• Korean Journal of Medicinal Crop Science
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• v.17 no.2
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• pp.145-150
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• 2009

This study investigated the protective roles and mechanism of magnolol, from the stem bark of Magnolia officinalis against potential neurotoxin 3-hydroxykynurenine (3-HK)-induced neuronal cell death. For the evaluation of protective role of magnolol, we examined cell viability, apoptotic nuclei, change of mitochondrial membrane potential and caspase activity in human neuroblastoma SH-SY5Y cells. It was found that 3-HK induces neuronal cell death in the human neuroblastoma SH-SY5Y cell line. The reduced cell viability produced characteristic features such as cell shrinkages, plasma membrane blebbing, chromatin condensation, and nuclear fragmentation. The cells treated with 3-HK showed an increase in the concentration of reactive oxygen species (ROS) as well as in caspase activity. In addition, both are involved in the 3-HK-induced apoptosis. Magnolol attenuated the cell viability reduction by 3-HK in both a dose- and time-dependent manner. Optical microscopy showed that magnolol inhibited the cell morphological features in the 3-HK-treated cells. Furthermore, the increase in the ROS concentration and the caspase activities by 3-HK were also attenuated by magnolol. These results showed that magnolol has a protective effect on the 3-HK induced cell death by inhibiting ROS production and caspase activity.

• Journal of Acupuncture Research
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• v.25 no.2
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• pp.75-89
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• 2008

목적 : 후박(厚朴)(Magnolia obovata)에서 추출한 낮은 농도의 obovatol 약침액의 RAW264.7 세포에서 LPS로 유발된 염증, $TNF-{\alpha}$로 유발된 human colon carcinoma SW620 및 HCT116 세포의 세포증식에 대한 영향과 그 기전을 살펴보고자 하였다. 방법 : RAW264.7 세포에서 LPS로 염증을 유발하고 낮은 농도의 obovatol 약침액을 처리한 후 cell viability, NO 생성량, iNOS와 COX-2의 발현, $NF-{\kappa}B$활성, 전사능력을 관찰하기 위해 WST-1 assay, NO determination assay, western blot analysis, EMSA, luciferase activity assay를 시행하였고, HCT116, SW620 세포에 $TNF-{\alpha}$로 증식을 유도하고 낮은 농도의 obovatol 약침액을 처리한 후 cell growth, apoptosis 및 apoptosis와 연관된 $NF-{\kappa}B$의 활성 변화를 관찰하기 위해 WST-1, Cell morphogy test, DAPI staining and TUNEL assay, EMSA, luciferase activity assay를 시행하였다. 결과 : 1. RAW264.7 세포에서 낮은 농도의 obovatol 약침액 처리는 $NF-{\kappa}B$의 활성 및 전사능력을 낮추고 iNOS와 COX-2의 발현과 NO 생성을 감소시켜 LPS로 유발된 염증을 억제하였다. 2. HCT116, SW620 세포에서 낮은 농도의 obovatol 약침액 처리는 $NF-{\kappa}B$의 활성을 낮추어 세포자멸사를 촉진함으로써 $TNF-{\alpha}$로 유발된 암세포의 성장을 억제하였다. 결론 : 이상의 결과는 낮은 농도의 obovatol 약침액이 항염 및 인간 전립선암세포주인 SW620, HCT116에 대한 증식억제 효과가 있음을 입증한 것이며, 향후 이를 바탕으로 한 생체 연구에서의 긍정적인 결과는 obovatol 약침액이 만성염증성 질환 및 대장암의 예방과 치료에 대한 효과적인 치료제 개발에 초석이 될 것으로 기대된다.

• Korean Journal of Environment and Ecology
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• v.6 no.2
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• pp.113-126
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• 1993

To investigate the structure of the forest community of Huibang valley in Sobaeksan National Park, twenty five plots were set up and surveyed. According to the analysis of classification by DCA community divided 4 groups. The divided groups are Pinus densiflora community, Q. mongolica -P. densiflora community. Q. mongolica-Stewartia koreana-Carpinus laxiflora community, and Q. mongolica- Acer pseudosieboldianum community. In the analysis by ordination, CCA which points out the relationship among the environmental variables, species and plots indicated primary step of secondary succession. The successional trends of both techniques. seem to be from P. densiflora through Q. mongolica, Q. serrate to C. laxiflora(or S. koreana) in canopy layer below an altitude of 800 meters, and from Rhododendron mucronulaltum. Rh. schlippenbachli. Rhus trichocarpa through Styrax obassia. Lindera obtusiloba to Magnolia sieboldii, Corylus sieboldiana in subtree and shrub layer. The positive correlation between the stand ordination scores .and soil moisture and soil humus were calculated.

