• Title/Summary/Keyword: Manipulation

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In vitro plant regeneration from axillary buds of Hibiscus syriacus L.

  • Jeon, Seo-Bum;Kang, Seung-Won;Kim, Wan-Soon;Lee, Gung-Pyo;Kim, Sun-Hyung;Seo, Sang-Gyu
    • Journal of Plant Biotechnology
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    • v.36 no.2
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    • pp.174-178
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    • 2009
  • Presently, we report a simple, reproducible and high frequency plant regeneration in Hibiscus syriacus L. using axillary buds. H. syriacus was regenerated from axillary buds directly or through a callus phase. Regenerated shoots were directly induced from young and fresh axillary buds cultured on Murashige and Skoog medium (MS) supplemented with 0.01 mg/L of the growth regulator thidiazuron (TDZ) after 2 weeks of culture. Directly induced shoots were transferred to hormone-free MS medium and root development was observed after 6 weeks. On the other hand, old and stale axillary buds were regenerated to shoots via callus induction on MS medium containing 0.01–2 mg/L TDZ after 4 weeks. A TDZ concentration of 0.01 mg/L was most effective in callus formation. Green callus was transferred to MS medium containing 0.01 mg/L α-naphthalene acetic acid (NAA) and 0.5 mg/L benzylaminopurine (BA). After 4 weeks, callus had developed into multiple shoots. Plantlets were formed from 10 week cultures of single shoots on hormone-free MS medium. Regenerated plantlets were cultured on MS medium for one month and then transferred to pots containing garden soil. Potted plants were acclimatized for one month and grown to maturity under greenhouse conditions. The present study has shown that various concentrations of plant growth regulator can be effective for in vitro plant regeneration of H. syriacus. The direct and indirect regeneration protocol presented here will be useful for understanding the manipulation and propagation of H. syriacus.

Acupuncture Stimulation for Motor Cortex Activities: Evidence from 3T Functional MRI Study

  • Choe, Bo-Young;Jeun, Sin-Soo;Kang, Sei-Kown;Park, Gi-Soon;Chung, Sung-Taek;Yoo, Seung-Schik;Chu, Myung-Ja;Lee, Hyoung-Koo;Suh, Tae-Suk
    • Proceedings of the Korean Society of Medical Physics Conference
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    • 2002.09a
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    • pp.352-355
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    • 2002
  • The purpose of this study was to investigate whether or not acupuncture of GB34 produces a significant response of the modulation of somatomotor areas by functional magnetic resonance imaging (fMRI) study. The acupoint, GB34, located in the back of the knee, is known to be effective in recovering motor function after stroke. Using 3T MRI scanner, functional MR imaging of the whole brain was performed in 12 normal healthy subjects during two stimulation paradigms; acupuncture manipulation on GB 34 and sham points. This study investigates the activation of the mortor cortex elicited by a soft and an intensified stimulation of GB 34. Three different paradigms were carried out to detect any possible modulation of the Blood Oxygenation Level Dependent (BOLD) response in the somatomortor area to motor stimulation through acupuncture. Group analysis from seven individuals showed that bilateral sensorimotor areas (BA 3,4,6 and 7) showed stimulation related BOLD signal contrast of approximately 6% whereas very few areas were activated when sham stimulation is given. The present study shows that acupuncture fMRI study can be safely conducted in 3T MRI environment, and acupuncture stimulation in GB34 modulates the cortical activities of the somatomotor area in human. The present findings may shed light on the CNS mechanism of motor function by acupuncture and form a basis for future investigations of motor modulation circuits in the stroke patients.

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Protoplast-Mediated Transformation of the Filamentous Fungus Cladosporium phlei: Evidence of Tandem Repeats of the Integrative Transforming Vector

  • Kim, Jung-Ae;Kim, Jung-Mi;Kim, Hwan-Gyu;Kim, Beom-Tae;Hwang, Ki-Jun;Park, Seung-Moon;Yang, Moon-Sik;Kim, Dae-Hyuk
    • The Plant Pathology Journal
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    • v.25 no.2
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    • pp.179-183
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    • 2009
  • To facilitate the genetic manipulation of Cladosporium phlei, a causal agent of leaf spot disease in timothy (Phleum pretense), protoplast-mediated transformation of C. phlei has been developed and the resulting transformants were characterized in this study. Hygromycin B resistance was applied as a dominant selection marker due to the sensitivity of C. phlei to this antibiotic. The transformation efficiency ranged from approximately 20-100 transformants per experiment. Southern blot analysis of stable transformants revealed that transformation occurred by way of stable integration of the vector DNA into the fungal chromosome. PCR analysis and plasmid rescuing of randomly selected transformants suggested that integration of tandem repeat copies of vector DNA was common. In addition, multiple integrations of the transforming vector at different chromosomal sites were also observed. The establishment of a transformation method for C. phlei facilitates strain improvement of this fungus and can be applied as an initial step in the molecular analysis of pigment production in this fungus.

