Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Physiological activities of extracts of wild mushrooms collected in Korea

국내 야생수집 버섯류 추출물의 생리활성 분석

  • An, Gi-Hong (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Jae-Han (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Lee, Kang-Hyo (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Han, Jae-Gu (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA)
  • 안기홍 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 조재한 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 이강효 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 한재구 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과)
  • Received : 2019.05.13
  • Accepted : 2019.06.20
  • Published : 2019.06.30
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Abstract

This study was carried out to analyze the physiological activities of wild mushroom extracts collected from the Gangwon-do, Gyeonggi-do, and Chungcheongbuk-do provinces in Korea. Among the wild mushroom extracts, those of Clitocybe robusta and Leucopaxillus giganteus (OK829) showed the highest DPPH radical scavenging activities. Nitrite scavenging activity of the L. giganteus extract (OK811) was determined to be 64.1%, which is considerably higher than those of the other mushroom extracts analyzed in this study. The total polyphenol levels in Suillus granulatus, L. giganteus (OK829), and Amanita manginiana extracts were found to be 19.7 mg GAE/g, 20.2 mg GAE/g, and 22.3 mg GAE/g, respectively. To determine their anti-inflammatory effects, nitric oxide production, and cell viability, NO measurement and MTT assays were performed using lipopolysaccharide (LPS)-treated RAW 264.7 cells. The levels of nitric oxide (NO) produced by the C. robusta and Hypholoma fasciculare extracts were remarkably lower than those produced by the others. In our MTT assay, the extracts of S. granulatus, L. giganteus (OK811), and Lactarius chrysorrheus showed high cell viabilities of 40.3%, 48.3%, and 43.2%, respectively. These results can provide the fundamental data for extracting useful compounds from wild mushrooms.

국내 자생하는 야생버섯의 생리활성 및 항염 활성을 분석하기 위하여 버섯추출물에 대한 DPPH 라디컬 소거능, 아질산염 소거능, 총 폴리페놀 함량, NO 생성저해도 및 세포독성을 분석하였다. 국내 수집 야생버섯 중에서 DPPH 라디컬 소거능이 높은 버섯은 64.2%를 나타낸 박막깔때기버섯(OK825)과 69.7%를 보인 흰우단버섯(OK829)이었다. 아질산염 소거능은 흰우단버섯(OK811)이 64.1%로 가장 높았다. 야생수집 버섯류 중에서 가장 높은 총 폴리페놀 함량을 나타낸 것은 젖비단버섯(OK804), 흰우단버섯(OK829) 및 큰밤갈색광대버섯(OK944)으로 각각 19.7 mg GAE/g, 20.2 mg GAE/g, 22.3 mg GAE/g의 값을 보였다. NO 생성저해도의 경우, 박막깔때기버섯(OK825)과 노란다발버섯(OK826)은 각각 11.8%와 11.2%로 가장 낮은 NO를 생성하는 것으로 나타나 수집 버섯 추출물 중에서 가장 높은 항염증 효능을 보였다. MTT를 이용한 세포생존율(%)을 측정한 결과 젖비단그물버섯(OK804), 흰우단버섯(OK811), 노란젖버섯(OK904)의 추출물에서는 각각 40.3%, 48.3%, 43.2%로 가장 높은 생존율을 나타내었다. 본 연구결과는 국내 버섯산업의 확대를 위하여 우수버섯자원을 발굴함과 동시에 토종 버섯자원을 이용한 새로운 천연물 유래 생리활성 물질로 활용하기 위한 기초자료로 활용도가 높으리라 기대된다.

Keywords

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Fig. 1. Fruiting bodies of the wild mushrooms in this study (A, OK795; B, OK804; C, OK811; D, OK825; E, OK826; F, OK829; G, OK832; H, OK904; I, OK943; J, OK944).

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Fig. 2. DPPH radical scavenging activities of wild mushroom extracts (1mg/ml concentrations). White bar indicates a positive control. The results are represented by the mean ± S.D. of values obtained from three replications (OK795, Amanita manginiana; OK804, Suillus granulatus; OK811, Leucopaxillus giganteus; OK825, Clitocybe robusta; OK826, Hypholoma fasciculare; OK829, L. giganteus; OK832, Leucopaxillus sp.; OK904, Lactarius chrysorrheus; OK943, Pholiota limonella; OK944, A. manginiana). Different letters are significantly different by Duncan’s multiple range test (p<0.05).

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Fig. 3. Nitrite scavenging activities of wild mushroom extracts (1mg/ml concentrations). White bar indicates a positive control. The results are represented by the mean ± S.D. of values obtained from three replications (OK795, Amanita manginiana; OK804, Suillus granulatus; OK811, Leucopaxillus giganteus; OK825, Clitocybe robusta; OK826, Hypholoma fasciculare; OK829, L. giganteus; OK832, Leucopaxillus sp.; OK904, Lactarius chrysorrheus; OK943, Pholiota limonella; OK944, A. manginiana). Different letters are significantly different by Duncan’s multiple range test (p<0.05).

BSHGBD_2019_v17n2_70_f0004.png 이미지

Fig. 4. Total polyphenol contents of wild mushroom extracts (1mg/ml concentrations). White bar indicates a negative control. The results are represented by the mean ± S.D. of values obtained from three replications (OK795, Amanita manginiana; OK804, Suillus granulatus; OK811, Leucopaxillus giganteus; OK825, Clitocybe robusta; OK826, Hypholoma fasciculare; OK829, L. giganteus; OK832, Leucopaxillus sp.; OK904, Lactarius chrysorrheus; OK943, Pholiota limonella; OK944, A. manginiana). Different letters are significantly different by Duncan’s multiple range test (p<0.05).

BSHGBD_2019_v17n2_70_f0005.png 이미지

Fig. 5. The suppression rates of nitric oxide (NO) by the treatment of wild mushrooms extracts were determined by using NO assay. RAW 264.7 cells were incubated under 1 μg/ml concentration of lipopolysaccharide (LPS) and wild mushrooms extracts. White bars indicate positive and negative controls. The results are represented by the mean ± S.D. of values obtained from three replications (OK795, Amanita manginiana; OK804, Suillus granulatus; OK811, Leucopaxillus giganteus; OK825, Clitocybe robusta; OK826, Hypholoma fasciculare; OK829, L. giganteus; OK832, Leucopaxillus sp.; OK904, Lactarius chrysorrheus; OK943, Pholiota limonella; OK944, A. manginiana). Different letters are significantly different by Duncan’s multiple range test (p<0.05).

BSHGBD_2019_v17n2_70_f0006.png 이미지

Fig. 6. The rates of cell viability after treating wild mushrooms extracts. Viability of RAW 264.7 cells harvest at 24 hr after 1 μg/ml concentration of LPS addition was determined using MTT assay. White bars indicate positive and negative controls. The results are represented by the mean ± S.D. of values obtained from three replications (OK795, Amanita manginiana; OK804, Suillus granulatus; OK811, Leucopaxillus giganteus; OK825, Clitocybe robusta; OK826, Hypholoma fasciculare; OK829, L. giganteus; OK832, Leucopaxillus sp.; OK904, Lactarius chrysorrheus; OK943, Pholiota limonella; OK944, A. manginiana). Different letters are significantly different by Duncan’s multiple range test (p<0.05).

Table 1. List of the strains/specimens used in this study.

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