• The Korean Journal of Ecology
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• v.19 no.4
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• pp.253-261
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• 1996

This study was conducted to clarify the relationship between bird community and forest structure from early May to mid June, 1988 in two sites which had different forest structures due to silvicultural practice, located in the Tomakomai Experimental Forest of Hokkaido University, Hokkaido, Japan. Quantitative ahbitat analysis was applied to elucidate difference between the two study sites, and territory mapping method and guild analysis were used for assessment of the bird community. Dominant tree species were Quercus mongolica, Magnolia obovata, Prunus sargentii, Acer mono and Tilia japonica. Unithinned site had more foliage coverage in upper and middle layers, but in lower layer had less coverage than thinned site. Thirty four species of birds were recorded and of them only 21 species occupied territories within the study area. Dominant species were Ficedula narcissina, Phylloscopus occipitalis, Parus major, Passer rutilans, Sitta europaea and Parus palustris in the study area. The unmber of breeding species of the two sites were equal, but the species composition of breeding bird communities was different between the sites. The number of species and pairs on hole-nesting guild were greater in the unthinned site, but those on ground-nesting guild were greater in the thinned site. Canopy-nesting guild showed no significant difference between the two sites. Silvicultural practice such as proper thinning appeared to be not inadequate to all of the forest birds, probably good to bush and ground nesting guild, and to tree & bush and ground foraging guild. The silvicultural practice also did not considerably affect the hole and canopy-nesting guild.

• Journal of the Korean Institute of Landscape Architecture
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• v.31 no.2
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• pp.94-101
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• 2003

The purpose of this paper is to analyze the correlation between Death Rate of Trees (DRT) and the Physiological Characteristics of Trees(PCT) in POSCO Gwangyang works, which is a reclaimed area. To analyze the DRT, 15 species of deciduous trees were selected, for example Ulmus davidiana var., Zelkova serrata, Melia azedarach var. etc. Though there were numerous factors to affect the growing of trees, 5 PCT were considered to be main factors, soil salt tolerance, wind salt tolerance, water needs, transplanting difficulty, and nutrient needs. According to two kinds of soil-base: mound and pot area, we tested the relationship between 5 PCT and DRT by use of t-test and multiple regression analysis. The results are as follows. 1. The DRT of Acer palmatum, Cornus kousa, Magnolia kobus, Liriodendron tulipifera, and Albizzia julibrissin were high by more than 20%. On the other hand, Chionanthus retusa, Ulmus davidiana var. japonica Celtis sinensis, and Lagerstroemia indica were low by less than 10% in the DRT and are considered to be species suitable for planting in reclaimed areas. 2. The DRT of trees in pot areas was meaningfully higher than in mound areas; for this reason the mound technique is desirable as a soil-base for planting in reclaimed areas. 3. In the pot area, the independent variables, in the order of soil salt tolerance, wind salt tolerance, transplanting difficulty, had an effect on the DRT more significantly than in mount area. On the other hand, wind salt tolerance and soil salt tolerance affected the DRT in mount areas. This means that soil salt tolerance, wind salt tolerance, and transplanting difficulty have to be considered as significant factors to the DRT. Although the researchers tried to interpret how the PCT affected the DRT in order to analyze the relationship between the two in reclaimed areas, it was neglected at an experimental level. Therefore, future research should work on this aspect in detail.

• Korean journal of applied entomology
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• v.44 no.1 s.138
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• pp.51-59
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• 2005

A total of 45 scale insects belonging to 29 genera of five families were recognized on 15 kind of flowering plants on the basis of specimens collected in 2003 and specimens deposited in the Insect Collection of NIAST in Korea. Three species were recognized on Korean forsythia, four on chrysanthemum, 15 on common camellia, seven on kobus magnolia, three on rose of sharon, one on Indian lilac, six on rose, seven on rhododendrons, two on bamboo palm, three on benjamin tree, 12 on evergreen euonymus, five on Japanese yew, two on corn plant, six on orchids and two on cacti. Pulvinaria floccifera (Westwood) and Pseudaonidia paeoniae (Cockerell) on common camellia and Pulvinaria citricolar Kuwana on rose of sharon are reported for the first time from the Korean Peninsula.