Modified Submental Intubation in Panfacial Bone Fracture Patients (전안면골 골절에서의 변형된 아래턱밑 삽관)

  • Choi, Sang-Mun;Song, Seung-Han;Kang, Nak-Heon
    • Archives of Plastic Surgery
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    • v.38 no.1
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    • pp.127-129
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    • 2011
  • Purpose: Nasotracheal or oral intubation procedure is widely used for facial bone fractures. However, during the operation intubated tube can interfere or obstruct the view of the operator. We authors used a modified submental intubation method in panfacial bone fracture patients for intact airway and the operation view. Methods: After intravenous induction of anaesthesia, traditional orotracheal tubation was done. A horizontal incision was made 2 cm from the midline, 2 cm medial to and parallel with the mandible in the submental region. 1 In order to approach to the floor of the oral cavity, a haemostat was pushed through the soft tissues. A chest tube front cover was applied to the intubation tube and the tube was inserted through the submental tunnel. Orotracheal tube was disconnected and pulled back through the soft tissue and secured with a suture. Results: The procedure took about 30 minutes and there were no problems during the intubation. Intraoral manipulation and occlusal checks were free without any interference. Extubation was also easily done without any complications such as lung aspiration, infection, hematoma, or fistula. Conclusion: Submental endotracheal intubation is fast, safe, easy to use and free from the concern about the tube being pull back again. Conventional submental intubations are being held without any coverage of the tip. We authors applied the modified method to the trauma patients and obtained satisfactory results. From the above advantages, modified submental intubation can be widely available not only in fractured patients, but also in aesthetic or orthognathic surgeries.

Enhancement of Image Sharpness in X-ray Digital Tomosynthesis Using Self-Layer Subtraction Backprojection Method (관심 단층 제거 후 역투사법을 이용한 X-선 디지털 영상합성법에서의 단층영상 선명도 향상에 관한 연구)

  • Shon, Cheol-Soon;Cho, Min-Kook;Lim, Chang-Hwy;Cheong, Min-Ho;Kim, Ho-Kyung;Lee, Sung-Sik
    • Journal of the Korean Society for Nondestructive Testing
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    • v.27 no.1
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    • pp.8-14
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    • 2007
  • X-ray digital tomosynthesis is widely used in the nondestructive testing and evaluation, especially for the printed circuit boards (PCBs). In this study, we propose a simple method to reduce the blur artefact, frequently claimed in the conventional digital tomosynthesis based on SAA (shift-and-add) algorithm, and thus restore the image sharpness. The proposed method is basically based on the SAA, but has a correction procedure by finding blur artefacts from the forward-and back-projection for the firstly obtained, manipulated backprojection data. The manipulation is the replacement of the original data at the POI (plane-of-interest) by zeros. This method has been compared with the conventional SAA algorithm using the experimental measurements and Monte Carlo simulation for the designed PCB phantom. The comparison showed a much enhancement of sharpness in the images obtained from the proposed method.

Outcome Measurement and Degree of Economic Contribution for the National R&D Reports (국가 R&D 보고서 원문의 활용성과 및 경제적 기여도 분석)

  • Kwak, Seung-Jin;Choi, Jae-Hwang;Kim, Jeong-Taek;Park, Yong-Jae;Choi, Kiseok
    • Journal of the Korean Society for Library and Information Science
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    • v.49 no.1
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    • pp.113-128
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    • 2015
  • As the major developed countries of the world have realized the importance of common information and its utilization, they have begun to seek a plan that will facilitate manipulation of common information. Despite development of the National R&D activities and its quantitative increase in Korea, its practical use still remains at a lower level. Therefore, it is worthwhile to analyze practical use and economic contribution in order to achieve higher profits. There are two purposes of this study. The first purpose is to draw a conclusion by analyzing the practical uses of the National R&D Reports, and the second purpose is to evaluate economic contributions by the National R&D Reports. In order to achieve the objectives of this study, literature review, survey, focus group interview, and experts' consultation were conducted as a way to deepen research, simultaneously comprising the quantitative and qualitative methods.

An Energy-Efficient Clustering Design Apply Security Method in Ubiquitous Sensor Networks (USN에서 보안을 적용한 에너지 효율적 클러스터링 설계)

  • Nam, Do-Hyun;Min, Hong-Ki
    • Journal of IKEEE
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    • v.11 no.4
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    • pp.205-212
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    • 2007
  • The ubiquitous sensor network consists of micro sensors with wireless communication capabilities. Compared to wired communication, wireless communication is more subject to eavesdropping as well as data variation and manipulation. Accordingly, there must be efforts to secure the information delivered over the sensor network. Providing security to the sensor network, however, requires additional energy consumption, which is an important issue since energy transformation is difficult to implement in a sensor network. This paper proposes a routing mechanism based on the energy-efficient cluster that features security functions capable of safely processing the data acquired from the sensor network. The proposed algorithm reduces energy consumption by fixing the clusters formed at the initial stage and using the pre-distribution scheme so that the cluster and node keys generated and exchanged at the initial stage are not re-generated or re-exchanged. Simulation experiments confirmed that the proposed approach reduces energy consumption compared to implementing security measures to the conventional cluster-based routing mechanism.

