Browse > Article

Anti-oxidative Effect of Some Plant Extracts Against Nitric Oxide-induced Oxidative Stress on Neuronal Cell  

Koo, Uk (Natural Product Research Institute, College of Pharmacy, Seoul National University)
Lee, Hak-Ju (Korea Forest Research Institute)
Lee, Dong-Ho (College of Life sciences & Biotechnology, Korea University)
Lee, Hyun-Jung (Korea Forest Research Institute)
Ham, Ah-Rom (Natural Product Research Institute, College of Pharmacy, Seoul National University)
Cho, Eun-Young (Natural Product Research Institute, College of Pharmacy, Seoul National University)
Mar, Woong-Chon (Natural Product Research Institute, College of Pharmacy, Seoul National University)
Publication Information
Korean Journal of Pharmacognosy / v.39, no.4, 2008 , pp. 290-294 More about this Journal
Abstract
The objective of this study is screening the anti-oxidative effects of several plant MeOH extracts against oxidative stress in Neuroblastoma cell. Oxidative stress has been implicated in the pathogenesis of many neurotoxicity, neurodegenerative disorders and cell death. This oxidative stress is generated by ROS (Reactive Oxygen Species) such as nitric oxide, nitrogen dioxide, peroxyl, superoxide ($O_2^-$), hydroxyl, alkoxyl. So, in the present study, we induced oxidative stress by treatment of sodium nitroprusside (2.5 mM) in human neuroblastoma SH-SY5Y cell which was treated samples before 24hr, and cell viability was measured by MTT reduction assay. Of those tested, the extracts of Paeonia japonica (roots), Eucommia ulmoides (炒)(barks), Paeonia japonica (曝乾)(roots), Phyllostachys bambusoides (stems), Polygala tenuifolia (去心, 炒)(roots), Paeonia japonica (roots), Polygala tenuifolia (roots), Machilus thunbergii (barks), Mallotus japonicus (leaves), Poria cocos (whole), Sophora flavescens (roots), Angelica tenuissima (roots), Angelica gigas (當歸尾)(roots) showed anti-oxidative effects[$EC_{50}$<15.20 ${\mu}g$/ml(Carnosine:Positive control)]in dose dependent manner.
Keywords
Sodium nitroprusside; Oxidative stress; Anti-oxidative effects; MTT reduction assay; $EC_{50}$;
Citations & Related Records

Times Cited By SCOPUS : 1
연도 인용수 순위
1 Chasovnikova, L. V., Formazyuk, V. E., Sergienko, V. I., Boldyrev, A. A. and Severin, S. E. (1990) The antioxidative properties of carnosine and other drugs. Biochem. Int. 20: 1097-1103
2 Veretianov II. (1956) Treatment of hypertension with tincture of Cortex Eucommiae. Voen. Med. Zh. 12: 57-58
3 Mohammad, Y. M., Divani, A. A., Kirmani, J. F., Harris-Lane, P., Qureshi, A. I. (2004) Acute treatment for ischemic stroke in 2004. Emerg Radiol. 11: 83-86   DOI
4 Reeve, A. K., Krishnan, K. J. and Turnbull, D. M. (2008) Age related mitochondrial degenerative disorders in humans. Biotechnol. J. 3: 750-756   DOI   ScienceOn
5 Boldyrev, A. A., Dupin, A.M., Bunin, A. Y., Babizhaev, M. A. and Severin, S. E. (1987) The antioxidative properties of carnosine, a natural histidine containing dipeptide. Biochem. Int. 15: 1105-1113
6 Solov'eva, E. I., Mironova, O. P., Baranova, O. A., Bekman, E. M., Asechev, A. V., Fedin, A. I. and Azizova, O. A. (2008) The free-radical processes and antioxidant therapy in brain ischemia. Zh. Nevrol. Psikhiatr. Im. S S Korsakova. 108: 37-42
7 Castillo, J., Rama, R. and Davalos, A. (2000) Nitric oxiderelated brain damage in acute ischemic stroke. Stroke 31: 852-857   DOI   ScienceOn
8 Puddu, P., Puddu, G. M., Cravero, E., Rosati, M. and Muscari, A. (2008) The molecular sources of reactive oxygen species in hypertension. Blood Press 17: 70-77   DOI   ScienceOn
9 Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M. and Telser, J. (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 39: 44-84   DOI   ScienceOn
10 Calabrese, V., Colombrita, C., Guagliano, E., Sapienza, M., Ravagna, A., Cardile, V., Scapagnini, G., Santoro, A. M., Mangiameli, A., Butterfield, D. A., Giuffrida Stella, A. M. and Rizzarelli, E. (2005) Protective effect of carnosine during nitrosative stress in astroglial cell cultures. Neurochem. Res. 30: 797-807   DOI
11 Chang, G. T., Min, S. Y., Kim, J. H., Kim, S. H., Kim, J. K. and Kim, C. H. (2005) Anti-thrombic activity of Korean herbal medicine, Dae-Jo-Whan and its herbs. Vascul. Pharmacol. 43: 283-288   DOI   ScienceOn
12 Knott, A. B., Perkins, G., Schwarzenbacher, R. and Bossy-Wetzel, E. (2008) Mitochondrial fragmentation in neurodegeneration. Nat. Rev. Neurosci. 9: 505-518   DOI   ScienceOn