• 제목/요약/키워드: MTT method.

검색결과 423건 처리시간 0.029초

Methanolic Extract of Plumbago Zeylanica - A Remarkable Antibacterial Agent Against Many Human and Agricultural Pathogens

  • Singh, Mukesh Kumar;Pandey, Ajit;Sawarkar, Hemant;Gupta, Anshita;Gidwani, Bina;Dhongade, Hemant;Tripathi, Dulal Krishna
    • 대한약침학회지
    • /
    • 제20권1호
    • /
    • pp.18-22
    • /
    • 2017
  • Objectives: The current investigation was carried out to determine the cytotoxic and the antimicrobial activities of methanolic extracts of Plumbago zeylanica. Methods: The stems, leaves, and whole plants were air dried and extracted with methanol by using a Soxhlet extractor for 72 hours at $55-60^{\circ}C$. The antimicrobial activities were determined from the zones of inhibition, which were measured by using the agar well diffusion method, and the cytotoxicity assays were performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. Results: The methanolic extracts of the stem and the leaves of Plumbago zeylanica were tested against six bacterial species and nine fungal species, and both extracts showed antimicrobial activity in a dose-dependent manner. The leaf extract of Plumbago zeylanica showed maximum antimicrobial activity against both Staphylococcus aureus sub sp aureus and Fusarium oxysporum. The stem extract was found to be more antimicrobial against the Pseudomonas aeruginosa and the Penicillium expansum species. MTT assays were used to test the cytotoxicity of the whole plant extract in the HCT-116 and the K-562 cell lines, and that extract was shown to have weak cytotoxicity in both cell lines. Conclusion: In the present study, the methanolic stem extracts of Plumbago zeylanica were found to possess remarkable antibacterial activities against many human and agricultural pathogens. The extracts were also found to possess significant antifungal activities, but the antifungal activities were less than the antibacterial activities. Finally, the extracts were found to have weak cytotoxicities in the HCT-116 and the K-562 cell lines.

Amaranth의 Squalene 동정과 잔사 추출물의 항암 작용 검색 (Isolation and Identification of Squalene and Antineoplastic Activity of Its Residue Extract in Amaranth)

  • 이재학;문형인;이정일;강철환;이승택
    • 한국작물학회지
    • /
    • 제41권4호
    • /
    • pp.450-455
    • /
    • 1996
  • 국내에서 종실용 amaranth의 이용 가능성을 조사하기 위한 목적으로 종실에서 squalene을 분리, 동정하였고, squalene 추출후 부산물의 활용방안으로 그 부산물의 항암활성검색을 한 결과를 요약하면 다음과 같다. 1. Amaranth 종실에서 추출, 분리한 squalene 함량은 0.43%이고, GC /Mass spectrum으로 확인한 결과 99%의 순도를 나타내었으며, 분자구조식은 잠정적으로 동물성 squalene과 동일한 것으로 나타났다. 2. MTT 방법을 이용한 인간의 위암, 대장암 세포주에 대한 항암활성효과를 검색한 결과 부산물의 조추출물은 230$\mu\textrm{g}$/m1보다 낮은 농도에서 유의한 항암효과를 보였다.

  • PDF

포공령의 카드뮴에 대한 세포독성 억제효과 (The Inhibitory Effects of Taraxaci Herba against Cadmium induced Cytotoxicity)

  • 한두석;이기남;이종섭;백승화
    • 약학회지
    • /
    • 제42권3호
    • /
    • pp.307-311
    • /
    • 1998
  • This study was carried out to evaluate antitoxic effects Taraxaci Herba extract against Cadium by calorimetric methods. The antitoxic activity of Taraxaci Herba ex tract in NIH 3T3 fibroblasts was evaluated by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-phenyl-2H-tetrazoliumbromide), NR (Neutral red) and SRB (Sulforhodamine B protein) assay. The light microscopic study was carried out to observe morphological changes of the treated cells. These results were obtained as follows; The concentration of $10^{-2}mg/ml$ of Taraxaci Herba extract was shown significant antitoxic activity. The number of NIH 3T3 fibroblasts were antitoxic and tend to regenerate. These results suggest that Taraxaci Herba extract retains a potential antitoxic activity.

