• Applied Biological Chemistry
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• 제19권3호
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• pp.121-129
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• 1976

우유중에 상당량 존재하는 myo-Inositol의 생성을 알아보기 위하여 3% SE-52를 충진시킨 gas-liquid chromatography에 의해 myo-Inositol의 정량을 행하고 이 myo-Inositol의 유래를 feeding 실험과 $(^{14}C)-glucose$와 (3H)-myo-Inositol을 쥐에 주사한 실험으로 알아 븐 결과 다음과 같은 결과를 얻었다. 1. 쥐우유를 G.L.C. 방법으로 분석하여 우유 100gm.당 61-91mg.의 myo-Inositol (free)을 얻었다. 2. Feeding 실험 결과 우유 myo-Inositol의 85%는 diet에서 흡수되었고 나머지는 mammary gland에서 합성된 것으로 나타났다. 3. $(^{14}C)-glucose$에서 $(^{14}C)-myo-Inositol$ 생성은 lactose의 생성과 비슷하게 한시간이내에 최고에 달하였다. 4. $(^3H)-myo-Inositol$을 lactating rat에 주사하여 실험한 결과 우유 myo-Inositol의 대부분은 혈액 myo-Inositol에서 유래되었다. $(^{14}C)-glucose$를 주사한 후 우유중에 나타난 myo-Inositol의 비방사능(比放射能)은 4시간 후에 혈액 glucose의 8%였다.

• Reproductive and Developmental Biology
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• 제28권2호
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• pp.95-99
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• 2004

본 연구는 미성숙돼지 난모세포의 체외 성숙에 있어서 myo-inositol의 영향을 알아보기 위하여 실시하였다. 미성숙 돼지 난모세포을 myo-inositol을 포함하는 또는 포함하지 않은 체외 성숙배지에서 44시간 체외 성숙을 유도하였을 때 성숙율은 myo-inositol을 첨가한 성숙배지에서 성숙시킨 실험구에 유의하게 높았다(P<0.05). Myo-inositol 에 의한 성숙율 향상에 있어서 난구세포의 영향을 알아보기 위하여 난구세포의 치밀도에 따라 분류하여 미성숙 난모세포을 myo-inositol을 포함하는 체외 성숙배지에서 배양하였을 때 난구세포가 치밀한 미성숙 난모세포에서가 더 높은 성숙율을 나타내었다(P<0.05). 그러나 난구세포가 치밀하지 않은 미성숙 난모세포도 myo-inositol을 포함하는 성숙배지에서 배양하면 성숙율은 대조구에 비하여 유의하게 높았다(P<0.05). 이러한 결과는 돼지 난모세포의 체외 성숙배지에 myo-inositol의 첨가는 체외 성숙율을 향상시킬 수 있음을 나타내고 있다.

• 환경위생공학
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• 제18권2호
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• pp.60-66
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• 2003

This study was carried out to elucidate the preventive mechanism of molybdenum on lead-induced neuropathy, An animal model of lead neuropathy was induced by feeding diet containing lead to Sprague-Dawley rat for three weeks. Four weeks aged Sprague-Dawley rats were divided into four groups : normal control group, 10ppm-lead treated group, 1mg/kg-molybdenum treated group, 10ppm-lead and 1mg/kg-molybdenum treated group. The parameters on neuropathy were examined by measuring concentration of myo-inositol and myo-inosito uptake in sciatic nerve. In the lead-treated rats, myo-inositol concentration and myo-inositol uptake rate were reduced by from 54% to 33% respectively. This deficit results from that myo-inositol uptake system which is carrier mediated and sodium-potassium dependent was inhibited by the lead treatment. However, the molybdenum administration significantly eliminated the impairment and maintained myo-inositol concentration to about 82% of normal level. These results suggest that lead-induced neurotoxicity was significantly reduced by administration of molybdenum and the mechanism might be partly normalization of myo-inositol uptake system in sciatic nerve.

