• Title/Summary/Keyword: Major persistence

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Livestock-associated methicillin-resistant Staphylococcus aureus in Korea: antimicrobial resistance and molecular characteristics of LA-MRSA strains isolated from pigs, pig farmers, and farm environment

  • Back, Seung Hyun;Eom, Hong Sik;Lee, Haeng Ho;Lee, Gi Yong;Park, Kun Taek;Yang, Soo-Jin
    • Journal of Veterinary Science
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    • v.21 no.1
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    • pp.2.1-2.14
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    • 2020
  • The emergence of livestock-associated (LA)-methicillin-resistant Staphylococcus aureus (MRSA) in livestock animal has become a significant zoonotic concern. In the present study, we investigated nationwide prevalence of LA-MRSA across pork production chain including pig farms, slaughterhouses, and retail markets. A total of 40 MRSA strains were isolated during the investigation and the overall prevalence of MRSA was 3.4% (n = 37), 0.6% (n = 2), and 0.4% (n = 1) in pig farms, slaughterhouses, and retail markets, respectively. Multilocus sequence typing analyses revealed that the 2 most significant clonal lineages in pork production chain in Korea were ST398 (n = 25) and ST541 (n = 6). All of the 40 MRSA isolates were further characterized to investigate key genotypic and phenotypic correlates associated with the emergence and spread of clonal complex 398 (CC398; ST398, and ST541) LA-MRSA. Although the prevalence of swine-associated MRSA was still relatively low and mostly restricted to pig farms, multidrug-resistant CC398 LA-MRSA isolates with new spa types (t18102 and t18103) were identified as a major clonal lineage. The CC398 LA-MRSA strains tended to exhibit increased levels of multiple drug resistance (MDR) phenotype compared with non-CC398 MRSA strains. Of note, in comparison with non-CC398 MRSA isolates, CC398 LA-MRSA isolates exhibited significantly enhanced tetracycline (TET) and zinc resistance. These findings suggested that co-selection pressure associated with MDR phenotype, especially TET resistance, and zinc resistance may have played a significant role in the emergence and persistence of CC398 LA-MRSA in pig farms in Korea.

Effect of Activated Carbon, Orpar or Zeolite on Leaching Loss of Fenitrothion, Triadimefon and Diniconazole in Model Green of Golf Course (골프장 모형그린에서 활성탄, Orpar또는 Zeolite의 처리가 Fenitrothion, Triadimefon, Diniconazole의 용탈에 미치는 영향)

  • Oh, Sang-Sil;Koh, Yong-Ku;Chung, Jong-Bae;Hyun, Hae-Nam
    • Applied Biological Chemistry
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    • v.44 no.2
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    • pp.97-102
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    • 2001
  • Cheju island depends on a hydrogeologically vulnerable aquifer system as its principle source of drinking water. Most of golf courses are located in the area which is important for the ground water recharge, and pesticides are applied to golf courses often at relatively high rates. Therefore, turf pesticides in golf course should be applied without adversely impacting ground water. In this experiment, downward movement of pesticides was monitored in model greens of golf course, where different adsorbents were layered in 3-cm thickness at 35-cm depth, and effect of the adsorption layer on the leaching loss of pesticides was investigated. Major leachings were observed in the periods of heavy rain and very limited leaching was observed under artificial irrigation. Fenitrothion and triadimefon, which have relatively short persistence and high adsorption coefficient, were found in the leachate in low concentrations only at the first rainfall event, around 20 days after the pesticide application. However, diniconazole, which has a relatively long half-life (97 days), was detected in the leachate during the whole period of experiment and concentration was much higher than those of the other pesticides. Maximum leachate concentrations were 1.9, 10.3, and 84.5 ${\mu}l^{-1}$ for fenitrothion, triadimefon, and diniconazole, respectively. Therefore, in golf course green which allows rapid water percolation and has extremely low adsorption capacity, persistence in soil could be more important factor in determination of leaching potential of pesticides. Total quantity of pesticides leached from the model green was <0.2% for fenitrothion and triadimefon and 1.8% for diniconazole. Adsorption layers significantly reduced pesticide leaching, and active carbon and Orpar were more effective than zeolite. In the model green having adsorption layer of active carbon or Orpar, leaching loss of pesticides was reduced below 0.01% of the initial application.

