• 한국환경과학회지
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• 제30권1호
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• pp.29-43
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• 2021

This study analyzed the status of climate-change indicator plants native to the main islands of the Korean peninsula, while elucidating their distribution characteristics. Information on flora from over 129 island locations, comprising more than 100 species of native plants, was collected, compiled into a database, and utilized as raw data. The distribution of 193 climate-change indicator plants was confirmed. The distribution area of broadleaf evergreen trees and ferns, including Mallotus japonicus and Cyrtomium falcatum, was relatively wide. In contrast, the distribution of common northern plants such as Corydalis turtschaninovii and Malus baccata was limited. If global warming persists, northern plant distribution is expected to decrease rapidly in the Korean Peninsula island region, while the northern limit line of the southern plants is expected to migrate further northward. During this process, it is likely that the plant congregation structure and species diversity within the island region will change dynamically. In this study, comparative analyses between species and regions were conducted by assessing the relative frequency of their occurrence, and six types of botanical geographic distribution patterns were noted.

• 생명과학회지
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• 제22권6호
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• pp.788-793
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• 2012

본 연구는 어류 병원균의 비 유전성을 가지는 항생제 내성균인 persister cell에 관한 연구이다. Persister cell은 기존의 항생제를 분해하는 저항균체(resistant cell)와는 다른 특성으로 항생제에 대한 저항성을 가지는데, 항생제가 존재하는 환경에서 새로운 기작으로 항생제에 대한 내성을 형성한다. 그래서 기존의 양식장에서 어류를 키울 때 어류에 투여하는 항생제는 일반적인 균주의 사멸 항생제 농도보다 더 높은 농도의 항생제를 투여하게 된다. 특히, E. tarda에 대한 다양한 항생제가 개발되어 있지만 내성균의 출현으로 그 효과가 좋지 않으며, 또한 persister cell에 의한 질병 재발을 방지하기 위해 균사멸 농도보다 훨씬 높은 농도의 항생제를 처리하고 있다. Persiser cell의 특이적인 저감 효과를 확인 하기 위하여 선별된 3종의 식물 추출물(돌외, 예덕나무, 상산)을 항생제와 함께 사용하였으며, 돌외와 예덕나무가 100 ${\mu}g/ml$, 상산은 200 ${\mu}g/ml$의 농도에서 persister cell의 사멸 효과를 나타내었다. 또한 넙치를 이용한 12일간의 누적 폐사율을 조사한 결과, 식물 추출물과 항생제 혼합액의 복강 투여구가 항생제 단독의 복강 투여구 보다 낮은 누적 폐사율이 관찰되었고, 항생제에 첨가한 식물 추출물의 농도가 돌외 30 ${\mu}g/ml$, 예덕나무 10 ${\mu}g/ml$, 상산 10 ${\mu}g/ml$의 농도 투여구에서 가장 낮은 누적 폐사율을 나타내었다. 따라서 본 연구에 사용된 세가지 추출물들(돌외, 예덕나무, 상산)은 항균 활성을 가지지 않은 농도에서 항생제와 병용하여 persister cell의 저감 효과가 있음을 확인하였다.

• 농업생명과학연구
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• 제51권4호
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• pp.97-109
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• 2017

본 시험은 항염에 효과가 있다고 알려진 식물 추출물의 첨가가 반추위 발효와 메탄 생성에 미치는 영향을 알아보기 위해 in vitro 실험을 수행하였다. 항염과 항산화 효과가 있는 산뽕나무, 뽕나무, 예덕나무, 오동나무, 방아풀과 은행나무 6종을 선발하였다. 반추위액과 McDougall buffer 혼합액 15mL과 티모시 0.3g, 각각의 추출물을 기질의 5%로 넣고 $39^{\circ}C$에서 3, 9, 12, 24, 48 그리고 72시간 배양하였다. 항염 효과가 있는 식물 추출물의 첨가는 반추위 발효 성상(pH, 건물소화율, Glucose 농도, 암모니아 농도, 단백질 농도, 미생물 성장량, 휘발성지방산)에는 영향을 미치지 않았다. 총 가스 발생량은 각기 다른 양상을 보였으며, 이산화탄소 발생량은 48시간대에서 예덕나무와 방아풀에서 대조구에 비해 유의적(p<0.05)으로 높았다. 또한 메탄 발생량은 배양 초기에는 대조구보다 첨가구에서 유의적(p<0.05)으로 감소하였으나, 발효가 진행될수록 대조구에 비해 첨가구에서 더 많은 메탄이 발생하였다. Polyphenol과 flavonoid는 은행나무 추출물구에서 가장 높았다. 본 시험의 결과에서 항염에 효과가 있는 식물 추출물을 in vitro 반추위 배양액에 첨가하였을 때, 반추위 발효에는 영향을 미치지 않았고, 메탄은 초기 발효에 저감 효과가 있는 것으로 사료된다.