• 대한치과보철학회지
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• 제18권1호
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• pp.73-86
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• 1980

Recently, the controversy continues as to whether maximum intercuspation of teeth should occur at the terminal hinge position(the condylar theory) or at the myo-co(the neuromuscular theory). There is also much controversy regarding the antero-posterior position of myo-co. The object of this study was to measure and compare with the positional relations of centric relation, centric occlusion and myo-co, and free-way space using Mandibular Kinesiograph and Myo-monitor in the 40 subjects without stomatognathic problems. Mandibular Kinesiograph(M.K.G.) was originally conceived as a research instrument to track mandibular movement and position. As its use in research progressed, its great diagnostic value became apparent in case by case. And Myo-monitor was developed as a means of applying the neuromuscular approach to occlusion. Thus the Myo-monitor technique is an intra-systemic approach to occlusal positioning using patient's own musculature, and Myo-monitor is used to relax the musculature by a light myopulse induced electronically. From this experiment, the following results were obtained. 1. The adaptive free-way space before muscle relaxation was an average of $1.6{\pm}60mm$, and the true free-way space after muscle relaxation using Myo-monitor was an average of $2.4{\pm}0.74mm$. 2. It took an average of $25{\pm}3.11$ minutes to relax the mandibular musculature by Myo-monitor and administration of 5mg. Diazepam and an average of $38{\pm}4.73$ minutes by Myo-monitor without administration of Diazepam. 3. Myo-co existed anterior to centric occlusion, with an average of $0.53{\pm}0.31$ mm, and centric relation existed posterior to centric occlusion, with an average of $0.57{\pm}0.58mm$ before muscle relaxation and with an average of $0.57{\pm}0.43mm$ after muscle relaxation. 4. Centric relation coincided with centric occlusion in 5 of 40 subjects(12.5%), and posterior to centric occlusion in the rest of cases (87.5%). 5. Myo-co existed anterior to centric occlusion in 38 of 40 subjects(95%), except 1 subject that coincided with centric occlusion and 1 subject that existed posterior to centric occlusion. 6. Myo-co and centric relation existed inferior to centric occlusion and the lateral displacement was various with individual difference. 7. The total displacement from centric occlusion to centric relation was an average of $0.74{\pm}0.64mm$ before muscle relaxation, and an average of $0.68{\pm}0.53mm$ after muscle relaxation, and the total displacement from centric occlusion to myo-co was an average of $1.07{\pm}0.58mm$.

• Asian-Australasian Journal of Animal Sciences
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• 제12권5호
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• pp.689-694
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• 1999

This experiment was carried out to isolate the partial bovine (Bos Taurus coreanae) myogenic factor encoding gene, MyoD, using the rat myogenic factor (MyoD) gene sequence and to compare the gene sequence between another myogenic factor (Myf 5) and MyoD gene of the bovine. To make the probe and isolate the MyoD gene, PCR was performed to amplify rat and bovine MyoD gene including exon I, II and intron I. The homology between mouse and bovine MyoD is high; bovine MyoD gene shows 17 different gene sequence region compared to rat MyoD. Among those, two regions have significant differences; one is the exon I part between 2834 and 2850 bp, the other is intron part between 3274 and 3303 bp of the mouse. At this region homology was 40% in the former and 50% in the latter. Homology between bovine MyoD and Myf5 was 83% in the exon 1. Especially exon I in the Myf5 602-617 bp and 651-683 bp have significant differences. These results suggest that MyoD gene have a similar gene structure in mouse and bovine and MyoD and Myf5 of the bovine, at least in part, have a similar expression and activity.

• 건축역사연구
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• 제22권4호
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• pp.73-82
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• 2013

This study aims to find architectural characteristic of Nam-kwan-wang-myo as known as Nam-myo, Especially focusing on difference between before and after it's reconstruction in 1899. Nam-kwan-wang-myo is a shrine for Kwan-woo who was warlord in ancient china. Belief of Kwan-Woo was introduced to Korea in Japanese invasion of 1592 and the shrine was built in 1598. Belief of Kwan-Woo diffused for the late Joseon, during the reign of Gojong, many people have faith in Kwan-Woo including the king. There was four Kwan-wang-myo around the Hanyang at that time. In 1899 a fire of unknown cause broke out at Nam-kwan-wang-myo, so the main buildings burned down. The king instructed reconstruction of the shrine even though there was in financial difficulties, it had done in the midst of a national crisis. The buildings almost restored as before. The buildings in the shrine has strong characteristics of Chinese architecture because it made by people of the Ming dynasty. Two side-by-side roofs, accumulated brick exterior are important architectural feature, but also all the buildings in the mail hall area Surrounded by the closed-connected fence is hard to find examples in Korea traditional architecture. And Nam-kwan-wang-myo just had maintained architectural characteristics including layout of buildings, shape of the each building until it's reconstruction(1899).