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Studies on Degradation of Butachlor and Nitrofen in Different Soil Conditions (토양중(土壤中) Butachlor 와 Nitrofen의 분해(分解)에 관(關)한 연구(硏究))

  • Oh, Byung-Youl;Jeong, Young-Ho;Lee, Byung-Moo
    • Applied Biological Chemistry
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    • v.24 no.2
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    • pp.112-119
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    • 1981
  • The persistence of preemergence herbicides, butachlor [2-chloro-2,6-diethyl-N(butoxymethyl) acetanilide] and nitrofen(2,4-dichlorophenyl-4-nitrophenyl ether), at 10 ppm level in different soil conditions amended with organic matter and lime was studied under flooded and field moisture capacity. The microbial breakdown played a major role in the dissipation of the herbicides in soil. Nitrofen degradation in flooded soil was greatly accelerated, while it was slowed down in field moisture capacity as compared with butachlor. Increased amendment of rice straw to the soil shortened the half-life of butachlor under flooded condition, however it prolonged that of butachlor when the amendment was exceeded over 1% on dry weight basis. Liming the soil stimulated decomposition of the herbicides in the soil systems, which ap pears to be pH independent.

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춘천-홍천 지역 용두리 편마암 복합체내에 산출하는 남정석: 변성지구조적 의의

  • 조문섭;김종욱
    • The Journal of the Petrological Society of Korea
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    • v.2 no.1
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    • pp.1-8
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    • 1993
  • We report on kyanite newly found in the Yongduri gneiss complex of the Chuncheon-Hongcheon area, central Gyeonggi massif. Major mineral assemblage of quartzofeldspathic gneisses in the study area consists of biotite+ garnet+ sillimanite + plagioclase+ quartz${\pm}$kyanite${\pm}$K-feldspar${\pm}$muscovite. Kyanite occurs in four samples, and coexists with sillimanite in three of these samples. In most cases, kyanite is anhedral to subhedral, ranges up to Imm in the maximum dimension, and occurs as metastable relict grains. These observations indicate that the Yongduri gneiss complex has experienced a medium-pressure type metamorphism, followed by low-pressure type one belonging to the sillimanite+K-feldspar zone. Average temperature and pressure of the peak metamorphism are $683{\pm}62^{\circ}C$ and 4.9-5.5 kbar, respectively, when the existing chemical data are re-interpreted. In conjunction with the finding of kyanite in the Cheongpyeong-Gapyeong area (Lee and Cho, 19921, this study demonstrates that kyanite may occur regionally in central Gyeonggi gneiss complex. Moreover, the persistence of kyanite even after the high-T metamorphism of the sillimanitetK-feldspar zone suggests that the central Gyeonggi massif has experienced a tectonometamorphic evolution characterized by a rapid uplift.

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The necessary & method of scrotoplasty in female to male transgender (여성에서 남성으로의 성전환증에서 음낭성형술의 필요성과 방법)

  • Kim, Seok-Kwun;Moon, In-Sun;Kwon, Yong-Seok;Lee, Keun-Cheol
    • Archives of Plastic Surgery
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    • v.36 no.4
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    • pp.437-444
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    • 2009
  • Purpose: Transgender is a disorder of gender identity, who have appropriate chromosomal, hormonal and anatomical characteristics corresponding to their sexual phenotype but feel strongly with respect to their sexual identity, that they belong to the opposite sex. There is a persistence discomfort and sense of inappropriateness about one's assigned sex in a person who has reached puberty. Transgender is a psychiatric problem, but surgical method provides more satisfactory adjustment for patients. In gender reassignment surgery for female to male transgender, mastectomy, nipple reduction, hysterectomy, oophorectomy and phalloplasty are included. And as the final operation, recommended for scrotoplasty and artificial testes insertion. So we investigated the necessity and method of scrotoplasty in the final operation of female to male transgender. Method: The authors have long term follow-up of 75 cases female to male transgender during January, 1991 to February, 2008. Among them, 13 cases were evaluated in this study. During phalloplasty, the labium major skin preserved. And this labium majoral skin flap was made for the neoscrotum. At least six months later, artificial testes were inserted in neoscrotum with local anesthesia. Middle sized (3 cm diameter) artificial testes(silicon gel or carving soft silicone implant) were used because of the limitation of the neoscrotum. We evaluated the questionnaire and interview about the postoperative satisfaction in configuration of reconstructed scrotum, and the necessity of operation, the postoperative psychosocioeconomic improvement and limitation of body exposure activities such as swimming, public bathing. Results: Based on this study, satisfaction of reconstructed scrotum after scrotoplasty was improved(92%). The necessity of scrotoplasty was in 92.3% and the postoperative psychosocioeconomic well - being improvement was 77% in answers. Less limitation of activities requiring body exposure was 54% in answers. Most of the patients were satisfied with the results of surgical operation inspite of the operative procedure had some postoperative complications. Conclusion: This study was reported that the scrotoplasty in female to male transgender is not only a conversion of external genitalia but also an improvement of psychosocial state. Most patients sincerely hope to this operation, so we improve our surgical method for more good results.

