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• 2005

This study was conducted to analyze the changing pattern of the bird community after the construction of artificial wetland at Sihwah lake from may 2000 to January 2002. Total seventy seven bird species were recorded at Sihwa artificial wetland area during the survey. Number of the bird species and individuals were increased in second year than first survey year. Especially shorebirds and raptors were more increased than other groups. Long-term and continuous monitoring of bird community would be needed to clarify the reasons of increasing pattern of bird species and individuals in artificial wetlands of Sihwa lake. Until now, this area has been considered as suitable habitat for dabbling ducks than shore birds and has simple habitat environment consisting of open water surface and reed beds. Therefore, we suggest the follows for creation of diverse habitat types: 1) seasonal water-level manipulation 2) management of diverse aquatic plants and 3) creation of diverse land cover; sandy fields, gravelly fields, grasslands, etc.