• Journal of Oral Medicine and Pain
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• 제14권1호
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• pp.89-103
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• 1989

In order to evaluate the influences of Myo-monitoring on masticatory muscles, Myo-monitoring on 31 normal persons and 30 persons with one more temporomandibular dysfunction symptoms during 45 minutes or above. The author observed velocities of mandibular opening and closing movement, variabilities of mandibular rest position and EMG activities of temporal and masseter muscles. The obtained results were as follows : 1. There were no significant differences on velocities of mandibular opening and closing movement between before and after Myo-monitoring. 2. There were significant differences on vertical dimension and total dimension form mandibular rest position to centric occlusion between before and after Myo-monitoring but no significant differences on anteroposterior and lateral dimension. 3. Activities of temporal and masseter muscles were decreased in Myo-Monitoring. 4. There were disappeared significant differences on EMG activity values between normal and symptom groups after myo-monitoring.

• 생명과학회지
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• 제21권8호
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• pp.1076-1082
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• 2011

미세소관(microtubule) 위를 이동하는 키네신은 분비소포를 이동시키는 운동단백질이다. KIF5s (KIF5A, KIF5B and KIF5C)는 세포막으로 싸인 각종 세포 내 소기관과 결합하여 미세소관을 따라 목적지까지 이동시킨다는 결과는 알려져 있지만, 어떻게 상대의 cargo를 인식하는지는 밝혀지지 않았다. 본 연구는 KIF5B의 결합 단백질을 동정하기 위하여 효모 two-hybrid system을 사용하여 KIF5B와 특이적으로 결합하는 Myo9b을 확인하였다. Myo9b는 액틴위를 이동하는 운동단백질로 다른 KIF5s들과도 결합함을 효모 two-hybrid assay로 확인하였다. 또한 Myo9s의 GTPase 활성화 단백질(GAP) 영역은 KIF5B와 결합하는데 필수영역임을 확인하였고, 이러한 단백질간의 결합은 Glutathione S-transferase (GST) pull-down assay를 통하여서도 확인하였다. 생쥐의 뇌 파쇄액에 KIF5B들의 항체로 면역침강을 행하여 Myo9s 단백질을 확인한 결과, KIF5s는 Myo9s 단백질과 특이적으로 함께 침강하였다. 이러한 결과들은 kinesin-I는 액틴 결합 운동단백질과 직접 결합함을 보여준다.

• 환경위생공학
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• 제11권2호
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• pp.21-26
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• 1996

In our previous studies, we reported that lead intoxicated nerve cell by inhibition of the Na$^{+}$-K$^{+}$ ATPase activity and reduction of myo-inositol in nerve cell. As the second series of experiments, in order to understand toxic mechanism of lead for nerve cell, the characteristics of myo-inositol transport system and the effect of lead on its system have been studied in the sciatic nerves of control and lead-treated rats. A lead intoxicated animal model was induced by feeding diet containing lead to Sprague-Dawley rat for two weeks. Four weeks aged Sprague-Dawley rats were divided into three group : normal control group, 10ppm-lead treated group, 100ppm-lead treated group. All rats were sacrified at the end of two weeks. The rate o myo-inositol transport by sciatic nerve isolated from lead-treated rat was significantly decreased compared with that of control rat. This deficit results from that myo-inositol transport system which is carrier mediated and sodium-potassium dependent was inhibited by the lead treatment (both 10ppm and 100ppm) due to increase of the Km value without affecting Vmax value for myo-inositol carrier. These observations suggest that the toxic mechanism of lead on nerve myo-inositol transport system might be a change of affinity without change of maximum transport velocity for carrier.

• The Korean Journal of Physiology and Pharmacology
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• 제20권2호
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• pp.139-145
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• 2016

Previously characterized as a backward motor, myosin VI (MYO6), which belongs to myosin family, moves toward the minus end of the actin track, a direction opposite to all other known myosin members. Recent researches have illuminated the role of MYO6 in human cancers, particularly in prostate cancer. However, the role of MYO6 in glioma has not yet been determined. In this study, to explore the role of MYO6 in human glioma, lentivirus-delivered short hairpin RNA (shRNA) targeting MYO6 was designed to stably down-regulate its endogenous expression in glioblastoma cells U251. Knockdown of MYO6 significantly inhibited viability and proliferation of U251 cells in vitro. Moreover, the cell cycle of U251 cells was arrested at G0/G1 phase with the absence of MYO6, which could contribute to the suppression of cell proliferation. In conclusion, we firstly identified the crucial involvement of MYO6 in human glioma. The inhibition of MYO6 by shRNA might be a potential therapeutic method in human glioma.