• Journal of Animal Environmental Science
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• v.5 no.1
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• pp.37-44
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• 1999

This study was carried out to improve utilization of substitute fly-ash in bedding material of animal waste treatments. The amount used of fly-ash used in a pigpen or beef stall was 50% lower than that of existing bedding material of animal waste treatments. From the results, substitution effect of fly-ash put over the floor of the stable became much better. Effects of processed fly ash as a spread straw decreased ammonia(NH3) and Hydrogensulfide (H2S) gas at beef stall, but there was no benefit of replacement terms. Effect of processed fly ash as a spread straw increased 4∼5 times replacement terms more than control NH3 and H2S gas was decreased. A lot of maggots and porasites were grown at sawdust pig farm, but fly ash inhibited to grow maggots and paraeters. In conclusion, as substituting fly-ash for 5% sawdust(DM basis) in making animal waste into a compost with fly ash, we can reduce the sawdust purchasing costs and produce the high quality of a compost, especially a pollutant as NH3 and H2S gas, etc. from the process of biodegradation, and as substituting fly-ash(1,540 won per ton ; can be extended the replacement period of spreading straw approximatively 4∼5 times) for sawdusts(111,000 won per ton) will increase a real income in livestock house.

• Korean journal of applied entomology
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• v.35 no.2
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• pp.132-136
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• 1996

These studies were carried out to investigate the occurrence of rice insect pests related to different transplanting times and N-P-K-fertilizer levels of paddy field in Southern region of Korea. The population densities of brown planthopper (BPH: Nilaparvata lugens Stal), whitebacked planthopper (WBPH: Sogatella furcifera Horvath), small brown planthopper (SBPH: Laodelphax striatellus Fallen), green rice leafhopper (GRLH: Nephotettix cincticeps Uhler), rice stem maggot (RSM: Chlorops oryzae Matsumura), striped rice borer (SRB: Chilo suppressalis Walker), and rice leaffolder (RLF: Cnaphalocrocis medinalis Guenee) were affected more by transplanting time than the fertilizer levels. The later transplanting time induced the higher population densities of BPH, WBPH, SBPH, GRLH, RSM, whereas SRB and RLF were affected by earlier transplanting time in paddy field. The major pests except GRLH and SRB were increasingly induced by higher N-fertilizer level in the late transplanting.

• Journal of Veterinary Clinics
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• v.32 no.5
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• pp.473-475
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• 2015

A 12-year-old intact male, Alaskan Malamute dog, which lives in the countryside, was presented with inflammation and pain around perineal areas. Thorough examination revealed maggots and punched-out round holes lesion around the perineal region. Complete blood counts (CBC) and serum biochemical examinations showed no remarkable findings except mild anemia and mild thrombocytosis. The diagnosis was easily done, based on clinical signs and maggots identification. Cleaning with chlorhexidine, povidone-iodine lavage and hair clipping away from the lesions were performed soon after presentation. SNAP 4Dx Test (IDEXX Laboratories, Westbrook, ME, USA) was performed to rule out other vector-borne diseases since the ticks were found on the clipped area and vector-borne pathogens. The test result was negative. The dog in this case was treated with ivermectin (300 mcg/kg SC) one time. Also, treatments with amoxicillin clavulanate (20 mg/kg PO, BID) was established to prevent secondary bacterial infections. Then, myiasis resolved with 2 weeks and the affected area was healed.

• Korean journal of applied entomology
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• v.23 no.4 s.61
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• pp.233-236
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• 1984

Studies were conducted to investigate the effect of Carbofuran 3G on the density variation of rice pests and their natural enemies in case the insecticide was treated two times as compared with usual trials in paddy field. Even the two times application of Carbofuran 3G in the nursery boxes of machine transplanting and paddy water trials obtain same results to pests, small brown planthopper (Laodelphax striatllus), green rice leafhopper (Nephotettix cincticeps), brown planthorper (Nilaparvata lugens), white back planthopper (Sogotella furcifera), rice stem bore (Chilo suppressalis), rice stem maggot (Chlorops oryzae), rice thrips (cloethrips oryzae) as compared with usual trials. The density of natural enemies, spiders and egg parasite, Anagrus sp. of planthoppers in the two times application part of Carbofuran 3G increased 2.4 fold as compared with usual trials.

