• 대한치과보존학회:학술대회논문집
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• 대한치과보존학회 2002년도 추계학술대회
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• pp.698-698
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• 2002

No Abstract, See Full Text

• Bulletin of the Korean Chemical Society
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• 제31권6호
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• pp.1463-1464
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• 2010

• Journal of Applied Biological Chemistry
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• 제61권1호
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• pp.9-15
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• 2018

우리는 황련에서 분리된 베르베린의 화장품소재로의 활성을 평가하기 위해 항산화와 항주름 효능을 분석하였다. 시료의 독성을 평가하기 위해 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay를 수행하였으며 항산화와 항주름 활성평가를 위해 ROS 생성능, hyaluronic acid 생성능, MMP-2, -9, TIMP-1,-2, $TNF-{\alpha}$의 발현을 각각 측정하였다. 베르베린에 대한 자외선의 세포 독성은 CCD-986sk 섬유아세포를 이용하여 MTT 분석으로 측정되었고, 세포 독성이 $25{\mu}g/mL$의 농도 미만에서 관찰되지 않았다. 또한 베르베린이 ROS 생산을 농도 의존적으로 감소시키고 히알루론산의 합성을 촉진한다는 것을 발견했다. 베르베린은 주름 형성과 관련된 MMP-2 및 MMP-9의 protein 단계 및 mRNA 발현을 감소시키고 TIMP-1 및 TIMP-2의 발현을 증가시켰다. 프로 pro-inflammatory cytokine 으로 알려진 $TNF-{\alpha}$에 대한 베르베린의 억제효능을 실험한 결과, 농도의존적으로 $TNF-{\alpha}$ 유전자를 억제하는 효과를 나타내었다. 이러한 결과를 통해 베르베린은 활성산소종의 억제와 TIMP-1과 -2의 발현 조절에 의한 MMP-2,-9의 감소로 항산화 및 항주름에 효과가 있는 화장품 소재로서의 가치를 가진다고 사료된다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제13권4호
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• pp.391-404
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• 1991

Three anticncer agents which are different in time or dosage dependence as well as in phase specificity, namely mitomycin and adriamycin from natural products, and widely different cancer cell lines_Four epidermoid carcinomas originated from larynx, cervix, skin and gut were used toghether with one osteosarcoma as the target cell of single and combined administration of anticancer drugs. Semiautomated tetrazolium dye assay(MTT) appears to offer an attractive option for chemosensitivity of head and neck cancers since it is a simple, valid and inexpensive method of assessing chemosensitivity for large samples in a short time. The results obtained form this study were as follows. 1. Good correlations were obtained with the results of the MTT test and those of $^3H$ thymidine uptake assay. 2. $LD_{50}$ values of HIST and St.Ca. which showed relatively high doubling time on adriamycin were $30{\mu}g/ml$ and $15{\mu}g/ml$ while those of HeLa, Hep-2 and KHOS/NP were $2.1{\mu}g/ml$, $4.8{\mu}g/ml$, and $6.8{\mu}g/ml$ respectively. 3. The $LD_{50}$ value of 5-FU on five cancer cells were very high ranging from 15mg/ml to almost indefinite number, which means 5-FU is very resistant to epidermoid carcinomas or osteosarcoma examined in this study. 4. Mitomycin was relatively effective showing 80% cancer killing effect on HeLa, 70% on St. Ca. and 50% on Hep-2 at the high concentrations used. 5. Adriamycin was the most effective showing 90% cancer cell killing effect on KHOS/NP, 98% on HeLa, 80% both on Hep-2 and St. Ca. The least susceptible cancer cells toward adriamycin was HIST having only 55% cell killing effect at the high cincentration. 6. Combined therapy of adriamycin and 5-FU was more effective than single administration in all the cases examined. Most effective synergism was observed on St. Ca. at the low concentration, showing 21 times higher than each single administration.