• 한국환경과학회지
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• 제23권3호
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• pp.335-357
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• 2014

This study aims at classifying and interpreting on the vegetation structure and the vegetation transition over 25 years (between 1986 and 2010), and the correlation with the change of some conditions (the vegetation height and coverage on each layer and the climate factors as WI, CI, mean annual temperature, mean annual total precipitation etc.) in the Evergreen Broad-Leaved Forest,, Hong-Do island. The EBLF is classified into five units of vegetation (Hedera rhombea-Machilus thunbergii community (M-M comm.), Castanopsis sieboldii forest (Machilus japonica-Castanopsis sieboldii community; Raphiolepis indica var. umbellata-C. sieboldii community), community (Qa comm.), Carpinus turczaninovii community (Ct comm.), Camellia japonica stand (Cj stand)). The vegetation transition by CCA had high correlation with the height and coverage on each layer and the climate factors, and it did the succession (transition) that the M-M comm. (2010) from Mallotus japonicus community Machilus thunbergii community Carpinus coreana community (Cc comm.) Aucuba japonica community (Aj comm.) Trachelos permum asiaticum var. intermedium-Quercus acuta community (TQ comm.) (1986), the communities of C. sieboldii forest (2010) from Aj comm. TQ comm. Raphiolepis umbellata-Camellia japonica community (RC comm.) (1986), the Qa comm. (2010) from Ardisia japonica-Castanopsis sieboldii community (AC comm.) and TQ comm. (1986), the Ct comm. (2010) from Cc comm. RC comm. Aj comm. Quercus serrata community and the Cj stand (2010) from AC comm. (1986). the height and coverage on each layer are also changed.

• 생약학회지
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• 제39권4호
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• pp.290-294
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• 2008

The objective of this study is screening the anti-oxidative effects of several plant MeOH extracts against oxidative stress in Neuroblastoma cell. Oxidative stress has been implicated in the pathogenesis of many neurotoxicity, neurodegenerative disorders and cell death. This oxidative stress is generated by ROS (Reactive Oxygen Species) such as nitric oxide, nitrogen dioxide, peroxyl, superoxide ($O_2^-$), hydroxyl, alkoxyl. So, in the present study, we induced oxidative stress by treatment of sodium nitroprusside (2.5 mM) in human neuroblastoma SH-SY5Y cell which was treated samples before 24hr, and cell viability was measured by MTT reduction assay. Of those tested, the extracts of Paeonia japonica (roots), Eucommia ulmoides (炒)(barks), Paeonia japonica (曝乾)(roots), Phyllostachys bambusoides (stems), Polygala tenuifolia (去心, 炒)(roots), Paeonia japonica (roots), Polygala tenuifolia (roots), Machilus thunbergii (barks), Mallotus japonicus (leaves), Poria cocos (whole), Sophora flavescens (roots), Angelica tenuissima (roots), Angelica gigas (當歸尾)(roots) showed anti-oxidative effects[$EC_{50}$<15.20 ${\mu}g$/ml(Carnosine:Positive control)]in dose dependent manner.