• BMB Reports
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• v.39 no.6
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• pp.749-758
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• 2006

The cytosolic members of the HSP70 family of proteins play key roles in the molecular chaperone machinery of the cell. In the study we cloned and sequenced the full-length cDNA of Delia antiqua HSP70 gene, which is 2461 bp long and encodes 643 a.a. with a calculated molecular mass of 70,787 Da. We investigated gene copies of cytosolic HSP70 members of 4 insect species with complete genome available, and found that they are quite variable with species. In order to characterize this protein we carried out an alignment and a phylogenetic analysis with 41 complete protein sequences from insects. The analysis divided the cytosolic members of the family into two classes, HSP70 and HSC70, distinguishable on the basis of 15 residues. HSP70 class members were slightly shorter in length and smaller in molecular mass relative to the HSC70 class members, and the conservative and functional regions in these sequences were documented. Mainly, we investigated the expression of Delia antiqua HSP70 gene, in response to diapauses and thermal stresses. Both summer and winter diapauses elevated HSP70 transcript levels. Cold-stress led to increased HSP70 expression levels in summer- and winter-diapausing pupae, but heat-stress elevated the levels only in the winter-diapausing pupae. In all cases, the expression levels, after being elevated, gradually decreased with time. HSP70 expression was low in non-diapausing pupae but was up-regulated following cold- and heat-stresses. Heat-stress gradually increased the mRNA level with time whereas cold-stress gradually decreased levels after an initial increase.

• Journal of the Korean Applied Science and Technology
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• v.31 no.4
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• pp.572-583
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• 2014

This study was carried out to determine the action mechanism of sulfur-grain larvae extract (SGE) on anti-obesity and the reduction of blood lipid level in high-fat diet induced obese model animals. Animals were classified into a normal diet group (NC, normal control), HFD (high-fat diet without SGE), HFD 15 (high-fat diet + oral administration of 15 mg of SGE extract per 100 g body weight) and HFD 30 (high-fat diet + oral administration of 30 mg of SGE extract per 100 g body weight). The body weight gain declined in HFD 15 and HFD 30 groups compared with the HFD group, even though the diet intake increased significantly. The weight of liver and adipose tissue increased significantly in HFD group compared with in the HFD 15 and HFD 30 groups. Triglyceride, total cholesterol, LDL-C and AI decreased in HFD 15 and HFD 30 groups compared with in the HFD group, but the contents of HDL-C increased significantly. Expression of SREPB-$1{\alpha}$, SREPB-2 mRNA in the liver was lower in the high-fat diet group compared with the HFD group, but the expression of LPL mRNA in adipose tissue and $PPAR{\alpha}$ increased significantly. Fat accumulation in the liver tissues and liver damage were greatly reduced in HFD 15 and HFD 30 groups compared with in the HFD group. The size of adipocytes became smaller in the HFD 15 and HFD 30 groups compared with HFD group. In conclusion, this research discovered for the first time that grain maggot has anti-obesity effects, by reducing the abdominal fat of obese model animals and lowering blood lipid level through the down-regulation of PPAR-$1{\alpha}$ and SREPB-2 mRNA and the up-regulation of PPAR-${\alpha}$ mRNA.

• Korean Journal of Plant Resources
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• v.12 no.2
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• pp.102-106
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• 1999

This experiment was carried out to find the method for mass production by artifical cultivition and to analyze the components of Paecilomyces japonica according to several media. Time of inoculation of the Paecilomyces japonica using silkworm was on first day of five molting and infection rate was 72.0%. Optium medium for mass production of the Paecilomyces japonica was known effective for increasing dry weight and fruitbody at brown rice 80g plus pupa powder 20g. Dry weight of Paecilomyces japonica using fungus of silkworm was 1.2g including pupa and length of fruitbody was appeared 3.0cm to 3.5cm. Content of $\beta$ - glucan was very high as 40.5% at inoculation on the first day of the five molting while 16.4% at brown rice, 20.7% at pupa, 23.1% at brown rice plus pupa powder, and 28.7% at pine sawdust plus wheat bran. Mycelium was poor and pinkly conidiospore was formed on media of centipede, maggot and powder of silkworm.