• Applied Biological Chemistry
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• 제47권2호
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• pp.202-207
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• 2004

어성초 분말을 포함하는 차가버섯 발효 조성물의 물 추출물이 인체 위암 세포 AGS 및 대장암 세포 HCT-15, 그리고 정상세포 NIH3T3 fibroblast의 세포 생육에 미치는 영향을 검토하였다. 세포독성 실험은 세포수 count와 MTT 방법으로 측정하였다. 어성초 분말을 포함하는 차가버섯 발효 조성물의 제조는 차가버섯과 어성초 분말을 혼합하고, 여기에 대두 발효 미생물원을 다시 혼합시켜 습도 $50{\sim}60%$,온도 $30{\sim}37^{\circ}C$에서 30일 정도 발효시킨 후 건조 시킨 분말에서 5% 물 추출물을 얻어 동결건조 시켜 실험에 제공하였다. MTT assay 방법에서 차가버섯 발효 조성물의 수용성 추출물 0.16, 0.4, 0.8, 1.6 및 4.0 mg/ml 첨가 농도에서 AGS 세포 생육은 13, 25, 40, 67 및78% 억제 되었으며, HCT-15세포 생육은 22, 40, 50, 69및 76%씩 각각 억제되었다. 그러나 동일한 실험조건에서 정상 세포수 NIH3T3은 86% 이상의 생존율을 나타내었다. 차가버섯 발효 조성물의 수용성 추출물은 인체 대장암 세포주 HCT-15와 위암 세포수 AGS에 대해 생육억제 작용이 강한 반면, 정상세포 NIH3T3에 대해서는 세포 독성을 거의 나타내지 않아 가장 바람직한 암예방 또는 항암식품 개발가능성을 제시하였다.

• 한국버섯학회지
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• 제17권2호
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• pp.70-77
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• 2019

국내 자생하는 야생버섯의 생리활성 및 항염 활성을 분석하기 위하여 버섯추출물에 대한 DPPH 라디컬 소거능, 아질산염 소거능, 총 폴리페놀 함량, NO 생성저해도 및 세포독성을 분석하였다. 국내 수집 야생버섯 중에서 DPPH 라디컬 소거능이 높은 버섯은 64.2%를 나타낸 박막깔때기버섯(OK825)과 69.7%를 보인 흰우단버섯(OK829)이었다. 아질산염 소거능은 흰우단버섯(OK811)이 64.1%로 가장 높았다. 야생수집 버섯류 중에서 가장 높은 총 폴리페놀 함량을 나타낸 것은 젖비단버섯(OK804), 흰우단버섯(OK829) 및 큰밤갈색광대버섯(OK944)으로 각각 19.7 mg GAE/g, 20.2 mg GAE/g, 22.3 mg GAE/g의 값을 보였다. NO 생성저해도의 경우, 박막깔때기버섯(OK825)과 노란다발버섯(OK826)은 각각 11.8%와 11.2%로 가장 낮은 NO를 생성하는 것으로 나타나 수집 버섯 추출물 중에서 가장 높은 항염증 효능을 보였다. MTT를 이용한 세포생존율(%)을 측정한 결과 젖비단그물버섯(OK804), 흰우단버섯(OK811), 노란젖버섯(OK904)의 추출물에서는 각각 40.3%, 48.3%, 43.2%로 가장 높은 생존율을 나타내었다. 본 연구결과는 국내 버섯산업의 확대를 위하여 우수버섯자원을 발굴함과 동시에 토종 버섯자원을 이용한 새로운 천연물 유래 생리활성 물질로 활용하기 위한 기초자료로 활용도가 높으리라 기대된다.

• Toxicological Research
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• 제13권1_2호
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• pp.87-94
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• 1997

This study was carried out to investigate toxicity of insecticide carbofuran and compensatory effects of phenobarbital sodium (PB) in vivo and in vitro. Sprague Dawley male rats were used as experimental animals and divided into carbofuran only administered group and simultaneous application group of carbofuran and PB. At 30 rain and 1, 3, 6, 12, 24, 48 and 96 hrs after each treatment, the animals were sacrificed by decapitation. Kidney were immediately removed, immersed in fixatives, and processed with routine method for light microscopic study. Paraffin sections were stained with H-E, PAM and PAS. $5.0\times 10^4$ cell/ml of NIH 3T3 fibroblast in each well of 24 multidish were cultured: After 24 hours, the cells were treated with solution of six groups; control group cultured in media only, carbofuran $MTT_50$ or $NR_50$ group cultured in the media containing carbofuran $MTT_50$ or $NR_50$ and four experimental groups cultured in the media containing carbofuran $NR_50$ plus various concentratins of PB. After the NIH 3T3 fibroblast of all groups were cultured in same condition for 48 hours, Tetrazolium MTT (MTT) and NR (neutral red) assay were performed to evaluate the cytotoxicity of cell organelles. Under the light microscope, atrophic change of renal corpuscles were frequently observed in 1 and 2 days after carbofuran treatment. The increase of the mesangium was apparent in 1 and 2 days after carbofuran treatment. Necrotic changes of the epithelium and loss of brush border of proximal tubules were most severe at 2 and 3 days after carbofuran treatment, respectively. In contrast, there were no evidences of the toxic effects on renal tissues at 48hrs in carbofuran-PB treated groups. Carbofuran $MTT_50$ and $NR_50$ were 78$\mu M$, 82.5$\mu M$ respectively. MTT and NR quantities were significantly increased in carbofuran-PB 100$\mu M$ treatment group and carbofuran-PB 100$\mu M$ treatment group. On the basis of these results, it is obvious that PB has compensatory effects against carbofuran toxicity.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.453-456
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• 2001

