• Title/Summary/Keyword: Major crop

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Genetic Diversity of Barley Cultivars as Revealed by SSR Masker

  • Kim, Hong-Sik;Park, Kwang-Geun;Baek, Seong-Bum;Suh, Sae-Jung;Nam, Jung-Hyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.5
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    • pp.379-383
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    • 2002
  • Allelic diversity of 44 microsatellite marker loci originated from the coding regions of specific genes or the non-coding regions of barley genome was analyzed for 19 barley genotypes. Multi-allelic variation was observed at the most of marker loci except for HVM13, HVM15, HVM22, and HVM64. The number of different alleles ranged from 2 to 12 with a mean of 4.0 alleles per micro-satellite. Twenty-one alleles derived from 10 marker loci are specific for certain genotypes. The level of polymorphism (Polymorphic Information Content, PIC) based on the band pattern frequencies among genotypes was relatively high at the several loci such as HVM3, HVM5, HVM14, HVM36, HVM62 and HVM67. In the cluster analysis using genetic similarity matrix calculated from microsatellite-derived DNA profiles, two major groups were classified and the spike-row type was a major factor for clustering. Correlation between genetic similarity matrices based on microsatellite markers and pedigree data was highly significant ($r=0.57^{**}$), but these two parameters were moderately associated each other. On the other hand, RAPD-based genetic similarity matrix was more highly associated with microsatellite-based genetic similarity ($r=0.63^{**}$) than coefficient of parentage.

Analysis of Korean japonica rice cultivars using molecular markers associated with blast resistance genes

  • Suh, Jung-Pil;Roh, Jae-Hwan;Cho, Young-Chan;Han, Seong-Sook;Jeon, Yong-Hee;Kang, Kyung-Ho;Kim, Yeon-Gyu
    • Korean Journal of Breeding Science
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    • v.40 no.3
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    • pp.215-222
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    • 2008
  • Fifty-two Korean japonica rice cultivars were analyzed for leaf blast resistance and genotyped with 4 STS and 26 SSR markers flanking the specific chromosome sites linked with blast resistance genes. In our analysis of resistance genes in 52 japonica cultivars using STS markers tightly linked to Pib, Pita, Pi5(t) and Pi9(t), the blast nursery reaction of the cultivars possessing the each four major genes were not identical to that of the differential lines. Eight of the 26 SSR markers were associated with resistant phenotypes against the isolates of blast nursery as well as the specific Korean blast isolates, 90-008 (KI-1113), 03-177 (KJ-105). These markers were linked to Pit, Pish, Pib, Pi5(t), Piz, Pia, Pik, Pi18, Pita and Pi25(t) resistance gene loci. Three of the eight SSR markers, MRG5836, RM224 and RM7102 only showed significantly associated with the phenotypes of blast nursery test for two consecutive years. These three SSR markers also could distinguish between resistant and susceptible japonica cultivars. These results demonstrate the usefulness of marker-assisted selection and genotypic monitoring for blast resistance of rice in blast breeding programs.

Classification of Summer Paddy and Winter Cropping Fields Using Sentinel-2 Images (Sentinel-2 위성영상을 이용한 하계 논벼와 동계작물 재배 필지 분류 및 정확도 평가)

  • Hong, Joo-Pyo;Jang, Seong-Ju;Park, Jin-Seok;Shin, Hyung-Jin;Song, In-Hong
    • Journal of The Korean Society of Agricultural Engineers
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    • v.64 no.1
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    • pp.51-63
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    • 2022
  • Up-to-date statistics of crop cultivation status is essential for farm land management planning and the advancement in remote sensing technology allows for rapid update of farming information. The objective of this study was to develop a classification model of rice paddy or winter crop fields based on NDWI, NDVI, and HSV indices using Sentinel-2 satellite images. The 18 locations in central Korea were selected as target areas and photographed once for each during summer and winter with a eBee drone to identify ground truth crop cultivation. The NDWI was used to classify summer paddy fields, while the NDVI and HSV were used and compared in identification of winter crop cultivation areas. The summer paddy field classification with the criteria of -0.195

Identification of a Novel Bakanae Disease Resistance QTL in Zenith Cultivar Rice (Oryza sativa L.)

  • Sais-Beul Lee;Jun-Hyun Cho;Nkulu Rolly Kabange;Sumin Jo;Ji-Yoon Lee;Yeongho Kwon;Ju-Won Kang;Dongjin Shin;Jong-Hee Lee;You-Cheon Song;Jong-Min Ko;Dong-Soo Park
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.12a
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    • pp.64-64
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    • 2020
  • Bakanae disease, caused by several Fusarium species, imposes serious limitations to the productivity of rice across the globe. The incidence of this disease has been shown to increase, particularly in major rice-growing countries. Thus, the use of high resistant rice cultivars offers a comparative advantage, such as being cost effective, and could be preferred to the use of fungicides. In this research, we used a tropical japonica rice variety, Zenith, a bakanae disease resistant line selected as donor parent. A RIL population (F8:9) composed of 180 lines generated from a cross between Ilpum and Zenith was used. In primary mapping, a QTL was detected on the short arm of chromosome 1, covering about 3.5 Mb region flanked by RM1331 and RM3530 markers. The resistance QTL, qBK1Z, explained about 30.93% of the total phenotype variation (PVE, logarith of the odds (LOD) of 13.43). Location of qBK1Z was further narrowed down to 730 kb through fine mapping using additional RM markers, including those previously reported and developed by Sid markers. Furthermore, there is a growing need to improving resistance to bakanae disease and promoting breeding efficiency using MAS from qBK1Z region. The new QTL, qBK1Z, developed by the current study is expected to be used as foundation to promoting breeding efficiency with an enhanced resistance against bakanae disease. Moreover, this study provides useful information for developing resistant rice lines carrying single or multiple major QTLs using gene pyramiding approach and marker-assisted breeding.

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