Development of Overseas Construction Big Issues based on Analysis of Big Data (빅 데이터 분석을 통한 해외건설 빅 이슈 개발)

  • Park, Hwanpyo;Han, Jaegoo
    • Korean Journal of Construction Engineering and Management
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    • v.19 no.3
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    • pp.89-96
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    • 2018
  • This study derived big issues in overseas construction through big data analysis. To derive big issues in overseas construction, candidate groups of big issues were identified through big data analysis targeting 53,759 issues including 39,436 issues from major portal sites, 10,387 issues from daily newspapers, and 336 issues in construction magazines from Oct. 1, 2016 to Sep. 30, 2017. The main results are as follows: First, the main issues of overseas construction for the past one year showed that markets were concentrated in Middle East Asia and most of them were low-price order plant projects, which revealed the limitations. Although orders of overseas construction were slightly upward in the first half of 2017 compared to previous year, overseas construction orders are still unstable due to uncertainties in the international affairs and drops in oil prices. Second, the interest topics based on the 8th core keywords of overseas construction among the overseas construction issues for the past one year showed that region (29.9%), corporation environment (22.0%), profitability (17.0%), organizations (15.1%), projects (5.2%), market environment (3.6%), policy and system (3.6%), and education (3.5%) in the order of interest. Third, 10 core issues that have expandability and persistence of discourse were extracted out of 30 issue candidates with regard to eight keywords. Based on the extracted issues, detailed analysis on each of the core issues in overseas construction and correlation analysis between 10 core issues were conducted.

The Concentrations of Endocrine Disrupter (PCBs and DDE) in the Serumand Their Predictors of Exposure in Korean Women (일부 한국 성인 여성들의 혈중 내분비계 장애물질 농도 및 그 노출요인의 연구)

  • 민선영;정문호
    • Journal of Environmental Health Sciences
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    • v.27 no.2
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    • pp.127-137
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    • 2001
  • Polychlorinated biphenyls(PCBs) are halogenated aromatic compounds with the empirical formula $C_{12}$ $H_{10-n}$C $l_{n}$(n=1~10), and are a mixture of possible 209 different chlorinated congeners. PCBs were widely used as dielectric fluids for capacitors and transformers, plasticizers, lubricant inks and paint addirives. Once released into the environment, PCBs persist for years because they are so resistant to degradation. In addition to their persistence in the environment, PCBs in ecological food chains undergo biomagnification because of their high degree of lipophilicity. In 1970s, the worldwide production of PCBs was ceased and the import of PCBs was prohibited since 1983 in Korea. In spite of these actions, many PCBs seems to be still in use. The environmental load of PCBs will continue to be recycled through air, land, water, and the biosphere for decades to come. This study was conducted to measure the concentrations of PCBs in the serum samples of 112 women by GC/MSD and GC/ECD. The main results of this study were as follows. 1. PCBs were detected in all samples. The mean $\pm$SD levels of PCBs in the serum were 3.613$\pm$0.759 ppb, and median were 3.828 ppb. 2. The correlation coefficients of the concentrations of 13 PCB congeners were from minimum, 0.7913 to maximum, 0.9985, and all was significant(p=0.0001). The correlation coefficient between the concentrations of PCBs and p,p'-DDE was 0.9641(p=0.0001). 3. There was a positive association between age and PCBs' concentrations (simple linear regression ; $R^2$=0.86, $\beta$=0.08023, p<0.001). 4. There was a positive association between total lipids in the serum and PCBs' concentrations (simple linear regression ; $R^2$=0.7058, $\beta$=0.00486, p<0.001). 5. For possible predictors of PCBs and p,p' -DDE levels in the serum, age adjusted model (Y=$\beta$$_{0}$+$\beta$$_1$age+ $B_2$X) was applied. For BMI, major residential area, wether to eat caught fish by angling, where to eat caught fish by angling(by parents in the past), fish consumption, meat consumption, meat consumption, and dairy consumption, there was no association. For total conception frequency and lactation frequency and lactation period, there was negative association.ion.