  • PDF

복합모델 다차량 추종 기법을 이용한 차량 주행 제어 (Vehicle Cruise Control with a Multi-model Multi-target Tracking Algorithm)

  • 문일기;이경수
    • 대한기계학회:학술대회논문집
    • /
    • 대한기계학회 2004년도 추계학술대회
    • /
    • pp.696-701
    • /
    • 2004
  • A vehicle cruise control algorithm using an Interacting Multiple Model (IMM)-based Multi-Target Tracking (MTT) method has been presented in this paper. The vehicle cruise control algorithm consists of three parts; track estimator using IMM-Probabilistic Data Association Filter (PDAF), a primary target vehicle determination algorithm and a single-target adaptive cruise control algorithm. Three motion models; uniform motion, lane-change motion and acceleration motion, have been adopted to distinguish large lateral motions from longitudinal motions. The models have been validated using simulated and experimental data. The improvement in the state estimation performance when using three models is verified in target tracking simulations. The performance and safety benefits of a multi-model-based MTT-ACC system is investigated via simulations using real driving radar sensor data. These simulations show system response that is more realistic and reflective of actual human driving behavior.

  • PDF

In vitro test method for efficacy evaluation on whitening cosmetics

  • Whang, Kyu-Wang
    • 대한화장품학회지
    • /
    • 제28권3호
    • /
    • pp.41-62
    • /
    • 2002
  • 여러 종류의 미백제들이 한국에서 보고되고 있으나 표준화된 유효성 protocol은 아직 확립되지 않은 실정이다. 그러므로 경제적이고 재현성있는 표준화된 유효성방법이 필요하다. 비방사선표지된 멜라닌정량법은 멜라닌형성에 대한 정량법으로 좋은 방법이 될 수 있으며 정상적인 사람의 melanocyte를 이용한 MTT assay는 세포의 proliferation을 정량하기 위한 좋은 방법에 될 수 있다.

수산물 및 수산 발효식품의 암세포 억제효과 (Anticancer Effect of Extracts from the Marine and Salted Fish Products.)

  • 임현수;김수현;유은정;강동수;최명락;송상호
    • 생명과학회지
    • /
    • 제11권1호
    • /
    • pp.48-53
    • /
    • 2001
  • This study was performed to observe the cytotoxic effect of the various salted fish extracts against cancer cell line, human hepatocellular carcinoma (HepG2) using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) method. Urechis unicinctus was the strongest cytotoxic effects among any other traditional salted fish products. The growth inhibition ratio of Urechis unicinctus hot-water extracts was 94.5% at the concentration of 1000$\mu\textrm{g}$/$m\ell$. On the other hand, in case of salted fish methanol extracts, salt-fermented shad gizzard was showed the strongest cytotoxic effects. The growth inhibition ratio of salt-fermented shad gizzard methanol extracts was investigated 90% at the concentration of 1000$\mu\textrm{g}$/.$m\ell$.

  • PDF

Microfluidic Image Cytometry (μFIC) Assessments of Silver Nanoparticle Cytotoxicity

  • Park, Jonghoon;Yoon, Tae Hyun
    • Bulletin of the Korean Chemical Society
    • /
    • 제33권12호
    • /
    • pp.4023-4027
    • /
    • 2012
  • Cytotoxicity assessment of silver nanoparticles (AgNPs) was performed using MTT-based microfluidic image cytometry (${\mu}FIC$). The $LC_{50}$ value of HeLa cells exposed to AgNPs in the microfluidic device was estimated as 46.7 mg/L, which is similar to that estimated by MTT-based IC for cells cultured in a 96 well plate (49.9 mg/L). These results confirm that the ${\mu}FIC$ approach can produce cytotoxicity data that is reasonably well-matched with that of the conventional 96 well plate system with much higher efficiency. This ${\mu}FIC$ method provides many benefits including ease of use and low cost, and is a more rapid in vitro cell based assay for AgNPs. This may aid in speeding up data acquisition in the field of nanosafety and make a significant contribution to the quantitative understanding of nanoproperty-toxicity relationships.