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Aesthetic Strategies in Steina and Woody Vasulka's Video Art (비디오아티스트 슈테이너 바술카와 우디 바술카의 미적 전략)

  • Lim, Shan
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.3
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    • pp.261-266
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    • 2020
  • As pioneers of the early video art, Steina Vasulka(1940-) and Woody Vasulka(1937-2019) had lead not only their own experimental arts, but also entire changes of contemporary avant-garde performance, music, and visual art. Two artists invented and developed electronic machines for video image-processing by collaborating with engineers, and performed creative experiment on transformation of digital image. For them, video art is not just a means of documentation. The Vasulkas' artistic practices were not bounded by conventional canons and rules in art world, and preferably were parts of active aesthetic strategies for coexistence of vision of human and vision of machine. Particularly, their video art recognized the video as the key medium in an era where media technology began to dominate the system of communication, and established artist's authority over manipulation of moving image electronically without depending on video camera. In that regard, we can value on their video art. Therefore, the paper reflects on the Vasulkas' art and life which have not yet been studied, and suggests academic interests in the context of their artistic activities and aesthetic strategies.

Managing Scheme for 3-dimensional Geo-features using XML

  • Kim, Kyong-Ho;Choe, Seung-Keol;Lee, Jong-Hun;Yang, Young-Kyu
    • Proceedings of the Korean Association of Geographic Inforamtion Studies Conference
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    • 1999.12a
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    • pp.47-51
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    • 1999
  • Geo-features play a key role in object-oriented or feature-based geo-processing system. So the strategy for how-to-model and how-to-manage the geo-features builds the main architecture of the entire system and also supports the efficiency and functionality of the system. Unlike the conventional 2D geo-processing system, geo-features in 3D GIS have lots to be considered to model regarding the efficient manipulation and analysis and visualization. When the system is running on the Web, it should also be considered that how to leverage the level of detail and the level of automation of modeling in addition to the support for client side data interoperability. We built a set of 3D geo-features, and each geo-feature contains a set of aspatial data and 3D geo-primitives. The 3D geo-primitives contain the fundamental modeling data such as the height of building and the burial depth of gas pipeline. We separated the additional modeling data on the geometry and appearance of the model from the fundamental modeling data to make the table in database more concise and to allow the users more freedom to represent the geo-object. To get the users to build and exchange their own data, we devised a fie format called VGFF 2.0 which stands for Virtual GIS File Format. It is to describe the three dimensional geo-information in XML(extensible Markup Language). The DTD(Document Type Definition) of VGFF 2.0 is parsed using the DOM(Document Object Model). We also developed the authoring tools for users can make their own 3D geo-features and model and save the data to VGFF 2.0 format. We are now expecting the VGFF 2.0 evolve to the 3D version of SVG(Scalable Vector Graphics) especially for 3D GIS on the Web.

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Technological Trend of Endoscopic Robots (내시경 로봇의 기술동향)

  • Kim, Min Young;Cho, Hyungsuck
    • Journal of Institute of Control, Robotics and Systems
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    • v.20 no.3
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    • pp.345-355
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    • 2014
  • Since the beginning of the 21st century, emergence of innovative technologies in robotic and telepresence surgery has revolutionized minimally access surgery and continually has advanced them till recent years. One of such surgeries is endoscopic surgery, in which endoscope and endoscopic instruments are inserted into the body through small incision or natural openings, surgical operations being carried out by a laparoscopic procedure. Due to a vast amount of developments in this technology, this review article describes only a technological state-of-the arts and trend of endoscopic robots, being further limited to the aspects of key components, their functional requirements and operational procedure in surgery. In particular, it first describes technological limitations in developments of key components and then focuses on the description of the performance required for their functions, which include position control, tracking, navigation, and manipulation of the flexible endoscope body and its end effector as well, and so on. In spite of these rapid developments in functional components, endoscopic surgical robots should be much smaller, less expensive, easier to operate, and should seamlessly integrate emerging technologies for their intelligent vision and dexterous hands not only from the points of the view of surgical, ergonomic but also from safety. We believe that in these respects a medical robotic technology related to endoscopic surgery continues to be revolutionized in the near future, sufficient enough to replace almost all kinds of current endoscopic surgery. This issue remains to be addressed elsewhere in some other review articles.