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Weighted Analysis Method for Estimating the Orientation of Limestone Caves in Korea (가중치를 이용한 국내 석회동굴 발달 방향성 해석법 개발에 관한 연구)

  • Lee, Sang-Kyun;Park, Hyeong-Dong
    • The Journal of Engineering Geology
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    • v.24 no.1
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    • pp.39-52
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    • 2014
  • Limestone caves that consist of main passages and branches are formed by a variety of processes, and have the characteristic of developing with a preferred orientation controlled by discontinuities such as bedding, joints, and faults around the cave. However, it is difficult to analyze a representative orientation from various orientations. To interpret the overall development orientation of limestone caves, this study proposes new development orientation analysis methods, termed the Average Span Ratio Method (ASRM) and the Individual Development Ratio Method (IDRM), using the weighting of persistence. Nine limestone caves in Korea were randomly selected for testing the new methods. The methods show a stronger development orientation for limestone caves than that obtained by traditional methods, which consider only the distribution of development orientations. Based on an analysis of the relationship between the average span and the dip angle of bedding, it is confirmed that shallowly dipping bedding is a major contributor to the expansion of span in limestone caves. In addition, using scan-line survey data acquired in the field, we perform an RMR analysis of stability of the ground around limestone caves.

Molecular Detection of Virulence Factors in Carbapenem-Resistant Pseudomonas aeruginosa Isolated from a Tertiary Hospital in Daejeon (대전지역의 3차 병원에서 분리된 Carbapenem 내성 Pseudomonas aeruginosa의 병독성 인자 검출)

  • Cho, Hye Hyun
    • Korean Journal of Clinical Laboratory Science
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    • v.51 no.3
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    • pp.301-308
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    • 2019
  • The emergence and spread of multidrug resistant (MDR) Pseudomonas aeruginosa is a critical problem worldwide. The pathogenesis of P. aeruginosa is due partly to the production of several cell-associated and extracellular virulence factors. This study examined the distribution of virulence factors and antimicrobial resistance patterns of carbapenem-resistant P. aeruginosa (CRPA) isolated from a tertiary hospital in Daejeon, Korea. Antimicrobial susceptibility testing was performed using the disk diffusion method, and PCR and DNA sequencing were performed to determine for the presence of virulence genes. In addition, the sequence type (ST) of MDR P. aeruginosa was investigated by multilocus sequence typing (MLST). Among 32 CRPA isolates, 14 (43.8%) were MDR and the major ST was ST235 (10 isolates, 71.4%). All isolates were positive for the presence of virulence genes and the most prevalent virulence genes were toxA, plcN, and phzM (100%). All isolates carried at least eight or more different virulence genes and nine (28.1%) isolates had 15 virulence genes. The presence of the exoU gene was detected in 71.4% of the MDR P. aeruginosa isolates. These results indicate that the presence of the exoU gene can be a predictive marker for the persistence of MDR P. aeruginosa isolates.

A Study of Film Crews' Needs to Participate in Overseas Production : On the Basis of Binary Logistics Analysis and Marginal Effect Method (영화제작인력들의 해외활동 참여욕구에 관한 연구 : 이분형 로지스틱 분석 및 한계효과분석을 중심으로)

  • Lee, Hee-Jin
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.4
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    • pp.27-37
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    • 2019
  • This study empirically identified and sought implications for the degree of desire by film crews to participate in overseas production and the factors affecting them, based on the recognition that the active participation of skilled film crews in overseas production and joint ventures can be an alternative to the poor treatment of domestic film industry sites and the reduction of job losses due to problems with career barriers. In order for respondents to verify research questions, binomial logistic and marginal effects analysis using SPSS and STATA, the data obtained by surveying 402 film crews operating in various areas. The notable findings are as follow: First, about 65%(263 people) of the respondents expressed their desire to participate in overseas activity, indicating that a considerable number of film crews are looking forward to opportunities with overseas productions. Second, major factors that have a positive effect on film crews' need to participate in overseas activities have been found to be professionality, artistic identity, Korean Wave persistence, and career barriers. In other words, the higher the professionality, the more clearly the identity of the artist, the more positive expectations of the possibility of the Korean Wave continuing, and the more serious the problem of career disability, the clearer was the desire to participate in overseas production and joint-work activities.