• 제목/요약/키워드: Magnetic Resonance Spectroscopy

검색결과 624건 처리시간 0.022초

Advanced techniques of solution nuclear magnetic resonance spectroscopy for structural investigation of protein-protein interaction

  • Sugiki, Toshihiko;Lee, Young-Ho
    • 한국자기공명학회논문지
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    • 제22권4호
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    • pp.76-81
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    • 2018

  • Investigation of the protein-protein interaction mode at atomic resolution is essential for understanding on the underlying functional mechanisms of proteins as well as for discovering druggable compounds blocking deleteriou interprotein interactions. Solution NMR spectroscopy provides accurate and precise information on intermolecular interactions even for weak and transient interactions, and it is also markedly useful for examining the change in the conformation and dynamics of target proteins upon binding events. In this mini-review, we comprehensively describe three unique and powerful methods of solution NMR spectroscopy, paramagnetic relaxation enhancement (PRE), pseudo-contact shift (PCS), and residual dipolar coupling (RDC), for the study on protein-protein interactions.

  • https://doi.org/10.6564/JKMRS.2018.22.4.076 인용 PDF KSCI HTML

In-cell nuclear magnetic resonance spectroscopy for studying intermolecular interactions

  • Sugiki, Toshihiko;Lin, Yuxi;Lee, Young-Ho
    • 한국자기공명학회논문지
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    • 제23권1호
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    • pp.33-39
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    • 2019

  • Studies on the interactions of proteins with partner molecules at the atomic resolution are essential for understanding the biological function of proteins in cells and for developing drug molecules. Solution NMR spectroscopy has shown remarkably useful capability for investigating properties on the weak to strong intermolecular interactions in both diluted and crowded solution such as cell lysates. Of note, the state-of-the-art in-cell NMR method has made it possible to obtain atomistic information on natures of intermolecular interactions between target proteins with partner molecules in living cells. In this mini-review, we comprehensively describe the several technological advances and developments in the in-cell NMR spectroscopy.

  • https://doi.org/10.6564/JKMRS.2019.23.1.033 인용 PDF KSCI HTML

Magnetic Resonance Imaging Meets Fiber Optics: a Brief Investigation of Multimodal Studies on Fiber Optics-Based Diagnostic / Therapeutic Techniques and Magnetic Resonance Imaging

  • Choi, Jong-ryul;Oh, Sung Suk
    • Investigative Magnetic Resonance Imaging
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    • 제25권4호
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    • pp.218-228
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    • 2021

  • Due to their high degree of freedom to transfer and acquire light, fiber optics can be used in the presence of strong magnetic fields. Hence, optical sensing and imaging based on fiber optics can be integrated with magnetic resonance imaging (MRI) diagnostic systems to acquire valuable information on biological tissues and organs based on a magnetic field. In this article, we explored the combination of MRI and optical sensing/imaging techniques by classifying them into the following topics: 1) functional near-infrared spectroscopy with functional MRI for brain studies and brain disease diagnoses, 2) integration of fiber-optic molecular imaging and optogenetic stimulation with MRI, and 3) optical therapeutic applications with an MRI guidance system. Through these investigations, we believe that a combination of MRI and optical sensing/imaging techniques can be employed as both research methods for multidisciplinary studies and clinical diagnostic/therapeutic devices.

  • https://doi.org/10.13104/imri.2021.25.4.218 인용 PDF KSCI