• Korean journal of applied entomology
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• v.24 no.1 s.62
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• pp.29-33
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• 1985

Every plant in $990m^2$ onion field was inspected for damages by the onion maggot. Maps were constructed every ten days to show which plants were infested and which were not from April 11 to May 21, 1984. The maps were sectioned into squares one of which contains 80 onion plants and the counts of damaged onions in each square were fitted to poisson and negative binomial distribution and tested by chi-square. We argue that the satisfactory fitness of the expected negative binomial $[P(x^2)>0.05]$ provided a useful description of the spatial distribution patterns of the damaged onions. Edge effect was tested by the differences of damage ratio and variance/mean ratio (${\sigma}^2/m$) between edge and center part. The result showed that the damage ratioes and variances of all the periods, ${\sigma}^2/m$ values after May 1 were greater in edge part than in center part. Again, the maps were sectioned into four blocks and the squares (sample units) were sectioned into quadrants. By application of the variance component technique, it was suggested that $2{\sim}8$ sample units for 5% sampling error and $1{\sim}2$ sample units for 10% error should be sampled randomly to estimate the damage ratio when $2{\sim}3$ quadrants were inspected.

• Korean journal of applied entomology
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• v.22 no.4 s.57
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• pp.271-276
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• 1983

In field studies, the insecticides carbofuran(Furadan 3G) and diazion(3G) at the rate of 1,8 kg a.i./ha as a single side-furrowing treatment from March 13 to April 10(7 to 10 days intervals) were evaluated in terms of the control effects to the onion maggots(Hylemyia antique Meigen) and some phytotonic effects to the garlic plants. The treatments with carbofuran were significantly more effective in onion maggot control than the treatment with diazinon and untreated, and the earlier treatments the better control effects were obtained. Phytotonic effects only with carbofuran on garlic plants were of served as increases ranged $16.5\~23.2\%$ in plant heights, $22.0\~32.0\%$ in stem diameters and $15.5\~22.8\%$ in bulb diameters compared to the untreated. The greatest yield of garlics was resulted in the plots of Match-13 treatments with carbofuran, ca. 10,700kg/ha in the carbofuran treatment, ca. 5637kg/ha in the diazinon treatment and ca. 4728kg/ha in the untreated plots. The magnitude of yield increase of garlics in the plots treated with carbofuran was attributable to positively stimulate the growth response of garlic plants as well as the control effects to onion maggots.

• Parasites, Hosts and Diseases
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• v.54 no.5
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• pp.667-671
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• 2016

Myiasis is a relatively common infection of animals kept as pets, although only 1 case of canine myiasis has been described so far in the Republic of Korea. In the present study, we report an additional case of canine wound myiasis with identification of its causative agent, Lucilia sericata. An 8-year-old male Siberian husky dog was referred with anorexia, vomiting, and diarrhea to the Chungbuk National University Veterinary Medical Center, Cheongju-si (city), Chungcheongbuk-do (province), Korea in July 2013. Physical examination indicated the patient had a deep wound filled with a maggot swarm as a left gluteal lesion. A total of 216 maggots were removed by forceps, and the wounded area was sponged with gauzes and disinfected with 70% alcohol and a povidone-iodine solution. After daily care and suturing the wound, the patient was discharged at day 19 after admission. Recovered worms possessed morphological characteristics similar to those of L. sericata, namely, a sub-cylindrical body with 6-8 lobed anterior spiracles, round shaped with a button surrounded by a peritremal ring with no gaps, and similar distances between dorsal, median, and outer papillae of the 12th segment. Additionally, cox1 partial sequences (528 bp) obtained in the present study showed 100% identity with those of L. sericata (GenBank no. KT272854.1). L. sericata is indicated as a pathogen of myiasis infection not only in humans, but also in animals kept as pets in Korea.