다차량 추종 적응순항제어 (Multi-Vehicle Tracking Adaptive Cruise Control)

  • 문일기;이경수
    • 대한기계학회논문집A
    • /
    • 제29권1호
    • /
    • pp.139-144
    • /
    • 2005
  • A vehicle cruise control algorithm using an Interacting Multiple Model (IMM)-based Multi-Target Tracking (MTT) method has been presented in this paper. The vehicle cruise control algorithm consists of three parts; track estimator using IMM-Probabilistic Data Association Filter (PDAF), a primary target vehicle determination algorithm and a single-target adaptive cruise control algorithm. Three motion models; uniform motion, lane-change motion and acceleration motion. have been adopted to distinguish large lateral motions from longitudinal motions. The models have been validated using simulated and experimental data. The improvement in the state estimation performance when using three models is verified in target tracking simulations. The performance and safety benefits of a multi-model-based MTT-ACC system is investigated via simulations using real driving radar sensor data. These simulations show system response that is more realistic and reflective of actual human driving behavior.

Fabrication of Cross-linked Nano-Fibrous Chitosan Membranes and Their Biocompatibility Evaluation

  • Nguyen, Thi-Hiep;Lee, Seong-Jin;Min, Young-Ki;Lee, Byong-Taek
    • 한국재료학회지
    • /
    • 제21권2호
    • /
    • pp.125-132
    • /
    • 2011
  • Fibrous chitosan membranes were fabricated as a substrate for skin applications using an electro-spinning process with different solvents and varying concentrations. Scanning electron microscopy (SEM) images confirmed that the formation of the chitosan fibrous membrane in trifluoroacetic acid was better than that in acetic acid. Fourier transform infrared spectroscopy showed that the chitosan fibers were cross-linked with glutaraldehyde, and that the cytotoxicity of the aldehyde groups was reduced by glycine and washing by NaOH and DI water. Chitosan cross-linked fibrous membranes were insoluble in water and could be washed thoroughly to wash away glycine and excess NaOH and prevent the infiltration of other water soluble bio-toxic agents using DI water. MTT assay method was employed to test the cytotoxicity of chitosan membranes during fabricating, treating and washing processes. After the dehydration of cell cultured chitosan membranes, cell attachment behavior on the material was evaluated using SEM method. Effect of the treatment processes on the biocompatibility of the chitosan membranes was shown by comparing of filopodium and lamellipodium of fibroblast cells on grown washed and unwashed chitosan fibrous membrane. The MTT assay and SEM morphology confirmed that the washed chitosan fibrous membrane increased cell attachment and cell growth, and decreased toxicity compared to results for the unwashed chitosan fibrous membrane.

Enhanced Hemolytic Biocompatibility of Hydroxyapatite by Chromium (Cr3+) Doping in Hydroxyapatite Nanoparticles Synthesized by Solution Combustion Method

  • Bandgar, Sneha S.;Yadav, Hemraj M.;Shirguppikar, Shailesh S.;Shinde, Mahesh A;Shejawal, Rajendra V.;Kolekar, Tanaji V.;Bamane, Sambhaji R.
    • 한국세라믹학회지
    • /
    • 제54권2호
    • /
    • pp.158-166
    • /
    • 2017
  • For the better success of biomedical implant surgery, we used a modified solution combustion method to synthesize Hydroxyapatite (HA) and Chromium ($Cr^{3+}$) modified Cr-HA with different concentrations of 0.5, 1.0, 1.5, 2.0 and 2.5. The Cr-HA nanopowder was characterized by TGA, XRD, SEM-EDS and TEM. The HA and Cr-HA powders were subjected to in vitro biological studies to determine their biocompatibility and hemocompatibility. The cytotoxicity of HA and Cr-HA were evaluated on Hela (Cervical cancer) cells and L929 (mouse fibroblast) cells by using MTT assay. Hemocompatibility studies demonstrated a noticeable haemolytic ratio below 5%, which confirms that these materials are compatible in nature with human blood. The results of the present work confirm that the synthesised HA and Cr-HA are biocompatible and can be extensively used in the biomedical field to improve overall material biological properties.