• Applied Biological Chemistry
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• v.32 no.4
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• pp.367-373
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• 1989

The effect of gelatin concentration, pH of fruit juice and other factors for the enzymatic clarification of fruit juice by the use of polygalacturonase was studied. The proper addition of gelatin was much effected on clarification by polygalacturonase and optimum concentrations of gelatin for the enzymatic clarification of Zugaru juice, Magnolia Gold juice, Golden Delicious juice, Jonagold juice, Jonathan juice, Campbell Early juice were 0.04, 0.01% 0.02%, 0.06% 0.01%, and 0.04%, respectively. Optimum pH for the clarification by the use of polygalacturonase and gelatin was $3.2{\sim}3.5$ contrary to optimum pH 4.8 of polygalacturonase for the hydrolysis of pectic acid. At the reaction temperature of $45^{\circ}C$ and polygalacturonase concentration of 0.01mg/ml, fruit juices were completely clarified by optimum addition of gelatin for 60min.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.6
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• pp.1528-1532
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• 2003

To investigate the antioxidant capacity of traditional Korean medicines, water extracts from 57 species were tested on their antioxidant activity using radical scavenging effects against ABTSㆍ+. Of which, Rhus javanica, Caesalpinia sappan, Rosa rugosa, Spatholobus suberectus, and Magnolia obovata showed strong antioxidant capacities at 10㎍ concentration. Therefore, antioxidant capacities of 5 traditional medicine extracts in the different concentration (1㎍, 5㎍, and 10㎍) were determined. The 5 traditional medicine extracts was detected in antioxidant capacity dose dependently. R. javanica was showed the highest antioxidant capacity, the antioxidant activity at 1 ㎍ of herbal extract being 0.85 mM TE. At the same time, the antiperoxidation effects of these 5 medicines were determined. Lipid peroxidation in brain homogenates induced by NADPH and ADP-Fe/sup 2+/ was strong inhibited by S, suberectus, R. rugosa, and M. obovata. Extract of C. sappan was showed the highest inhibition against lipid peroxidation compared with 4 herbal extracts in vitro. These traditional medicines are a potent antioxidant capacity and antiperoxidation activity, further investigation into the in vivo antioxidant therapeutic potential for treatment of human disorders such as Alzheimer's disease.

• Journal of the Korean Society of Fashion and Beauty
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• v.5 no.1 s.12
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• pp.51-55
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• 2007

These days, hair design is recognized as a kind of molding arts which peform expression skill to create personal self as well as practical skill. Recently, the efforts to express hair design sublimating it to art works have continued. For these reasons, this study had purposes as follows; First, it developed creative and original design producing works to shape the flower image that was an important material of hair design. Second, it presented the possibility to express the field of hair design with art. In the flow of age, nature has provided design with unlimited creative motive. Flowers, among various materials, show the nature's change, combination and order and impose diverse symbolic meanings. Therefore, flowers are good materials to express the works. Through the process of decolorizing and dyeing with hair, five works of nature's fragrance, hope, reed flowers, windflower, magnolia blossom had been produced. The results of the process were as follows; First, the transformation of shape through the simple process taking the image of shaping flower as subject matter could be a motive of new hair design art. Second, if the various images of flowers were expressed as hair art with three-dimensional shape, it could be works with value of beauty. Through the process of this study, it was proved that nature could be endless subject matter for art. Therefore, with continuous studies, it can be motive of developing designs in producing work activities of many hair designers. In addition, academic development will be achieved through wide and diverse studies.

• Korean Journal of Pharmacognosy
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• v.30 no.4
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• pp.384-396
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• 1999

Acyl-CoA: Cholesterol Acyltransferase (ACAT) is a key enzyme responsible for cholesteryl ester formation in atherogenesis and in cholesterol absorption from the intestines. In addition under pathological conditions, formation and accumulation of cholesteryl ester as lipid droplets by ACAT within macrophages constitute a characteristic feature of early lesions of atherosclerotic plaques. ACAT inhibitors are expected to be effective for treatment of atherosclerosis and hypercholesterolemia. ACAT inhibitors of natural origin have been rarely reported. In our screening program for ACAT inhibitors, 303 plants were extracted with methanol or ethanol, and screened for the inhibitory activity against ACAT from the rat liver microsome. Extracts of 13 plants including Quercus aliena, Diospyros kaki, Platycarya strobilacea and Hibiscus syriacus inhibited more than 90% of ACAT activity and 43 samples in alcohol extracts such as Magnolia obovata and Panax ginseng also inhibited more than 70% of ACAT activity at a concentration of $100\;{\mu}g/ml$.