• Journal of Forest and Environmental Science
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• 제32권2호
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• pp.196-204
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• 2016

In this study, vegetation succession and the rate of consequent topsoil development were investigated in shallow landslide scars of sedimentary rock slopes covered by volcanic ashes and pumice in Kagoshima prefecture, Japan. Seven shallow landslide scars of different ages were selected as study areas. In the initial period after the occurrence of a shallow landslide, deciduous broad-leaved trees such as Mallotus japonicus or Callicarpa mollis were occupied in the areas. Approximately 30 years after the landslide, evergreen broad-leaved trees such as Cinnamomum japonicum invaded in the areas, already existed present deciduous broad-leaved trees. After 50 years, the summit of the canopy comprised evergreen broad-leaved trees such as Castanopsis cuspidata var. sieboldii and Machilus thunbergii. Moreover, the diversity of vegetation invading the site reached the maximum after 15 years, followed by a decrease and stability in the number of trees. The total basal areas under vegetation increased with time. It was concluded that the vegetation community reaches the climax stage approximately 50 years after the occurrence of a shallow landslide in the study areas, in terms of the Fisher-Williams index of diversity (${\alpha}$) and the prevalence of evergreen broad-leaved trees. Moreover, according to the results of topsoil measurement in the study areas, the topsoil was formed at the rate of 0.31 cm/year. The development of topsoil usually functions to improve the multi-faceted functions of a forest. However, when the increased depth of topsoil exceeds the stability threshold, the conditions for a shallow landslide occurrence are satisfied. Therefore, we indicated to control the depth of topsoil and strengthen its resistance by forest management in order to restrain the occurrence of shallow landslides.

• 환경영향평가
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• 제20권2호
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• pp.151-162
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• 2011

The purpose of this study is suggest to restoration model of Pinus thunbergii in Saha-gu, Busan Metropolitan City. The result of this study is summarized as follows; As the results of this study, vegetation restoration model is presented by separating community planting and edge planting. The community planting species of tree layer were Pinus thunbergii and Quercus acutissima and Quercus dentata and Quercus serrata and Quercus alienna and Quercus variabilis. The community planting species of subtree layer were Platycarya strobilacea and Prunus sargentii and Styrax japonica and Eurya japonica and Morus bombycis. The community planting species of shrub layer are Ulmus pavifolia and Ulmus davidiana and Lindera obtusiloba and Elaeagnus macrophylla and Mallotus japonicus and Ligustrum obtusifolium and Sorbus alnifolia and Rhus trichocarpa and Zanthoxylum schinifolium and Rosa wichuraiana and Rhus chinensis and Viburnum erosum and Rhododendron mucronulatum and Rhododendron yedoense and Indigofera pseudotinctoria. And the planting species of edge vegetation are Japanese Angelica and Symplocos chinensis and Pittosporum tobira and Lespedeza maximowiczii and Lespedeza bicolor and Rubus coreanus and Rubus idaeus and Vitis thunbergii and Ampelopsis brevipedunculata and Rosa multiflora. Considering the population of individuals up to layers in each $400m^2$ area, it was composed of 24 in tree layer, 35 in subtree layer, 410 in shrub layer and 34% herb layer in the Pinus thunbergii community. And the average of breast-high area and canopy area was $10,852cm^2$ in tree layer, in subtree layer $1,546cm^2$, in shrub layer $1,158,660cm^2$. The shortest distance between trees was calculated as 2.0m in tree layer, 1.9m in subtree layer.

• 한국환경복원기술학회지
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• 제13권2호
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• pp.80-94
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• 2010