배양된 대황으로 모상근으로부터 추출한 물질에 대한 분획별 신장상피세포에 있어서의 세포 독성을 조사하였다. l 수층과 클로로포름 층으로부터 얻은 대황 모상근 추출물모두 농도의 증가에 따라 세포에 미치는 독성이 증가하였다. 2. 클로로포름층으로부터 얻은 대황 모상근 추출물이 수층으로부터 얻은 대황 추출물보다 세포에 미치는 독성이 크게 나타났다. 콜로로포름층 분획의 $MTT_{50}$, $NR_{50}$, $SRB_{50}$은 각각 289.3 ${\mu}g/ml$. 302.7 ${\mu}g/ml$. 433.8 ${\mu}g/ml$ 이었고, 수층 분획물의 $MTT_{50}$, $NR_{50}$, $SRB_{50}$은 각각 475.8 ${\mu}g/ml$. 428.3 ${\mu}g/ml$. 549.5 ${\mu}g/ml$ 이었다. 3. 세포독성 측정방법에 따라 차이를 보였으며 수층 분획의 경우 NR정량법에서 클로로포름 층 분획의 경우 MTT정량법에서 독성 정도가 더 높게 나타났다.

• Biomolecules & Therapeutics
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• 제20권6호
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• pp.562-568
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• 2012

Cytochrome P450 BM3 (CYP102A1) from Bacillus megaterium is a self-sufficient monooxygenase that consists of a heme domain and FAD/FMN-containing reductase domain (BMR). In this report, the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) by BMR was evaluated as a method for monitoring BMR activity. The electron transfer proceeds from NADPH to BMR and then to BMR substrates, MTT and CTC. MTT and CTC are monotetrazolium salts that form formazans upon reduction. The reduction of MTT and CTC followed classical Michaelis-Menten kinetics ($k_{cat}=4120\;min^{-1}$, $K_m=77{\mu}M$ for MTT and $k_{cat}=6580\;min^{-1}$, $K_m=51{\mu}M$ for CTC). Our continuous assay using MTT and CTC allows the simple, rapid measurement of BMR activity. The BMR was able to metabolize mitomycin C and doxorubicin, which are anticancer drug substrates for CPR, producing the same metabolites as those produced by CPR. Moreover, the BMR was able to interact with CYP1A2 and transfer electrons to promote the oxidation reactions of substrates by CYP1A2 and CYP2E1 in humans. The results of this study suggest the possibility of the utilization of BMR as a surrogate for mammalian CPR.

• Archives of Plastic Surgery
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• 제32권5호
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• pp.625-635
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• 2005

In this study, the effects of verapamil on growth rate, apoptosis, production of transforming growth factor (TGF-${\beta}$) and fibronectin were evaluated in keloid and normal human dermal fibroblasts. Both fibroblasts were primarily cultured from earlobe keloids of three female patients and treated with various concentrations of verapamil. Cell toxicity was assessed by MTT assay, growth rate and apoptosis by FACS, and the production of TGF-${\beta}$ and fibronectin by ELISA and Western blot, respectively. In the $MTT_{50}$, the cell growth was more suppressed in keloid fibroblasts. In the $MTT_{90}$, cell growth was more stimulated in normal fibroblasts. No significant effect appeared on TGF-${\beta}$ expression but an increase in extracellular fibronectin secretion was found in keloid fibroblasts. Keloid fibroblasts responded to verapamil more sensitively, and the percentage of apoptosis was higher at the $MTT_{50}$l. In brief, verapamil had growth-inhibitory effect with inducing apoptosis at the $MTT_{50}$, but rather growth-stimulatory effect at the $MTT_{90}$. The biphasic effect of verapamil depending on the dose might explain one of the reasons of relapse after keloid treatment with verapamil. Clinical application with high concentration (2.5 mg/ml) is advised unless excessive dosage is used.