The purpose of this study is suggest to restoration model of Pinus thunbergii in Saha-gu, Busan. The result of this study is summarized as follows. As the results of this study, vegetation restoration model is presented by separating community planting and edge planting. In community planting, as a group of canopy, there are 6 species; Pinus thunbergii, Quercus acutissima, Quercus dentata, Quercus serrata, Quercus alienna, Quercus variabilis. As a group of understory, there are 5 species; Platycarya strobilacea, Prunus sargentii, Styrax japonica, Eurya japonica, Morus bombycis. Also as a group of shrub, there were 15 kinds of species; Ulmus pavifolia, Ulmus davidiana, Lindera obtusiloba, Elaeagnus macrophylla, Mallotus japonicus, Ligustrum obtusifolium, Sorbus alnifolia, Rhus trichocarpa, Zanthoxylum schinifolium, Rosa wichuraiana, Rhus chinensis, Viburnum erosum, Rhododendron mucronulatum, Rhododendron yedoense, Indigofera pseudotinctoria. And as a group of edge vegetation, there were 10 kinds of species; Japanese Angelica, Symplocos chinensis, Pittosporum tobira, Lespedeza maximowiczii, Lespedeza bicolor, Rubus coreanus, Rubus idaeus, Vitis thunbergii, Ampelopsis brevipedunculata, Rosa multiflora. Vegetation restoration models of Pinus thunbergii community were calculated the units $400m^2$ for the average populations of the woody layer is 24 in canopy layer, 35 in understory layer, 410 in shrub layer, 34% herbaceous layer ground cover. And the average of breast-high area and canopy area is $10,852cm^2$ in canopy layer, in understory layer $1,546cm^2$, in shrub layer $1,158,660cm^2$. The shortest distance between trees is calculated as 2.0m in canopy layer, 1.9m in understory layer.

• Archives of Pharmacal Research
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• 제24권2호
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• pp.114-118
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• 2001

The present study was undertaken to investigate whether or not the hepatoprotective activity of acetylbergenin was superior to bergenin in carbon tetrachloride ($CCl_4$)-intoxicated rat. Acetylbergenin was synthesized by acetylating bergenin, which was isolated from Mallotus japonicus. The hepatoprotective effects of acetylbergenin were examined against $CCl_4$-induced liver damage in rats by means of serum and liver biochemical Indices. Acetylbergenin was administered orally once daily for 7 successive days, then a 0.5 ${m/kg}$ mixture of $CCl_4$in olive oil (1:1) was intraperitoneally injected at 12 h and 36 h after the final administration of acetylbergenin. Pretreatment with acetylbergenin reduced the elevated serum enzymatic activities of alanine/aspartate aminotransferase, sorbitol dehydrogenase and $\gamma$-glutamyltransferase in a dose dependent fashion. Acetylbergenin also prevented the elevation of hepatic malondialdehyde formation and depletion of glutathione content dose dependently in $CCl_4$-intoxicates rats. In addition, the decreased activities of glutathione S-transferase and glutathione reductase were restored to almost normal levels. The results of this study strongly suggest that acetylbergenin n has potent hepatoprotective activity against $CCl_4$-induced hepatic damage in rats by glutathione-mediated detoxification as well as having free radical scavenging activity. In addition, acetylbergenin doses of 50 ${mg/kg}$showed almost the same levels of hepatoprotection activity as 100 ${mg/kg}$ of bergenin, indicating that lipophilic acetylbergenin is more active against the antihepatotoxic effects of $CCl_4$ than those of the much less lipophilic bergenin.

• 한국자원식물학회지
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• 제20권2호
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• pp.113-124
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• 2007

본 연구는 금강하류 철새조망대 일대의 해안식물과 내륙에 분포하는 식물 및 경작지 주변의 관속식물을 조사한 후 식물구계학적 특정식물종과 귀화식물 등을 조사하여 다음과 같은 결론을 도출하였다. 본 대상지인 금강하류 일대의 관속식물상을 조사한 결과, 74과 179속 211종 24변종 3품종으로 총 238종류가 확인되었다. 이중 목본식물은 총 238종류 중 60종류(25.2%), 초본식물은 178종류(74.8%)로 분석되었다. 또한, 양치식물은 4과 4속 4종류, 나자식물은 2과 2속 3종류, 피자식물은 68과 173속 231종류가 확인되었다. 한국특산식물에는 은사시나무와 개나리가 확인되었으며, 식물구계학적 특정식물종에는 III등급에 참오굴잎버들, I등급에는 물오리나무, 굴참나무, 예덕나무, 개질경이, 뚜껑덩굴, 보풀 등의 6종류가 확인되었다. 귀화식물은 14과 35속 40종 2변종으로 총 42종류와 귀화율은 17.6%로 분석되었다. 귀화식물 중 생태계교란야생식물인 돼지풀(Ambrosia artemisiifolia var. elatior)은 꽃가루 알레르기로 인한 인체에 피해를 주기 때문에 지속적인 관리와 대책이 요망된다.